BACKGROUND Numerous studies have found that patients experiencing sudden sensorineural hearing loss(SSHL),with or without accompanying vertigo,often show impaired vestibular function.However,there is a dearth of studi...BACKGROUND Numerous studies have found that patients experiencing sudden sensorineural hearing loss(SSHL),with or without accompanying vertigo,often show impaired vestibular function.However,there is a dearth of studies analyzing vestibularevoked myogenic potentials(VEMPs)in SSHL patients across various age groups.AIM To investigate vestibular condition in SSHL patients across various age demographics.METHODS Clinical data of 84 SSHL patients were investigated retrospectively.Audiometry,cervical vestibular evoked myogenic potentials(c-VEMPs),and ocular vestibular evoked myogenic potentials(o-VEMPs)were conducted on these patients.Parameters assessed included the latencies of P1 and N1 waves,as well as the amplitudes of P1–N1 waves.Moreover,the study evaluated the influence of factors such as sex,affected side,configuration of hearing loss,and presence of accompanying vertigo.RESULTS Among the 84 SSHL patients,no significant differences were observed among the three groups in terms of gender,affected side,and the presence or absence of vertigo.Group II(aged 41–60 years)had the highest number of SSHL cases.The rates of absent o-VEMPs in the affected ears were 20.83%,31.58%,and 22.72%for the three age groups,respectively,with no statistically significant difference among them.The rates of absent c-VEMPs in the affected ears were 8.3%,34.21%,and 18.18%for the three age groups,respectively,with significant differences.In the unaffected ears,there were differences observed in the extraction rates of o-VEMPs in the unaffected ears among the age groups.In the three age groups,no significant differences were noted in the three age groups in the latencies of P1 and N1 waves or in the amplitude of N1–P1 waves for c-VEMPs and o-VEMPs,either on the affected side or on the unaffected side,across the three age groups.CONCLUSION The extraction rate of VEMPs is more valuable than parameters.Regardless of the presence of vertigo,vestibular organs are involved in SSHL.Notably,SSHL patients aged 41–60 appear more susceptible to damage to the inferior vestibular nerve and saccule.展开更多
Intramuscular fat （IMF） is a crucial factor in the quality of chicken meat. The genetic basis underlying it is complex. Follicle-stimulating hormone （FSH）, well-known as an effector in reproductive tissues, was re...Intramuscular fat （IMF） is a crucial factor in the quality of chicken meat. The genetic basis underlying it is complex. Follicle-stimulating hormone （FSH）, well-known as an effector in reproductive tissues, was recently discovered to stimulate abdominal fat accumulation in chicken. The effect of FSH on IMF accumulation and the underlying molecular regulatory mechanisms controlling both IMF and abdominal fat deposition in vivo are largely unknown. In this study, two groups of chickens were treated with chicken FSH or a placebo. The lipid content of breast muscle, abdominal fat volume, and serum concentrations of FSH were examined. Related genes implicated in breast muscle and abdominal fat accumulation were also investigated. Compared to the control group, the triglyceride （TG） content of breast muscle and the percentage of abdominal fat in FSH-treated chickens were significantly increased by 64.9% and 56.5% （P〈0.01）, respectively. The FSH content in the serum of FSH-treated chickens was 2.1 times than that of control chickens （P〈0.01）. Results from quantitative real-time polymerase chain reaction （qRT-PCR） assays showed that relative expression levels of fatty acid synthase （FAS）, lipoprotein lipase （LPL）, diacylglycerol acyltransferase 2 （DGAT2）, adipocyte fatty acid binding protein （A-FABP）, and peroxisome proliferator-activated receptor ~ （PPARy） were significantly upregulated in breast muscle following FSH treatment （P〈0.01）. Treatment with FSH also signifi- cantly increased relative expression levels of FAS, LPL, DGA T2, A-FABP, and PPARy in abdominal fat tissue （P〈0.05） The results of principal component analysis （PCA） for gene expression （breast muscle and abdominal fat） showed that the control and FSH treatment groups were well separated, which indicated the reliability of the data. This study demonstrates that FSH plays an important role in IMF accumulation in female chickens, which likely involves the regulation of biosynthesis genes related to lipid metabolism.展开更多
