Soil microbial communities are key factors in maintaining ecosystem multifunctionality(EMF).However,the distribution patterns of bacterial diversity and how the different bacterial taxa and their diversity dimensions ...Soil microbial communities are key factors in maintaining ecosystem multifunctionality(EMF).However,the distribution patterns of bacterial diversity and how the different bacterial taxa and their diversity dimensions affect EMF remain largely unknown.Here,we investigated variation in three measures of diversity(alpha diversity,community composition and network complexity)among rare,intermediate,and abundant taxa across a latitudinal gradient spanning five forest plots in Yunnan Province,China and examined their contributions on EMF.We aimed to characterize the diversity distributions of bacterial groups across latitudes and to assess the differences in the mechanisms underlying their contributions to EMF.We found that multifaceted diversity(i.e.,diversity assessed by the three different metrics)of rare,intermediate,and abundant bacteria generally decreased with increasing latitude.More importantly,we found that rare bacterial taxa tended to be more diverse,but they contributed less to EMF than intermediate or abundant bacteria.Among the three dimensions of diversity we assessed,only community composition significantly affected EMF across all locations,while alpha diversity had a negative effect,and network complexity showed no significant impact.Our study further emphasizes the importance of intermediate and abundant bacterial taxa as well as community composition to EMF and provides a theoretical basis for investigating the mechanisms by which belowground microorganisms drive EMF along a latitudinal gradient.展开更多
The Diaoyu Island(Diaoyu Dao)and its affiliated islands(DAA)have abundant fishery resources,and chlorophyll a(Chl a)concentration is an important marine color element and an important indicator of primary productivity...The Diaoyu Island(Diaoyu Dao)and its affiliated islands(DAA)have abundant fishery resources,and chlorophyll a(Chl a)concentration is an important marine color element and an important indicator of primary productivity in the ocean.Therefore,it is meaningful to understand the distribution and variation characteristics of Chl a concentration in the waters near the DAA.The distribution details of Chl a concentration in the adjacent waters of DAA were revealed by high-resolution satellite GF-1 Wide Field View(WFV)data with 16 m spatial resolution.The results indicate that:(1)The Chl a concentration is between 0.06μg/L and 0.38μg/L throughout the year and the concentration of Chl a in the northeast and east directions of the island(downstream)is significantly higher than that in the west(upstream),and there are observed vortexes with high Chl a concentration in the downstream of DAA.This phenomenon continues to persist in the waters surrounding the DAA all year.(2)The vortex induced by the interaction between the current and island results in the vorticity change of water,inducing the replenishment of a large number of nutrients to the surface,thereby promoting the growth of plankton in the downstream of DAA on the east.In addition,the DAA also plays a significant role in regulating the downstream mix layer depth(MLD).The MLD in the northeast downstream is considerably deeper than the upstream in winter and spring;it is deeper in the east downstream in summer and autumn,and the range of influence for the MLD is greatly larger than the size of the island itself.This shows that the disruption of the DAA on the current is substantial.In addition,the combined effects of SST and wind also play a significant role in modifying Chl a distribution.(3)Based on the analysis above,this study proposes the conception of building fishing pastures downstream of the DAA,and proposes a general migration plan in different seasons to scientifically and rationally utilize and protect the surrounding waters of DAA.展开更多
为揭示城镇化进程中生境破碎化对鸟类多样性及分布格局的影响,本研究于2017–2019年每年的4–8月使用样线法对贵州花溪大学城26块破碎化林地(面积介于0.3–290.4ha)中的鸟类群落进行了10次调查。共记录到鸟类78种,隶属于11目37科。其中...为揭示城镇化进程中生境破碎化对鸟类多样性及分布格局的影响,本研究于2017–2019年每年的4–8月使用样线法对贵州花溪大学城26块破碎化林地(面积介于0.3–290.4ha)中的鸟类群落进行了10次调查。共记录到鸟类78种,隶属于11目37科。其中,东洋界物种数占56.4%,古北界物种数占32.1%,广布种占11.5%;有中国特有种1种。剔除高空飞行、非森林鸟类及偶然出现物种后,不同斑块中的鸟类物种数介于12–55之间,平均每个斑块有23.2±10.5种。线性回归分析显示,鸟类物种丰富度与林地斑块的面积有显著相关性,斑块面积越小,鸟类物种丰富度越低;斑块隔离度对物种丰富度没有显著影响。基于物种多度分布矩阵的WNODF(weighted nestedness metric based on overlap and decreasing fill)嵌套分析显示,不同斑块中鸟类群落呈现出反嵌套结构。小斑块中鸟类物种丰富度较低可能与植物丰富度较低、食物资源稀缺和繁育条件不足有关,但短距离的隔离对鸟类迁入或扩散影响有限。环境过滤效应、种间竞争或优先效应可能导致不同斑块间存在较大的物种组成差异,从而导致反嵌套格局。因此,本研究建议在城市规划建设中应注重维持栖息地的完整性,对不同面积大小的破碎化斑块都应加以保护。展开更多
The difference of physicochemical properties among minerals in Baotou rare earth tailings is not significant,which leads to a great difficulty in separation of minerals.In this article,the process of magnetizing roast...The difference of physicochemical properties among minerals in Baotou rare earth tailings is not significant,which leads to a great difficulty in separation of minerals.In this article,the process of magnetizing roast and low-intensity magnetic separation was used to recover iron.Effect of calcination temperature,holding time and carbon/oxygen ratio on roasting efficiency was investigated.The parameters evaluating magnetizing roast efficiency and theoretical value were determined.X-ray diffraction(XRD)analysis was used to investigate the conversion of Fe phase after roasting.The results show that the best magnetizing roast conditions are calcination temperature of 650℃,holding time of 2.5 h,and carbon/oxygen molar ratio of 3.85.The best magnetization rate is 2.36,which is close to the theoretical value of 2.33.Based on experiments of low-intensity magnetic separation under different intensities,the best current intensity is 2.0 A to obtain the best separation results.Under the best condition,the concentrate grade of iron is 45.45% and the recovery of iron is 68.36%.Most of rare earth,fluorine,and phosphorus are enriched in the magnetic separation tailings.The XRD analysis shows that Fe exists in Fe2O3 before roasting and exists in Fe3O4after roasting.展开更多
The explosive spread of the 2019 novel coronavirus(COVID-19)provides a unique chance to rethink the relationship between human activity and air pollution.Though related studies have revealed substantial reductions in ...The explosive spread of the 2019 novel coronavirus(COVID-19)provides a unique chance to rethink the relationship between human activity and air pollution.Though related studies have revealed substantial reductions in primary emissions,obvious differences do exist in the responses of secondary pollutants,like ozone(0_(3))pollution.However,the regional disparities of O_(3)responses and their causes have still not been fully investigated.To better elucidate the interrelationship between anthropogenic emissions,chemical production,and meteorological conditions,O_(3)responses caused by lockdowns over different regions were comprehensively explored at a global scale.Observational signals of air-quality change were derived from multi-year surface measurements and satellite retrievals.With similar substantial drops in nitrogen dioxide(NO_(2)),ozone shows rising signals in most areas of both East Asia and Europe,even up to~14 ppb,while a non-negligible declining signal exists in North America,by about 2-4 ppb.Furthermore,the drivers behind the different O_(3)responses are discussed based on meteorological analysis and O_(3)sensitivity diagnosis.On the one hand,O_(3)responses to NO_(2)declines can be affected by the primary dependence on its precursors.On the other hand,it is also highly dependent on meteorological factors,especially temperature.Our study further highlights the great importance of taking into consideration both the regional disparities and synergistic effects of precursor reductions and meteorological influence for scientific mitigation of O_(3)pollution.展开更多
