AIM:To explore risk factors for lymph node metastases in early gastric cancer(EGC) and to confirm the appropriate range of lymph node dissection.METHODS:A total of 202 patients with EGC who underwent curative gastrect...AIM:To explore risk factors for lymph node metastases in early gastric cancer(EGC) and to confirm the appropriate range of lymph node dissection.METHODS:A total of 202 patients with EGC who underwent curative gastrectomy with lymphadenectomy in the Department of Surgery,Xinhua Hospital and Ruijin Hospital of Shanghai Jiaotong University Medical School between November 2003 and July 2009,were retrospectively reviewed.Both the surgical procedure and the extent of lymph node dissection were based on the recommendations of the Japanese gastric cancer treatment guidelines.The macroscopic type was classified as elevated(type Ⅰ or Ⅱa),flat(Ⅱb),or depressed(Ⅱc or Ⅲ).Histopathologically,papillary and tubular adenocarcinomas were grouped together as differentiated adenocarcinomas,and poorly differentiated and signet-ring cell adenocarcinomas were regarded as undifferentiated adenocarcinomas.Univariate and multivariate analyses of lymph node metastases and patient and tumor characteristics were undertaken.RESULTS:The lymph node metastases rate in patients with EGC was 14.4%.Among these,the rate for mucosal cancer was 5.4%,and 8.9% for submucosal cancer.Univariate analysis showed an obvious correlation between lymph node metastases and tumor location,depth of invasion,morphological classification and venous invasion(χ 2 = 122.901,P = 0.001;χ 2 = 7.14,P = 0.008;χ 2 = 79.523,P = 0.001;χ 2 = 8.687,P = 0.003,respectively).In patients with submucosal cancers,the lymph node metastases rate in patients with venous invasion(60%,3/5) was higher than in those without invasion(20%,15/75)(χ 2 = 4.301,P = 0.038).Multivariate logistic regression analysis revealed that the depth of invasion was the only independent risk factor for lymph node metastases in EGC [P = 0.018,Exp(B) = 2.744].Among the patients with lymph node metastases,29 cases(14.4%) were at N1,seven cases were at N2(3.5%),and two cases were at N3(1.0%).Univariate analysis of variance revealed a close relationship between the depth of invasion and lymph node metastases at pN 1(P = 0.008).CONCLUSION:The depth of invasion was the only independent risk factor for lymph node metastases.Risk factors for metastases should be considered when choosing surgery for EGC.展开更多
AIM:To reveal the clinicopathological features and risk factors for lymph node metastases in gastric cardiac adenocarcinoma of male patients.METHODS:We retrospective reviewed a total of 146male and female patients wit...AIM:To reveal the clinicopathological features and risk factors for lymph node metastases in gastric cardiac adenocarcinoma of male patients.METHODS:We retrospective reviewed a total of 146male and female patients with gastric cardiac adenocarcinoma who had undergone curative gastrectomy with lymphadenectomy in the Department of Surgery,Xin Hua Hospital and Rui Jin Hospital of Shanghai Jiaotong University Medical School between November2001 and May 2012.Both the surgical procedure and extent of lymph node dissection were based on the recommendations of Japanese gastric cancer treatment guidelines.Univariate and multivariate analyses of lymph node metastases and the clinicopathological features were undertaken.RESULTS:The rate of lymph node metastases in male patients with gastric cardiac adenocarcinoma was72.1%.Univariate analysis showed an obvious correlation between lymph node metastases and tumor size,gross appearance,differentiation,pathological tumor depth,and lymphatic invasion in male patients.Multivariate logistic regression analysis revealed that tumor differentiation and pathological tumor depth were the independent risk factors for lymph node metastases in male patients.There was an obvious relationship between lymph node metastases and tumor size,gross appearance,differentiation,pathological tumor depth,lymphatic invasion at pN1and pN2,and nerve invasion at pN3in male patients.There were no significant differences in clinicopathological features or lymph node metastases between female and male patients.CONCLUSION:Tumor differentiation and tumor depth were risk factors for lymph node metastases in male patients with gastric cardiac adenocarcinoma and should be considered when choosing surgery.展开更多
AIM:To review the clinicopathological characteristics of concurrent gastrointestinal stromal tumors(GISTs) and gastric adenocarcinoma.METHODS:We retrospectively analyzed eight cases of synchronous adenocarcinoma and G...AIM:To review the clinicopathological characteristics of concurrent gastrointestinal stromal tumors(GISTs) and gastric adenocarcinoma.METHODS:We retrospectively analyzed eight cases of synchronous adenocarcinoma and GIST in the stomach that had been surgically resected with curative intent between March 2003 and December 2008 in Xinhua hospital and Ruijin hospital.The adenocarcinoma was determined to be the primary tumor based on the histological features.The GIST cells were diffusely and strongly positive for CD34 and CD117.RESULTS:The patients were six men and two women aged 47-80 years(average,68.6 years).GIST was preoperatively detected in only one patient.The average sizes of the gastric adenocarcinomas and GISTs were 6.000 ± 2.6186 cm and 1.825 ± 1.4370 cm,respectively.All GISTs were very low-or low-risk lesions that were detected during evaluation,staging,operation or follow-up for gastric adenocarcinoma.CONCLUSION:We hypothesized that the stomach was influenced by the same unknown carcinogen,resulting in a simultaneous proliferation of different cell lines(epithelial and stromal cell).展开更多
