Per-and polyfluorinated alkyl substances(PFASs) are commonly used in industrial processes and daily life products.Because they are persistent, they accumulate in the environment, wildlife and humans.Although many stud...Per-and polyfluorinated alkyl substances(PFASs) are commonly used in industrial processes and daily life products.Because they are persistent, they accumulate in the environment, wildlife and humans.Although many studies have focused on two of the most representative PFASs, PFOS and PFOA, the potential toxicity of short-chain PFASs has not yet been given sufficient attention.We used a battery of assays to evaluate the toxicity of several four-carbon and six-carbon perfluorinated sulfonates and carboxyl acids(PFBS,PFHxS, PFBA and PFHxA), with a human mesenchymal stem cell(hMSC) system.Our results demonstrate significant cyto-and potential developmental toxicity for all the compounds analyzed, with shared but also distinct mechanisms of toxicity.Moreover, the effects of PFBS and PFHxS were stronger than those of PFBA and PFHxA, but occurred at higher doses compared to PFOS or PFOA.展开更多
In recent years,neonicotinoids(NEOs)and organophosphate esters(OPEs)have been widely used as substitutes for traditional pesticides and brominated fame-retardants,respectively.Previous studies have shown that those co...In recent years,neonicotinoids(NEOs)and organophosphate esters(OPEs)have been widely used as substitutes for traditional pesticides and brominated fame-retardants,respectively.Previous studies have shown that those compounds can be frequently detected in environmental and human samples,are able to penetrate the placental barrier,and are toxic to animals.Thus,it is reasonable to speculate that NEOs and OPEs may have potential adverse effects in humans,especially during development.We employed a human embryonic stem cell differentiation-and liver S9 fraction metabolism-based fast screening model to assess the potential embryonic toxicity of those two types of chemicals.We show that four NEO and five OPE prototypes targeted mostly ectoderm specification,as neural ectoderm and neural crest genes were down-regulated,and surface ectoderm and placode markers up-regulated.Human liver S9 fraction's treatment could generally reduce the effects of the chemicals,except in a few specific instances,indicating the liver may detoxify NEOs and OPEs.Our findings suggest that NEOs and OPEs interfere with human early embryonic development.展开更多
Correction to:Radiation Detection Technology and Methods(2024)8:1486-1495.https://doi.org/10.1007/s41605-024-00470-z.In this article Methods section of the publication,the term(beamline scientific data acquisition sys...Correction to:Radiation Detection Technology and Methods(2024)8:1486-1495.https://doi.org/10.1007/s41605-024-00470-z.In this article Methods section of the publication,the term(beamline scientific data acquisition system)is incorrectly written and redundant,it has been removed.展开更多
Due to factors such as smoking,air pollution,and an aging population,the prevention and control of chronic respiratory diseases in China are becoming more severe.Combining current epidemiological investigations,exposu...Due to factors such as smoking,air pollution,and an aging population,the prevention and control of chronic respiratory diseases in China are becoming more severe.Combining current epidemiological investigations,exposure to environmental pollutants such as atmospheric pollutants,persistent organic pollutants,and heavy metals may have adverse effects on lung development.Lung differentiation models are crucial tools for elucidating the process of lung development.Through the combined effect of various cell growth factors and small molecules,pluripotent stem cells are successively induced to the definitive endoderm,the anterior foregut endoderm,lung progenitor cells,and alveolar epithelial cells and eventually form matured lung alveolar organoids,revealing the complex mechanisms of lung tissue formation.This Review compares various differentiation schemes based on in vitro culture protocols and conducts in-depth discussions for the current research on the toxicity of environmental pollutants using lung differentiation models.It aims to offer model selection criteria for scientists with different research purposes and provide scientific evidence to further research the impact of environmental pollution on respiratory diseases,as well as to provide important references for protecting public health.展开更多
In recent years,rapid industrial development has resulted in the production and exposure of a substantial number of compounds to the human body.This has created an urgent need in environmental toxicology for models th...In recent years,rapid industrial development has resulted in the production and exposure of a substantial number of compounds to the human body.This has created an urgent need in environmental toxicology for models that are efficient,accurate,and cost-effective in evaluating the health impacts of these compounds on humans.Over the past seven decades,various cancer cell lines and immortalized cell lines have made significant contributions to the advancement of research on organ toxicity.Pluripotent stem cell technology,especially toxicological models derived from pluripotent stem cells,presents modern environmental toxicologists with high-throughput,species-relevant,and predictive options.In this comprehensive review,we assess the characteristics of representative human cancer cell lines and immortalized cell lines in environmental toxicology,as well as introduce two distinct human pluripotent stem cell types and their innovative toxicological models.We explore their applications and prospects in the field of environmental toxicology,while also addressing the readiness of in vitro models to confront the emerging challenges of the future.展开更多
