BACKGROUND Pharmacological treatments are commonly used in individuals experiencing perinatal depression(PPD);however,a debate regarding the reproductive safety of antidepressants is ongoing.Many pregnant women opt to...BACKGROUND Pharmacological treatments are commonly used in individuals experiencing perinatal depression(PPD);however,a debate regarding the reproductive safety of antidepressants is ongoing.Many pregnant women opt to discontinue antidepressant out of concern about potential negative effects on the developing fetus,while slow and ineffective antidepressant medications hinder improved outcomes in women with PPD.In recent years,bright light therapy(BLT)has gained traction as a treatment option for PPD;however,clinical trials findings examining the efficacy of BLT in this population have been inconclusive.AIM To validate the feasibility and safety of BLT for the treatment of PPD.METHODS We performed a meta-analysis of randomized controlled trials of patients with PPD treated with BLT vs placebo following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis.We searched PubMed,Embase,the Cochrane Library,and Web of Science for randomized controlled studies published up to December 2023.The results were evaluated using the standardized mean difference of improvement for depression scores and odds ratios(ORs)for remission rate,response rate,incidence of adverse events,and dropout rate.RESULTS The BLT group had higher PPD response rate[50.68%vs 33.08%;OR=2.05;95% confidence interval(CI):1.25-3.35;P=0.004;I^(2)=35%]and remission rate(54.10%vs 18.52%;OR=5.00;95%CI:2.09-11.99;P=0.0003;I^(2)=0%)than the placebo group.Improvements in depression scores were higher in the BLT group than the placebo group for the overall efficacy(standardized mean difference=-0.47;95%CI:-0.80 to-0.13;P=0.007).No significant differences between the two groups in drop-outs(21.84%vs 29.63%;OR=0.63;95%CI:0.31-1.29;P=0.21;I^(2)=0%)or adverse events(17.89%vs 9.68%;OR=2.01;95%CI:0.95-4.25;P=0.07;I^(2)=0%)were observed.CONCLUSION BLT can potentially treat PPD,showing better results than the control group in this study.BLT is effective and safe and could increase the available therapeutic options for PPD.展开更多
Waterlogging stress is one of the greatest environmental threats to kiwifruit growth and development.ERF-VII proteins have been demonstrated to play pivotal roles in regulating plant tolerance to waterlogging.Neverthe...Waterlogging stress is one of the greatest environmental threats to kiwifruit growth and development.ERF-VII proteins have been demonstrated to play pivotal roles in regulating plant tolerance to waterlogging.Nevertheless,the genome-wide role of ERF-VII in kiwifruit waterlogging stress tolerance remains unclear.Here,we report the function and regulatory network of an ERF-VII transcription factor located to the nucleus,Av ERF73,in kiwifruit waterlogging tolerance.Overexpression of Av ERF73 in Arabidopsis thaliana and A.chinensis cv.Hongyang enhanced waterlogging tolerance in transgenic plants.Furthermore,we performed transcriptome analysis(RNA-seq)and DNA affinity purification sequencing(DAP-seq)to explore the regulatory mechanism of Av ERF73.RNA-seq coupled with DAP-seq showed that Av ERF73 might directly activate Ac NAC022 involved in the“cellular response to hypoxia”process and Ac HMGS1 involved in the mevalonate pathway to respond to waterlogging,which were also confirmed by a dual-luciferase reporter assay.Based on our results,we propose a putative working model for controlling waterlogging tolerance by Av ERF73 in kiwifruit.展开更多
Lycium barb arum,known as wolfberry or goji berry,is consumed by humans as a medicine and a food homology product.Conventionally grown wolfberry is often treated extensively with pesticides,which could pose a hazard t...Lycium barb arum,known as wolfberry or goji berry,is consumed by humans as a medicine and a food homology product.Conventionally grown wolfberry is often treated extensively with pesticides,which could pose a hazard to humans.Here,the degradation dynamics of dinotefuran and its 2 metabolites(1-methyl-3-(tetrahydro-3-furylmethyl)urea(UF)and 1-methyl-3-(tetrahydro-3-furylmethyl)guanidine(DN)),during wolfberry cultivation and processing was investigated.The half-life(T_(1/2))of dinotefuran was 11.36 and 9.76 days,respectively,under the recommended dosage and double the recommended dosage.During the oven and sun drying processes,processing factors(PFs)of dinotefuran were 1.07-1.34,implying the enrichment of pesticide residues.Decoction process made the removal rate of dinotefuran reach 87.48%,which is higher than that of the brewing process(14.7%),while dinotefuran remained in the wine with high ethanol content in the alcohol soaking process.The hazard quotient(HQ)of dinotefuran,as determined via dietary risk assessment combined with PFs,was<1,indicating an acceptable risk for human consumption.Bioaccessibility of dinotefuran in the three digestive stages were intestinal(18.20%-88.08%)>gastric(5.45%-86.72%)>oral(23.18%)via in vitro simulated digestive system.These findings provide scientific evidence for reasonable application and risk assessment of dinotefuran residues in wolfberry.展开更多
Dehydrin(DHN)enhances plant resistance to environmental stress by regulating the synthesis of osmotic adjustment substances and scavenging reactive oxygen species.However,the role of PbDHN3 under salt stress remains u...Dehydrin(DHN)enhances plant resistance to environmental stress by regulating the synthesis of osmotic adjustment substances and scavenging reactive oxygen species.However,the role of PbDHN3 under salt stress remains unclear.In this study,salt stress induced high expression of PbDHN3,and the overexpression of PbDHN3(OE-PbDHN3)enhanced plant growth under salt stress compared to wild-type(WT)plants.OE-PbDHN3 plants exhibited higher chlorophyll content and root growth capacity than WT plants under salt stress.Transcriptome analysis revealed that PbDHN3 expression is associated with ethylene signaling pathways.OE-PbDHN3 transgenic plants substantially influenced ethylene content and the expression of related genes.Following treatment with exogenous ethephon,the transgenic lines notably inhibited the processes of ethylene synthesis and signaling transduction.OE-PbDHN3 transgenic lines treated with exogenous ethylene and the ethylene inhibitor 1-MCP demonstrated significant inhibition of ethylene synthesis and signaling transduction,while promoting root development and chlorophyll content.Under salt stress,OE-PbDHN3 downregulated the expression of ethylene biosynthesis genes PbACO1-like and PbACO2,and signal transduction genes PbEIN3-like during the initial stress phase.This early regulation mitigated the adverse effects of salt stress on the plants.These findings demonstrate that PbDHN3 ameliorates the ethylene-mediated plant growth phenotype under salt stress through regulation of ethylene synthesis and signal transduction.展开更多