Due to the"trade-off"effect between the high water adsorption and low stability under high Relative Humidity of polymer matrix,fabrication of resistive-type polymer-based humidity sensors with a wide impedan...Due to the"trade-off"effect between the high water adsorption and low stability under high Relative Humidity of polymer matrix,fabrication of resistive-type polymer-based humidity sensors with a wide impedance response and excellent stability in high relative humidity remains a great challenge.Aim at solving that,a novel polymeric humidity sensing matrix,specifically a tadpole-shaped,polyhedral oligomeric silsesquioxane(POSS)containing block copolymers(BCPs)of POSS-poly(methyl methacrylate)-poly styrene(POSS-PMMA-SPS)were proposed.This novel BCP was synthesized using atom transfer radical polymerization(ATRP)employing a two-step approach,and following post sulfonation,a series of sulfonated BCPs(POSS-PMMA-SPS)with different sulfonation degree was obtained.The subject humidity sensors were produced using different sulfonated BCPs employing a dip-coating technique,and three wide-impedance response humidity sensors were produced.Each of these sensors exhibited an excellent humidity-sensing response of mo re than 104 within the humidity range from 11%to 95%RH.In particular,the humidity sensor S-6 that had a proper degree of sulfonation presented a relatively fast response(t90%of 11 s and 80 s in both the water adsorption and desorption processes),and superior repeatability for more than 30 days.展开更多
AIM： To compare and identify the differences in expression of retinal proteins between normal and diabetic rats, and to analyze the molecular pathogenetic mechanisms of retinal diseases caused by diabetes.METHODS： C...AIM： To compare and identify the differences in expression of retinal proteins between normal and diabetic rats, and to analyze the molecular pathogenetic mechanisms of retinal diseases caused by diabetes.METHODS： Changes in protein expression of retinal tissues from diabetic and normal rats were observed using 2-dimensional polyacrylamide gel electrophoresis （2-DE）. Some protein spots exhibiting statistically significant variations （P 〈 0.05） were selected randomly and identified by tandem mass spectrometry and analyzed by bioinformatics.RESULTS： 2-DE showed that the expression was upregulated in 5 retinal proteins, down-regulated in 23retinal proteins, and disappeared in 8 retinal proteins.Eight spots were identified from the 36 spots by tandem mass spectrometry （MS/MS） and analyzed by bioinformatics. Guanylate kinase 1, triosephosphate isomerase 1, ATP synthase subunit d, albumin and dimethylarginine dimethylaminohydrolase 2 played an important role in signal transduction. Triosephosphate isomerase 1, crystallin alpha B, ATP synthase subunit d and peroxiredoxin 6 were involved in energy metabolism of retinal tissues. Guanylate kinase 1 played an important role in photoexcitation of retinal rod photoreceptor cells.Whether crystallin beta A1 plays a role in diabetic retinas is unknown so far.CONCLUSION： There are differences in expression of retinal proteins between diabetic and normal rats.These proteins may be involved in the mechanisms and prognosis of retinal diseases caused by diabetes.展开更多
A free-standing paper-like three-dimensional graphene framework（3DGF） with orientated laminar structure and interconnected macropores, was obtained by the hard template-directed ordered assembly. As the sacrificial ...A free-standing paper-like three-dimensional graphene framework（3DGF） with orientated laminar structure and interconnected macropores, was obtained by the hard template-directed ordered assembly. As the sacrificial templates, polystyrene（PS） latex spheres were assembled with graphene oxide（GO） to build up a sandwich type composite film, followed by heat removal of which with a simultaneous reduction of GO. The 3DGF exhibited high specific surface area of 402.5 m2/g, controllable pores and mechanical flexibility, which was employed as the binder-free supercapacitor electrode and shows high specific gravimetric capacitance of 95 F/g at 0.5 A/g, with enhanced rate capability in 3 electrode KOH system.展开更多
Benefiting from its surface-rich functional groups,eco-friendliness,impressive electrochemical properties,excellent light absorption,structural tunability at the atomic/morphological level,and ultra-high stability und...Benefiting from its surface-rich functional groups,eco-friendliness,impressive electrochemical properties,excellent light absorption,structural tunability at the atomic/morphological level,and ultra-high stability under harsh conditions,nanodiamond has emerged as a promising carbon-based non-metallic material in the field of energy conversion such as electrocatalysis and photocatalysis.Furthermore,nanodiamond,as a new generation of green catalysts,can overcome the poisoning of catalysts by complex pollutants in advanced oxidation processes,thus effectively removing organic matter from water,which is unparalleled in reducing the cost of water purification and avoiding secondary cross-contamination of water by traditional heavy metal-based catalysts.Here,we review the research and development of nanodiamonds as major electrocatalysts and photocatalysts for energy conversion and for air/water treatment for environmental remediation.The relevant properties,trimming strategy,mechanistic understanding,and design principles of nanodiamond as a catalyst are described,as well as the challenges and prospects of this emerging field.展开更多
