Objective:To formulate and evaluate acyclovir microcapsules using bakers yeast.Methods: Acyclovir,pretreated yeast and deionised water were taken at a volumetric ratio of 1:2:4 respectively.This suspension was agitate...Objective:To formulate and evaluate acyclovir microcapsules using bakers yeast.Methods: Acyclovir,pretreated yeast and deionised water were taken at a volumetric ratio of 1:2:4 respectively.This suspension was agitated in a magnetic stirrer at 25℃30℃.35℃,and 40℃for 4 hours.The suspension was then centrifuged for 10 minutes at 2 000 rpm.The supernatant solution was decanted and the cells were washed 5 times with deionised water.Then the suspended drug entrapped yeast cells were dried in a lyophillizer for 48 hours.The yield was noted.Results:The first four formulations were done with 200 mg of the drug,followed by 400 mg for the next four formulations and 800 mg the last four formulations.SEM showed that the surface of the microcapsules was intact,with no burst characteristics.FTIR showed no interaction between acyclovir and the cell wall.DSC showed that the peak was within the standard values. The mean particle size for all the samples was 8μm in diameter.The dissolution studies were done for all the twelve samples and showed a Fickian model of diffusion.Conclusions: From the results it is inferred that the samples prepared at 40℃(FY-4,FY- 8,FY-12) show better entrapment and release.So these samples are formulated in the form of a suspension and compared with marketed acyclovir suspension using HPLC technique.The formulated suspensions with FY-4,FY-8 and FY-12 shows drug content in accordance with the standards of the pharmacopoeial limits.展开更多
文摘Objective:To formulate and evaluate acyclovir microcapsules using bakers yeast.Methods: Acyclovir,pretreated yeast and deionised water were taken at a volumetric ratio of 1:2:4 respectively.This suspension was agitated in a magnetic stirrer at 25℃30℃.35℃,and 40℃for 4 hours.The suspension was then centrifuged for 10 minutes at 2 000 rpm.The supernatant solution was decanted and the cells were washed 5 times with deionised water.Then the suspended drug entrapped yeast cells were dried in a lyophillizer for 48 hours.The yield was noted.Results:The first four formulations were done with 200 mg of the drug,followed by 400 mg for the next four formulations and 800 mg the last four formulations.SEM showed that the surface of the microcapsules was intact,with no burst characteristics.FTIR showed no interaction between acyclovir and the cell wall.DSC showed that the peak was within the standard values. The mean particle size for all the samples was 8μm in diameter.The dissolution studies were done for all the twelve samples and showed a Fickian model of diffusion.Conclusions: From the results it is inferred that the samples prepared at 40℃(FY-4,FY- 8,FY-12) show better entrapment and release.So these samples are formulated in the form of a suspension and compared with marketed acyclovir suspension using HPLC technique.The formulated suspensions with FY-4,FY-8 and FY-12 shows drug content in accordance with the standards of the pharmacopoeial limits.
