We demonstrate low-frequency interferometric impulsive stimulated Raman scattering(ISRS)imaging with high robustness to distortions by optical scattering.ISRS is a pump−probe coherent Raman spectroscopy that can captu...We demonstrate low-frequency interferometric impulsive stimulated Raman scattering(ISRS)imaging with high robustness to distortions by optical scattering.ISRS is a pump−probe coherent Raman spectroscopy that can capture Raman vibrational spectra.Recording of ISRS spectra requires isolation of a probe pulse from the pump pulse.While this separation is simple in nonscattering specimens,such as liquids,scattering leads to significant pump pulse contamination and prevents the extraction of a Raman spectrum.We introduce a robust method for ISRS microscopy that works in complex scattering samples.High signal-to-noise ISRS spectra are obtained even when the pump and probe pulses pass through many scattering layers.展开更多
基金grateful for funding from DE-FOA-0002603(R.A.B.)Alicia Williams from the Morgridge Institute provided scientific editing supportsupport from ANR-21-ESRS-0002 IDEC,EU ICT 101016923 CRIMSON,EU EIC 101099058 VIRUSONG,and EU ERC 101052911(H.R.)and NIH 5 R21GM135772-02(J.W.W.and R.A.B.).
文摘We demonstrate low-frequency interferometric impulsive stimulated Raman scattering(ISRS)imaging with high robustness to distortions by optical scattering.ISRS is a pump−probe coherent Raman spectroscopy that can capture Raman vibrational spectra.Recording of ISRS spectra requires isolation of a probe pulse from the pump pulse.While this separation is simple in nonscattering specimens,such as liquids,scattering leads to significant pump pulse contamination and prevents the extraction of a Raman spectrum.We introduce a robust method for ISRS microscopy that works in complex scattering samples.High signal-to-noise ISRS spectra are obtained even when the pump and probe pulses pass through many scattering layers.