The traditional medicinal plant,and endangered species Aristolochia delavayi(Aristolochiaceae)is an endemic species in China and occurs in the warm and dry areas along the Jinsha river.It is also a specific host of th...The traditional medicinal plant,and endangered species Aristolochia delavayi(Aristolochiaceae)is an endemic species in China and occurs in the warm and dry areas along the Jinsha river.It is also a specific host of the larvae of Byasa daemonius,a vulnerable butterfly.In this study,15 pairs of polymorphic microsatellite primers of A.delavayi were designed and screened based on the Simple Sequence Repeats(SSR)loci found by using the results of genome skimming.Based on these 15 SSR markers,the genetic diversity and structure of 193 individuals from ten natural populations were analyzed in detail.In comparison to other endemic and endangered plants in the region,the population of A.delavayi possess a relatively high genetic diversity(He=0.550,I=1.112).AMOVA analysis showed that 68.4%of the total genetic diversity was within populations and 31.6%of the variation occurred among populations.There was a significant genetic differentiation among natural populations of A.delavayi detectable,with low gene flow(Nm=0.591).This might be attributed to geographical barriers and limited seed dispersal.To test the isolation by distance(IBD),we performed Mantel test,which showed a significant correlation between the geographic and genetic distances.In order to cope with the possible biases caused by IBD,we additionally performed Bayesian genetic cluster analyses and principal coordinate analysis(PCoA).The final cluster analysis revealed three groups with distinct geographical distribution.Habitat fragmentation and limited gene flow between these populations may be the main reasons for the current genetic structure.For conservation of this species,we suggest to divide its populations into three protection management units,with subsequent focus on the Yongsheng and Luquan populations which experienced a genetic bottleneck event in the past.展开更多
Mussel aquaculture and large yellow croaker aquaculture areas and their environmental characteristics in Zhoushan were analyzed using satellite data and in-situ surveys.A new two-step remote sensing method was propose...Mussel aquaculture and large yellow croaker aquaculture areas and their environmental characteristics in Zhoushan were analyzed using satellite data and in-situ surveys.A new two-step remote sensing method was proposed and applied to determine the basic environmental characteristics of the best mussel and large yellow croaker aquaculture areas.This methodology includes the first step of extraction of the location distribution and the second step of the extraction of internal environmental factors.The fishery ranching index(FRI1,FRI2)was established to extract the mussel and the large yellow croaker aquaculture area in Zhoushan,using Gaofen-1(GF-1)and Gaofen-6(GF-6)satellite data with a special resolution of 2 m.In the second step,the environmental factors such as sea surface temperature(SST),chlorophyll a(Chl-a)concentration,current and tide,suspended sediment concentration(SSC)in mussel aquaculture area and large yellow croaker aquaculture area were extracted and analyzed in detail.The results show the following three points.(1)For the extraction of the mussel aquaculture area,FRI1 and FRI2 are complementary,and the combination of FRI1 and FRI2 is suitable to extract the mussel aquaculture area.As for the large yellow croaker aquaculture area extraction,FRI2 is suitable.(2)Mussel aquaculture and the large yellow croaker aquaculture area in Zhoushan are mainly located on the side near the islands that are away from the eastern open waters.The water environment factor template suitable for mussel and large yellow croaker aquaculture was determined.(3)This two-step remote sensing method can be used for the preliminary screening of potential site selection for the mussels and large yellow croaker aquaculture area in the future.the fishery ranching index(FRI1,FRI2)in this paper can be applied to extract the mussel and large yellow croaker aquaculture areas in coastal waters around the world.展开更多
In this paper, we study non-abelian extensions of 3-Lie algebras through Maurer-Cartan elements. We show that there is a one-to-one correspondence between isomorphism classes of non-abelian extensions of 3-Lie algebra...In this paper, we study non-abelian extensions of 3-Lie algebras through Maurer-Cartan elements. We show that there is a one-to-one correspondence between isomorphism classes of non-abelian extensions of 3-Lie algebras and equivalence classes of Maurer-Cartan elements in a DGLA. The structure of the Leibniz algebra on the space of fundamental objects is also analyzed.展开更多
Polyploidization plays a crucial role in the evolution of angiosperm species.Almost all newly formed polyploids encounter genetic or epigenetic instabilities.However,the molecular mechanisms contributing to genomic in...Polyploidization plays a crucial role in the evolution of angiosperm species.Almost all newly formed polyploids encounter genetic or epigenetic instabilities.However,the molecular mechanisms contributing to genomic instability in synthetic polyploids have not been clearly elucidated.Here,we performed a comprehensive transcriptomic and methylomic analysis of natural and synthetic polyploid rapeseeds(Brassica napus).Our results showed that the CHG methylation levels of synthetic rapeseed in different genomic contexts(genes,transposon regions,and repeat regions)were signi fi cantly lower than those of natural rapeseed.The total number and length of CHG-DMRs between natural and synthetic polyploids were much greater than those of CG-DMRs and CHH-DMRs,and the genes overlapping with these CHG-DMRs were signi fi cantly enriched in DNA damage repair and nucleotide metabolism pathways.These results indicated that CHG methylation may be more sensitive than CG and CHH methylation in regulating the stability of the polyploid genome of B.napus.In addition,many genes involved in DNA damage repair,nucleotide metabolism,and cell cycle control were significantly differentially expressed between natural and synthetic rapeseeds.Our results highlight that the genes related to DNA repair and nucleotide metabolism display differential CHG methylation patterns between natural and synthetic polyploids and reveal the potential connection between the genomic instability of polyploid plants with DNA methylation defects and dysregulation of the DNA repair system.In addition,it was found that the maintenance of CHG methylation in B.napus might be partially regulated by MET1.Our study provides novel insights into the establishment and evolution of polyploid plants and offers a potentialidea for improving the genomic stability of newly formed Brassica polyploids.展开更多
The capacitance performances of poly（3,4-ethylenedioxythiophene）-poly（styrenesulfonate）（PEDOT-PSS） supramolecular hydrogels have been investigated systematically. The materials show a specific capacitance of 67 ...The capacitance performances of poly（3,4-ethylenedioxythiophene）-poly（styrenesulfonate）（PEDOT-PSS） supramolecular hydrogels have been investigated systematically. The materials show a specific capacitance of 67 F/g and display excellent rate capability at the scan rate as high as 5000 m V/s in the cyclic voltammogram measurements, accompanied by good cycle stability. On the basis of the measurements of the microscale morphologies, specific areas and electrical conductivities, the mechanisms for the improvement of the electrochemical properties are discussed and ascribed to the novel porous microstructures of the hydrogels and the synergetic effect of the rigid PEDOT and soft PSS components. Furthermore, polyaniline（PAn） is compounded with the PEDOT-PSS hydrogels through an interfacial polymerization process, endowing the hydrogel materials with a higher specific capacitance of 160 F/g at the scan rate of 5000 m V/s. The significance of this work lies in the demonstration of a novel method to solve the problems of conducting polymers in electrochemical applications.展开更多