The integrated curriculum is an important direction of the medical medical education reform under new situation and challenges of medical development.Shanghai Jiao Tong University School of Medicine has implemented th...The integrated curriculum is an important direction of the medical medical education reform under new situation and challenges of medical development.Shanghai Jiao Tong University School of Medicine has implemented the integrated curriculum reform in recent years.The endocrine system course is one of important intergrated courses and integrates relevant contents of multiple disciplines of basic medicine,diagnostics and medical imaging.Various teaching methods are adopted,such as classroom lecture,discussion,problembased learning,experimental lesson and clinical clerkship,etc.The teaching practice is carried on in several aspects:course arrangement,teaching methods,course website construction,teaching team construction and so on.展开更多
The biomimetic materials that replicate the mechanical gradient transitions from muscle to tendon to bone remain a significant challenge in tissue engineering,particularly through simple and environmentally friendly a...The biomimetic materials that replicate the mechanical gradient transitions from muscle to tendon to bone remain a significant challenge in tissue engineering,particularly through simple and environmentally friendly approaches.This mechanical gradient is crucial for applications such as rotator cuff and Achilles tendon repair patches,which prevent stress shielding and ensure uniform stress distribution,addressing the stress concentration issues common in traditional repairs.Here,we present a strategy that achieves high strength even at high water content,enabling programmable modulus/structural gradients with broad applicability.Using rotator cuff tendon repair as a model system,we demonstrate successful in vivo tissue regeneration with integrated real-time sensing capabilities,providing quantitative data for rehabilitation protocols.The hydrogels exhibit precisely controlled regional mechanical properties and seamless interface transitions,mimicking the hierarchical structure of native tissue.This approach not only improves healing outcomes compared to conventional methods but also establishes a quantitative standard for rehabilitation training.展开更多
Poly(ethylene)glycol(PEG)has been widely used as an antifouling coating material for nanomedicines to improve their stability and safety.However,a growing number of studies have indicated that PEG is immunogenic and c...Poly(ethylene)glycol(PEG)has been widely used as an antifouling coating material for nanomedicines to improve their stability and safety.However,a growing number of studies have indicated that PEG is immunogenic and can cause unwanted immune responses,highlighting the need to develop alternative antifouling polymers for applications in nanomedicine.In this study,we report an innovative polymer,poly(2-(methylsulfinyl)ethyl glycidyl ether)(PMSOEGE),composed of a PEG backbone structure with sulfoxide-containing side chains.We demonstrated that PMSOEGE is highly biocompatible and more hydrophilic than conventional PEG due to the presence of highly polar and hydrophilic sulfoxide structures.Furthermore,PMSOEGE exhibits a much lower association with anti-PEG antibodies,as confirmed by an in vitro competitive enzyme-linked immunosorbent assay(ELISA).We applied PMSOEGE as a coating material for iron oxide nanoparticles(IONPs)and demonstrated that the PMSOEGE-coated IONPs showed significantly lower cellular uptake by macrophages compared with PEGylated IONPs.Protein corona analysis indicated that fewer proteins were associated with IONP@PMSOEGE.The results highlight the superior antifouling properties of PMSOEGE and highlight its potential to serve as a PEG alternative for various biological applications.展开更多
Purpose We aimed to report the effectiveness and toxicity of salvage radiation for postoperative endometrial cancer(EC).Methods Between 2017 and 2022,17 post-hysterectomy EC patients with loco-regional recurrence rece...Purpose We aimed to report the effectiveness and toxicity of salvage radiation for postoperative endometrial cancer(EC).Methods Between 2017 and 2022,17 post-hysterectomy EC patients with loco-regional recurrence received salvage radiation were enrolled.The survival outcomes were progression-free survival(PFS),local control(LC),distant metastasis and overall survival(OS).The toxicity was analyzed according to the radiation dose.Results Among these participants,the most common relapse site was vaginal apex(N=10[58.8%]).Salvage radiation contained external beam radiotherapy(EBRT)alone(N=9[52.9%]),vaginal brachytherapy alone(N=2[11.8%])and combined(N=6[35.3%]).Median EQD2_10 was 44.25 Gy(interquartile range[IQR],44.25-44.25)and cumulative EQD2_10 was 44.25 Gy(IQR,44.25-68.25).After the median follow-up time of 45 months(ranged 3-64),the 4-year PFS,LC,distant metastasis and OS rate was 54.1%(95%CI,31.7%-92.2%),61.1%(95% CI,38.7%-96.4%),82.4%(95% CI,61.8%-100.0%)and 86.5%(95% CI,70.7%-100.0%),respectively.Concurrent chemotherapy demonstrated a trend in protective effect on metastasis(hazard ratio[HR]0.71[95%CI,0.06-8.0]),instead of local control(HR 5.69[95% CI,0.62-52]).There was no grade≥3 acute or late rectosigmoid or bladder toxicities.Higher pelvic dose was found among whom experienced myelosuppression(P=0.03).Conclusion Salvage radiation for loco-regional recurrence EC demonstrated promising LC and OS,with acceptable toxicity.More extensive research is needed to explore its efficacy and safety in a larger cohort.展开更多
The metal halide perovskite materials demonstrate outstanding performance in photovoltaics because of their excellent optoelectronic properties (1-7)The perovskite solar cells (PSCs) exhibiting outstanding efficiency ...The metal halide perovskite materials demonstrate outstanding performance in photovoltaics because of their excellent optoelectronic properties (1-7)The perovskite solar cells (PSCs) exhibiting outstanding efficiency [8,9], high power-per-weight [10], and excellent radiation resistance[11-13] are considered to be promising for developing the new-generation energy technology for space application.展开更多
