3D Bioprinting plays an irreplaceable role in bone tissue engineering. Shellac and curcumin are two natural compounds that are widely used in the food and pharmaceutical sectors. In this study, a new composite scaffol...3D Bioprinting plays an irreplaceable role in bone tissue engineering. Shellac and curcumin are two natural compounds that are widely used in the food and pharmaceutical sectors. In this study, a new composite scaffold with good biocompatibility and antibacterial ability was manufactured by adding shellac and curcumin into the traditional bone scaffold through low-temperature three-dimensional printing (LT-3DP), and its impact on the osteoimmune microenvironment was evaluated.展开更多
Skin is the largest organ in human body,and it plays an important role in regulating physiological microenvironments and acts as a barrier to protect human body from harmful intrusions.The demand for fully functional ...Skin is the largest organ in human body,and it plays an important role in regulating physiological microenvironments and acts as a barrier to protect human body from harmful intrusions.The demand for fully functional skin models(also called skin equivalents,SE)in an in-vivo mimicking culturing microenvironment has been increased dramatically due to the fast development in skin disease treatments and skin care products.Owing to the emerging of the concept and technology of organ-on-chips along with the three-dimensional(3D)bioprinting technology,3D skin models and their applications have been fast evolving.In this paper,the advances in the development of 3D skin models along with skin-on-a-chip(SOC)are reviewed and commented.One of the findings with this paper is that the SOC together with the 3D bioprinting technology is promising to construct fully functional 3D skin models in the field of pharmaceutical and cosmetic industries.展开更多
Objective:Oncocardiology is increasingly hot research field/topic in the clinical management of cancer with anti-angiogenic therapy of vascular endothelial growth factor(VEGF)that may cause cardiovascular toxicity,suc...Objective:Oncocardiology is increasingly hot research field/topic in the clinical management of cancer with anti-angiogenic therapy of vascular endothelial growth factor(VEGF)that may cause cardiovascular toxicity,such as hypertension via vascular dysfunction and attenuation of eNOS/NO signaling in the baroreflex afferent pathway.The aim of the current study was to evaluate the potential roles of VEGF/VEGF receptors(VEGFRs)expressed in the baroreflex afferent pathway in autonomic control of blood pressure(BP)regulation.Methods:The distribution and expression of VEGF/VEGFRs were detected in the nodose ganglia(NG)and nucleus of tractus solitary(NTS)using immunostaining and molecular approaches.The direct role of VEGF was tested by NG microinjection under physiological and hypertensive conditions.Results:Immunostaining data showed that either VEGF or VEGFR2/VEGFR3 was clearly detected in the NG and NTS of adult male rats.Microinjection of VEGF directly into the NG reduced the mean blood pressure(MBP)dose-dependently,which was less dramatic in renovascular hypertension(RVH)rats,suggesting the VEGF-mediated depressor response by direct activation of the 1st-order baroreceptor neurons in the NG under both normal and disease conditions.Notably,this reduced depressor response in RVH rats was directly caused by the downregulation of VEGFR2,which compensated the up regulation of VEGF/VEGFR3 in the NG during the development of hypertension.Conclusion:It demonstrated for the first time that the BP-lowering property of VEGF/VEGFRs signaling via the activation of baroreflex afferent function may be a common target/pathway leading to BP dysregulation in anti-angiogenic therapy.展开更多
The rapid miniaturization of electronic devices has fueled unprecedented demand for flexible,high-performance sensors across fields ranging from medical devices to robotics.Despite advances in fabrication techniques,t...The rapid miniaturization of electronic devices has fueled unprecedented demand for flexible,high-performance sensors across fields ranging from medical devices to robotics.Despite advances in fabrication techniques,the development of micro-and nano-scale flexible force sensors with superior sensitivity,stability,and biocompatibility remains a formidable challenge.In this study,we developed a novel conductive photosensitive resin specifically designed for two-photon polymerization,systematically optimized its printing parameters,and improved its structural design,thereby enabling the fabrication of high-precision micro-spring force sensors(MSFS).The proposed photosensitive resin,doped with MXene nanomaterials,combines exceptional mechanical strength and conductivity,overcoming limitations of traditional materials.Using a support vector machine model in machine learning techniques,we optimized the polymerizability of the resin under varied laser parameters,achieving a predictive accuracy of 92.66%.This model significantly reduced trial-and-error in the TPP process,accelerating the discovery of ideal fabrication conditions.Finite element analysis was employed to design and simulate the performance of the MSFS,guiding structural optimization to achieve high sensitivity and mechanical stability.The fabricated MSFS demonstrated outstanding electromechanical performance,with a sensitivity coefficient of 5.65 and a fabrication accuracy within±50 nm,setting a new standard for MSFS precision.This work not only pushes the boundaries of sensor miniaturization but also introduces a scalable,efficient pathway for the rapid design and fabrication of highperformance flexible sensors.展开更多
The demand for real-time feedback and miniaturization of sensing elements is a crucial issue in the treating vascular diseases with minimally invasive interventions.Here,Fabry–Perot microcavities fabricated via direc...The demand for real-time feedback and miniaturization of sensing elements is a crucial issue in the treating vascular diseases with minimally invasive interventions.Here,Fabry–Perot microcavities fabricated via direct laser writing using a two-photon polymerization technique on fiber tips are proposed,designed,simulated,and experimentally demonstrated as a miniature triaxial force sensor for monitoring real-time interactions between the tip of a guidewire and human blood vessels and tissues during minimally invasive surgeries.The sensor contains four fiber tip-based Fabry–Perot cavities,which can be seamlessly integrated into medical guidewires and achieves three-axis force decoupling through symmetrically arranged flexible structures.The results showed that the proposed sensor achieved a cross-sectional diameter of 890μm and a high sensitivity of about 85.16 nm/N within a range of 0 to 0.5 N with a resolution of hundreds of micro-Newtons.The proposed triaxial force sensor exhibits high resolution,good biocompatibility,and electromagnetic compatibility,which can be utilized as an efficient monitoring tool integrated into minimally invasive surgical intervention devices for biomedical applications.展开更多
文摘3D Bioprinting plays an irreplaceable role in bone tissue engineering. Shellac and curcumin are two natural compounds that are widely used in the food and pharmaceutical sectors. In this study, a new composite scaffold with good biocompatibility and antibacterial ability was manufactured by adding shellac and curcumin into the traditional bone scaffold through low-temperature three-dimensional printing (LT-3DP), and its impact on the osteoimmune microenvironment was evaluated.
