Surfaces with hydrophilic and antimicrobial properties are very attractive for cardio-vascular device-associated applications.The aim of this study was to prepare and coat a hydrophilic polymer containing a functional...Surfaces with hydrophilic and antimicrobial properties are very attractive for cardio-vascular device-associated applications.The aim of this study was to prepare and coat a hydrophilic polymer containing a functional group capable of forming triazole functionality onto the surface of polyurethane(PU).The modified surfaces were assessed with cell adhesion,bacterial adhesion and bacterial viability.Mouse fibroblast cells(NIH-3T3)and three bacterial species were used for assessment.The results showed that the modified surface not only exhibited a significant reduction in cell adhesion with a 25%-59%decrease to mouse fibroblast but also showed a sig-nificant reduction in bacterial attachment with 26%-67%,24%-61%and 23%-57%decrease to Staphylococcus aureus,Escherichia coli and Pseudomonas aeruginosa,respectively,as compared with original PU.Furthermore,the polymer-modified surface exhibited a significant antibacterial function by inhibiting bacterial growth with reduction of 49%-84%,44%-79%and 53%-79%to S.aureus,E.coli and P.aeru-ginosa,respectively,as compared with original PU.These results indicate that covalent polymer attachment enhanced the antibacterial and antifouling properties of the PU surface.展开更多
Hydrophilic polymers are very useful in biomedical applications.In this study,biocom-patible polyethylene glycol(PEG)and polyvinylpyrrolidone(PVP)polymers end-capped with succinimidyl groups were either modified or sy...Hydrophilic polymers are very useful in biomedical applications.In this study,biocom-patible polyethylene glycol(PEG)and polyvinylpyrrolidone(PVP)polymers end-capped with succinimidyl groups were either modified or synthesised and attached to poly-vinylchloride surfaces.The modified surfaces were evaluated with cell adhesion and bacterial adhesion.3T3 mouse fibroblast cells and three bacteria species were used to evaluate surface adhesion activity.Results showed that the modified surface exhibited significantly reduced 3T3 cell adhesion with a 50%-69%decrease for PEG and a 64%-81%for PVP,as compared to unmodified polyvinylchloride.The modified surface also showed significantly reduced bacterial attachment with 22%-78%,18%-76%and 20%-75%decrease for PEG and 22%-76%,18%-76%and 20%-73%for PVP to Staphy-lococcus aureus,Escherichia coli and Pseudomonas aeruginosa,respectively,as compared to unmodified polyvinylchloride.It seems that an appropriate chain length or molecular weight(neither the longest nor the shortest chain length)determines the lowest cell and bacterial adhesion in terms of PEG.On the other hand,a mixture of polymers with different chain lengths exhibited the lowest cell and bacterial adhesion in terms of PVP.展开更多
The objective of this study was to coat negatively charged polymer brushes by a covalent bond onto the surface of polyvinylchloride using a simple conventional surface free radical polymerisation technique.The coated ...The objective of this study was to coat negatively charged polymer brushes by a covalent bond onto the surface of polyvinylchloride using a simple conventional surface free radical polymerisation technique.The coated surfaces were assessed with contact angle,protein adsorption,cell adhesion,and bacterial adhesion.Bovine serum albumin and bovine fibrinogen were used for protein adsorption evaluation.Mouse fibroblast(NIH-3T3)cells and Pseudomonas aeruginosa were used to assess surface adhesion.Results show that the surface modified with all the attached polymers exhibited significantly reduced contact angle,protein adsorption,and cell as well as bacterial adhesion among which the negatively charged polymers showed extremely low values in all the tests.The negatively charged polymer shows its contact angle at 5°as compared to 70°for original polyvinylchloride.Its bovine serum albumin,bovine fibrinogen,3T3 adhesion,and P.aeruginosa adhesion were 93%,84%,92%,and 92%lower than the original PVC,respectively.Furthermore,the polyvinylchloride surface coated with negatively charged polymer brushes exhibited a hydrogel-like property.The results indicate that coating a polyvinylchloride surface with acrylic acids using a simple surface-initiated free radical polymerisation and then converting those to negatively charged salts can be an effective and efficient route for fouling-resistant applications.展开更多