This study aimed to evaluate fertility and onco-logic outcomes in women with complex hyperplasia(CH)or complex atypical hyperplasia(CAH)who received fertility-sparing therapy and in vitro fertili-zation(IVF).Endometri...This study aimed to evaluate fertility and onco-logic outcomes in women with complex hyperplasia(CH)or complex atypical hyperplasia(CAH)who received fertility-sparing therapy and in vitro fertili-zation(IVF).Endometrial carcinoma is the most common carcinoma of the female genital tract(Stu-bert and Gerber,2016),and is associated with endo-metrial hyperplasia(EH)resulting from long-term unopposed estrogenic stimulation of the endometrium.EH is characterized by non-physiological prolifera-tion of endometrium that results in glands with ir-regular shapes and varying sizes(Horn et al.,2007).The World Health Organization(WHO)classified it into four types:simple or complex hyperplasia with or without atypia.CH is characterized by glands with irregular outlines that demonstrate marked structural complexity and back-to-back crowding.Atypical hyperplasia designates a proliferation of glands ex-hibiting cytologic atypia,in which varying degrees of nuclear atypia and loss of polarity are present(Kur-man et al.,1985).It has been reported that high-dose progestin is safe and efficient for CAH or early-stage low-grade carcinoma for young women who desire fertility-preserving treatment(Randall and Kurman,1997).However,few studies have reported the dif-ferences of pregnancy outcomes between patients with CAH and CH,while those patients take a great proportion in people suffered from infertility.More studies about the outcome of IVF are needed.Our aim is to evaluate fertility and oncological outcomes in women with CH or CAH who received fertility-sparing therapy.展开更多
Taking the advantage of sun glitter(SG)observed from high-resolution satellites Gaofen-1(GF-1)and Gaofen-6(GF-6),a new method named Shield and Vortex 1-2(SAV1-2)for extracting spatial texture information from sun glit...Taking the advantage of sun glitter(SG)observed from high-resolution satellites Gaofen-1(GF-1)and Gaofen-6(GF-6),a new method named Shield and Vortex 1-2(SAV1-2)for extracting spatial texture information from sun glitter was established.Sea surface texture detail information around Diaoyu Dao and its affiliated islands was extracted using SAV1-2.Meanwhile,the dynamic characteristics of the water environment were analyzed by combining the water color,temperature and dynamic data.The results show the following information.(1)Stable westward shield effect and eastward Karman vortex streets,extracted from SG,appear upstream(westward)and downstream(eastward)of the Diaoyu Dao and its affiliated islands respectively.(2)The dominant direction of the Kuroshio Current in the Diaoyu Dao and its affiliated islands area is east,the Diaoyu Dao and its affiliated islands interacting with Kuroshio,inducing western shield and eastern shelter and wake.Furthermore,wave intervals of the shield in front of the island range from hundreds to thousands of metres,and extend wider than the island itself.(3)Combining with high-resolution sea surface temperature(SST)and chlorophyll a(Chl a)concentration,the directional changes of water color elements are mutually supported and proved with the stable shield(wave packet)effect in the west and vortex street effect in the east.The change of SST upstream of the island(western shield area)is not significant,the temperature in the downstream shelter area decreases slightly,and the SST in the downstream wake area shows a general trend of obvious decrease.The wake area is dominated by Karman vortex streets in shape,supplementing by vortices.The concentration of Chl a in the west shield of island is as low as that in downstream shelter area,while it increases significantly in the downstream wake area with shape in eddy or ribbon.(4)The SAV methods of extracting sea surface texture detail using SG can be widely used in different sea areas and water quality.This paper can provide reference for the protection and development of Diaoyu Dao and its affiliated islands and its surrounding waters.It is suggested that some kind of current buffer,such as marine wind farm,should be established in the western waters of Diaoyu Dao and its affiliated islands to protect the islands,and marine pasture should be developed in the downstream of eastern waters of Diaoyu Dao and its affiliated islands.展开更多
Two new 2H-pyran-2-one glucosides,cuscutarosides A(1)and B(2),and one new steroidal glucoside,7β-methoxy-β-sitosterol 3-O-β-glucopyranoside(3),together with 12 known compounds(4-15)were isolated from the whole plan...Two new 2H-pyran-2-one glucosides,cuscutarosides A(1)and B(2),and one new steroidal glucoside,7β-methoxy-β-sitosterol 3-O-β-glucopyranoside(3),together with 12 known compounds(4-15)were isolated from the whole plant of Cuscuta reflexa(Convolvulaceae)collected from Myanmar.The chemical structures of these new compounds were elucidated based on extensive spectroscopic analysis.The antiobesity activity of these isolates was evaluated using porcine pancreatic lipase(PPL),and the antiplatelet aggregation activity was screened using rabbit platelets induced by thrombin,platelet-activating factor(PAF),arachidonate(AA),or collagen.7β-Methoxy-β-sitosterol 3-O-β-glucopyranoside(3)showed weak PPL inhibitory activity.Cuscutaroside A(1),its acetylated derivative(1a),and scrophenoside B(8)showed weak inhibitory activity against rabbit platelet aggregation induced by collagen.Compound 1a also showed inhibitory activity against rabbit platelet aggregation induced by AA.展开更多
Two new amides(E)-N-cinnamoyl-2-methoxypiperidine(1)and(R)-1-(2-oxopyrrolidin-3-yl)-5,6-dihydropyridin-2(1H)-one(2),four new amide glucosides,retrofractosides A-D(3-6),and two new phenylpropanoid glucosides,retrofract...Two new amides(E)-N-cinnamoyl-2-methoxypiperidine(1)and(R)-1-(2-oxopyrrolidin-3-yl)-5,6-dihydropyridin-2(1H)-one(2),four new amide glucosides,retrofractosides A-D(3-6),and two new phenylpropanoid glucosides,retrofractosides E(7)and F(8),together with 24 known compounds(9-32)were isolated from the fruits of Piper retrofractum.The chemical structures of these new compounds were elucidated based on extensive spectroscopic analysis.All of these isolates(1-32)were evaluated for inhibitory activity against mouse platelet aggregation induced by the peptide AYPGKF-NH2.(E)-N-(Tetrahydro-2H-pyran-2-yl)cinnamamide(9)showed a weak inhibitory efect,with an inhibition ratio of 52.0%at a concentration of 150μM.展开更多