The tobacco whitefly,Bemisia tabaci,is a notorious pest affecting various crops globally,and it exhibits high levels of resistance to various insecticides.Afidopyropen is a recently commercialized pyropene insecticide...The tobacco whitefly,Bemisia tabaci,is a notorious pest affecting various crops globally,and it exhibits high levels of resistance to various insecticides.Afidopyropen is a recently commercialized pyropene insecticide for B.tabaci control with high selectivity and a novel mode of action.We previously identified a high level of afidopyropen resistance in a field-collected population after selection in the lab,and named it the HD-Afi strain.In the present study,minimal cross-resistance in the HD-Afi strain was found between afidopyropen and other common chemical agents.However,the P450 enzyme activity in HD-Afi was 2.18 times the level in susceptible strain HD-S.Expression analysis revealed that two of 12 candidate P450 genes,namely CYP6DW3 and CYP4C64,were significantly up-regulated in HD-Afi.Silencing CYP6DW3 and CYP4C64 by RNA interference(RNAi)substantially increased the susceptibility of whitefly adults,confirming their involvement in afidopyropen resistance.Homology modeling and molecular docking analyses demonstrated stable binding of afidopyropen to CYP6DW3 and CYP4C64,with binding free energies of–6.87 and–6.11 kcal mol^(-1),respectively.The findings of this study suggest that the induction of CYP6DW3 and CYP4C64 facilitates afidopyropen detoxification,contributing to the development of resistance in B.tabaci.展开更多
Background: Intravoxel incoherent motion diffusion weighted imaging (IVIM-DWI) can not only observe the diffusion of tissue water molecules but also reflect the blood perfusion information of tissue microcirculation. ...Background: Intravoxel incoherent motion diffusion weighted imaging (IVIM-DWI) can not only observe the diffusion of tissue water molecules but also reflect the blood perfusion information of tissue microcirculation. IVIM-DWI has been applied in many clinical areas. However, few studies have addressed the use of IVIM-DWI for the evaluation of transarterial chemoembolization (TACE) response in hepatocellular carcinoma (HCC) patients. Objectives: The purpose of the present study was to explore the role of IVIM-DWI for the therapeutic response of TACE for HCC. Materials and Methods: Twenty patients underwent IVIM-DWI scan on a 3.0T magnetic resonance imaging instrument 1 - 3 days before and 30 to 40 days after TACE. The values of IVIM-DWI parameters, including standard apparent diffusion coefficient (ADC), pure diffusion coefficient (Dslow), pseudo-diffusion coefficient (Dfast) and perfusion fraction (f) were measured. The values of IVIM-DWI parameters before and after TACE were compared using paired t tests. The values between responsive and non-responsive groups were compared using independent-samples t test. P 0.05 indicated statistical significance. Results: After TACE, the ADC and Dslow values in the tumors increased significantly, and the values of Dfast decreased significantly, while the values of f value did not change obviously. The ADC values in responsive group were remarkably higher than those in non-responsive group, the Dfast values in responsive group were significantly lower than those in non-responsive group, but the values of Dslow and f between the two groups were not different significantly. Conclusions: IVIM-DWI parameters can be used as potential markers for the therapeutic response of TACE for HCC.展开更多
In this paper,the bounded traveling wave solutions are examined for a system of partial differential equations with certain boundary conditions.Interestingly,this system can be used to describe the axially symmetric m...In this paper,the bounded traveling wave solutions are examined for a system of partial differential equations with certain boundary conditions.Interestingly,this system can be used to describe the axially symmetric motion of a semi-infinite incompressible hyperelastic cylindrical rod.With the aid of traveling wave transformations,the partial differential equations can be reduced to a traveling wave equation.The implicit analytical expressions determining the traveling waves are derived.Significantly,the influences of material parameters on the qualitative properties are discussed in detail by using the phase portraits of the traveling wave system. Smooth solutions such as the periodic traveling wave solutions and the solitary wave solutions with the peak form are shown.In particular,some interesting singular traveling wave solutions including the solitary cusp wave solutions and the periodic cusp wave solutions with the peak form are obtained.Numerical examples for all these waves are given.展开更多
In this paper,a nonlinear wave equation with variable coefficients is studied,interestingly,this equation can be used to describe the travelling waves propagating along the circular rod composed of a general compressi...In this paper,a nonlinear wave equation with variable coefficients is studied,interestingly,this equation can be used to describe the travelling waves propagating along the circular rod composed of a general compressible hyperelastic material with variable cross-sections and variable material densities.With the aid of Lou’s direct method1,the nonlinear wave equation with variable coefficients is reduced and two sets of symmetry transformations and exact solutions of the nonlinear wave equation are obtained.The corresponding numerical examples of exact solutions are presented by using different coefficients.Particularly,while the variable coefficients are taken as some special constants,the nonlinear wave equation with variable coefficients reduces to the one with constant coefficients,which can be used to describe the propagation of the travelling waves in general cylindrical rods composed of generally hyperelastic materials.Using the same method to solve the nonlinear wave equation,the validity and rationality of this method are verified.展开更多
