The aim of this study is to fabricate a multifunctional biohybrid composite utilizing double walled carbon nanotubes(DWCNTs)as a carrier for loading of the colistin sulfate(CLS)through the application of the sol-gel t...The aim of this study is to fabricate a multifunctional biohybrid composite utilizing double walled carbon nanotubes(DWCNTs)as a carrier for loading of the colistin sulfate(CLS)through the application of the sol-gel technique.The resulting composite was analyzed by Fourier transform infrared spectroscopy(FTIR),thermogravimetric analysis(TGA),scanning electron microscope(SEM),X-ray diffraction patterns(XRD),and particle size distribution analysis utilizing dynamic light scattering technique(DLS).Furthermore,an assessment of in vitro bone bioactivity was conducted under physiological levels of Ca2+and PO4+(SBF)at 37°C and pH 7.4 over a period of 7 days.Additionally,the in vitro cytotoxicity against mice bone marrow cells was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.The outcomes indicated that the composite possessed a particle size of 365 nm in diameter with a PDI of 0.21(the pure CLS and untreated DWCNTs were 290 and 459 nm in diameter,respectively),improved biocompatibility,and promoted the formation of a Ca-phosphate apatite layer on the surface with a Ca/P ratio of approximately 1.78.The findings collectively validated the significant contribution of the CLS peptide in DWCNTs-based composite for the purposes of bone tissue engineering.展开更多
基金supported by the National Research Centre,Egypt,according to the project No.13020207.
文摘The aim of this study is to fabricate a multifunctional biohybrid composite utilizing double walled carbon nanotubes(DWCNTs)as a carrier for loading of the colistin sulfate(CLS)through the application of the sol-gel technique.The resulting composite was analyzed by Fourier transform infrared spectroscopy(FTIR),thermogravimetric analysis(TGA),scanning electron microscope(SEM),X-ray diffraction patterns(XRD),and particle size distribution analysis utilizing dynamic light scattering technique(DLS).Furthermore,an assessment of in vitro bone bioactivity was conducted under physiological levels of Ca2+and PO4+(SBF)at 37°C and pH 7.4 over a period of 7 days.Additionally,the in vitro cytotoxicity against mice bone marrow cells was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.The outcomes indicated that the composite possessed a particle size of 365 nm in diameter with a PDI of 0.21(the pure CLS and untreated DWCNTs were 290 and 459 nm in diameter,respectively),improved biocompatibility,and promoted the formation of a Ca-phosphate apatite layer on the surface with a Ca/P ratio of approximately 1.78.The findings collectively validated the significant contribution of the CLS peptide in DWCNTs-based composite for the purposes of bone tissue engineering.