Interferon-γ （IFN-γ） triggers macrophage for inflammation response by activating the intracellular JAK-STAT1 signaling. Suppressor of cytokine signaling 1 （SOCS1） and protein tyrosine phosphatases can negatively...Interferon-γ （IFN-γ） triggers macrophage for inflammation response by activating the intracellular JAK-STAT1 signaling. Suppressor of cytokine signaling 1 （SOCS1） and protein tyrosine phosphatases can negatively modulate IFN-γ signaling. Here, we identify a novel negative feedback loop mediated by STAT3-SOCS3, which is tightly controlled by SENP1 via de-SUMOylation of protein tyrosine phosphatase 1B （PTPIB）, in IFN-y signaling. SENP1-deficient macrophages show defects in IFN-γ signaling and M1 macrophage activation. PTP1B in SENP1-deficient macrophages is highly SUMOylated, which reduces PTP1B-induced de-phosphorylation of STAT3. Activated STAT3 then suppresses STAT1 activation via SOCS3 induction in SENP1-deficient macro- phages. Accordingly, SENP1-deficient macrophages show reduced ability to resist Listerio rnonocytogenes infection. These results reveal a crucial role of SENP1-controlled STAT1 and STAT3 balance in rnacrophage polarization.展开更多
It has been well-established that blood biomolecules deposit on the surface of nanoparticles forming a protein rich layer,known as‘‘protein corona',which directly interacts with and is recognized by cells.These ...It has been well-established that blood biomolecules deposit on the surface of nanoparticles forming a protein rich layer,known as‘‘protein corona',which directly interacts with and is recognized by cells.These interactions play a critical role in affecting nanoparticle biological properties,including biodistribution[1],pathophysiology[2],and therapeutic efficacy[3].The compositions of protein corona undergo dynamic exchange in vivo and are展开更多
Diabetes mellitus （DM） is a chronic disease which is associated with numerous serious health complications such as diabetic retinopathy, and is the leading cause of new cases of blindness in adults at the age of 20-...Diabetes mellitus （DM） is a chronic disease which is associated with numerous serious health complications such as diabetic retinopathy, and is the leading cause of new cases of blindness in adults at the age of 20-74 years old. The aim of the study was to establish and optimize a two-dimensional polyacrylamide gel electrophoresis （2-DE） technique for retina proteomics to improve the resolution and reproducibility, and to observe the proteomic changes of retinal tissues in diabetic and normal rats. Proteins were extracted from retinal tissues of normal and 8 weeks diabetic SD rats and used in two-dimensional electrophoresis. Various conditions of retina proteomic 2-DE were adjusted, optimized and protein spots of differential expression were obtained through analysis of 2-DE images with PDQuest software. By choosing appropriate sample amount, using pre-cast IPG dry strips （pH 5-8） and casting 12% equal gel, satisfactory 2-DE images of retina were obtained and a steady 2-DE technique was established. In this way, we found 36 spots in 2-DE gel of diabetic retinas that exhibited statistically significant variations, including up-regulation of 5 proteins in diabetic rat retinas, down-regulation of 23, and disappearance of 8, in comparison with normal tissues. The differences of protein expression were observed in retinas between diabetic and normal rats. Our established 2-DE technique of retina proteins could be effectively applied in proteomics of retina diseases. Cellular ＆ Molecular Immunology. 2007;4（1）：65-70.展开更多
Engineered nanomaterials(ENMs)with different topographies provide effective nano−bio interfaces for controlling the differentiation of stem cells.The interaction of stem cells with nanoscale topographies and chemical ...Engineered nanomaterials(ENMs)with different topographies provide effective nano−bio interfaces for controlling the differentiation of stem cells.The interaction of stem cells with nanoscale topographies and chemical cues in their microenvironment at the nano−bio interface can guide their fate.The use of nanotopographical cues,in particular nanorods,nanopillars,nanogrooves,nanofibers,and nanopits,as well as biochemical forces mediated factors,including growth factors,cytokines,and extracellular matrix proteins,can significantly impact stem cell differentiation.These factors were seen as very effective in determining the proliferation and spreading of stem cells.The specific outgrowth of stem cells can be decided with size variation of topographic nanomaterial along with variation in matrix stiffness and surface structure like a special arrangement.The precision chemistry enabled controlled design,synthesis,and chemical composition of ENMs can regulate stem cell behaviors.The parameters of size such as aspect ratio,diameter,and pore size of nanotopographic structures are the main factors for specific termination of stem cells.Protein corona nanoparticles(NPs)have shown a powerful facet in stem cell therapy,where combining specific proteins could facilitate a certain stem cell differentiation and cellular proliferation.Nano−bio reactions implicate the interaction between biological entities and nanoparticles,which can be used to tailor the stem cells’culmination.The ion release can also be a parameter to enhance cellular proliferation and to commit the early differentiation of stem cells.Further research is needed to fully understand the mechanisms underlying the interactions between engineered nano−bio interfaces and stem cells and to develop optimized regenerative medicine and tissue engineering designs.展开更多