Objective To explore the potential function of nutrition on kisspeptin/kisspeptin receptor （kisslr） system in the arcuate nucleus （ARC） of male rats. Methods Pregnant rats were obtained and male pups were used to ...Objective To explore the potential function of nutrition on kisspeptin/kisspeptin receptor （kisslr） system in the arcuate nucleus （ARC） of male rats. Methods Pregnant rats were obtained and male pups were used to establish obesity model. Body parameters and blood samples were evaluated. Immunohistochemistry was used to determine the localizations and protein levels of kisspeptin, kisslr, and leptin receptor （LepR） immunoreactivity （IR） in ARC. QRT-PCR was used to determine kissl-, kisslr-, LepR-, and GnRH-mRNA levels. Results The establishment of obesity model was successful as body parameters and hormones levels changed noticeably. Kisspeptin-, kisslr-, and LepR-IR were detected and protein levels decreased significantly in high-fat-diets （HFD） rats than controls. The mRNA levels of kissl, LepR and GnRH significantly decreased in the ARC of HFD-fed rats. No difference was observed in kisslr mRNA levels between the two groups. Conclusion These data suggest that failure to increase GnRH levels with HFD may be associated with pubertal down-regulation of LepR and kisspeptin/kisslr system in the ARC of male rats.展开更多
Objective:To explore the mechanism of electroacupuncture(EA)on inhibiting tumor growth in breast cancer-bearing mice.Methods:Thirty Bagg Albino(c)(BALB/c)mice were randomly divided into a sham-operation group,a tumor ...Objective:To explore the mechanism of electroacupuncture(EA)on inhibiting tumor growth in breast cancer-bearing mice.Methods:Thirty Bagg Albino(c)(BALB/c)mice were randomly divided into a sham-operation group,a tumor group,a chemotherapy group,and an acupuncture group,and an EA group,with six mice in each one.Except in the sham-operation group,4T1 cells were injected into the fourth mammary fat pad on the right side of each mouse in the rest groups to develop breast-cancer bearing models.In the sham-operation group,the sham operation was underwent by injecting normal saline with the same volume as 4T1 cell suspension.After successful modeling,the intraperitoneal injection of doxorubicin solution was delivered in the chemotherapy group.In the acupuncture group,needles was inserted at“Zusanli”(ST36)and“Sanyinjiao”(SP6)perpendicularly,without manipulation,once daily and needles were re-tained for 15 min.In the EA group,on the basis of the operation as the acupuncture group,electric stimulation was attached to“ST36”(positive electrode)and“SP6”(negative electrode),once daily,15 min each time.Starting on Day 1 of intervention,the changes of tumor volume was observed once every two days in each group photographed.The sample was collected on Day 21 of intervention.The tumors were photographed and weighed.Hematoxylin-eosin(HE)staining was used to observe the pathologi-cal changes of tumor tissue.Western blot,fluorescence quantitative polymerase chain reaction(FQ-PCR),and immunohistochemistry(IHC)were adopted to detect the protein expression,mRNA expression,and positive expression of CYLD,BCL-3,and P50 in tumor tissue,respectively.Results:(1)The tumor volume in the tumor group,chemotherapy group,acupuncture group,and EA group was(815.67±275.61)mm^(3),(197.50±66.11)mm^(3),(412.62±58.96)mm^(3),and(327.51±125.46)mm^(3),respectively,and the tumor mass was(1.21±0.39)g,(0.39±0.05)g,(0.66±0.06)g,and(0.55±0.11)g on Day 21 of intervention,respectively.Compared with the tumor group,the tumor vol-ume and mass were lower in the chemotherapy group,acupuncture group,and EA group(P<0.05).