Pre-eclampsia is characterized by an excessive maternal inflammatory response.The cholinergic antiinflammatory pathway(CAP)has been shown as the efferent arm of a vagal reflex with the potential to limit inflammatory ...Pre-eclampsia is characterized by an excessive maternal inflammatory response.The cholinergic antiinflammatory pathway(CAP)has been shown as the efferent arm of a vagal reflex with the potential to limit inflammatory responses.Therefore,in this study,the CAP regulation through the nervous vagal stimulation(VNS)reduced the severity of NG-nitro-L-arginine methyl ester(L-NAME)-induced pre-eclampsia was determined in a rat model.Rats were given 125 mg/kg/day of L-NAME via subcutaneous injection on gestational day(GD)10–16.In addition,the rats were treated by active or sham electrical stimulation once a day during GD 13–19.Systolic blood pressure(SBP),urinary albumin,and pregnancy outcomes were documented for each rat.The average fetal weights and crown-rump length(CRL)as well as the placental weights of rats in both control and experimental groups were recorded onthe 13th day,16th day and 20th day of gestation.Subsequently,placentas were collected from the rats on GD20 to measure the level of cytokines.In addition,qRT-PCR and Western blot analysis were used to detect the mRNA and protein expression ofα7 nicotinic acetylcholine receptor(α7nAChR)and nuclear factor-κB(NF-κB),respectively.Immunohistochemistry assays were also carried out to determine the location and level ofα7nAChR and NF-κB in placentas.CAP regulation through the transcutaneous auricular nerve stimulation alleviated the clinical symptoms in the rats after L-NAME induction,including hypertension,proteinuria,fetal growth retardation and fetal death.In addition,TaVNS also increasedα7nAChR expression,reduced NF-κB p65 expression,and reversed LNAME-induced proinflammatory cytokines in the placenta tissues,including tumor necrosis factor-alpha(TNF-α),high mobility group box 1(HMGB-1)and interleukin-6(IL-6).The findings of this study showed that TaVNS might be used as a promising tool to attenuate pre-eclampsia-like symptoms.In addition,the protective effect of TaVNS was associated with the improvement ofα7nAChR expression and the inhibition of inflammatory reactions at the maternal-fetal interface through activating cholinergic anti-inflammation pathway.展开更多
Objective:To investigate the expression of RNF187 in hepatocellular carcinoma(HCC)and its relationship with prognosis.Methods:The mRNA and protein expressions in hepatocellular carcinoma and tumor-adjacent tissues of ...Objective:To investigate the expression of RNF187 in hepatocellular carcinoma(HCC)and its relationship with prognosis.Methods:The mRNA and protein expressions in hepatocellular carcinoma and tumor-adjacent tissues of HCC patients were determined by qRT-PCR and Western blot analysis,and RNF187 expressions in HCC were detected by immunohistochemistry.The relationship of RNF187 expression with clinicopathologic factors and prognosis in HCC patients were analyzed.Results:RNF187 expressed highly in HCC tissues compared with the corresponding adjacent liver tissues both in mRNA and protein level,and significantly higher in HCC tissue from patients with intrahepatic metastases or vascular invasion than those in HCC tissue from patients without intrahepatic metastases and vascular invasion(all P<0.05).The 5-year survival rate of the high expression group was lower than that of the low expression group(P<0.05).Cox regression analysis revealed that high RNF187 expression was independent risk factor for postoperative survival(HR=1.313,95%CI=1.128~1.537,P<0.001).Conclusion:High expression of RNF187 in HCC is closely related to tumor prognosis,which may become a new index to evaluate the clinical diagnosis and prognosis of liver cancer.展开更多
In order to improve the efficiency of water aerator,based on Venturi experimental principle in hydraulics and fluid dynamic ultrasonic generator,the inlet section,throat section,and outlet section of reed whistle ultr...In order to improve the efficiency of water aerator,based on Venturi experimental principle in hydraulics and fluid dynamic ultrasonic generator,the inlet section,throat section,and outlet section of reed whistle ultrasonic generator were designed,and the effect of the water aerator on dissolved oxygen was preliminarily studied. Results indicate that using this water aerator,the dissolved oxygen was 7. 94 mg / L,exceeding the saturation value of dissolved oxygen( 7. 82 mg / L) at current water temperature,reaching the supersaturation. Therefore,the designed water aerator will have a bright application prospect in sewage treatment,aquaculture and aerated irrigation of plants.展开更多
Aim: To systematically evaluate the effects of colostrum intervention on gas-trointestinal function and related diseases in premature infants. Methods: A randomized controlled trial was conducted in the Cochrane Libra...Aim: To systematically evaluate the effects of colostrum intervention on gas-trointestinal function and related diseases in premature infants. Methods: A randomized controlled trial was conducted in the Cochrane Library, PubMed, EBSCO, CINAHL, Embase, Medline, CBMDISC, CNKI, Wan Fang and VIP databases on the effects of colostrum and oral intervention on gastrointestinal function and related diseases in premature infants. Literature screening, quality evaluation and data extraction were conducted, and data analysis was conducted using Revman 5.3. Results: A total of 7 references were included, including 392 subjects. The combined results showed that: colostrum can effectively reduce the incidence of nosocomial infection [RR = 0.42, 95% CI (0.25, 0.73), P = 0.002]. Conclusion: Colostrums oral intervention can effectively reduce the incidence of nosocomial infection in premature infants. However, there was no advantage in feeding intolerance, necrotizing enters colitis and length of hospital stay.展开更多
Background： The 47,XYY syndrome could result in fertility problems. However, seldom studies reported comprehensive researches on the embryonic development and pregnancy outcomes of these patients. This study aimed to...Background： The 47,XYY syndrome could result in fertility problems. However, seldom studies reported comprehensive researches on the embryonic development and pregnancy outcomes of these patients. This study aimed to evaluate the clinical outcomes of nonmosaic 47,XYY patients performed with fluorescent in situ hybridization （FISH） and preimplantation genetic diagnosis （PGD） treatment. Methods： This was a retrospective study. Between January 2012 and May 2017, 51 infertile males with nonmosaic 47,XYY syndrome underwent FISH-PGD were included in the study. According to sex chromosomal FISH results, embryos were classified as normal signal, no nuclei fixed, no signal in fixed nuclei, suspensive signal, and abnormal signal groups, respectively. The incidence of each group, the fixation rate, and hybridization rate were calculated. Embryonic development and pregnancy outcomes were also analyzed. The measurement data were analyzed with Student＇s t-test. The comparison of categorical data was analyzed with the Chi-square test and Fisher＇s exact test when expected cell count was 〈5. Results： The 53 PGD cycles with 433 embryos were analyzed. The fixation rate was 89.6%, while the hybridization rate was 96.4%. There were 283 embryos with two sex chromosomal signals with clear diagnosis （65.4%）. The numbers of no nuclei fixed, no signal in fixed nuclei, suspensive signal, and abnormal signal groups were 45 （10.4%）, 14 （3.2%）, 24 （5.5%）, and 67 （15.5%）, respectively. Embryos with abnormal signals were abandoned. The number of good-quality embryos was 210 （57.4%）, including implanted embryos on day 4/day 5 a.ld cryopreserved. The rates of good-quality embryos in the no nuclei fixed （22.2%）, no signal in fixed nuclei （28.6%）, and suspensive signal groups （33.3%） were comparable （P 〉 0.05）, and were significantly lower than the normal signal group （66.4%, P 〈 0.001 ）. The clinical pregnancy rates of fresh and frozen embryos transferred cycles were 70.6% and 85.7%, respectively. Conclusions： Among embryos with a clear diagnosis of sex chromosome, about one-fifth showed abnormal signals. Embryos with two sex chromosomal signals are more likely to develop into good-quality ones. The application of the PGD by FISH may help to improve the clinical outcomes.展开更多
基金supported by the Fundamental Research Funds of Chinese Academy of Forestry(Nos.CAFYBB2022SY037,CAFYBB2021ZA002 and CAFYBB2022QC002)the Basic Research Foundation of Yunnan Province(Grant No.202201AT070264).
