Plant polyphenolic compounds in recent years have drawn considerable interest,however,polyphenolic com-pounds have low oral bioavailability,sensitivity to light,pH,and susceptibility to oxidation,which prevents their ...Plant polyphenolic compounds in recent years have drawn considerable interest,however,polyphenolic com-pounds have low oral bioavailability,sensitivity to light,pH,and susceptibility to oxidation,which prevents their application in nutraceuticals.To alleviate these constraints,effective encapsulation techniques have been developed.This study aimed to enrich polyphenols(EP)from Desmodium gangeticum leaves using Amberlite XAD-16 resin and to develop EP microspheres by extrusion method using sodium alginate as matrix.Further to assess impact of alginate encapsulation on stability of total polyphenol content,antioxidant activity and biological properties for 12-month duration.The encapsulated EP microspheres were characterized by SEM and FTIR.The encapsulated EP microspheres were also assessed for their swelling characteristics,release of polyphenols at different pH,temperatures,and time durations,and bio-accessibility by in vitro simulated gastrointestinal digestion.The results showed that EP were successfully encapsulated using extrusion method.The TPC(67.8±2.6 mg/g of GAE),antioxidant activity(177.2±4.3 mg/g of AAE)of encapsulated EP microspheres were significantly stable(p<0.05)throughout 12-month duration compared to unencapsulated EP.The encapsulated EP microspheres at pH 7.4 exhibited maximum swelling,leading to a higher release of TPC compared to pH 1.6.The bio-accessibility of encapsulated EP was increased during simulated gastrointestinal conditions.The bio-logical activities of encapsulated EP microspheres also significantly retained the stability(p<0.05)compared to unencapsulated EP for 12-month duration.The results supported that utilizing alginate matrix for encapsulation improves stability and may be a promising method for food supplementation containing natural antioxidants.展开更多
文摘Plant polyphenolic compounds in recent years have drawn considerable interest,however,polyphenolic com-pounds have low oral bioavailability,sensitivity to light,pH,and susceptibility to oxidation,which prevents their application in nutraceuticals.To alleviate these constraints,effective encapsulation techniques have been developed.This study aimed to enrich polyphenols(EP)from Desmodium gangeticum leaves using Amberlite XAD-16 resin and to develop EP microspheres by extrusion method using sodium alginate as matrix.Further to assess impact of alginate encapsulation on stability of total polyphenol content,antioxidant activity and biological properties for 12-month duration.The encapsulated EP microspheres were characterized by SEM and FTIR.The encapsulated EP microspheres were also assessed for their swelling characteristics,release of polyphenols at different pH,temperatures,and time durations,and bio-accessibility by in vitro simulated gastrointestinal digestion.The results showed that EP were successfully encapsulated using extrusion method.The TPC(67.8±2.6 mg/g of GAE),antioxidant activity(177.2±4.3 mg/g of AAE)of encapsulated EP microspheres were significantly stable(p<0.05)throughout 12-month duration compared to unencapsulated EP.The encapsulated EP microspheres at pH 7.4 exhibited maximum swelling,leading to a higher release of TPC compared to pH 1.6.The bio-accessibility of encapsulated EP was increased during simulated gastrointestinal conditions.The bio-logical activities of encapsulated EP microspheres also significantly retained the stability(p<0.05)compared to unencapsulated EP for 12-month duration.The results supported that utilizing alginate matrix for encapsulation improves stability and may be a promising method for food supplementation containing natural antioxidants.