(2)Respectively,in the tumor group,chemotherapy group,acupuncture group,and EA group,the mRNA expression of CYLD in tumor tissue was 1.01±0.19,2.90±0.24,2.55±0.03,and 4.01±0.32;the protein expression was 1.00±0.00,1.88±0.16,1.64±0.11,and 2.30±0.29;and the positive expres-sion was 0.06±0.01,0.11±0.01,0.11±0.004,and 0.14±0.003.Compared with the tumor group,the mRNA expression,protein expression,and positive expression of CYLD were higher in the inter-vention groups(P<0.05),and each expression in the EA group was higher in comparison with the chemotherapy and acupuncture groups(P<0.05).(3)The mRNA expression of BCL-3 in tumor tissue was 3.34±0.27,1.11±0.04,1.25±0.10,and 1.00±0.04;and the protein expression was 1.00±0.00,0.59±0.15,0.38±0.08,and 0.38±0.13;and the positive expression was 0.12±0.01,0.06±0.02,0.07±0.01,and 0.05±0.003 in the tumor group,chemotherapy group,acupuncture group,and EA group,respectively.Compared with the tumor group,the mRNA expression,protein expression,and pos-itive expression of BCL-3 were all lower in the intervention groups(P<0.05).(4)The mRNA expression of P50 in the tumor tissue was 3.69±0.17,1.69±0.07,1.74±0.05,and 1.00±0.06;and the pro-tein expression was 1.00±0.00,0.33±0.15,0.67±0.14,and 0.38±0.21;and the positive expression was 0.13±0.01,0.07±0.02,0.08±0.003,and 0.06±0.01 in the tumor group,chemotherapy group,acupuncture group,and EA group,respectively.Compared with the tumor group,the mRNA expression,protein expression,and positive expression of P50 in the tumor tissues of mice were lower in the inter-vention groups(P<0.05),and the mRNA expression in the EA group was lower when compared with the chemotherapy group and acupuncture group(P<0.05),while the protein expression of it in the chemotherapy group was lower than that in the acupuncture group(P<0.05).Conclusion:All of chemotherapy,acupuncture,and EA can inhibit tumor growth,which may be related to the up-regulation of CYLD expression and the down-regulation of BCL-3 and P50 expression in breast can-cer tissue.EA obtains a superior effect for up-regulating CYLD expression in comparison with chemother-apy and acupuncture.展开更多
基金the Innovative Program of Hebei Provincial Eye Hospital,No.2023ZZ107.
文摘BACKGROUND Numerous studies have found that patients experiencing sudden sensorineural hearing loss(SSHL),with or without accompanying vertigo,often show impaired vestibular function.However,there is a dearth of studies analyzing vestibularevoked myogenic potentials(VEMPs)in SSHL patients across various age groups.AIM To investigate vestibular condition in SSHL patients across various age demographics.METHODS Clinical data of 84 SSHL patients were investigated retrospectively.Audiometry,cervical vestibular evoked myogenic potentials(c-VEMPs),and ocular vestibular evoked myogenic potentials(o-VEMPs)were conducted on these patients.Parameters assessed included the latencies of P1 and N1 waves,as well as the amplitudes of P1–N1 waves.Moreover,the study evaluated the influence of factors such as sex,affected side,configuration of hearing loss,and presence of accompanying vertigo.RESULTS Among the 84 SSHL patients,no significant differences were observed among the three groups in terms of gender,affected side,and the presence or absence of vertigo.Group II(aged 41–60 years)had the highest number of SSHL cases.The rates of absent o-VEMPs in the affected ears were 20.83%,31.58%,and 22.72%for the three age groups,respectively,with no statistically significant difference among them.The rates of absent c-VEMPs in the affected ears were 8.3%,34.21%,and 18.18%for the three age groups,respectively,with significant differences.In the unaffected ears,there were differences observed in the extraction rates of o-VEMPs in the unaffected ears among the age groups.In the three age groups,no significant differences were noted in the three age groups in the latencies of P1 and N1 waves or in the amplitude of N1–P1 waves for c-VEMPs and o-VEMPs,either on the affected side or on the unaffected side,across the three age groups.CONCLUSION The extraction rate of VEMPs is more valuable than parameters.Regardless of the presence of vertigo,vestibular organs are involved in SSHL.Notably,SSHL patients aged 41–60 appear more susceptible to damage to the inferior vestibular nerve and saccule.