文摘Soil microbial communities are key factors in maintaining ecosystem multifunctionality(EMF).However,the distribution patterns of bacterial diversity and how the different bacterial taxa and their diversity dimensions affect EMF remain largely unknown.Here,we investigated variation in three measures of diversity(alpha diversity,community composition and network complexity)among rare,intermediate,and abundant taxa across a latitudinal gradient spanning five forest plots in Yunnan Province,China and examined their contributions on EMF.We aimed to characterize the diversity distributions of bacterial groups across latitudes and to assess the differences in the mechanisms underlying their contributions to EMF.We found that multifaceted diversity(i.e.,diversity assessed by the three different metrics)of rare,intermediate,and abundant bacteria generally decreased with increasing latitude.More importantly,we found that rare bacterial taxa tended to be more diverse,but they contributed less to EMF than intermediate or abundant bacteria.Among the three dimensions of diversity we assessed,only community composition significantly affected EMF across all locations,while alpha diversity had a negative effect,and network complexity showed no significant impact.Our study further emphasizes the importance of intermediate and abundant bacterial taxa as well as community composition to EMF and provides a theoretical basis for investigating the mechanisms by which belowground microorganisms drive EMF along a latitudinal gradient.
基金The Basic Public Welfare Research Program of Zhejiang Province under contract No.LGF21D010004the Key R&D Projects in Zhejiang Province under contract No.2023C03120.
文摘The Diaoyu Island(Diaoyu Dao)and its affiliated islands(DAA)have abundant fishery resources,and chlorophyll a(Chl a)concentration is an important marine color element and an important indicator of primary productivity in the ocean.Therefore,it is meaningful to understand the distribution and variation characteristics of Chl a concentration in the waters near the DAA.The distribution details of Chl a concentration in the adjacent waters of DAA were revealed by high-resolution satellite GF-1 Wide Field View(WFV)data with 16 m spatial resolution.The results indicate that:(1)The Chl a concentration is between 0.06μg/L and 0.38μg/L throughout the year and the concentration of Chl a in the northeast and east directions of the island(downstream)is significantly higher than that in the west(upstream),and there are observed vortexes with high Chl a concentration in the downstream of DAA.This phenomenon continues to persist in the waters surrounding the DAA all year.(2)The vortex induced by the interaction between the current and island results in the vorticity change of water,inducing the replenishment of a large number of nutrients to the surface,thereby promoting the growth of plankton in the downstream of DAA on the east.In addition,the DAA also plays a significant role in regulating the downstream mix layer depth(MLD).The MLD in the northeast downstream is considerably deeper than the upstream in winter and spring;it is deeper in the east downstream in summer and autumn,and the range of influence for the MLD is greatly larger than the size of the island itself.This shows that the disruption of the DAA on the current is substantial.In addition,the combined effects of SST and wind also play a significant role in modifying Chl a distribution.(3)Based on the analysis above,this study proposes the conception of building fishing pastures downstream of the DAA,and proposes a general migration plan in different seasons to scientifically and rationally utilize and protect the surrounding waters of DAA.
文摘为揭示城镇化进程中生境破碎化对鸟类多样性及分布格局的影响,本研究于2017–2019年每年的4–8月使用样线法对贵州花溪大学城26块破碎化林地(面积介于0.3–290.4ha)中的鸟类群落进行了10次调查。共记录到鸟类78种,隶属于11目37科。其中,东洋界物种数占56.4%,古北界物种数占32.1%,广布种占11.5%;有中国特有种1种。剔除高空飞行、非森林鸟类及偶然出现物种后,不同斑块中的鸟类物种数介于12–55之间,平均每个斑块有23.2±10.5种。线性回归分析显示,鸟类物种丰富度与林地斑块的面积有显著相关性,斑块面积越小,鸟类物种丰富度越低;斑块隔离度对物种丰富度没有显著影响。基于物种多度分布矩阵的WNODF(weighted nestedness metric based on overlap and decreasing fill)嵌套分析显示,不同斑块中鸟类群落呈现出反嵌套结构。小斑块中鸟类物种丰富度较低可能与植物丰富度较低、食物资源稀缺和繁育条件不足有关,但短距离的隔离对鸟类迁入或扩散影响有限。环境过滤效应、种间竞争或优先效应可能导致不同斑块间存在较大的物种组成差异,从而导致反嵌套格局。因此,本研究建议在城市规划建设中应注重维持栖息地的完整性,对不同面积大小的破碎化斑块都应加以保护。
基金supported by the Major State Basic Research Development Program of China (No.2012CBA01205)College Foundation of Ministry of Education of China (No.N110502002)National Natural Science Founda- tion of China (Nos. 50934004 and 51274061)
文摘The difference of physicochemical properties among minerals in Baotou rare earth tailings is not significant,which leads to a great difficulty in separation of minerals.In this article,the process of magnetizing roast and low-intensity magnetic separation was used to recover iron.Effect of calcination temperature,holding time and carbon/oxygen ratio on roasting efficiency was investigated.The parameters evaluating magnetizing roast efficiency and theoretical value were determined.X-ray diffraction(XRD)analysis was used to investigate the conversion of Fe phase after roasting.The results show that the best magnetizing roast conditions are calcination temperature of 650℃,holding time of 2.5 h,and carbon/oxygen molar ratio of 3.85.The best magnetization rate is 2.36,which is close to the theoretical value of 2.33.Based on experiments of low-intensity magnetic separation under different intensities,the best current intensity is 2.0 A to obtain the best separation results.Under the best condition,the concentrate grade of iron is 45.45% and the recovery of iron is 68.36%.Most of rare earth,fluorine,and phosphorus are enriched in the magnetic separation tailings.The XRD analysis shows that Fe exists in Fe2O3 before roasting and exists in Fe3O4after roasting.
基金This research was supported by the National Natural Science Foundation of China[grant numbers 91744311 and 41922038]the International Cooperation project of Jiangsu Provincial Science and Technology Agency[grant number BZ2017066].
文摘The explosive spread of the 2019 novel coronavirus(COVID-19)provides a unique chance to rethink the relationship between human activity and air pollution.Though related studies have revealed substantial reductions in primary emissions,obvious differences do exist in the responses of secondary pollutants,like ozone(0_(3))pollution.However,the regional disparities of O_(3)responses and their causes have still not been fully investigated.To better elucidate the interrelationship between anthropogenic emissions,chemical production,and meteorological conditions,O_(3)responses caused by lockdowns over different regions were comprehensively explored at a global scale.Observational signals of air-quality change were derived from multi-year surface measurements and satellite retrievals.With similar substantial drops in nitrogen dioxide(NO_(2)),ozone shows rising signals in most areas of both East Asia and Europe,even up to~14 ppb,while a non-negligible declining signal exists in North America,by about 2-4 ppb.Furthermore,the drivers behind the different O_(3)responses are discussed based on meteorological analysis and O_(3)sensitivity diagnosis.On the one hand,O_(3)responses to NO_(2)declines can be affected by the primary dependence on its precursors.On the other hand,it is also highly dependent on meteorological factors,especially temperature.Our study further highlights the great importance of taking into consideration both the regional disparities and synergistic effects of precursor reductions and meteorological influence for scientific mitigation of O_(3)pollution.
