Objective:To assess the effects of azoxystrobin on sperm quality,hormone levels and testicular structure in Swiss albino mice.Methods:48 Swiss albino male mice were divided into 7 groups containing 6 mice in each grou...Objective:To assess the effects of azoxystrobin on sperm quality,hormone levels and testicular structure in Swiss albino mice.Methods:48 Swiss albino male mice were divided into 7 groups containing 6 mice in each group except Group桏in which 12 mice were taken.Group栺served as the control group and treated with vehicle(distil water for 30 and 60 days).Azoxystrobin at three different doses(125,250,500 mg/kg body weight)was orally administered to Group栻,栿and桇for 30 days and Group桋,桍and桏for 60 days.After dose completion,sperm parameters,hormonal profile and testis histology were analysed.Half animals of group桏(500 mg/kg body weight)were left untreated for next 30 days to assess the recovery from reproductive toxicity.Results:At azoxystrobin 125 and 250 mg/kg,sperm viability,count,and motility remained largely unaffected,but a significant decline was observed at azoxystrobin 500 mg/kg after 30 days.After 60 days,all dose levels led to a significant decrease in sperm parameters.Morphological abnormalities in sperm,such as globular heads,bent or coiled tails,and headless or tailless sperm,were noted to be increased in a dose dependent manner.Antioxidant parameters,serum level of reproductive hormones and steroidogenic enzymes followed a similar trend with non-significant changes at low and medium doses but a marked decline at the high dose after both 30 and 60 days of exposure.Histopathology of testis also revealed degenerative changes in seminiferous tubules and Leydig cell.Conclusions:Azoxystrobin exposure at high dose(500 mg/kg)affects sperm quality and disrupts testicular function via potentially impairing antioxidant defences,deregulating steroidogenesis in Swiss albino male mice.展开更多
基金The research was conducted without any financial support from external funding agency.
文摘Objective:To assess the effects of azoxystrobin on sperm quality,hormone levels and testicular structure in Swiss albino mice.Methods:48 Swiss albino male mice were divided into 7 groups containing 6 mice in each group except Group桏in which 12 mice were taken.Group栺served as the control group and treated with vehicle(distil water for 30 and 60 days).Azoxystrobin at three different doses(125,250,500 mg/kg body weight)was orally administered to Group栻,栿and桇for 30 days and Group桋,桍and桏for 60 days.After dose completion,sperm parameters,hormonal profile and testis histology were analysed.Half animals of group桏(500 mg/kg body weight)were left untreated for next 30 days to assess the recovery from reproductive toxicity.Results:At azoxystrobin 125 and 250 mg/kg,sperm viability,count,and motility remained largely unaffected,but a significant decline was observed at azoxystrobin 500 mg/kg after 30 days.After 60 days,all dose levels led to a significant decrease in sperm parameters.Morphological abnormalities in sperm,such as globular heads,bent or coiled tails,and headless or tailless sperm,were noted to be increased in a dose dependent manner.Antioxidant parameters,serum level of reproductive hormones and steroidogenic enzymes followed a similar trend with non-significant changes at low and medium doses but a marked decline at the high dose after both 30 and 60 days of exposure.Histopathology of testis also revealed degenerative changes in seminiferous tubules and Leydig cell.Conclusions:Azoxystrobin exposure at high dose(500 mg/kg)affects sperm quality and disrupts testicular function via potentially impairing antioxidant defences,deregulating steroidogenesis in Swiss albino male mice.