基金supported by the National Natural Science Foundation of China(No.31372305)the Agricultural Science and Technology Innovation Program(No.ASTIP-IAS04)the Project of State Key Laboratory of Animal Nutrition(No.2004DA125184G1101),China
文摘Intramuscular fat （IMF） is a crucial factor in the quality of chicken meat. The genetic basis underlying it is complex. Follicle-stimulating hormone （FSH）, well-known as an effector in reproductive tissues, was recently discovered to stimulate abdominal fat accumulation in chicken. The effect of FSH on IMF accumulation and the underlying molecular regulatory mechanisms controlling both IMF and abdominal fat deposition in vivo are largely unknown. In this study, two groups of chickens were treated with chicken FSH or a placebo. The lipid content of breast muscle, abdominal fat volume, and serum concentrations of FSH were examined. Related genes implicated in breast muscle and abdominal fat accumulation were also investigated. Compared to the control group, the triglyceride （TG） content of breast muscle and the percentage of abdominal fat in FSH-treated chickens were significantly increased by 64.9% and 56.5% （P〈0.01）, respectively. The FSH content in the serum of FSH-treated chickens was 2.1 times than that of control chickens （P〈0.01）. Results from quantitative real-time polymerase chain reaction （qRT-PCR） assays showed that relative expression levels of fatty acid synthase （FAS）, lipoprotein lipase （LPL）, diacylglycerol acyltransferase 2 （DGAT2）, adipocyte fatty acid binding protein （A-FABP）, and peroxisome proliferator-activated receptor ~ （PPARy） were significantly upregulated in breast muscle following FSH treatment （P〈0.01）. Treatment with FSH also signifi- cantly increased relative expression levels of FAS, LPL, DGA T2, A-FABP, and PPARy in abdominal fat tissue （P〈0.05） The results of principal component analysis （PCA） for gene expression （breast muscle and abdominal fat） showed that the control and FSH treatment groups were well separated, which indicated the reliability of the data. This study demonstrates that FSH plays an important role in IMF accumulation in female chickens, which likely involves the regulation of biosynthesis genes related to lipid metabolism.
基金supported by grants from the Natural Science Foundation of Shandong,Peoples’ Republic of China(ZR2012HM039)
文摘Objective To explore the potential function of nutrition on kisspeptin/kisspeptin receptor （kisslr） system in the arcuate nucleus （ARC） of male rats. Methods Pregnant rats were obtained and male pups were used to establish obesity model. Body parameters and blood samples were evaluated. Immunohistochemistry was used to determine the localizations and protein levels of kisspeptin, kisslr, and leptin receptor （LepR） immunoreactivity （IR） in ARC. QRT-PCR was used to determine kissl-, kisslr-, LepR-, and GnRH-mRNA levels. Results The establishment of obesity model was successful as body parameters and hormones levels changed noticeably. Kisspeptin-, kisslr-, and LepR-IR were detected and protein levels decreased significantly in high-fat-diets （HFD） rats than controls. The mRNA levels of kissl, LepR and GnRH significantly decreased in the ARC of HFD-fed rats. No difference was observed in kisslr mRNA levels between the two groups. Conclusion These data suggest that failure to increase GnRH levels with HFD may be associated with pubertal down-regulation of LepR and kisspeptin/kisslr system in the ARC of male rats.
