<span style="font-family:Verdana;"></span><b><span style="font-family:Verdana;">Background:</span></b><span style="font-family:""><span s...<span style="font-family:Verdana;"></span><b><span style="font-family:Verdana;">Background:</span></b><span style="font-family:""><span style="font-family:Verdana;"> Sepsis has a poor prognosis for critically ill patients, even with </span><span style="font-family:Verdana;">intensive management. Early diagnosis of sepsis and detection of patients</span><span style="font-family:Verdana;"> with worsening prognosis are important for immediate intervention to improve the clinical outcome.</span><b><span style="font-family:Verdana;"> Objective:</span></b><span style="font-family:Verdana;"> To investigate serum presepsin (PS) and procalcitonin (PCT) as early diagnostic and prognostic biomarkers for sepsis in critically ill patients. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> 60 critically ill patients with sepsis were subdivided into three groups of sepsis, severe sepsis and septic shock </span><span style="font-family:Verdana;">according to Acute Physiology and Chronic Health Evaluation II (APACHEII)</span> <span style="font-family:Verdana;">and quick Sequential Organ Failure Assessment (qSOFA) scores. Patients</span><span style="font-family:Verdana;"> were compared with 20 age and sex matched controls. Serum PS and PCT were measured by enzyme linked immunosorbent assay (ELISA). </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> Serum PS and PCT levels were significantly increased in septic patients than controls, and their increase was positively correlated with progression of sepsis severity till reached the highest levels in septic shock. Receiver operating </span><span style="font-family:Verdana;">characteristic (ROC) curve for predicting sepsis revealed that PS has the</span><span style="font-family:Verdana;"> highest area under curve (AUC) (0.967) with 97.5% sensitivity, 85% specificity and cut-off of >635.5</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">pg/mL, followed by PCT that has AUC (0.946), 97.5% sensitivity, 95% specificity and cut-off of >319.7</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">pg/mL. C-reactive protein (CRP) showed the lowest AUC (0.902) with 75% sensitivity, 100% specificity and cut-off of >7 mg/L. ROC curve for predicting septic shock showed that PS has the highest AUC (0.969) with 90% sensitivity, 97.5% specificity and cut-off of >5500.6 pg/mL, followed by CRP that has AUC (0.945), 90% sensitivity, 87.5% specificity and cut-off of >63 mg/L. PCT showed the lowest AUC (0.889) with 90% sensitivity, 97.5% specificity and cut-off of >822.1 pg/mL. </span><b><span style="font-family:Verdana;">Conclusions:</span></b><span style="font-family:Verdana;"> Serum PS and PCT were promising biomarkers for early diagnosis and prognosis of sepsis in critically ill patients, but PS was superior to PCT.</span></span>展开更多
<strong>Background:</strong> Hepatitis C Virus (HCV) infection is a progressive disease that may result in chronic hepatitis, fibrosis and cirrhosis. Assessment of liver fibrosis is an essential factor in ...<strong>Background:</strong> Hepatitis C Virus (HCV) infection is a progressive disease that may result in chronic hepatitis, fibrosis and cirrhosis. Assessment of liver fibrosis is an essential factor in the management of chronic HCV. <strong>Objective:</strong> To evaluate plasma soluble Urokinase Plasminogen Activator Receptor (sUPAR) and interleukin-34 (IL-34) as serological markers of liver fibrosis in patients with chronic HCV. <strong>Methods:</strong> This case-control study enrolled 60 chronic HCV patients who were subdivided into three groups of mild, moderate and severe hepatic fibrosis depending on Fibrosis-4 score (FIB-4). Patients were compared with 20 age and sex-matched controls. Plasma sUPAR and IL-34 levels were measured by Enzyme Linked Immunosorbent Assay (ELISA). <strong>Results:</strong> Plasma sUPAR and IL-34 were significantly increased in HCV patients when compared with controls, and their increase was positively correlated with the progression of hepatic fibrosis. Plasma sUPAR and IL-34 positively correlated with Aspartate Aminotransferase (AST) and Alanine Aminotransferase (ALT), and negatively correlated with hemoglobin concentration and platelet count. The output data of Receiver Operating Characteristic (ROC) curve to differentiate patients from controls revealed that sUPAR at cut-off > 186.2 ng/L and Area Under Curve (AUC) of 0.944 had (85%) sensitivity and (100%) specificity, and IL-34 at cut off > 16.4 ng/L and AUC of 0.942 had (75%) sensitivity and (100%) specificity. The output data of ROC curve to differentiate severe from mild to moderate hepatic fibrosis patients revealed that sUPAR at cut-off > 510 ng/L and AUC of 0.837 had (80%) sensitivity and (90%) specificity. While IL-34 at cut off > 55.3 ng/L and AUC of 0.844 had (85%) sensitivity and (80%) specificity. <strong>Conclusions:</strong> Increased plasma levels of sUPAR and IL-34 in chronic HCV patients with liver fibrosis and their increase was parallel to the degree of liver fibrosis. Plasma sUPAR and IL-34 can be used as serological markers of liver fibrosis in chronic HCV patients.展开更多