1.Objective The study area is located at the southern Zhangguangcai range,which is thought to be the eastern part of the Central Asian Orogenic Belt(CAOB).The study area is closed to the eastern margin of the Songliao...1.Objective The study area is located at the southern Zhangguangcai range,which is thought to be the eastern part of the Central Asian Orogenic Belt(CAOB).The study area is closed to the eastern margin of the Songliao basin(Fig.1a).No petroleum exploration or related research work have been conducted in this area by far.Based on our most recent geological mapping,numerous hydrocarbon source rocks were firstly discovered within the Xinxing Formation,which is widely exposed from the central Jilin Province to the southeastern Heilongjiang province.The Xinxing Formation covers an area of nearly 1000 km2 with a total thickness of more than 1481 m(Regional Geology of Heilongjiang Province,1993).Therefore,the Xinxing Formation has huge potential to form oil and gas resources.This new discovery of hydrocarbon source rocks within the Xinxing Formation could provide critical clues for oil and gas resources’investigation in Northeast China,also may has important economic significance.展开更多
Skin aging is an increasingly prominent topic in the context of healthy aging.During the aging process,the skin’s barrier function diminishes,its water content decreases,wrinkles begin to form,and changes occur in th...Skin aging is an increasingly prominent topic in the context of healthy aging.During the aging process,the skin’s barrier function diminishes,its water content decreases,wrinkles begin to form,and changes occur in the gut microbiota composition.However,the relationship between gut microbiota and skin aging remains unclear.In this study,we explored skin rejuvenation in aged mice through fecal microbiota trans-plantation(FMT)using feces from young mice.The results demonstrated enhanced water retention,thick-ened stratum corneum,increased collagen content,and improved epithelial cell differentiation in aged mice following FMT.Notably,FMT particularly increased the abundance of Lactobacillus and Lactococcus in aged mice,which were nearly undetectable in untreated aged mice.Non-targeted and targeted meta-bolomics analyses indicated that FMT significantly elevated levels of tryptophan(Trp)and its microbiota metabolites(e.g.,indole-3-lactic acid(ILA))in the feces and serum of aged mice.Both Trp and ILA appeared to rejuvenate aged skin by activating the aryl hydrocarbon receptor(AhR)to promote epidermal cell dif-ferentiation.In conclusion,FMT from young mice rejuvenated aged skin via Trp-metabolizing bacteria(Lactobacillus and Lactococcus)and Trp-derived metabolites,suggesting that interventions targeting Trp metabolites may effectively improve skin aging.展开更多
Well-ordered hierarchically mesoporous/microporous carbon materials have been successfully fabricated by using dual soft-templating approach through compressed CO_(2).Pluronic F127 and different type of surfactants,in...Well-ordered hierarchically mesoporous/microporous carbon materials have been successfully fabricated by using dual soft-templating approach through compressed CO_(2).Pluronic F127 and different type of surfactants,including nonionic,cationic,and anionic surfactants,were used as dual templates to investigate the influence on the morphology and nanostructure of the as-prepared carbon samples.TEM,SEM,N2 sorption,wide-angle and small-angle XRD analysis were employed to reveal the well-ordered hierarchically micro-mesoporous structure with2 D hexagonal symmetry by using compressed CO_(2).The prepared HPC samples with different pressures as the catalyst carriers have been functioned by chlorosulfonic acid for the fructose conversion into HMF.Chlorosulfonic acid concentration,catalyst dosage and reaction temperature have been optimized for fructose-to-HMF transformation with the obtained catalyst.The performances of as-made HPC-SO_(3) H samples in HMF yield and reaction rate of fructose-to-HMF transformation have been investigated.The stability of the samples was also conducted in the dehydration of fructose to HMF for five cycles.The possible catalytic mechanism by using hierarchically porous carbon materials as catalyst support for fructose-to-HMF transformation was proposed.展开更多
Nanostructured zeolitic imidazolate frameworks(ZIF-8) was incorporated into the mixture of poly(ethylene glycol) methyl ether acrylate(PEGMEA) and pentaerythritol triacrylate(PETA) to synthesize mixed matrix membranes...Nanostructured zeolitic imidazolate frameworks(ZIF-8) was incorporated into the mixture of poly(ethylene glycol) methyl ether acrylate(PEGMEA) and pentaerythritol triacrylate(PETA) to synthesize mixed matrix membranes(MMMs) by in situ polymerization for CO_2/CH_4 separation. The solvent-free polymerization between PEGMEA and PETA was induced by UV light with 1-hydroxylcyclohexyl phenyl ketone as initiator. The chemical structural characterization was performed by Fourier transform infrared spectroscopy. The morphology was characterized by scanning electron microscope. The average chain-to-chain distance of the polymer chains in MMMs was investigated by X-ray diffraction. The thermal property was evaluated by differential scanning calorimetry. The CH_4 and CO_2 gas transport properties of MMMs are reported. The relationship between gas permeation–separation performances or physical properties and ZIF-8 loading is also discussed. However, the permeation–separation performance was not improved in Robeson upper bound plot compared with original polymer membrane as predicted. The significant partial pore blockage and polymer rigidification effect around the ZIFs confirmed by the increase in glass temperature and the decrease in the d-spacing, were mainly responsible for the failure in performance improvement, which offset the high diffusion induced by porous ZIF-8.展开更多
Objective:To study the transmembrane transport of chemical components of Chinese herbs and to explore the function of platycodin D (PD) on biomembranes.Methods:Interaction between PD and the dipalmitoylphosphatidylcho...Objective:To study the transmembrane transport of chemical components of Chinese herbs and to explore the function of platycodin D (PD) on biomembranes.Methods:Interaction between PD and the dipalmitoylphosphatidylcholine (DPPC) bilayer was reproduced by molecular dynamics simulation with the Martini force field.A model validation and methodological study were first performed,and were based on simulation investigations of transmembrane transport for three herbal compounds with distinct hydrophilic properties.Results:PD increased the mobility of the DPPC bilayer since its aglycone strongly interacted with the hydrophobic layer,which broke the structure of the gate layer,and weakened the ordered performance of hydrophobic tails.Conclusion:The Martini force field was successfully applied to the study of the interaction between herbal compounds and a biological membrane.By combining the dynamics equilibrium morphology,the distribution of drugs inside and outside the biomembrane,and the interaction sites of drugs on the DPPC bilayer,factors influencing transmembrane transport of drugs were elucidated and the function of platycodin D in a biological membrane was reproduced.展开更多