基金Supported by Shanghai Jiaotong University Medical School for Scientific Research,No.09XJ21013Shanghai Health Bureau for Scientific Research,No.2010029Shanghai Science and Technology Commission for Scientific Research,No.124119a0300
文摘AIM:To explore risk factors for lymph node metastases in early gastric cancer(EGC) and to confirm the appropriate range of lymph node dissection.METHODS:A total of 202 patients with EGC who underwent curative gastrectomy with lymphadenectomy in the Department of Surgery,Xinhua Hospital and Ruijin Hospital of Shanghai Jiaotong University Medical School between November 2003 and July 2009,were retrospectively reviewed.Both the surgical procedure and the extent of lymph node dissection were based on the recommendations of the Japanese gastric cancer treatment guidelines.The macroscopic type was classified as elevated(type Ⅰ or Ⅱa),flat(Ⅱb),or depressed(Ⅱc or Ⅲ).Histopathologically,papillary and tubular adenocarcinomas were grouped together as differentiated adenocarcinomas,and poorly differentiated and signet-ring cell adenocarcinomas were regarded as undifferentiated adenocarcinomas.Univariate and multivariate analyses of lymph node metastases and patient and tumor characteristics were undertaken.RESULTS:The lymph node metastases rate in patients with EGC was 14.4%.Among these,the rate for mucosal cancer was 5.4%,and 8.9% for submucosal cancer.Univariate analysis showed an obvious correlation between lymph node metastases and tumor location,depth of invasion,morphological classification and venous invasion(χ 2 = 122.901,P = 0.001;χ 2 = 7.14,P = 0.008;χ 2 = 79.523,P = 0.001;χ 2 = 8.687,P = 0.003,respectively).In patients with submucosal cancers,the lymph node metastases rate in patients with venous invasion(60%,3/5) was higher than in those without invasion(20%,15/75)(χ 2 = 4.301,P = 0.038).Multivariate logistic regression analysis revealed that the depth of invasion was the only independent risk factor for lymph node metastases in EGC [P = 0.018,Exp(B) = 2.744].Among the patients with lymph node metastases,29 cases(14.4%) were at N1,seven cases were at N2(3.5%),and two cases were at N3(1.0%).Univariate analysis of variance revealed a close relationship between the depth of invasion and lymph node metastases at pN 1(P = 0.008).CONCLUSION:The depth of invasion was the only independent risk factor for lymph node metastases.Risk factors for metastases should be considered when choosing surgery for EGC.
基金Supported by Shanghai Jiaotong University Medical School for Scientific Research,Grants No.09XJ21013Shanghai Health Bureau for Scientific Research,Grants No.2010029 and 2010057+1 种基金Shanghai Science and Technology Commission for Scientific Research,Grants No.124119a0300Shanghai Municipal Education Commission,Grants No.12Y2034
文摘AIM:To reveal the clinicopathological features and risk factors for lymph node metastases in gastric cardiac adenocarcinoma of male patients.METHODS:We retrospective reviewed a total of 146male and female patients with gastric cardiac adenocarcinoma who had undergone curative gastrectomy with lymphadenectomy in the Department of Surgery,Xin Hua Hospital and Rui Jin Hospital of Shanghai Jiaotong University Medical School between November2001 and May 2012.Both the surgical procedure and extent of lymph node dissection were based on the recommendations of Japanese gastric cancer treatment guidelines.Univariate and multivariate analyses of lymph node metastases and the clinicopathological features were undertaken.RESULTS:The rate of lymph node metastases in male patients with gastric cardiac adenocarcinoma was72.1%.Univariate analysis showed an obvious correlation between lymph node metastases and tumor size,gross appearance,differentiation,pathological tumor depth,and lymphatic invasion in male patients.Multivariate logistic regression analysis revealed that tumor differentiation and pathological tumor depth were the independent risk factors for lymph node metastases in male patients.There was an obvious relationship between lymph node metastases and tumor size,gross appearance,differentiation,pathological tumor depth,lymphatic invasion at pN1and pN2,and nerve invasion at pN3in male patients.There were no significant differences in clinicopathological features or lymph node metastases between female and male patients.CONCLUSION:Tumor differentiation and tumor depth were risk factors for lymph node metastases in male patients with gastric cardiac adenocarcinoma and should be considered when choosing surgery.
基金Supported by Shanghai Jiaotong University Medical School for Scientific Research,No.09XJ21013Shanghai Health Bureau for Scientific Research,No.2010029Shanghai Science and Technology Commission for Scientific Research,No.124119a0300
文摘AIM:To review the clinicopathological characteristics of concurrent gastrointestinal stromal tumors(GISTs) and gastric adenocarcinoma.METHODS:We retrospectively analyzed eight cases of synchronous adenocarcinoma and GIST in the stomach that had been surgically resected with curative intent between March 2003 and December 2008 in Xinhua hospital and Ruijin hospital.The adenocarcinoma was determined to be the primary tumor based on the histological features.The GIST cells were diffusely and strongly positive for CD34 and CD117.RESULTS:The patients were six men and two women aged 47-80 years(average,68.6 years).GIST was preoperatively detected in only one patient.The average sizes of the gastric adenocarcinomas and GISTs were 6.000 ± 2.6186 cm and 1.825 ± 1.4370 cm,respectively.All GISTs were very low-or low-risk lesions that were detected during evaluation,staging,operation or follow-up for gastric adenocarcinoma.CONCLUSION:We hypothesized that the stomach was influenced by the same unknown carcinogen,resulting in a simultaneous proliferation of different cell lines(epithelial and stromal cell).