文摘<span style="font-family:Verdana;"></span><b><span style="font-family:Verdana;">Background:</span></b><span style="font-family:""><span style="font-family:Verdana;"> Sepsis has a poor prognosis for critically ill patients, even with </span><span style="font-family:Verdana;">intensive management. Early diagnosis of sepsis and detection of patients</span><span style="font-family:Verdana;"> with worsening prognosis are important for immediate intervention to improve the clinical outcome.</span><b><span style="font-family:Verdana;"> Objective:</span></b><span style="font-family:Verdana;"> To investigate serum presepsin (PS) and procalcitonin (PCT) as early diagnostic and prognostic biomarkers for sepsis in critically ill patients. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> 60 critically ill patients with sepsis were subdivided into three groups of sepsis, severe sepsis and septic shock </span><span style="font-family:Verdana;">according to Acute Physiology and Chronic Health Evaluation II (APACHEII)</span> <span style="font-family:Verdana;">and quick Sequential Organ Failure Assessment (qSOFA) scores. Patients</span><span style="font-family:Verdana;"> were compared with 20 age and sex matched controls. Serum PS and PCT were measured by enzyme linked immunosorbent assay (ELISA). </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> Serum PS and PCT levels were significantly increased in septic patients than controls, and their increase was positively correlated with progression of sepsis severity till reached the highest levels in septic shock. Receiver operating </span><span style="font-family:Verdana;">characteristic (ROC) curve for predicting sepsis revealed that PS has the</span><span style="font-family:Verdana;"> highest area under curve (AUC) (0.967) with 97.5% sensitivity, 85% specificity and cut-off of >635.5</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">pg/mL, followed by PCT that has AUC (0.946), 97.5% sensitivity, 95% specificity and cut-off of >319.7</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">pg/mL. C-reactive protein (CRP) showed the lowest AUC (0.902) with 75% sensitivity, 100% specificity and cut-off of >7 mg/L. ROC curve for predicting septic shock showed that PS has the highest AUC (0.969) with 90% sensitivity, 97.5% specificity and cut-off of >5500.6 pg/mL, followed by CRP that has AUC (0.945), 90% sensitivity, 87.5% specificity and cut-off of >63 mg/L. PCT showed the lowest AUC (0.889) with 90% sensitivity, 97.5% specificity and cut-off of >822.1 pg/mL. </span><b><span style="font-family:Verdana;">Conclusions:</span></b><span style="font-family:Verdana;"> Serum PS and PCT were promising biomarkers for early diagnosis and prognosis of sepsis in critically ill patients, but PS was superior to PCT.</span></span>
文摘<strong>Background:</strong> Hepatitis C Virus (HCV) infection is a progressive disease that may result in chronic hepatitis, fibrosis and cirrhosis. Assessment of liver fibrosis is an essential factor in the management of chronic HCV. <strong>Objective:</strong> To evaluate plasma soluble Urokinase Plasminogen Activator Receptor (sUPAR) and interleukin-34 (IL-34) as serological markers of liver fibrosis in patients with chronic HCV. <strong>Methods:</strong> This case-control study enrolled 60 chronic HCV patients who were subdivided into three groups of mild, moderate and severe hepatic fibrosis depending on Fibrosis-4 score (FIB-4). Patients were compared with 20 age and sex-matched controls. Plasma sUPAR and IL-34 levels were measured by Enzyme Linked Immunosorbent Assay (ELISA). <strong>Results:</strong> Plasma sUPAR and IL-34 were significantly increased in HCV patients when compared with controls, and their increase was positively correlated with the progression of hepatic fibrosis. Plasma sUPAR and IL-34 positively correlated with Aspartate Aminotransferase (AST) and Alanine Aminotransferase (ALT), and negatively correlated with hemoglobin concentration and platelet count. The output data of Receiver Operating Characteristic (ROC) curve to differentiate patients from controls revealed that sUPAR at cut-off > 186.2 ng/L and Area Under Curve (AUC) of 0.944 had (85%) sensitivity and (100%) specificity, and IL-34 at cut off > 16.4 ng/L and AUC of 0.942 had (75%) sensitivity and (100%) specificity. The output data of ROC curve to differentiate severe from mild to moderate hepatic fibrosis patients revealed that sUPAR at cut-off > 510 ng/L and AUC of 0.837 had (80%) sensitivity and (90%) specificity. While IL-34 at cut off > 55.3 ng/L and AUC of 0.844 had (85%) sensitivity and (80%) specificity. <strong>Conclusions:</strong> Increased plasma levels of sUPAR and IL-34 in chronic HCV patients with liver fibrosis and their increase was parallel to the degree of liver fibrosis. Plasma sUPAR and IL-34 can be used as serological markers of liver fibrosis in chronic HCV patients.
