AIMTo understand the interference of carbohydrates absorbance in nucleic acids signals during diagnosis of malignancy using Fourier transform infrared (FTIR) spectroscopy.METHODSWe used formalin fixed paraffin embedde...AIMTo understand the interference of carbohydrates absorbance in nucleic acids signals during diagnosis of malignancy using Fourier transform infrared (FTIR) spectroscopy.METHODSWe used formalin fixed paraffin embedded colonic tissues to obtain infrared (IR) spectra in the mid IR region using a bruker II IR microscope with a facility for varying the measurement area by varying the aperture available. Following this procedure we could measure different regions of the crypt circles containing different biochemicals. Crypts from 18 patients were measured. Circular crypts with a maximum diameter of 120 μm and a lumen of about 30 μm were selected for uniformity. The spectral data was analyzed using conventional and advanced computational methods.RESULTSAmong the various components that are observed to contribute to the diagnostic capabilities of FTIR, the carbohydrates and nucleic acids are prominent. However there are intrinsic difficulties in the diagnostic capabilities due to the overlap of major absorbance bands of nucleic acids, carbohydrates and phospholipids in the mid-IR region. The result demonstrates colonic tissues as a biological system suitable for studying interference of carbohydrates and nucleic acids under ex vivo conditions. Among the diagnostic parameters that are affected by the absorbance from nucleic acids is the RNA/DNA ratio, dependent on absorbance at 1121 cm<sup>-1</sup> and 1020 cm<sup>-1</sup> that is used to classify the normal and cancerous tissues especially during FTIR based diagnosis of colonic malignancies. The signals of the nucleic acids and the ratio (RNA/DNA) are likely increased due to disappearance of interfering components like carbohydrates and phosphates along with an increase in amount of RNA.CONCLUSIONThe present work, proposes one mechanism for the observed changes in the nucleic acid absorbance in mid-IR during disease progression (carcinogenesis).展开更多
Purpose:Fingertip injuries are common in industrial production activities as well as in domestic work.Loss of pulp hampers daily life activities.Functional and aesthetic aspects are important in fingertip reconstructi...Purpose:Fingertip injuries are common in industrial production activities as well as in domestic work.Loss of pulp hampers daily life activities.Functional and aesthetic aspects are important in fingertip reconstruction.The bone is usually exposed along with soft tissue loss.Therefore to reconstruct the pulp flap with adequate bulk is required.Methods:We reported a case series of 12 patients with the injury over the volar aspect of distal phalanx of the index or middle finger.In all cases,laterally based thenar flap was chosen.The flap donor site was closed primarily in most of cases,while 4 patients required skin graft.The flap was detached between 2e3 weeks.Functional assessment was done using static and dynamic 2-point discrimination and range of motion at each joint.The aesthetic outcome was assessed through questionnaire.The results were analyzed using the unpaired t-test(SPSS version 21).Results:Partial necrosis occurred in 2 cases while rest of flaps survived successfully.Static 2-point discrimination ranged from 6e10 mm,mean 8.6 mm;and dynamic 2-point discrimination ranged from 8e10 mm,mean 8.9 mm.The mean satisfaction score was(4.0±0.55).Conclusion:Thenar flap is a good choice for reconstruction of the finger pulp as it provides the bulk with good functional and aesthetic outcome.展开更多
文摘AIMTo understand the interference of carbohydrates absorbance in nucleic acids signals during diagnosis of malignancy using Fourier transform infrared (FTIR) spectroscopy.METHODSWe used formalin fixed paraffin embedded colonic tissues to obtain infrared (IR) spectra in the mid IR region using a bruker II IR microscope with a facility for varying the measurement area by varying the aperture available. Following this procedure we could measure different regions of the crypt circles containing different biochemicals. Crypts from 18 patients were measured. Circular crypts with a maximum diameter of 120 μm and a lumen of about 30 μm were selected for uniformity. The spectral data was analyzed using conventional and advanced computational methods.RESULTSAmong the various components that are observed to contribute to the diagnostic capabilities of FTIR, the carbohydrates and nucleic acids are prominent. However there are intrinsic difficulties in the diagnostic capabilities due to the overlap of major absorbance bands of nucleic acids, carbohydrates and phospholipids in the mid-IR region. The result demonstrates colonic tissues as a biological system suitable for studying interference of carbohydrates and nucleic acids under ex vivo conditions. Among the diagnostic parameters that are affected by the absorbance from nucleic acids is the RNA/DNA ratio, dependent on absorbance at 1121 cm<sup>-1</sup> and 1020 cm<sup>-1</sup> that is used to classify the normal and cancerous tissues especially during FTIR based diagnosis of colonic malignancies. The signals of the nucleic acids and the ratio (RNA/DNA) are likely increased due to disappearance of interfering components like carbohydrates and phosphates along with an increase in amount of RNA.CONCLUSIONThe present work, proposes one mechanism for the observed changes in the nucleic acid absorbance in mid-IR during disease progression (carcinogenesis).
文摘Purpose:Fingertip injuries are common in industrial production activities as well as in domestic work.Loss of pulp hampers daily life activities.Functional and aesthetic aspects are important in fingertip reconstruction.The bone is usually exposed along with soft tissue loss.Therefore to reconstruct the pulp flap with adequate bulk is required.Methods:We reported a case series of 12 patients with the injury over the volar aspect of distal phalanx of the index or middle finger.In all cases,laterally based thenar flap was chosen.The flap donor site was closed primarily in most of cases,while 4 patients required skin graft.The flap was detached between 2e3 weeks.Functional assessment was done using static and dynamic 2-point discrimination and range of motion at each joint.The aesthetic outcome was assessed through questionnaire.The results were analyzed using the unpaired t-test(SPSS version 21).Results:Partial necrosis occurred in 2 cases while rest of flaps survived successfully.Static 2-point discrimination ranged from 6e10 mm,mean 8.6 mm;and dynamic 2-point discrimination ranged from 8e10 mm,mean 8.9 mm.The mean satisfaction score was(4.0±0.55).Conclusion:Thenar flap is a good choice for reconstruction of the finger pulp as it provides the bulk with good functional and aesthetic outcome.