基金Support for this study was provided by grants from the NSFC(National Natural Science Foundation of China)-Yunnan Joint fund to support key projects(No.U1602264)Yunnan Ten Thousand Talents Plan Young&Elite Talent Project to G.Chen(YNWR-QNBJ-2018-017)+2 种基金NSFC(National Natural Science Foundation of China)(No.31660057)Y.P.GengScience&Technology Basic Resources Investigation Program of China for Survey and Germplasm Conservation of PSESP in Southwest China(2017FY100100)to W.B.Sun.
文摘The traditional medicinal plant,and endangered species Aristolochia delavayi(Aristolochiaceae)is an endemic species in China and occurs in the warm and dry areas along the Jinsha river.It is also a specific host of the larvae of Byasa daemonius,a vulnerable butterfly.In this study,15 pairs of polymorphic microsatellite primers of A.delavayi were designed and screened based on the Simple Sequence Repeats(SSR)loci found by using the results of genome skimming.Based on these 15 SSR markers,the genetic diversity and structure of 193 individuals from ten natural populations were analyzed in detail.In comparison to other endemic and endangered plants in the region,the population of A.delavayi possess a relatively high genetic diversity(He=0.550,I=1.112).AMOVA analysis showed that 68.4%of the total genetic diversity was within populations and 31.6%of the variation occurred among populations.There was a significant genetic differentiation among natural populations of A.delavayi detectable,with low gene flow(Nm=0.591).This might be attributed to geographical barriers and limited seed dispersal.To test the isolation by distance(IBD),we performed Mantel test,which showed a significant correlation between the geographic and genetic distances.In order to cope with the possible biases caused by IBD,we additionally performed Bayesian genetic cluster analyses and principal coordinate analysis(PCoA).The final cluster analysis revealed three groups with distinct geographical distribution.Habitat fragmentation and limited gene flow between these populations may be the main reasons for the current genetic structure.For conservation of this species,we suggest to divide its populations into three protection management units,with subsequent focus on the Yongsheng and Luquan populations which experienced a genetic bottleneck event in the past.
基金The National Key Research and Development Program of China under contract Nos 2023YFD2401900 and 2020YFD09008004the National Natural Science Foundation of China Key International(Regional)Cooperative Research Project under contract No.42020104009the Basic Public Welfare Research Program of Zhejiang Province under contract No.LGF21D010004.
文摘Mussel aquaculture and large yellow croaker aquaculture areas and their environmental characteristics in Zhoushan were analyzed using satellite data and in-situ surveys.A new two-step remote sensing method was proposed and applied to determine the basic environmental characteristics of the best mussel and large yellow croaker aquaculture areas.This methodology includes the first step of extraction of the location distribution and the second step of the extraction of internal environmental factors.The fishery ranching index(FRI1,FRI2)was established to extract the mussel and the large yellow croaker aquaculture area in Zhoushan,using Gaofen-1(GF-1)and Gaofen-6(GF-6)satellite data with a special resolution of 2 m.In the second step,the environmental factors such as sea surface temperature(SST),chlorophyll a(Chl-a)concentration,current and tide,suspended sediment concentration(SSC)in mussel aquaculture area and large yellow croaker aquaculture area were extracted and analyzed in detail.The results show the following three points.(1)For the extraction of the mussel aquaculture area,FRI1 and FRI2 are complementary,and the combination of FRI1 and FRI2 is suitable to extract the mussel aquaculture area.As for the large yellow croaker aquaculture area extraction,FRI2 is suitable.(2)Mussel aquaculture and the large yellow croaker aquaculture area in Zhoushan are mainly located on the side near the islands that are away from the eastern open waters.The water environment factor template suitable for mussel and large yellow croaker aquaculture was determined.(3)This two-step remote sensing method can be used for the preliminary screening of potential site selection for the mussels and large yellow croaker aquaculture area in the future.the fishery ranching index(FRI1,FRI2)in this paper can be applied to extract the mussel and large yellow croaker aquaculture areas in coastal waters around the world.
基金Supported by National Natural Science Foundation of China under Grant No.11471139National Natural Science Foundation of Jilin Province under Grant No.20170101050JC
文摘In this paper, we study non-abelian extensions of 3-Lie algebras through Maurer-Cartan elements. We show that there is a one-to-one correspondence between isomorphism classes of non-abelian extensions of 3-Lie algebras and equivalence classes of Maurer-Cartan elements in a DGLA. The structure of the Leibniz algebra on the space of fundamental objects is also analyzed.
基金This work was supported by grants from the National Key Research and Developme nt Program(2016YFD0101300)Major Science and Technology Program in Sichuan Province(2018NZDZX0003)Chengdu Applied Technology Research and Development Program(2018030502).
文摘Polyploidization plays a crucial role in the evolution of angiosperm species.Almost all newly formed polyploids encounter genetic or epigenetic instabilities.However,the molecular mechanisms contributing to genomic instability in synthetic polyploids have not been clearly elucidated.Here,we performed a comprehensive transcriptomic and methylomic analysis of natural and synthetic polyploid rapeseeds(Brassica napus).Our results showed that the CHG methylation levels of synthetic rapeseed in different genomic contexts(genes,transposon regions,and repeat regions)were signi fi cantly lower than those of natural rapeseed.The total number and length of CHG-DMRs between natural and synthetic polyploids were much greater than those of CG-DMRs and CHH-DMRs,and the genes overlapping with these CHG-DMRs were signi fi cantly enriched in DNA damage repair and nucleotide metabolism pathways.These results indicated that CHG methylation may be more sensitive than CG and CHH methylation in regulating the stability of the polyploid genome of B.napus.In addition,many genes involved in DNA damage repair,nucleotide metabolism,and cell cycle control were significantly differentially expressed between natural and synthetic rapeseeds.Our results highlight that the genes related to DNA repair and nucleotide metabolism display differential CHG methylation patterns between natural and synthetic polyploids and reveal the potential connection between the genomic instability of polyploid plants with DNA methylation defects and dysregulation of the DNA repair system.In addition,it was found that the maintenance of CHG methylation in B.napus might be partially regulated by MET1.Our study provides novel insights into the establishment and evolution of polyploid plants and offers a potentialidea for improving the genomic stability of newly formed Brassica polyploids.
基金financially supported by the National Natural Science Foundation of China(Nos.21174059 and 21374046)China Postdoctoral Science Foundation(No.2013M530249)+1 种基金Program for Changjiang Scholars and Innovative Research Teams in Universities,Open Project of State Key Laboratory of Superamolecular Structure and Materials(No.SKLSSM201416)the Testing Foundation of Nanjing University
文摘The capacitance performances of poly（3,4-ethylenedioxythiophene）-poly（styrenesulfonate）（PEDOT-PSS） supramolecular hydrogels have been investigated systematically. The materials show a specific capacitance of 67 F/g and display excellent rate capability at the scan rate as high as 5000 m V/s in the cyclic voltammogram measurements, accompanied by good cycle stability. On the basis of the measurements of the microscale morphologies, specific areas and electrical conductivities, the mechanisms for the improvement of the electrochemical properties are discussed and ascribed to the novel porous microstructures of the hydrogels and the synergetic effect of the rigid PEDOT and soft PSS components. Furthermore, polyaniline（PAn） is compounded with the PEDOT-PSS hydrogels through an interfacial polymerization process, endowing the hydrogel materials with a higher specific capacitance of 160 F/g at the scan rate of 5000 m V/s. The significance of this work lies in the demonstration of a novel method to solve the problems of conducting polymers in electrochemical applications.