基金supported by Natural Science and Technology Agency of Shaanxi Province(No.2019KW-024)fundamentalresearch funds for the central universities(No.310201911 fz050)the Open Fund for Key Lab of Guangdong High Property and Functional Macromolecular Materials(No.20190015)。
文摘Due to the"trade-off"effect between the high water adsorption and low stability under high Relative Humidity of polymer matrix,fabrication of resistive-type polymer-based humidity sensors with a wide impedance response and excellent stability in high relative humidity remains a great challenge.Aim at solving that,a novel polymeric humidity sensing matrix,specifically a tadpole-shaped,polyhedral oligomeric silsesquioxane(POSS)containing block copolymers(BCPs)of POSS-poly(methyl methacrylate)-poly styrene(POSS-PMMA-SPS)were proposed.This novel BCP was synthesized using atom transfer radical polymerization(ATRP)employing a two-step approach,and following post sulfonation,a series of sulfonated BCPs(POSS-PMMA-SPS)with different sulfonation degree was obtained.The subject humidity sensors were produced using different sulfonated BCPs employing a dip-coating technique,and three wide-impedance response humidity sensors were produced.Each of these sensors exhibited an excellent humidity-sensing response of mo re than 104 within the humidity range from 11%to 95%RH.In particular,the humidity sensor S-6 that had a proper degree of sulfonation presented a relatively fast response(t90%of 11 s and 80 s in both the water adsorption and desorption processes),and superior repeatability for more than 30 days.
文摘AIM： To compare and identify the differences in expression of retinal proteins between normal and diabetic rats, and to analyze the molecular pathogenetic mechanisms of retinal diseases caused by diabetes.METHODS： Changes in protein expression of retinal tissues from diabetic and normal rats were observed using 2-dimensional polyacrylamide gel electrophoresis （2-DE）. Some protein spots exhibiting statistically significant variations （P 〈 0.05） were selected randomly and identified by tandem mass spectrometry and analyzed by bioinformatics.RESULTS： 2-DE showed that the expression was upregulated in 5 retinal proteins, down-regulated in 23retinal proteins, and disappeared in 8 retinal proteins.Eight spots were identified from the 36 spots by tandem mass spectrometry （MS/MS） and analyzed by bioinformatics. Guanylate kinase 1, triosephosphate isomerase 1, ATP synthase subunit d, albumin and dimethylarginine dimethylaminohydrolase 2 played an important role in signal transduction. Triosephosphate isomerase 1, crystallin alpha B, ATP synthase subunit d and peroxiredoxin 6 were involved in energy metabolism of retinal tissues. Guanylate kinase 1 played an important role in photoexcitation of retinal rod photoreceptor cells.Whether crystallin beta A1 plays a role in diabetic retinas is unknown so far.CONCLUSION： There are differences in expression of retinal proteins between diabetic and normal rats.These proteins may be involved in the mechanisms and prognosis of retinal diseases caused by diabetes.
基金financial support from the Natural Science Foundation of China(51302281 and 51402324)Natural Science Foundation of Shanxi Province(2013011012–7)
文摘A free-standing paper-like three-dimensional graphene framework（3DGF） with orientated laminar structure and interconnected macropores, was obtained by the hard template-directed ordered assembly. As the sacrificial templates, polystyrene（PS） latex spheres were assembled with graphene oxide（GO） to build up a sandwich type composite film, followed by heat removal of which with a simultaneous reduction of GO. The 3DGF exhibited high specific surface area of 402.5 m2/g, controllable pores and mechanical flexibility, which was employed as the binder-free supercapacitor electrode and shows high specific gravimetric capacitance of 95 F/g at 0.5 A/g, with enhanced rate capability in 3 electrode KOH system.
基金funded by National Natural Science Foundation of China(Nos.52102162 and 11975205)Guangdong Basic and Applied Basic Research Foundation(Nos.2022A1515011794 and 2020B1515120048)+1 种基金the Young Talents in Higher Education of Guangdong(No.2021KQNCX273)the support from Jiangsu Science and Technology Programme-YoungScholar(BK20200251)。
文摘Benefiting from its surface-rich functional groups,eco-friendliness,impressive electrochemical properties,excellent light absorption,structural tunability at the atomic/morphological level,and ultra-high stability under harsh conditions,nanodiamond has emerged as a promising carbon-based non-metallic material in the field of energy conversion such as electrocatalysis and photocatalysis.Furthermore,nanodiamond,as a new generation of green catalysts,can overcome the poisoning of catalysts by complex pollutants in advanced oxidation processes,thus effectively removing organic matter from water,which is unparalleled in reducing the cost of water purification and avoiding secondary cross-contamination of water by traditional heavy metal-based catalysts.Here,we review the research and development of nanodiamonds as major electrocatalysts and photocatalysts for energy conversion and for air/water treatment for environmental remediation.The relevant properties,trimming strategy,mechanistic understanding,and design principles of nanodiamond as a catalyst are described,as well as the challenges and prospects of this emerging field.
