Programmed cell death(PCD)plays a crucial role in the biological processes of living organisms and occurs in various forms,such as apoptosis,necroptosis and ferroptosis.However,traditional methods for PCD analysis are...Programmed cell death(PCD)plays a crucial role in the biological processes of living organisms and occurs in various forms,such as apoptosis,necroptosis and ferroptosis.However,traditional methods for PCD analysis are time-consuming and complex.In this paper,we propose a facile surface-enhanced Raman spectroscopy(SERS)-based strategy for the real-time analysis of three PCD patterns utilizing black phosphorus–gold nanoparticles(BP–Au NPs)as the ultrasensitive unlabeled Raman probe.BP–Au NPs,which possess excellent biocompatibility,are capable of detecting dye molecules at concentrations as low as 5×10^(-8)M and remain stable for at least one week in different physiological environments.In view of this,BP–Au NPs-based SERS technique can distinguish the tiny differences in the molecular fingerprints of cancer cells undergoing three PCD patterns(apoptosis,necroptosis and ferroptosis)triggered by doxorubicin,shikonin and erastin,respectively.We also have real-time monitoring of the intracellular molecular events during PCD,which spy the fluctuations of some typical SERS bands assigned to protein,DNA and lipid,revealing the unique phenotypic characteristics of each PCD pattern.This strategy provides a detailed and comprehensive analysis of the mechanisms of drug-induced PCD at the Raman level.展开更多
In this paper, a controllable one-step doping method has been successfully adopted in the cesium copper iodide perovskite’s luminescence, a high-quality white-light emission with Commission Internationale de l’Eclai...In this paper, a controllable one-step doping method has been successfully adopted in the cesium copper iodide perovskite’s luminescence, a high-quality white-light emission with Commission Internationale de l’Eclairage(CIE) coordinates of(0.3397, 0.3325), and a color rendering index(CRI) reaching up to 90 was realized in a convenient way. Through adding impurities into the Cs_(3)Cu_(2)I_(5) system, high efficiency and stable CsCu_(2)I_(3) was synthesized, and the coexistence of varied high luminescence phases realized the white lighting.Strikingly, blue-emitting Cs_(3)Cu_(2)I_(5) and yellow-emitting CsCu_(2)I_(3) could coexist, and their respective luminescence was not interacted in the compound, which was beneficial for acquiring a single emission and highly efficient white lighting. This work carried out a deep exploration of the Cu-based metal halides, and would be favorable to the applications of lead-free perovskites.展开更多
ZnO films containing Er and Ge nanocrystals(nc-Ge) were synthesized and their photoluminescence(PL) properties were studied. Visible and near-infrared PL intensities are found to be greatly increased in nc-Ge-containi...ZnO films containing Er and Ge nanocrystals(nc-Ge) were synthesized and their photoluminescence(PL) properties were studied. Visible and near-infrared PL intensities are found to be greatly increased in nc-Ge-containing film. Er-related 1.54 μm emission has been investigated under several excitation conditions upon different kinds of Ge, Er codoped ZnO thin films. 1.54 μm PL enhancement accompanied by the appearance of nc-Ge implies a significant correlation between nc-Ge and PL emission of Er^(3+). The increased intensity of 1.54 μm in Ge:Er:ZnO film is considered to come from the joint effect of the local potential distortion around Er^(3+)and the possibleenergy transfer from nc-Ge to Er^(3+).展开更多
基金supported by the National Natural Science Foundation of China(62175071 and 32071399)the Guangdong Basic and Applied Basic Research Foundation(2021A1515011988 and 2021A1515110265)+2 种基金the Project of the Education Department of Guangdong Province,China(2021KTSCX238 and 2022ZDZX2077)the Project of Guangdong Food and Drug Vocational College(2020ZR01,2022ZR02 and 2024GCZX01)the Open Foundation of Key Laboratory of Optoelectronic Science and Technology for Medicine(Fujian Normal University),Ministry of Education,China(JYG2008 and JYG2009).
文摘Programmed cell death(PCD)plays a crucial role in the biological processes of living organisms and occurs in various forms,such as apoptosis,necroptosis and ferroptosis.However,traditional methods for PCD analysis are time-consuming and complex.In this paper,we propose a facile surface-enhanced Raman spectroscopy(SERS)-based strategy for the real-time analysis of three PCD patterns utilizing black phosphorus–gold nanoparticles(BP–Au NPs)as the ultrasensitive unlabeled Raman probe.BP–Au NPs,which possess excellent biocompatibility,are capable of detecting dye molecules at concentrations as low as 5×10^(-8)M and remain stable for at least one week in different physiological environments.In view of this,BP–Au NPs-based SERS technique can distinguish the tiny differences in the molecular fingerprints of cancer cells undergoing three PCD patterns(apoptosis,necroptosis and ferroptosis)triggered by doxorubicin,shikonin and erastin,respectively.We also have real-time monitoring of the intracellular molecular events during PCD,which spy the fluctuations of some typical SERS bands assigned to protein,DNA and lipid,revealing the unique phenotypic characteristics of each PCD pattern.This strategy provides a detailed and comprehensive analysis of the mechanisms of drug-induced PCD at the Raman level.
基金atural Science Foundation of Guangdong Province(2020A1515010541)Science and Technology Project of Shenzhen(JCYJ20180305124930169,JCYJ20190808143419622,ZDSYS201707271014468)。
文摘In this paper, a controllable one-step doping method has been successfully adopted in the cesium copper iodide perovskite’s luminescence, a high-quality white-light emission with Commission Internationale de l’Eclairage(CIE) coordinates of(0.3397, 0.3325), and a color rendering index(CRI) reaching up to 90 was realized in a convenient way. Through adding impurities into the Cs_(3)Cu_(2)I_(5) system, high efficiency and stable CsCu_(2)I_(3) was synthesized, and the coexistence of varied high luminescence phases realized the white lighting.Strikingly, blue-emitting Cs_(3)Cu_(2)I_(5) and yellow-emitting CsCu_(2)I_(3) could coexist, and their respective luminescence was not interacted in the compound, which was beneficial for acquiring a single emission and highly efficient white lighting. This work carried out a deep exploration of the Cu-based metal halides, and would be favorable to the applications of lead-free perovskites.
基金National Natural Science Foundation of China(NSFC)(11475105)
文摘ZnO films containing Er and Ge nanocrystals(nc-Ge) were synthesized and their photoluminescence(PL) properties were studied. Visible and near-infrared PL intensities are found to be greatly increased in nc-Ge-containing film. Er-related 1.54 μm emission has been investigated under several excitation conditions upon different kinds of Ge, Er codoped ZnO thin films. 1.54 μm PL enhancement accompanied by the appearance of nc-Ge implies a significant correlation between nc-Ge and PL emission of Er^(3+). The increased intensity of 1.54 μm in Ge:Er:ZnO film is considered to come from the joint effect of the local potential distortion around Er^(3+)and the possibleenergy transfer from nc-Ge to Er^(3+).