The complex morphological,anatomical,physiological,and chemical mechanisms within the aging brain have been the hot topic of research for centuries.The aging process alters the brain structure that affects functions a...The complex morphological,anatomical,physiological,and chemical mechanisms within the aging brain have been the hot topic of research for centuries.The aging process alters the brain structure that affects functions and cognitions,but the worsening of such processes contributes to the pathogenesis of neurodegenerative disorders,such as Alzheimer's disease.Beyond these observable,mild morphological shifts,significant functional modifications in neurotransmission and neuronal activity critically influence the aging brain.Understanding these changes is important for maintaining cognitive health,especially given the increasing prevalence of age-related conditions that affect cognition.This review aims to explore the age-induced changes in brain plasticity and molecular processes,differentiating normal aging from the pathogenesis of Alzheimer's disease,thereby providing insights into predicting the risk of dementia,particularly Alzheimer's disease.展开更多
Background: The mechanisms by which acupuncture affects poststroke cognitive impairment (PSCI) remain unclear. Objective: To investigate brain functional network (BFN) changes in patients with PSCI after acupuncture t...Background: The mechanisms by which acupuncture affects poststroke cognitive impairment (PSCI) remain unclear. Objective: To investigate brain functional network (BFN) changes in patients with PSCI after acupuncture therapy. Methods: Twenty-two PSCI patients who underwent acupuncture therapy in our hospital were enrolled as research subjects. Another 14 people matched for age, sex, and education level were included in the normal control (HC) group. All the subjects underwent resting-state functional magnetic resonance imaging (rs-fMRI) scans;the PSCI patients underwent one scan before acupuncture therapy and another after. The network metric difference between PSCI patients and HCs was analyzed via the independent-sample t test, whereas the paired-sample t test was employed to analyze the network metric changes in PSCI patients before vs. after treatment. Results: Small-world network attributes were observed in both groups for sparsities between 0.1 and 0.28. Compared with the HC group, the PSCI group presented significantly lower values for the global topological properties (γ, Cp, and Eloc) of the brain;significantly greater values for the nodal attributes of betweenness centrality in the CUN. L and the HES. R, degree centrality in the SFGdor. L, PCG. L, IPL. L, and HES. R, and nodal local efficiency in the ORBsup. R, ORBsupmed. R, DCG. L, SMG. R, and TPOsup. L;and decreased degree centrality in the MFG. R, IFGoperc. R, and SOG. R. After treatment, PSCI patients presented increased degree centrality in the LING.L, LING.R, and IOG. L and nodal local efficiency in PHG. L, IOG. R, FFG. L, and the HES. L, and decreased betweenness centrality in the PCG. L and CUN. L, degree centrality in the ORBsupmed. R, and nodal local efficiency in ANG. R. Conclusion: Cognitive decline in PSCI patients may be related to BFN disorders;acupuncture therapy may modulate the topological properties of the BFNs of PSCI patients.展开更多
BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a cancer with a poor prognosis,characterized by distinct geographical distribution and family clustering.AIM To investigate if ethnic differences(Han vs Kazakh)cau...BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a cancer with a poor prognosis,characterized by distinct geographical distribution and family clustering.AIM To investigate if ethnic differences(Han vs Kazakh)cause molecular variations in ESCC patients via genomic sequencing 299 samples.METHODS Here,we sequenced samples from 299 ESCC patients collected from Henan Key Laboratory for Esophageal Cancer Research and National Key Laboratory of Metabolic Dysregulation and Esophageal Cancer Prevention and Treatment,The First Affiliated Hospital of Zhengzhou University,including Han and Kazakh ethnic groups,and performed a genomic comparative analysis of these two ethnic cohorts.RESULTS ESCC patients of Kazakh ethnicity present with a later age of onset compared to Han.Kazakh patients exhibit a slightly higher tumor mutation burden compared to their Han counterparts.Three genes GIGYF1,CACNA1D,and ACOT11 exhibited mutation frequencies threefold higher in Kazakh patients than in Han.This enrichment may be associated with Kazakhs’adaptation to cold climates and consumption of high-calorie diets.Among Han patients,the apolipoprotein B messenger RNA-editing enzyme catalytic polypeptide(APOBEC)-associated single base substitutions(SBS)13 mutational signature is more prevalent,whereas SBS6,indicative of DNA mismatch repair deficiency,is more common in Kazakh patients.Additionally,Han Chinese patients with APOBEC-enriched tumors exhibit a significantly higher mutation load than those without.Moreover,patients lacking the APOBEC signature demonstrate superior survival probability compared to the APOBEC-enriched group.CONCLUSION Living environment and diet are major factors in the development of ESCC.Genomic difference may provide guidance for the formulation of clinical treatment plans for ESCC from different ethnics regions.展开更多
MnCO_(3)represents a potentially high-capacity and low-cost anode candidate to replace graphite for enhancing energy density of commercial lithium-ion batteries,but it suffers from poor electrical conductivity and ser...MnCO_(3)represents a potentially high-capacity and low-cost anode candidate to replace graphite for enhancing energy density of commercial lithium-ion batteries,but it suffers from poor electrical conductivity and serious volumetric change,largely hindering its practical applications.展开更多
基金Supported by the Hebei Province Natural Science Foundation,No.H2018206034 and No.H2022206544Hebei Province clinical medicine outstanding personnel training project,No.ZF2024135.