基金financially supported by Shanghai University Undergraduate Key Teaching Reform Project(2015)
文摘The integrated curriculum is an important direction of the medical medical education reform under new situation and challenges of medical development.Shanghai Jiao Tong University School of Medicine has implemented the integrated curriculum reform in recent years.The endocrine system course is one of important intergrated courses and integrates relevant contents of multiple disciplines of basic medicine,diagnostics and medical imaging.Various teaching methods are adopted,such as classroom lecture,discussion,problembased learning,experimental lesson and clinical clerkship,etc.The teaching practice is carried on in several aspects:course arrangement,teaching methods,course website construction,teaching team construction and so on.
基金supported by the Fundamental Research Funds for the Central Universities(No.2022JC013)National NaturalScience Foundation of China(Grant No.12204271)+1 种基金Natural Science Foundation of Shandong Province of China(No.ZR2021MH023)National Natural Science Foundation of China(No.62401343).
文摘The biomimetic materials that replicate the mechanical gradient transitions from muscle to tendon to bone remain a significant challenge in tissue engineering,particularly through simple and environmentally friendly approaches.This mechanical gradient is crucial for applications such as rotator cuff and Achilles tendon repair patches,which prevent stress shielding and ensure uniform stress distribution,addressing the stress concentration issues common in traditional repairs.Here,we present a strategy that achieves high strength even at high water content,enabling programmable modulus/structural gradients with broad applicability.Using rotator cuff tendon repair as a model system,we demonstrate successful in vivo tissue regeneration with integrated real-time sensing capabilities,providing quantitative data for rehabilitation protocols.The hydrogels exhibit precisely controlled regional mechanical properties and seamless interface transitions,mimicking the hierarchical structure of native tissue.This approach not only improves healing outcomes compared to conventional methods but also establishes a quantitative standard for rehabilitation training.
基金the Australian Research Council(DP210101496,CE140100036,CE230100017)the National Health and Medical Research Council(APP1196168,APP1158026)for financial support.
文摘Poly(ethylene)glycol(PEG)has been widely used as an antifouling coating material for nanomedicines to improve their stability and safety.However,a growing number of studies have indicated that PEG is immunogenic and can cause unwanted immune responses,highlighting the need to develop alternative antifouling polymers for applications in nanomedicine.In this study,we report an innovative polymer,poly(2-(methylsulfinyl)ethyl glycidyl ether)(PMSOEGE),composed of a PEG backbone structure with sulfoxide-containing side chains.We demonstrated that PMSOEGE is highly biocompatible and more hydrophilic than conventional PEG due to the presence of highly polar and hydrophilic sulfoxide structures.Furthermore,PMSOEGE exhibits a much lower association with anti-PEG antibodies,as confirmed by an in vitro competitive enzyme-linked immunosorbent assay(ELISA).We applied PMSOEGE as a coating material for iron oxide nanoparticles(IONPs)and demonstrated that the PMSOEGE-coated IONPs showed significantly lower cellular uptake by macrophages compared with PEGylated IONPs.Protein corona analysis indicated that fewer proteins were associated with IONP@PMSOEGE.The results highlight the superior antifouling properties of PMSOEGE and highlight its potential to serve as a PEG alternative for various biological applications.
文摘Purpose We aimed to report the effectiveness and toxicity of salvage radiation for postoperative endometrial cancer(EC).Methods Between 2017 and 2022,17 post-hysterectomy EC patients with loco-regional recurrence received salvage radiation were enrolled.The survival outcomes were progression-free survival(PFS),local control(LC),distant metastasis and overall survival(OS).The toxicity was analyzed according to the radiation dose.Results Among these participants,the most common relapse site was vaginal apex(N=10[58.8%]).Salvage radiation contained external beam radiotherapy(EBRT)alone(N=9[52.9%]),vaginal brachytherapy alone(N=2[11.8%])and combined(N=6[35.3%]).Median EQD2_10 was 44.25 Gy(interquartile range[IQR],44.25-44.25)and cumulative EQD2_10 was 44.25 Gy(IQR,44.25-68.25).After the median follow-up time of 45 months(ranged 3-64),the 4-year PFS,LC,distant metastasis and OS rate was 54.1%(95%CI,31.7%-92.2%),61.1%(95% CI,38.7%-96.4%),82.4%(95% CI,61.8%-100.0%)and 86.5%(95% CI,70.7%-100.0%),respectively.Concurrent chemotherapy demonstrated a trend in protective effect on metastasis(hazard ratio[HR]0.71[95%CI,0.06-8.0]),instead of local control(HR 5.69[95% CI,0.62-52]).There was no grade≥3 acute or late rectosigmoid or bladder toxicities.Higher pelvic dose was found among whom experienced myelosuppression(P=0.03).Conclusion Salvage radiation for loco-regional recurrence EC demonstrated promising LC and OS,with acceptable toxicity.More extensive research is needed to explore its efficacy and safety in a larger cohort.