基金Supported by National Natural Science Foundation of China General Program:No.81072883,No.81173342,No.81473773Hebei Provincial Natural Science Foundation:No.2023423029。
文摘Objective:To explore the mechanism of electroacupuncture(EA)on inhibiting tumor growth in breast cancer-bearing mice.Methods:Thirty Bagg Albino(c)(BALB/c)mice were randomly divided into a sham-operation group,a tumor group,a chemotherapy group,and an acupuncture group,and an EA group,with six mice in each one.Except in the sham-operation group,4T1 cells were injected into the fourth mammary fat pad on the right side of each mouse in the rest groups to develop breast-cancer bearing models.In the sham-operation group,the sham operation was underwent by injecting normal saline with the same volume as 4T1 cell suspension.After successful modeling,the intraperitoneal injection of doxorubicin solution was delivered in the chemotherapy group.In the acupuncture group,needles was inserted at“Zusanli”(ST36)and“Sanyinjiao”(SP6)perpendicularly,without manipulation,once daily and needles were re-tained for 15 min.In the EA group,on the basis of the operation as the acupuncture group,electric stimulation was attached to“ST36”(positive electrode)and“SP6”(negative electrode),once daily,15 min each time.Starting on Day 1 of intervention,the changes of tumor volume was observed once every two days in each group photographed.The sample was collected on Day 21 of intervention.The tumors were photographed and weighed.Hematoxylin-eosin(HE)staining was used to observe the pathologi-cal changes of tumor tissue.Western blot,fluorescence quantitative polymerase chain reaction(FQ-PCR),and immunohistochemistry(IHC)were adopted to detect the protein expression,mRNA expression,and positive expression of CYLD,BCL-3,and P50 in tumor tissue,respectively.Results:(1)The tumor volume in the tumor group,chemotherapy group,acupuncture group,and EA group was(815.67±275.61)mm^(3),(197.50±66.11)mm^(3),(412.62±58.96)mm^(3),and(327.51±125.46)mm^(3),respectively,and the tumor mass was(1.21±0.39)g,(0.39±0.05)g,(0.66±0.06)g,and(0.55±0.11)g on Day 21 of intervention,respectively.Compared with the tumor group,the tumor vol-ume and mass were lower in the chemotherapy group,acupuncture group,and EA group(P<0.05).(2)Respectively,in the tumor group,chemotherapy group,acupuncture group,and EA group,the mRNA expression of CYLD in tumor tissue was 1.01±0.19,2.90±0.24,2.55±0.03,and 4.01±0.32;the protein expression was 1.00±0.00,1.88±0.16,1.64±0.11,and 2.30±0.29;and the positive expres-sion was 0.06±0.01,0.11±0.01,0.11±0.004,and 0.14±0.003.Compared with the tumor group,the mRNA expression,protein expression,and positive expression of CYLD were higher in the inter-vention groups(P<0.05),and each expression in the EA group was higher in comparison with the chemotherapy and acupuncture groups(P<0.05).(3)The mRNA expression of BCL-3 in tumor tissue was 3.34±0.27,1.11±0.04,1.25±0.10,and 1.00±0.04;and the protein expression was 1.00±0.00,0.59±0.15,0.38±0.08,and 0.38±0.13;and the positive expression was 0.12±0.01,0.06±0.02,0.07±0.01,and 0.05±0.003 in the tumor group,chemotherapy group,acupuncture group,and EA group,respectively.Compared with the tumor group,the mRNA expression,protein expression,and pos-itive expression of BCL-3 were all lower in the intervention groups(P<0.05).(4)The mRNA expression of P50 in the tumor tissue was 3.69±0.17,1.69±0.07,1.74±0.05,and 1.00±0.06;and the pro-tein expression was 1.00±0.00,0.33±0.15,0.67±0.14,and 0.38±0.21;and the positive expression was 0.13±0.01,0.07±0.02,0.08±0.003,and 0.06±0.01 in the tumor group,chemotherapy group,acupuncture group,and EA group,respectively.Compared with the tumor group,the mRNA expression,protein expression,and positive expression of P50 in the tumor tissues of mice were lower in the inter-vention groups(P<0.05),and the mRNA expression in the EA group was lower when compared with the chemotherapy group and acupuncture group(P<0.05),while the protein expression of it in the chemotherapy group was lower than that in the acupuncture group(P<0.05).Conclusion:All of chemotherapy,acupuncture,and EA can inhibit tumor growth,which may be related to the up-regulation of CYLD expression and the down-regulation of BCL-3 and P50 expression in breast can-cer tissue.EA obtains a superior effect for up-regulating CYLD expression in comparison with chemother-apy and acupuncture.
