Poloxamer 407,also known as Pluronic^(■)F127,is gaining interest in the cosmetic,biomedical and pharmaceutical fields for its biocompatibility,safety and thermo-sensitive properties.Ensuring sterility is critical in ...Poloxamer 407,also known as Pluronic^(■)F127,is gaining interest in the cosmetic,biomedical and pharmaceutical fields for its biocompatibility,safety and thermo-sensitive properties.Ensuring sterility is critical in clinical applications,and steriliza-tion is often preferred over aseptic processing.However,sterili-zation can impact the functional properties of the hydrogel.In this study,we investigate the effects of steam heat(121℃,20 min),dry heat(160℃,1 h),gamma irradiation(25 kGy)and electron beam(e-beam)irradiation(15 and 25 kGy)on a 30%w/v Poloxamer 407 hydrogel formulation.Our analysis encompasses gelling properties,pH,Fourier-transform infrared spectroscopy,gel permeation chromatography,small-angle X-ray scattering,rheology,swelling,degradation by-products and lactate dehydrogenase release of the sterilized hydrogels,comparing them to a non-sterile counterpart.We demonstrated that heat sterilization alters the hydrogel’s gelling and structural properties due to water evap-oration and oxidation under harsh temperature conditions,especially when applying the dry heat method.Gamma irradiation proved unsuitable,resulting in an acidic and cytotoxic hydrogel due to oxidative degradation.In contrast,e-beam irradiation preserves the hydrogel’s elasticity,gelling and structural properties while enhancing mechanical resilience and moderating swelling.Therefore,e-beam irradiation within the 15–25 kGy range appears to be the most suitable method for sterilizing a 30%w/v Poloxamer 407 hydrogel.展开更多
基金supported by The Research Council of Norway(grant numbers 331752,332148)and(grant number 346292 to A.D.L.).
文摘Poloxamer 407,also known as Pluronic^(■)F127,is gaining interest in the cosmetic,biomedical and pharmaceutical fields for its biocompatibility,safety and thermo-sensitive properties.Ensuring sterility is critical in clinical applications,and steriliza-tion is often preferred over aseptic processing.However,sterili-zation can impact the functional properties of the hydrogel.In this study,we investigate the effects of steam heat(121℃,20 min),dry heat(160℃,1 h),gamma irradiation(25 kGy)and electron beam(e-beam)irradiation(15 and 25 kGy)on a 30%w/v Poloxamer 407 hydrogel formulation.Our analysis encompasses gelling properties,pH,Fourier-transform infrared spectroscopy,gel permeation chromatography,small-angle X-ray scattering,rheology,swelling,degradation by-products and lactate dehydrogenase release of the sterilized hydrogels,comparing them to a non-sterile counterpart.We demonstrated that heat sterilization alters the hydrogel’s gelling and structural properties due to water evap-oration and oxidation under harsh temperature conditions,especially when applying the dry heat method.Gamma irradiation proved unsuitable,resulting in an acidic and cytotoxic hydrogel due to oxidative degradation.In contrast,e-beam irradiation preserves the hydrogel’s elasticity,gelling and structural properties while enhancing mechanical resilience and moderating swelling.Therefore,e-beam irradiation within the 15–25 kGy range appears to be the most suitable method for sterilizing a 30%w/v Poloxamer 407 hydrogel.