基金supported by the National Basic Research Program of China(Grant No.2015CB932203)the National Natural Science Foundation of China(Grant Nos.61722501,and 61377025)+2 种基金the Beijing Natural Science Foundation(Grant No.4164106)the Scientific Experimental System in Near Space of Chinese Academy of Sciences(Grant No.XDA17000000)the General Financial Grant from the China Postdoctoral Science Foundation(Grant No.2017M620519)
文摘The metal halide perovskite materials demonstrate outstanding performance in photovoltaics because of their excellent optoelectronic properties (1-7)The perovskite solar cells (PSCs) exhibiting outstanding efficiency [8,9], high power-per-weight [10], and excellent radiation resistance[11-13] are considered to be promising for developing the new-generation energy technology for space application.
文摘Interferon-γ （IFN-γ） triggers macrophage for inflammation response by activating the intracellular JAK-STAT1 signaling. Suppressor of cytokine signaling 1 （SOCS1） and protein tyrosine phosphatases can negatively modulate IFN-γ signaling. Here, we identify a novel negative feedback loop mediated by STAT3-SOCS3, which is tightly controlled by SENP1 via de-SUMOylation of protein tyrosine phosphatase 1B （PTPIB）, in IFN-y signaling. SENP1-deficient macrophages show defects in IFN-γ signaling and M1 macrophage activation. PTP1B in SENP1-deficient macrophages is highly SUMOylated, which reduces PTP1B-induced de-phosphorylation of STAT3. Activated STAT3 then suppresses STAT1 activation via SOCS3 induction in SENP1-deficient macro- phages. Accordingly, SENP1-deficient macrophages show reduced ability to resist Listerio rnonocytogenes infection. These results reveal a crucial role of SENP1-controlled STAT1 and STAT3 balance in rnacrophage polarization.
基金supported by the National Basic Research Program of China(2016YFA0201600)the Chinese Academy of Sciences Key Research Program for Frontier Sciences(QYZDJ-SSSLH022),the Chinese Academy of Sciences Interdisciplinary Innovation Teamthe National Science Fund for Distinguished Young Scholars(11425520)
文摘It has been well-established that blood biomolecules deposit on the surface of nanoparticles forming a protein rich layer,known as‘‘protein corona',which directly interacts with and is recognized by cells.These interactions play a critical role in affecting nanoparticle biological properties,including biodistribution[1],pathophysiology[2],and therapeutic efficacy[3].The compositions of protein corona undergo dynamic exchange in vivo and are
文摘Diabetes mellitus （DM） is a chronic disease which is associated with numerous serious health complications such as diabetic retinopathy, and is the leading cause of new cases of blindness in adults at the age of 20-74 years old. The aim of the study was to establish and optimize a two-dimensional polyacrylamide gel electrophoresis （2-DE） technique for retina proteomics to improve the resolution and reproducibility, and to observe the proteomic changes of retinal tissues in diabetic and normal rats. Proteins were extracted from retinal tissues of normal and 8 weeks diabetic SD rats and used in two-dimensional electrophoresis. Various conditions of retina proteomic 2-DE were adjusted, optimized and protein spots of differential expression were obtained through analysis of 2-DE images with PDQuest software. By choosing appropriate sample amount, using pre-cast IPG dry strips （pH 5-8） and casting 12% equal gel, satisfactory 2-DE images of retina were obtained and a steady 2-DE technique was established. In this way, we found 36 spots in 2-DE gel of diabetic retinas that exhibited statistically significant variations, including up-regulation of 5 proteins in diabetic rat retinas, down-regulation of 23, and disappearance of 8, in comparison with normal tissues. The differences of protein expression were observed in retinas between diabetic and normal rats. Our established 2-DE technique of retina proteins could be effectively applied in proteomics of retina diseases. Cellular ＆ Molecular Immunology. 2007;4（1）：65-70.
基金the National Key R&D Program of China(2021YFA1200900,2020YFA0710700 and 2021YFE0112600)the National Natural Science Foundation of China(32071402)+1 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB36000000)the Chinese Academy of Medical Sciences(CAMS)Innovation Fund for Medical Sciences(CIFMS2019-I2M-5-018).
文摘Engineered nanomaterials(ENMs)with different topographies provide effective nano−bio interfaces for controlling the differentiation of stem cells.The interaction of stem cells with nanoscale topographies and chemical cues in their microenvironment at the nano−bio interface can guide their fate.The use of nanotopographical cues,in particular nanorods,nanopillars,nanogrooves,nanofibers,and nanopits,as well as biochemical forces mediated factors,including growth factors,cytokines,and extracellular matrix proteins,can significantly impact stem cell differentiation.These factors were seen as very effective in determining the proliferation and spreading of stem cells.The specific outgrowth of stem cells can be decided with size variation of topographic nanomaterial along with variation in matrix stiffness and surface structure like a special arrangement.The precision chemistry enabled controlled design,synthesis,and chemical composition of ENMs can regulate stem cell behaviors.The parameters of size such as aspect ratio,diameter,and pore size of nanotopographic structures are the main factors for specific termination of stem cells.Protein corona nanoparticles(NPs)have shown a powerful facet in stem cell therapy,where combining specific proteins could facilitate a certain stem cell differentiation and cellular proliferation.Nano−bio reactions implicate the interaction between biological entities and nanoparticles,which can be used to tailor the stem cells’culmination.The ion release can also be a parameter to enhance cellular proliferation and to commit the early differentiation of stem cells.Further research is needed to fully understand the mechanisms underlying the interactions between engineered nano−bio interfaces and stem cells and to develop optimized regenerative medicine and tissue engineering designs.
