The comparative metabolite fingerprinting and bioactivity of different teas viz.green tea,oolong tea,white tea,orthodox black tea,and Cut/crush,Tear and Curl(CTC)black tea as compared to unprocessed tea leaves(GTL)wer...The comparative metabolite fingerprinting and bioactivity of different teas viz.green tea,oolong tea,white tea,orthodox black tea,and Cut/crush,Tear and Curl(CTC)black tea as compared to unprocessed tea leaves(GTL)were elucidated.The fresh tender tea shoots collected from single location were processed into different tea forms and brewed homogenously.The mass fingerprinting analysis using ultra high performance liquid chromatography ion mobility quadrupole time-of-flight mass spectrometry(UHPLC-QTOF-IMS)revealed substantial variability of key metabolites among different tea brews.Statistical analysis(Venn-diagram,Heatmap and PCA)has revealed significant variation in metabolites content among different teas.Catechins and caffeine were identified and quantified in all infusions using high-pressure liquid chromatography analysis.2,2-diphenyl-1-picrylhydrazyl(DPPH)free radical scavenging assay,2,2′-azino-bis(3 ethylbenzothiazoline-6-sulphonic acid(ABTS)radical cation decolourisation assay and ferric-reducing antioxidant power(FRAP)assay unveiled significant antioxidant potential by all the infusions.The results ofα-glucosidase inhibition by different teas showed positive effects in management of postprandial hyperglycemia.The tea samples also unveiled significant results for nitric oxide inhibition in lipopolysaccharide-stimulated RAW 264.7 macrophages and cytotoxicity against A-549 and CT26.CL25 cancer cells.Hence,it has established that tea processing has substantial role on key metabolite composition and therapeutic potential of different tea infusions.展开更多
Pulse microgreens(mung bean,chickpea and lentil)were evaluated for compositional changes as a function of photoperiod.The pulse microgreens were cultivated under photoperiod of 16 and 22 h,with an optimum growing temp...Pulse microgreens(mung bean,chickpea and lentil)were evaluated for compositional changes as a function of photoperiod.The pulse microgreens were cultivated under photoperiod of 16 and 22 h,with an optimum growing temperature of 22℃ in light and 17℃ in dark in polypropylene trays containing moist cotton bed.The photoperiodic response of pulse microgreens in terms of plant height,chlorophyll content,antioxidant activity,phenolic profile,sugar profile and secondary metabolites were undertaken as the experimental factors.The targeted phenolic and sugar profile varied significantly with photoperiod.The various phenolic acids,especially chlorogenic acid and p-coumaric acid increased amongst different microgreens on extending photoperiod.The increase in sugar and their derivatives reflected the upregulation of carbohydrate metabolism under longer photoperiod.Also,the plant height,total chlorophyll content and antioxidant activity of pulse microgreen increased significantly on prolonging the photoperiod.The non-targeted analysis by UHPLC-QTOF LC/MS revealed the presence of secondary metabolites of five major classes,which were known to have beneficial effects in biological system.DIMBOA-glucoside,an organic compound was exclusively present in mung bean microgreens which further increased in longer photoperiod.The extraordinary supply of secondary metabolites makes the pulse microgreens excellent ingredient for therapeutic functional foods.展开更多
文摘The comparative metabolite fingerprinting and bioactivity of different teas viz.green tea,oolong tea,white tea,orthodox black tea,and Cut/crush,Tear and Curl(CTC)black tea as compared to unprocessed tea leaves(GTL)were elucidated.The fresh tender tea shoots collected from single location were processed into different tea forms and brewed homogenously.The mass fingerprinting analysis using ultra high performance liquid chromatography ion mobility quadrupole time-of-flight mass spectrometry(UHPLC-QTOF-IMS)revealed substantial variability of key metabolites among different tea brews.Statistical analysis(Venn-diagram,Heatmap and PCA)has revealed significant variation in metabolites content among different teas.Catechins and caffeine were identified and quantified in all infusions using high-pressure liquid chromatography analysis.2,2-diphenyl-1-picrylhydrazyl(DPPH)free radical scavenging assay,2,2′-azino-bis(3 ethylbenzothiazoline-6-sulphonic acid(ABTS)radical cation decolourisation assay and ferric-reducing antioxidant power(FRAP)assay unveiled significant antioxidant potential by all the infusions.The results ofα-glucosidase inhibition by different teas showed positive effects in management of postprandial hyperglycemia.The tea samples also unveiled significant results for nitric oxide inhibition in lipopolysaccharide-stimulated RAW 264.7 macrophages and cytotoxicity against A-549 and CT26.CL25 cancer cells.Hence,it has established that tea processing has substantial role on key metabolite composition and therapeutic potential of different tea infusions.
文摘Pulse microgreens(mung bean,chickpea and lentil)were evaluated for compositional changes as a function of photoperiod.The pulse microgreens were cultivated under photoperiod of 16 and 22 h,with an optimum growing temperature of 22℃ in light and 17℃ in dark in polypropylene trays containing moist cotton bed.The photoperiodic response of pulse microgreens in terms of plant height,chlorophyll content,antioxidant activity,phenolic profile,sugar profile and secondary metabolites were undertaken as the experimental factors.The targeted phenolic and sugar profile varied significantly with photoperiod.The various phenolic acids,especially chlorogenic acid and p-coumaric acid increased amongst different microgreens on extending photoperiod.The increase in sugar and their derivatives reflected the upregulation of carbohydrate metabolism under longer photoperiod.Also,the plant height,total chlorophyll content and antioxidant activity of pulse microgreen increased significantly on prolonging the photoperiod.The non-targeted analysis by UHPLC-QTOF LC/MS revealed the presence of secondary metabolites of five major classes,which were known to have beneficial effects in biological system.DIMBOA-glucoside,an organic compound was exclusively present in mung bean microgreens which further increased in longer photoperiod.The extraordinary supply of secondary metabolites makes the pulse microgreens excellent ingredient for therapeutic functional foods.