文摘BACKGROUND Pharmacological treatments are commonly used in individuals experiencing perinatal depression(PPD);however,a debate regarding the reproductive safety of antidepressants is ongoing.Many pregnant women opt to discontinue antidepressant out of concern about potential negative effects on the developing fetus,while slow and ineffective antidepressant medications hinder improved outcomes in women with PPD.In recent years,bright light therapy(BLT)has gained traction as a treatment option for PPD;however,clinical trials findings examining the efficacy of BLT in this population have been inconclusive.AIM To validate the feasibility and safety of BLT for the treatment of PPD.METHODS We performed a meta-analysis of randomized controlled trials of patients with PPD treated with BLT vs placebo following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis.We searched PubMed,Embase,the Cochrane Library,and Web of Science for randomized controlled studies published up to December 2023.The results were evaluated using the standardized mean difference of improvement for depression scores and odds ratios(ORs)for remission rate,response rate,incidence of adverse events,and dropout rate.RESULTS The BLT group had higher PPD response rate[50.68%vs 33.08%;OR=2.05;95% confidence interval(CI):1.25-3.35;P=0.004;I^(2)=35%]and remission rate(54.10%vs 18.52%;OR=5.00;95%CI:2.09-11.99;P=0.0003;I^(2)=0%)than the placebo group.Improvements in depression scores were higher in the BLT group than the placebo group for the overall efficacy(standardized mean difference=-0.47;95%CI:-0.80 to-0.13;P=0.007).No significant differences between the two groups in drop-outs(21.84%vs 29.63%;OR=0.63;95%CI:0.31-1.29;P=0.21;I^(2)=0%)or adverse events(17.89%vs 9.68%;OR=2.01;95%CI:0.95-4.25;P=0.07;I^(2)=0%)were observed.CONCLUSION BLT can potentially treat PPD,showing better results than the control group in this study.BLT is effective and safe and could increase the available therapeutic options for PPD.
基金funded by the National Key Research and Development Program(Grant No.2022YFD1600700)Major Science and Technology Projects of Henan Province(Grant No.221100110400)+3 种基金the China Agriculture Research System of MOF and MARA(Grant No.CARS-26)Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Science(Grant No.CAAS-ASTIP-2023-ZFRI-03)Yunnan Science and Technology Program(Grant No.202205AF150043)Sichuan Science and Technology Program(Grant No.2021YFN0060)。
文摘Waterlogging stress is one of the greatest environmental threats to kiwifruit growth and development.ERF-VII proteins have been demonstrated to play pivotal roles in regulating plant tolerance to waterlogging.Nevertheless,the genome-wide role of ERF-VII in kiwifruit waterlogging stress tolerance remains unclear.Here,we report the function and regulatory network of an ERF-VII transcription factor located to the nucleus,Av ERF73,in kiwifruit waterlogging tolerance.Overexpression of Av ERF73 in Arabidopsis thaliana and A.chinensis cv.Hongyang enhanced waterlogging tolerance in transgenic plants.Furthermore,we performed transcriptome analysis(RNA-seq)and DNA affinity purification sequencing(DAP-seq)to explore the regulatory mechanism of Av ERF73.RNA-seq coupled with DAP-seq showed that Av ERF73 might directly activate Ac NAC022 involved in the“cellular response to hypoxia”process and Ac HMGS1 involved in the mevalonate pathway to respond to waterlogging,which were also confirmed by a dual-luciferase reporter assay.Based on our results,we propose a putative working model for controlling waterlogging tolerance by Av ERF73 in kiwifruit.
基金supported by the China Agriculture Research System of MOF and MARA(CARS-21)the Central Public-interest Scientific Institution Basal Research Fund(Y2022QC12)the National Natural Science Foundation of China(32272443)。
文摘Lycium barb arum,known as wolfberry or goji berry,is consumed by humans as a medicine and a food homology product.Conventionally grown wolfberry is often treated extensively with pesticides,which could pose a hazard to humans.Here,the degradation dynamics of dinotefuran and its 2 metabolites(1-methyl-3-(tetrahydro-3-furylmethyl)urea(UF)and 1-methyl-3-(tetrahydro-3-furylmethyl)guanidine(DN)),during wolfberry cultivation and processing was investigated.The half-life(T_(1/2))of dinotefuran was 11.36 and 9.76 days,respectively,under the recommended dosage and double the recommended dosage.During the oven and sun drying processes,processing factors(PFs)of dinotefuran were 1.07-1.34,implying the enrichment of pesticide residues.Decoction process made the removal rate of dinotefuran reach 87.48%,which is higher than that of the brewing process(14.7%),while dinotefuran remained in the wine with high ethanol content in the alcohol soaking process.The hazard quotient(HQ)of dinotefuran,as determined via dietary risk assessment combined with PFs,was<1,indicating an acceptable risk for human consumption.Bioaccessibility of dinotefuran in the three digestive stages were intestinal(18.20%-88.08%)>gastric(5.45%-86.72%)>oral(23.18%)via in vitro simulated digestive system.These findings provide scientific evidence for reasonable application and risk assessment of dinotefuran residues in wolfberry.
基金funded by the Earmarked Fund for CARS(CARS-28-07)the Agricultural Variety Improvement Project of Shandong Province,China(2022LZGC011)the Qingdao Agricultural University Doctoral Start-Up Fund,China.