基金Project supported by the National Natural Science Foundation of China(No.81571414)the key Research and Development Program of Shandong Province(No.2015gsf118124)the Chinese Medical Association of Clinical Medicine Research Special Funds(No.16020290645),China
文摘This study aimed to evaluate fertility and onco-logic outcomes in women with complex hyperplasia(CH)or complex atypical hyperplasia(CAH)who received fertility-sparing therapy and in vitro fertili-zation(IVF).Endometrial carcinoma is the most common carcinoma of the female genital tract(Stu-bert and Gerber,2016),and is associated with endo-metrial hyperplasia(EH)resulting from long-term unopposed estrogenic stimulation of the endometrium.EH is characterized by non-physiological prolifera-tion of endometrium that results in glands with ir-regular shapes and varying sizes(Horn et al.,2007).The World Health Organization(WHO)classified it into four types:simple or complex hyperplasia with or without atypia.CH is characterized by glands with irregular outlines that demonstrate marked structural complexity and back-to-back crowding.Atypical hyperplasia designates a proliferation of glands ex-hibiting cytologic atypia,in which varying degrees of nuclear atypia and loss of polarity are present(Kur-man et al.,1985).It has been reported that high-dose progestin is safe and efficient for CAH or early-stage low-grade carcinoma for young women who desire fertility-preserving treatment(Randall and Kurman,1997).However,few studies have reported the dif-ferences of pregnancy outcomes between patients with CAH and CH,while those patients take a great proportion in people suffered from infertility.More studies about the outcome of IVF are needed.Our aim is to evaluate fertility and oncological outcomes in women with CH or CAH who received fertility-sparing therapy.
基金The Science Foundation of Donghai Laboratory under contract No.DH-2022KF01010the Zhejiang Key Science and Technology Project under contract No.2020C02004+1 种基金the Basic Public Welfare Research Program of Zhejiang Province under contract No.LGF21D010004the Research on Group Ideological and Political Subject Teaching Model of Ocean Remote Sensing under contract No.132.
文摘Taking the advantage of sun glitter(SG)observed from high-resolution satellites Gaofen-1(GF-1)and Gaofen-6(GF-6),a new method named Shield and Vortex 1-2(SAV1-2)for extracting spatial texture information from sun glitter was established.Sea surface texture detail information around Diaoyu Dao and its affiliated islands was extracted using SAV1-2.Meanwhile,the dynamic characteristics of the water environment were analyzed by combining the water color,temperature and dynamic data.The results show the following information.(1)Stable westward shield effect and eastward Karman vortex streets,extracted from SG,appear upstream(westward)and downstream(eastward)of the Diaoyu Dao and its affiliated islands respectively.(2)The dominant direction of the Kuroshio Current in the Diaoyu Dao and its affiliated islands area is east,the Diaoyu Dao and its affiliated islands interacting with Kuroshio,inducing western shield and eastern shelter and wake.Furthermore,wave intervals of the shield in front of the island range from hundreds to thousands of metres,and extend wider than the island itself.(3)Combining with high-resolution sea surface temperature(SST)and chlorophyll a(Chl a)concentration,the directional changes of water color elements are mutually supported and proved with the stable shield(wave packet)effect in the west and vortex street effect in the east.The change of SST upstream of the island(western shield area)is not significant,the temperature in the downstream shelter area decreases slightly,and the SST in the downstream wake area shows a general trend of obvious decrease.The wake area is dominated by Karman vortex streets in shape,supplementing by vortices.The concentration of Chl a in the west shield of island is as low as that in downstream shelter area,while it increases significantly in the downstream wake area with shape in eddy or ribbon.(4)The SAV methods of extracting sea surface texture detail using SG can be widely used in different sea areas and water quality.This paper can provide reference for the protection and development of Diaoyu Dao and its affiliated islands and its surrounding waters.It is suggested that some kind of current buffer,such as marine wind farm,should be established in the western waters of Diaoyu Dao and its affiliated islands to protect the islands,and marine pasture should be developed in the downstream of eastern waters of Diaoyu Dao and its affiliated islands.
基金supported by the Southeast Asia Biodiversity Research Institute,Chinese Academy of Sciences(Grant Nos.2015CASEABRIRG001 and Y4ZK111B01)the International Partnership Program of Chinese Academy of Sciences(Grant No.153631KYSB20160004)+2 种基金the National Natural Science Foundation of China(31960480)the Joint Special Project of Local Undergraduate Universities in Yunnan Province,China(Grant No.2018FH001-024)the Second Tibetan Plateau Scientific Expedition and Research(STEP)Program of Ministry of Science and Technology of the People’s Republic of China(Grant No.2019QZKK0502).
文摘Two new 2H-pyran-2-one glucosides,cuscutarosides A(1)and B(2),and one new steroidal glucoside,7β-methoxy-β-sitosterol 3-O-β-glucopyranoside(3),together with 12 known compounds(4-15)were isolated from the whole plant of Cuscuta reflexa(Convolvulaceae)collected from Myanmar.The chemical structures of these new compounds were elucidated based on extensive spectroscopic analysis.The antiobesity activity of these isolates was evaluated using porcine pancreatic lipase(PPL),and the antiplatelet aggregation activity was screened using rabbit platelets induced by thrombin,platelet-activating factor(PAF),arachidonate(AA),or collagen.7β-Methoxy-β-sitosterol 3-O-β-glucopyranoside(3)showed weak PPL inhibitory activity.Cuscutaroside A(1),its acetylated derivative(1a),and scrophenoside B(8)showed weak inhibitory activity against rabbit platelet aggregation induced by collagen.Compound 1a also showed inhibitory activity against rabbit platelet aggregation induced by AA.
基金supported by the Southeast Asia Biodiversity Research Institute,Chinese Academy of Sciences(2015CASEABRIRG001 and Y4ZK111B01)the open foundation of Key Laboratory of Ethnomedicine(Minzu University of China),Ministry of Education(KLEM-KF2018Z01)the International Partnership Program of Chinese Academy of Sciences(153631KYSB20160004).
文摘Two new amides(E)-N-cinnamoyl-2-methoxypiperidine(1)and(R)-1-(2-oxopyrrolidin-3-yl)-5,6-dihydropyridin-2(1H)-one(2),four new amide glucosides,retrofractosides A-D(3-6),and two new phenylpropanoid glucosides,retrofractosides E(7)and F(8),together with 24 known compounds(9-32)were isolated from the fruits of Piper retrofractum.The chemical structures of these new compounds were elucidated based on extensive spectroscopic analysis.All of these isolates(1-32)were evaluated for inhibitory activity against mouse platelet aggregation induced by the peptide AYPGKF-NH2.(E)-N-(Tetrahydro-2H-pyran-2-yl)cinnamamide(9)showed a weak inhibitory efect,with an inhibition ratio of 52.0%at a concentration of 150μM.
基金Provincial Natural Science Foundation of China Hainan(819MS119).