基金Indiana University-Purdue University IndianapolisMajmaah University。
文摘Surfaces with hydrophilic and antimicrobial properties are very attractive for cardio-vascular device-associated applications.The aim of this study was to prepare and coat a hydrophilic polymer containing a functional group capable of forming triazole functionality onto the surface of polyurethane(PU).The modified surfaces were assessed with cell adhesion,bacterial adhesion and bacterial viability.Mouse fibroblast cells(NIH-3T3)and three bacterial species were used for assessment.The results showed that the modified surface not only exhibited a significant reduction in cell adhesion with a 25%-59%decrease to mouse fibroblast but also showed a sig-nificant reduction in bacterial attachment with 26%-67%,24%-61%and 23%-57%decrease to Staphylococcus aureus,Escherichia coli and Pseudomonas aeruginosa,respectively,as compared with original PU.Furthermore,the polymer-modified surface exhibited a significant antibacterial function by inhibiting bacterial growth with reduction of 49%-84%,44%-79%and 53%-79%to S.aureus,E.coli and P.aeru-ginosa,respectively,as compared with original PU.These results indicate that covalent polymer attachment enhanced the antibacterial and antifouling properties of the PU surface.
文摘Hydrophilic polymers are very useful in biomedical applications.In this study,biocom-patible polyethylene glycol(PEG)and polyvinylpyrrolidone(PVP)polymers end-capped with succinimidyl groups were either modified or synthesised and attached to poly-vinylchloride surfaces.The modified surfaces were evaluated with cell adhesion and bacterial adhesion.3T3 mouse fibroblast cells and three bacteria species were used to evaluate surface adhesion activity.Results showed that the modified surface exhibited significantly reduced 3T3 cell adhesion with a 50%-69%decrease for PEG and a 64%-81%for PVP,as compared to unmodified polyvinylchloride.The modified surface also showed significantly reduced bacterial attachment with 22%-78%,18%-76%and 20%-75%decrease for PEG and 22%-76%,18%-76%and 20%-73%for PVP to Staphy-lococcus aureus,Escherichia coli and Pseudomonas aeruginosa,respectively,as compared to unmodified polyvinylchloride.It seems that an appropriate chain length or molecular weight(neither the longest nor the shortest chain length)determines the lowest cell and bacterial adhesion in terms of PEG.On the other hand,a mixture of polymers with different chain lengths exhibited the lowest cell and bacterial adhesion in terms of PVP.
基金IUPUI BBRC Research Centre Support.Mr.Rashed Almousa would like to thank the Deanship of Scientific Research at Majmaah University for supporting this work under Project Number R-2023-788.
文摘The objective of this study was to coat negatively charged polymer brushes by a covalent bond onto the surface of polyvinylchloride using a simple conventional surface free radical polymerisation technique.The coated surfaces were assessed with contact angle,protein adsorption,cell adhesion,and bacterial adhesion.Bovine serum albumin and bovine fibrinogen were used for protein adsorption evaluation.Mouse fibroblast(NIH-3T3)cells and Pseudomonas aeruginosa were used to assess surface adhesion.Results show that the surface modified with all the attached polymers exhibited significantly reduced contact angle,protein adsorption,and cell as well as bacterial adhesion among which the negatively charged polymers showed extremely low values in all the tests.The negatively charged polymer shows its contact angle at 5°as compared to 70°for original polyvinylchloride.Its bovine serum albumin,bovine fibrinogen,3T3 adhesion,and P.aeruginosa adhesion were 93%,84%,92%,and 92%lower than the original PVC,respectively.Furthermore,the polyvinylchloride surface coated with negatively charged polymer brushes exhibited a hydrogel-like property.The results indicate that coating a polyvinylchloride surface with acrylic acids using a simple surface-initiated free radical polymerisation and then converting those to negatively charged salts can be an effective and efficient route for fouling-resistant applications.