文摘Dehydrin(DHN)enhances plant resistance to environmental stress by regulating the synthesis of osmotic adjustment substances and scavenging reactive oxygen species.However,the role of PbDHN3 under salt stress remains unclear.In this study,salt stress induced high expression of PbDHN3,and the overexpression of PbDHN3(OE-PbDHN3)enhanced plant growth under salt stress compared to wild-type(WT)plants.OE-PbDHN3 plants exhibited higher chlorophyll content and root growth capacity than WT plants under salt stress.Transcriptome analysis revealed that PbDHN3 expression is associated with ethylene signaling pathways.OE-PbDHN3 transgenic plants substantially influenced ethylene content and the expression of related genes.Following treatment with exogenous ethephon,the transgenic lines notably inhibited the processes of ethylene synthesis and signaling transduction.OE-PbDHN3 transgenic lines treated with exogenous ethylene and the ethylene inhibitor 1-MCP demonstrated significant inhibition of ethylene synthesis and signaling transduction,while promoting root development and chlorophyll content.Under salt stress,OE-PbDHN3 downregulated the expression of ethylene biosynthesis genes PbACO1-like and PbACO2,and signal transduction genes PbEIN3-like during the initial stress phase.This early regulation mitigated the adverse effects of salt stress on the plants.These findings demonstrate that PbDHN3 ameliorates the ethylene-mediated plant growth phenotype under salt stress through regulation of ethylene synthesis and signal transduction.
基金partly supported by research grants from the Outstanding Youth Foundation of the Beijing Academy of Agriculture and Forestry Sciences,China(YXQN202301)the National Natural Science Foundation of China(32272522)the Beijing Natural Science Foundation,China(6232005)。
文摘The tobacco whitefly,Bemisia tabaci,is a notorious pest affecting various crops globally,and it exhibits high levels of resistance to various insecticides.Afidopyropen is a recently commercialized pyropene insecticide for B.tabaci control with high selectivity and a novel mode of action.We previously identified a high level of afidopyropen resistance in a field-collected population after selection in the lab,and named it the HD-Afi strain.In the present study,minimal cross-resistance in the HD-Afi strain was found between afidopyropen and other common chemical agents.However,the P450 enzyme activity in HD-Afi was 2.18 times the level in susceptible strain HD-S.Expression analysis revealed that two of 12 candidate P450 genes,namely CYP6DW3 and CYP4C64,were significantly up-regulated in HD-Afi.Silencing CYP6DW3 and CYP4C64 by RNA interference(RNAi)substantially increased the susceptibility of whitefly adults,confirming their involvement in afidopyropen resistance.Homology modeling and molecular docking analyses demonstrated stable binding of afidopyropen to CYP6DW3 and CYP4C64,with binding free energies of–6.87 and–6.11 kcal mol^(-1),respectively.The findings of this study suggest that the induction of CYP6DW3 and CYP4C64 facilitates afidopyropen detoxification,contributing to the development of resistance in B.tabaci.
文摘Background: Intravoxel incoherent motion diffusion weighted imaging (IVIM-DWI) can not only observe the diffusion of tissue water molecules but also reflect the blood perfusion information of tissue microcirculation. IVIM-DWI has been applied in many clinical areas. However, few studies have addressed the use of IVIM-DWI for the evaluation of transarterial chemoembolization (TACE) response in hepatocellular carcinoma (HCC) patients. Objectives: The purpose of the present study was to explore the role of IVIM-DWI for the therapeutic response of TACE for HCC. Materials and Methods: Twenty patients underwent IVIM-DWI scan on a 3.0T magnetic resonance imaging instrument 1 - 3 days before and 30 to 40 days after TACE. The values of IVIM-DWI parameters, including standard apparent diffusion coefficient (ADC), pure diffusion coefficient (Dslow), pseudo-diffusion coefficient (Dfast) and perfusion fraction (f) were measured. The values of IVIM-DWI parameters before and after TACE were compared using paired t tests. The values between responsive and non-responsive groups were compared using independent-samples t test. P 0.05 indicated statistical significance. Results: After TACE, the ADC and Dslow values in the tumors increased significantly, and the values of Dfast decreased significantly, while the values of f value did not change obviously. The ADC values in responsive group were remarkably higher than those in non-responsive group, the Dfast values in responsive group were significantly lower than those in non-responsive group, but the values of Dslow and f between the two groups were not different significantly. Conclusions: IVIM-DWI parameters can be used as potential markers for the therapeutic response of TACE for HCC.
基金National Natural Science Foundation of China (Nos.11672069, 11702059,11232003,11672062)the Ph.D.Programs Foundation of Ministry of Education of China (No.20130041110050)+3 种基金the Research Start-up Project Plan for Liaoning Doctors (No.20141119)the Fundamental Research Funds for the Cen- tral Universities (No.DC201502050407,DC201502050203,DC201502050403,20000101)the Natural Science Foundation of Liaoning Province (No.20170540199)and the 111Project (B08014).
文摘In this paper,the bounded traveling wave solutions are examined for a system of partial differential equations with certain boundary conditions.Interestingly,this system can be used to describe the axially symmetric motion of a semi-infinite incompressible hyperelastic cylindrical rod.With the aid of traveling wave transformations,the partial differential equations can be reduced to a traveling wave equation.The implicit analytical expressions determining the traveling waves are derived.Significantly,the influences of material parameters on the qualitative properties are discussed in detail by using the phase portraits of the traveling wave system. Smooth solutions such as the periodic traveling wave solutions and the solitary wave solutions with the peak form are shown.In particular,some interesting singular traveling wave solutions including the solitary cusp wave solutions and the periodic cusp wave solutions with the peak form are obtained.Numerical examples for all these waves are given.
基金This work is supported by the National Natural Science Foundation of China(Nos.11672069,11702059,11232003,11672062)The Ph.D.Programs Foundation of Ministry of Education of China(No.20130041110050)+3 种基金the Research Startup Project Plan for Liaoning Doctors(No.20141119)the Fundamental Research Funds for the Central Universities(20000101)the Natural Science Foundation of Liaoning Province(No.20170540199)111 Project(B08014).