文摘Pre-eclampsia is characterized by an excessive maternal inflammatory response.The cholinergic antiinflammatory pathway(CAP)has been shown as the efferent arm of a vagal reflex with the potential to limit inflammatory responses.Therefore,in this study,the CAP regulation through the nervous vagal stimulation(VNS)reduced the severity of NG-nitro-L-arginine methyl ester(L-NAME)-induced pre-eclampsia was determined in a rat model.Rats were given 125 mg/kg/day of L-NAME via subcutaneous injection on gestational day(GD)10–16.In addition,the rats were treated by active or sham electrical stimulation once a day during GD 13–19.Systolic blood pressure(SBP),urinary albumin,and pregnancy outcomes were documented for each rat.The average fetal weights and crown-rump length(CRL)as well as the placental weights of rats in both control and experimental groups were recorded onthe 13th day,16th day and 20th day of gestation.Subsequently,placentas were collected from the rats on GD20 to measure the level of cytokines.In addition,qRT-PCR and Western blot analysis were used to detect the mRNA and protein expression ofα7 nicotinic acetylcholine receptor(α7nAChR)and nuclear factor-κB(NF-κB),respectively.Immunohistochemistry assays were also carried out to determine the location and level ofα7nAChR and NF-κB in placentas.CAP regulation through the transcutaneous auricular nerve stimulation alleviated the clinical symptoms in the rats after L-NAME induction,including hypertension,proteinuria,fetal growth retardation and fetal death.In addition,TaVNS also increasedα7nAChR expression,reduced NF-κB p65 expression,and reversed LNAME-induced proinflammatory cytokines in the placenta tissues,including tumor necrosis factor-alpha(TNF-α),high mobility group box 1(HMGB-1)and interleukin-6(IL-6).The findings of this study showed that TaVNS might be used as a promising tool to attenuate pre-eclampsia-like symptoms.In addition,the protective effect of TaVNS was associated with the improvement ofα7nAChR expression and the inhibition of inflammatory reactions at the maternal-fetal interface through activating cholinergic anti-inflammation pathway.
基金Hainan key research and development plan(No.ZDYF2019120)National Natural Science Foundation of China(No.81660489,81260367,81160310)Natural Science Foundation of Hainan Province(No.819QN356)
文摘Objective:To investigate the expression of RNF187 in hepatocellular carcinoma(HCC)and its relationship with prognosis.Methods:The mRNA and protein expressions in hepatocellular carcinoma and tumor-adjacent tissues of HCC patients were determined by qRT-PCR and Western blot analysis,and RNF187 expressions in HCC were detected by immunohistochemistry.The relationship of RNF187 expression with clinicopathologic factors and prognosis in HCC patients were analyzed.Results:RNF187 expressed highly in HCC tissues compared with the corresponding adjacent liver tissues both in mRNA and protein level,and significantly higher in HCC tissue from patients with intrahepatic metastases or vascular invasion than those in HCC tissue from patients without intrahepatic metastases and vascular invasion(all P<0.05).The 5-year survival rate of the high expression group was lower than that of the low expression group(P<0.05).Cox regression analysis revealed that high RNF187 expression was independent risk factor for postoperative survival(HR=1.313,95%CI=1.128~1.537,P<0.001).Conclusion:High expression of RNF187 in HCC is closely related to tumor prognosis,which may become a new index to evaluate the clinical diagnosis and prognosis of liver cancer.
基金Supported by Project of National Natural Science Foundation(31272248)International Sci-tech Cooperation Program of Ministry of Education of China(2013DFG91190)
文摘In order to improve the efficiency of water aerator,based on Venturi experimental principle in hydraulics and fluid dynamic ultrasonic generator,the inlet section,throat section,and outlet section of reed whistle ultrasonic generator were designed,and the effect of the water aerator on dissolved oxygen was preliminarily studied. Results indicate that using this water aerator,the dissolved oxygen was 7. 94 mg / L,exceeding the saturation value of dissolved oxygen( 7. 82 mg / L) at current water temperature,reaching the supersaturation. Therefore,the designed water aerator will have a bright application prospect in sewage treatment,aquaculture and aerated irrigation of plants.
文摘Aim: To systematically evaluate the effects of colostrum intervention on gas-trointestinal function and related diseases in premature infants. Methods: A randomized controlled trial was conducted in the Cochrane Library, PubMed, EBSCO, CINAHL, Embase, Medline, CBMDISC, CNKI, Wan Fang and VIP databases on the effects of colostrum and oral intervention on gastrointestinal function and related diseases in premature infants. Literature screening, quality evaluation and data extraction were conducted, and data analysis was conducted using Revman 5.3. Results: A total of 7 references were included, including 392 subjects. The combined results showed that: colostrum can effectively reduce the incidence of nosocomial infection [RR = 0.42, 95% CI (0.25, 0.73), P = 0.002]. Conclusion: Colostrums oral intervention can effectively reduce the incidence of nosocomial infection in premature infants. However, there was no advantage in feeding intolerance, necrotizing enters colitis and length of hospital stay.
基金This study was supported by grants form Nationl Key Research and Development Program（No.2016YFC1000202）,National Natural Science Foundation of China（No.81671522）,and Shandong Medical and Health Science and Technology Development Plan （No.2016WS0370）.
文摘Background： The 47,XYY syndrome could result in fertility problems. However, seldom studies reported comprehensive researches on the embryonic development and pregnancy outcomes of these patients. This study aimed to evaluate the clinical outcomes of nonmosaic 47,XYY patients performed with fluorescent in situ hybridization （FISH） and preimplantation genetic diagnosis （PGD） treatment. Methods： This was a retrospective study. Between January 2012 and May 2017, 51 infertile males with nonmosaic 47,XYY syndrome underwent FISH-PGD were included in the study. According to sex chromosomal FISH results, embryos were classified as normal signal, no nuclei fixed, no signal in fixed nuclei, suspensive signal, and abnormal signal groups, respectively. The incidence of each group, the fixation rate, and hybridization rate were calculated. Embryonic development and pregnancy outcomes were also analyzed. The measurement data were analyzed with Student＇s t-test. The comparison of categorical data was analyzed with the Chi-square test and Fisher＇s exact test when expected cell count was 〈5. Results： The 53 PGD cycles with 433 embryos were analyzed. The fixation rate was 89.6%, while the hybridization rate was 96.4%. There were 283 embryos with two sex chromosomal signals with clear diagnosis （65.4%）. The numbers of no nuclei fixed, no signal in fixed nuclei, suspensive signal, and abnormal signal groups were 45 （10.4%）, 14 （3.2%）, 24 （5.5%）, and 67 （15.5%）, respectively. Embryos with abnormal signals were abandoned. The number of good-quality embryos was 210 （57.4%）, including implanted embryos on day 4/day 5 a.ld cryopreserved. The rates of good-quality embryos in the no nuclei fixed （22.2%）, no signal in fixed nuclei （28.6%）, and suspensive signal groups （33.3%） were comparable （P 〉 0.05）, and were significantly lower than the normal signal group （66.4%, P 〈 0.001 ）. The clinical pregnancy rates of fresh and frozen embryos transferred cycles were 70.6% and 85.7%, respectively. Conclusions： Among embryos with a clear diagnosis of sex chromosome, about one-fifth showed abnormal signals. Embryos with two sex chromosomal signals are more likely to develop into good-quality ones. The application of the PGD by FISH may help to improve the clinical outcomes.