Environmental toxicology focuses on the effects of environmental chemicals on human health.To ensure a comprehensive assessment of exposure risks,it is crucial to consider populations that are already affected by pree...Environmental toxicology focuses on the effects of environmental chemicals on human health.To ensure a comprehensive assessment of exposure risks,it is crucial to consider populations that are already affected by preexisting medical conditions,as they may exhibit increased sensitivity to environmental contaminants.Various disease-specific animal models have been developed to simulate a range of diseases such as cancer,diabetes,and neurodevelopmental disorders.However,a significant limitation of these models is the presence of species variations.展开更多
Purpose China spallation neutron source(CSNS)operates as a pulsed neutron source with a 25 Hz repetition rate.Precise data synchronization with the beam cycle is essential for analyzing abnormal beam status and diagno...Purpose China spallation neutron source(CSNS)operates as a pulsed neutron source with a 25 Hz repetition rate.Precise data synchronization with the beam cycle is essential for analyzing abnormal beam status and diagnosing faults at CSNS.This study aims to develop a beam-synchronized data acquisition system(BSDAQ)for the CSNS accelerator,which will be used to improve the understanding and optimization of accelerator performance.Methods To tackle the network bandwidth and data storage limitations caused by continuous full-cycle data storage,this paper introduces a BSDAQ(beamline scientific data acquisition system)that employs a time window,trigger-based data collection approach.The system can be operated in two modes:periodic trigger for regular data collection intervals and random trigger for event-driven scenarios,providing a flexible triggering mechanism for comprehensive data acquisition.Upon receiving a trigger,the system is designed to capture high-precision timestamped data segments from front-end device caches,align them precisely with the beam pulse cycle,and store the processed data in the database.Furthermore,the system provides the data access interface to facilitate subsequent offline analysis.Results and conclusion The prototype of the beam-synchronized BSDAQ has been implemented and tested at the CSNS accelerator.Some fast processes,such as intra-pulse beam chopping and the automatic recovery of the beam following the activation of the fast protection system,were documented and illustrated.These results are instrumental for refining beam control and improving the reliability of the accelerator's operation.展开更多
基金supported by the National Natural Science Foundation of China (Nos.21876197, 21577166, 21707160)the Chinese Academy of Sciences (Nos.XDB14040301, 29[2015]30, QYZDJ-SSW-DQC017)the K.C.Wong Education Foundation.
文摘Per-and polyfluorinated alkyl substances(PFASs) are commonly used in industrial processes and daily life products.Because they are persistent, they accumulate in the environment, wildlife and humans.Although many studies have focused on two of the most representative PFASs, PFOS and PFOA, the potential toxicity of short-chain PFASs has not yet been given sufficient attention.We used a battery of assays to evaluate the toxicity of several four-carbon and six-carbon perfluorinated sulfonates and carboxyl acids(PFBS,PFHxS, PFBA and PFHxA), with a human mesenchymal stem cell(hMSC) system.Our results demonstrate significant cyto-and potential developmental toxicity for all the compounds analyzed, with shared but also distinct mechanisms of toxicity.Moreover, the effects of PFBS and PFHxS were stronger than those of PFBA and PFHxA, but occurred at higher doses compared to PFOS or PFOA.
基金Supported by the Natural Science Foundation of China (No.51473115 and No.21276192)the Research Project of Chongqing Education Commission (No.KJ1500632)
基金supported by the Ministry of Science and Technology of the People’s Republic of China (No.2020YFA0907500)the National Natural Science Foundation of China (Nos.22150710514,22021003,and 22106174)+1 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences (No.XDPB200202)the Postdoc Science Foundation of China (No.2021M693322)。
文摘In recent years,neonicotinoids(NEOs)and organophosphate esters(OPEs)have been widely used as substitutes for traditional pesticides and brominated fame-retardants,respectively.Previous studies have shown that those compounds can be frequently detected in environmental and human samples,are able to penetrate the placental barrier,and are toxic to animals.Thus,it is reasonable to speculate that NEOs and OPEs may have potential adverse effects in humans,especially during development.We employed a human embryonic stem cell differentiation-and liver S9 fraction metabolism-based fast screening model to assess the potential embryonic toxicity of those two types of chemicals.We show that four NEO and five OPE prototypes targeted mostly ectoderm specification,as neural ectoderm and neural crest genes were down-regulated,and surface ectoderm and placode markers up-regulated.Human liver S9 fraction's treatment could generally reduce the effects of the chemicals,except in a few specific instances,indicating the liver may detoxify NEOs and OPEs.Our findings suggest that NEOs and OPEs interfere with human early embryonic development.