文摘In this paper,a nonlinear wave equation with variable coefficients is studied,interestingly,this equation can be used to describe the travelling waves propagating along the circular rod composed of a general compressible hyperelastic material with variable cross-sections and variable material densities.With the aid of Lou’s direct method1,the nonlinear wave equation with variable coefficients is reduced and two sets of symmetry transformations and exact solutions of the nonlinear wave equation are obtained.The corresponding numerical examples of exact solutions are presented by using different coefficients.Particularly,while the variable coefficients are taken as some special constants,the nonlinear wave equation with variable coefficients reduces to the one with constant coefficients,which can be used to describe the propagation of the travelling waves in general cylindrical rods composed of generally hyperelastic materials.Using the same method to solve the nonlinear wave equation,the validity and rationality of this method are verified.
基金This research was financially supported by the China Geological Survey(DD20160049).
文摘1.Objective The study area is located at the southern Zhangguangcai range,which is thought to be the eastern part of the Central Asian Orogenic Belt(CAOB).The study area is closed to the eastern margin of the Songliao basin(Fig.1a).No petroleum exploration or related research work have been conducted in this area by far.Based on our most recent geological mapping,numerous hydrocarbon source rocks were firstly discovered within the Xinxing Formation,which is widely exposed from the central Jilin Province to the southeastern Heilongjiang province.The Xinxing Formation covers an area of nearly 1000 km2 with a total thickness of more than 1481 m(Regional Geology of Heilongjiang Province,1993).Therefore,the Xinxing Formation has huge potential to form oil and gas resources.This new discovery of hydrocarbon source rocks within the Xinxing Formation could provide critical clues for oil and gas resources’investigation in Northeast China,also may has important economic significance.
基金supported in part by grants from the National Key Research and Development Program of China(2021YFD1600204 and 2023YFF1104500)the 111 project from the Ministry of Education of the People’s Republic of China 577(B18053).
文摘Skin aging is an increasingly prominent topic in the context of healthy aging.During the aging process,the skin’s barrier function diminishes,its water content decreases,wrinkles begin to form,and changes occur in the gut microbiota composition.However,the relationship between gut microbiota and skin aging remains unclear.In this study,we explored skin rejuvenation in aged mice through fecal microbiota trans-plantation(FMT)using feces from young mice.The results demonstrated enhanced water retention,thick-ened stratum corneum,increased collagen content,and improved epithelial cell differentiation in aged mice following FMT.Notably,FMT particularly increased the abundance of Lactobacillus and Lactococcus in aged mice,which were nearly undetectable in untreated aged mice.Non-targeted and targeted meta-bolomics analyses indicated that FMT significantly elevated levels of tryptophan(Trp)and its microbiota metabolites(e.g.,indole-3-lactic acid(ILA))in the feces and serum of aged mice.Both Trp and ILA appeared to rejuvenate aged skin by activating the aryl hydrocarbon receptor(AhR)to promote epidermal cell dif-ferentiation.In conclusion,FMT from young mice rejuvenated aged skin via Trp-metabolizing bacteria(Lactobacillus and Lactococcus)and Trp-derived metabolites,suggesting that interventions targeting Trp metabolites may effectively improve skin aging.
基金supported by Beijing Natural Science Foundation (KZ201810028044)。
文摘Well-ordered hierarchically mesoporous/microporous carbon materials have been successfully fabricated by using dual soft-templating approach through compressed CO_(2).Pluronic F127 and different type of surfactants,including nonionic,cationic,and anionic surfactants,were used as dual templates to investigate the influence on the morphology and nanostructure of the as-prepared carbon samples.TEM,SEM,N2 sorption,wide-angle and small-angle XRD analysis were employed to reveal the well-ordered hierarchically micro-mesoporous structure with2 D hexagonal symmetry by using compressed CO_(2).The prepared HPC samples with different pressures as the catalyst carriers have been functioned by chlorosulfonic acid for the fructose conversion into HMF.Chlorosulfonic acid concentration,catalyst dosage and reaction temperature have been optimized for fructose-to-HMF transformation with the obtained catalyst.The performances of as-made HPC-SO_(3) H samples in HMF yield and reaction rate of fructose-to-HMF transformation have been investigated.The stability of the samples was also conducted in the dehydration of fructose to HMF for five cycles.The possible catalytic mechanism by using hierarchically porous carbon materials as catalyst support for fructose-to-HMF transformation was proposed.
基金Supported by the National Natural Science Foundation of China(21776217,21506160)Tianjin Research Program of Application Foundation and Advanced Technology(14JCQNJC06400)+1 种基金the Scientific Research Foundation for the Returned Overseas Chinese Scholars(48)the Science and Technology Plans of Tianjin(16PTSYJC00110)
文摘Nanostructured zeolitic imidazolate frameworks(ZIF-8) was incorporated into the mixture of poly(ethylene glycol) methyl ether acrylate(PEGMEA) and pentaerythritol triacrylate(PETA) to synthesize mixed matrix membranes(MMMs) by in situ polymerization for CO_2/CH_4 separation. The solvent-free polymerization between PEGMEA and PETA was induced by UV light with 1-hydroxylcyclohexyl phenyl ketone as initiator. The chemical structural characterization was performed by Fourier transform infrared spectroscopy. The morphology was characterized by scanning electron microscope. The average chain-to-chain distance of the polymer chains in MMMs was investigated by X-ray diffraction. The thermal property was evaluated by differential scanning calorimetry. The CH_4 and CO_2 gas transport properties of MMMs are reported. The relationship between gas permeation–separation performances or physical properties and ZIF-8 loading is also discussed. However, the permeation–separation performance was not improved in Robeson upper bound plot compared with original polymer membrane as predicted. The significant partial pore blockage and polymer rigidification effect around the ZIFs confirmed by the increase in glass temperature and the decrease in the d-spacing, were mainly responsible for the failure in performance improvement, which offset the high diffusion induced by porous ZIF-8.
基金The experiment was financially supported by the National Natural Science Foundation of China(81473364)Beijing Natural Science Foundation(7162122)Excellent Talents Training Subsidy Scheme of Beijing(2013D009999000003).
