Objective:To explore the effect of alantolactone on thioacetamide-induced liver fibrosis in mice as well as elucidate its underlying mechanism.Methods:Animals were divided into 5 groups:the control,the thioacetamide g...Objective:To explore the effect of alantolactone on thioacetamide-induced liver fibrosis in mice as well as elucidate its underlying mechanism.Methods:Animals were divided into 5 groups:the control,the thioacetamide group(150 mg/kg/twice weekly),the thioacetamide groups treated with alantolactone(5 and 10 mg/kg)or silymarin(50 mg/kg),respectively.All treatments were continued for 6 successive weeks,followed by collection of sera and tissue samples.Biochemical,histological,and immunohistochemical analyses were performed to examine the hepatoprotective effects of alantolactone.Results:Alantolactone ameliorated thioacetamide-induced hepatic impairment and prevented the rise of serum activities of liver enzymes.Its hepatoprotective effect was further confirmed by histological examinations.Moreover,alantolactone lowered the expression of transforming growth factor-beta 1 and alpha-smooth muscle actin,hydroxyproline content as well as COL1A1 mRNA expression.It restored antioxidant balance and inhibited thioacetamide-induced upregulated expression of interleukin-1 beta,interleukin-6,and tumor necrosis factor-alpha,Toll-like receptor 4(TLR4),myeloid differentiation primary response 88(MyD88),and nuclear factor kappa B(NF-κB).Conclusions:Alantolactone protects against thioacetamide-induced liver fibrosis in mice by reducing collagen deposition,oxidative stress,and inflammation.These effects are mediated,at least partly,by the inhibition of TLR4/MyD88/NF-κB axis.展开更多
Objective:To explore the effect of geraniol on cyclophosphamide-induced cardiotoxicity.Methods:Mice were divided into five groups:the control group,the cyclophosphamide group(200 mg/kg cyclophosphamide,i.p.on day 7),t...Objective:To explore the effect of geraniol on cyclophosphamide-induced cardiotoxicity.Methods:Mice were divided into five groups:the control group,the cyclophosphamide group(200 mg/kg cyclophosphamide,i.p.on day 7),the group treated with geraniol 100 and 200 mg/kg from day 1 to day 14,along with a single dose of cyclophosphamide on day 7,and the geraniol alone group(200 mg/kg geraniol from day 1 to day 14).At the end of the study,animals were sacrificed,and blood and heart were collected and analyzed for biochemical,histopathological,and immunohistochemical changes.Results:Treatment with 200 mg/kg geraniol significantly reduced the levels of cardiac injury markers,malondialdehyde,and inflammatory and apoptotic markers,while increasing antioxidant activities in mice with cyclophosphamide-induced cardiotoxicity.Moreover,it remarkably alleviated histopathological aberrations in cardiac tissue.Conclusions:Geraniol attenuates cyclophosphamide-induced cardiotoxicity via antioxidant,anti-inflammatory,and antiapoptotic effects.展开更多
文摘Objective:To explore the effect of alantolactone on thioacetamide-induced liver fibrosis in mice as well as elucidate its underlying mechanism.Methods:Animals were divided into 5 groups:the control,the thioacetamide group(150 mg/kg/twice weekly),the thioacetamide groups treated with alantolactone(5 and 10 mg/kg)or silymarin(50 mg/kg),respectively.All treatments were continued for 6 successive weeks,followed by collection of sera and tissue samples.Biochemical,histological,and immunohistochemical analyses were performed to examine the hepatoprotective effects of alantolactone.Results:Alantolactone ameliorated thioacetamide-induced hepatic impairment and prevented the rise of serum activities of liver enzymes.Its hepatoprotective effect was further confirmed by histological examinations.Moreover,alantolactone lowered the expression of transforming growth factor-beta 1 and alpha-smooth muscle actin,hydroxyproline content as well as COL1A1 mRNA expression.It restored antioxidant balance and inhibited thioacetamide-induced upregulated expression of interleukin-1 beta,interleukin-6,and tumor necrosis factor-alpha,Toll-like receptor 4(TLR4),myeloid differentiation primary response 88(MyD88),and nuclear factor kappa B(NF-κB).Conclusions:Alantolactone protects against thioacetamide-induced liver fibrosis in mice by reducing collagen deposition,oxidative stress,and inflammation.These effects are mediated,at least partly,by the inhibition of TLR4/MyD88/NF-κB axis.
基金The Deanship of Scientific Research at King Abdulaziz University,Jeddah,Saudi Arabia has funded this project under grant no.RG-21-166-43.
文摘Objective:To explore the effect of geraniol on cyclophosphamide-induced cardiotoxicity.Methods:Mice were divided into five groups:the control group,the cyclophosphamide group(200 mg/kg cyclophosphamide,i.p.on day 7),the group treated with geraniol 100 and 200 mg/kg from day 1 to day 14,along with a single dose of cyclophosphamide on day 7,and the geraniol alone group(200 mg/kg geraniol from day 1 to day 14).At the end of the study,animals were sacrificed,and blood and heart were collected and analyzed for biochemical,histopathological,and immunohistochemical changes.Results:Treatment with 200 mg/kg geraniol significantly reduced the levels of cardiac injury markers,malondialdehyde,and inflammatory and apoptotic markers,while increasing antioxidant activities in mice with cyclophosphamide-induced cardiotoxicity.Moreover,it remarkably alleviated histopathological aberrations in cardiac tissue.Conclusions:Geraniol attenuates cyclophosphamide-induced cardiotoxicity via antioxidant,anti-inflammatory,and antiapoptotic effects.