文摘Correction to:Radiation Detection Technology and Methods(2024)8:1486-1495.https://doi.org/10.1007/s41605-024-00470-z.In this article Methods section of the publication,the term(beamline scientific data acquisition system)is incorrectly written and redundant,it has been removed.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences[grant number XDB0750300]the National Natural Science Foundation of China[grant numbers 22021003,22476209 and 22193052]+1 种基金Beijing Municipal Natural Science Foundation[funding number IS23120]Beijing Overseas Talents Center High-Level Foreign Talent Project[funding number C2022001].
文摘Due to factors such as smoking,air pollution,and an aging population,the prevention and control of chronic respiratory diseases in China are becoming more severe.Combining current epidemiological investigations,exposure to environmental pollutants such as atmospheric pollutants,persistent organic pollutants,and heavy metals may have adverse effects on lung development.Lung differentiation models are crucial tools for elucidating the process of lung development.Through the combined effect of various cell growth factors and small molecules,pluripotent stem cells are successively induced to the definitive endoderm,the anterior foregut endoderm,lung progenitor cells,and alveolar epithelial cells and eventually form matured lung alveolar organoids,revealing the complex mechanisms of lung tissue formation.This Review compares various differentiation schemes based on in vitro culture protocols and conducts in-depth discussions for the current research on the toxicity of environmental pollutants using lung differentiation models.It aims to offer model selection criteria for scientists with different research purposes and provide scientific evidence to further research the impact of environmental pollution on respiratory diseases,as well as to provide important references for protecting public health.
基金supported by the National Natural Science Foundation of China(grant numbers:22021003 and 22150710514)Beijing Natural Science Foundation(funding number:IS23120)Beijing Overseas Talents Center Highlevel Foreign Talent Project(funding number:C2022001).
文摘In recent years,rapid industrial development has resulted in the production and exposure of a substantial number of compounds to the human body.This has created an urgent need in environmental toxicology for models that are efficient,accurate,and cost-effective in evaluating the health impacts of these compounds on humans.Over the past seven decades,various cancer cell lines and immortalized cell lines have made significant contributions to the advancement of research on organ toxicity.Pluripotent stem cell technology,especially toxicological models derived from pluripotent stem cells,presents modern environmental toxicologists with high-throughput,species-relevant,and predictive options.In this comprehensive review,we assess the characteristics of representative human cancer cell lines and immortalized cell lines in environmental toxicology,as well as introduce two distinct human pluripotent stem cell types and their innovative toxicological models.We explore their applications and prospects in the field of environmental toxicology,while also addressing the readiness of in vitro models to confront the emerging challenges of the future.
基金supported by Beijing Municipal Natural Science Foundation(IS23120)the National Natural Science Foundation of China(22021003 and 22150710514)Beijing Overseas Talents Center High-Level Foreign Talent Project(C2022001).
文摘Environmental toxicology focuses on the effects of environmental chemicals on human health.To ensure a comprehensive assessment of exposure risks,it is crucial to consider populations that are already affected by preexisting medical conditions,as they may exhibit increased sensitivity to environmental contaminants.Various disease-specific animal models have been developed to simulate a range of diseases such as cancer,diabetes,and neurodevelopmental disorders.However,a significant limitation of these models is the presence of species variations.
基金funded by Youth Innovation Promotion Association of the Chinese Academy of Sciences,Y9291420K2,Yuliang Zhang。
文摘Purpose China spallation neutron source(CSNS)operates as a pulsed neutron source with a 25 Hz repetition rate.Precise data synchronization with the beam cycle is essential for analyzing abnormal beam status and diagnosing faults at CSNS.This study aims to develop a beam-synchronized data acquisition system(BSDAQ)for the CSNS accelerator,which will be used to improve the understanding and optimization of accelerator performance.Methods To tackle the network bandwidth and data storage limitations caused by continuous full-cycle data storage,this paper introduces a BSDAQ(beamline scientific data acquisition system)that employs a time window,trigger-based data collection approach.The system can be operated in two modes:periodic trigger for regular data collection intervals and random trigger for event-driven scenarios,providing a flexible triggering mechanism for comprehensive data acquisition.Upon receiving a trigger,the system is designed to capture high-precision timestamped data segments from front-end device caches,align them precisely with the beam pulse cycle,and store the processed data in the database.Furthermore,the system provides the data access interface to facilitate subsequent offline analysis.Results and conclusion The prototype of the beam-synchronized BSDAQ has been implemented and tested at the CSNS accelerator.Some fast processes,such as intra-pulse beam chopping and the automatic recovery of the beam following the activation of the fast protection system,were documented and illustrated.These results are instrumental for refining beam control and improving the reliability of the accelerator's operation.