文摘Objective:To study the transmembrane transport of chemical components of Chinese herbs and to explore the function of platycodin D (PD) on biomembranes.Methods:Interaction between PD and the dipalmitoylphosphatidylcholine (DPPC) bilayer was reproduced by molecular dynamics simulation with the Martini force field.A model validation and methodological study were first performed,and were based on simulation investigations of transmembrane transport for three herbal compounds with distinct hydrophilic properties.Results:PD increased the mobility of the DPPC bilayer since its aglycone strongly interacted with the hydrophobic layer,which broke the structure of the gate layer,and weakened the ordered performance of hydrophobic tails.Conclusion:The Martini force field was successfully applied to the study of the interaction between herbal compounds and a biological membrane.By combining the dynamics equilibrium morphology,the distribution of drugs inside and outside the biomembrane,and the interaction sites of drugs on the DPPC bilayer,factors influencing transmembrane transport of drugs were elucidated and the function of platycodin D in a biological membrane was reproduced.
文摘The complex morphological,anatomical,physiological,and chemical mechanisms within the aging brain have been the hot topic of research for centuries.The aging process alters the brain structure that affects functions and cognitions,but the worsening of such processes contributes to the pathogenesis of neurodegenerative disorders,such as Alzheimer's disease.Beyond these observable,mild morphological shifts,significant functional modifications in neurotransmission and neuronal activity critically influence the aging brain.Understanding these changes is important for maintaining cognitive health,especially given the increasing prevalence of age-related conditions that affect cognition.This review aims to explore the age-induced changes in brain plasticity and molecular processes,differentiating normal aging from the pathogenesis of Alzheimer's disease,thereby providing insights into predicting the risk of dementia,particularly Alzheimer's disease.
文摘Background: The mechanisms by which acupuncture affects poststroke cognitive impairment (PSCI) remain unclear. Objective: To investigate brain functional network (BFN) changes in patients with PSCI after acupuncture therapy. Methods: Twenty-two PSCI patients who underwent acupuncture therapy in our hospital were enrolled as research subjects. Another 14 people matched for age, sex, and education level were included in the normal control (HC) group. All the subjects underwent resting-state functional magnetic resonance imaging (rs-fMRI) scans;the PSCI patients underwent one scan before acupuncture therapy and another after. The network metric difference between PSCI patients and HCs was analyzed via the independent-sample t test, whereas the paired-sample t test was employed to analyze the network metric changes in PSCI patients before vs. after treatment. Results: Small-world network attributes were observed in both groups for sparsities between 0.1 and 0.28. Compared with the HC group, the PSCI group presented significantly lower values for the global topological properties (γ, Cp, and Eloc) of the brain;significantly greater values for the nodal attributes of betweenness centrality in the CUN. L and the HES. R, degree centrality in the SFGdor. L, PCG. L, IPL. L, and HES. R, and nodal local efficiency in the ORBsup. R, ORBsupmed. R, DCG. L, SMG. R, and TPOsup. L;and decreased degree centrality in the MFG. R, IFGoperc. R, and SOG. R. After treatment, PSCI patients presented increased degree centrality in the LING.L, LING.R, and IOG. L and nodal local efficiency in PHG. L, IOG. R, FFG. L, and the HES. L, and decreased betweenness centrality in the PCG. L and CUN. L, degree centrality in the ORBsupmed. R, and nodal local efficiency in ANG. R. Conclusion: Cognitive decline in PSCI patients may be related to BFN disorders;acupuncture therapy may modulate the topological properties of the BFNs of PSCI patients.
基金Supported by the National Natural Science Foundation of China,No.U1301227National Major Science and Technology Projects,No.2024ZD05209060Henan Province Jointly Built Science and Technology Key Projects,No.LHGJ20210337.
文摘BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a cancer with a poor prognosis,characterized by distinct geographical distribution and family clustering.AIM To investigate if ethnic differences(Han vs Kazakh)cause molecular variations in ESCC patients via genomic sequencing 299 samples.METHODS Here,we sequenced samples from 299 ESCC patients collected from Henan Key Laboratory for Esophageal Cancer Research and National Key Laboratory of Metabolic Dysregulation and Esophageal Cancer Prevention and Treatment,The First Affiliated Hospital of Zhengzhou University,including Han and Kazakh ethnic groups,and performed a genomic comparative analysis of these two ethnic cohorts.RESULTS ESCC patients of Kazakh ethnicity present with a later age of onset compared to Han.Kazakh patients exhibit a slightly higher tumor mutation burden compared to their Han counterparts.Three genes GIGYF1,CACNA1D,and ACOT11 exhibited mutation frequencies threefold higher in Kazakh patients than in Han.This enrichment may be associated with Kazakhs’adaptation to cold climates and consumption of high-calorie diets.Among Han patients,the apolipoprotein B messenger RNA-editing enzyme catalytic polypeptide(APOBEC)-associated single base substitutions(SBS)13 mutational signature is more prevalent,whereas SBS6,indicative of DNA mismatch repair deficiency,is more common in Kazakh patients.Additionally,Han Chinese patients with APOBEC-enriched tumors exhibit a significantly higher mutation load than those without.Moreover,patients lacking the APOBEC signature demonstrate superior survival probability compared to the APOBEC-enriched group.CONCLUSION Living environment and diet are major factors in the development of ESCC.Genomic difference may provide guidance for the formulation of clinical treatment plans for ESCC from different ethnics regions.
基金supported by the National Natural Science Foundation of China(Nos.52102088 and 22075026)support from Teli Fellowship,Beijing Institute of Technology,and facility support from Analysis&Testing Center,and Experimental Center of Materials Sciences&Engineering at Beijing Institute of Technology.
文摘MnCO_(3)represents a potentially high-capacity and low-cost anode candidate to replace graphite for enhancing energy density of commercial lithium-ion batteries,but it suffers from poor electrical conductivity and serious volumetric change,largely hindering its practical applications.