文摘Skin is the largest organ in human body,and it plays an important role in regulating physiological microenvironments and acts as a barrier to protect human body from harmful intrusions.The demand for fully functional skin models(also called skin equivalents,SE)in an in-vivo mimicking culturing microenvironment has been increased dramatically due to the fast development in skin disease treatments and skin care products.Owing to the emerging of the concept and technology of organ-on-chips along with the three-dimensional(3D)bioprinting technology,3D skin models and their applications have been fast evolving.In this paper,the advances in the development of 3D skin models along with skin-on-a-chip(SOC)are reviewed and commented.One of the findings with this paper is that the SOC together with the 3D bioprinting technology is promising to construct fully functional 3D skin models in the field of pharmaceutical and cosmetic industries.
基金supported by the National Natural Science Foundation of China(31171122,81573431,81971326 for B.-y.,Li).
文摘Objective:Oncocardiology is increasingly hot research field/topic in the clinical management of cancer with anti-angiogenic therapy of vascular endothelial growth factor(VEGF)that may cause cardiovascular toxicity,such as hypertension via vascular dysfunction and attenuation of eNOS/NO signaling in the baroreflex afferent pathway.The aim of the current study was to evaluate the potential roles of VEGF/VEGF receptors(VEGFRs)expressed in the baroreflex afferent pathway in autonomic control of blood pressure(BP)regulation.Methods:The distribution and expression of VEGF/VEGFRs were detected in the nodose ganglia(NG)and nucleus of tractus solitary(NTS)using immunostaining and molecular approaches.The direct role of VEGF was tested by NG microinjection under physiological and hypertensive conditions.Results:Immunostaining data showed that either VEGF or VEGFR2/VEGFR3 was clearly detected in the NG and NTS of adult male rats.Microinjection of VEGF directly into the NG reduced the mean blood pressure(MBP)dose-dependently,which was less dramatic in renovascular hypertension(RVH)rats,suggesting the VEGF-mediated depressor response by direct activation of the 1st-order baroreceptor neurons in the NG under both normal and disease conditions.Notably,this reduced depressor response in RVH rats was directly caused by the downregulation of VEGFR2,which compensated the up regulation of VEGF/VEGFR3 in the NG during the development of hypertension.Conclusion:It demonstrated for the first time that the BP-lowering property of VEGF/VEGFRs signaling via the activation of baroreflex afferent function may be a common target/pathway leading to BP dysregulation in anti-angiogenic therapy.
文摘The rapid miniaturization of electronic devices has fueled unprecedented demand for flexible,high-performance sensors across fields ranging from medical devices to robotics.Despite advances in fabrication techniques,the development of micro-and nano-scale flexible force sensors with superior sensitivity,stability,and biocompatibility remains a formidable challenge.In this study,we developed a novel conductive photosensitive resin specifically designed for two-photon polymerization,systematically optimized its printing parameters,and improved its structural design,thereby enabling the fabrication of high-precision micro-spring force sensors(MSFS).The proposed photosensitive resin,doped with MXene nanomaterials,combines exceptional mechanical strength and conductivity,overcoming limitations of traditional materials.Using a support vector machine model in machine learning techniques,we optimized the polymerizability of the resin under varied laser parameters,achieving a predictive accuracy of 92.66%.This model significantly reduced trial-and-error in the TPP process,accelerating the discovery of ideal fabrication conditions.Finite element analysis was employed to design and simulate the performance of the MSFS,guiding structural optimization to achieve high sensitivity and mechanical stability.The fabricated MSFS demonstrated outstanding electromechanical performance,with a sensitivity coefficient of 5.65 and a fabrication accuracy within±50 nm,setting a new standard for MSFS precision.This work not only pushes the boundaries of sensor miniaturization but also introduces a scalable,efficient pathway for the rapid design and fabrication of highperformance flexible sensors.
基金Foundation of National Center for Translational Medicine(Shanghai)SHU Branch(SUITM-2023010)National Natural Science Foundation of China(62005153)。
文摘The demand for real-time feedback and miniaturization of sensing elements is a crucial issue in the treating vascular diseases with minimally invasive interventions.Here,Fabry–Perot microcavities fabricated via direct laser writing using a two-photon polymerization technique on fiber tips are proposed,designed,simulated,and experimentally demonstrated as a miniature triaxial force sensor for monitoring real-time interactions between the tip of a guidewire and human blood vessels and tissues during minimally invasive surgeries.The sensor contains four fiber tip-based Fabry–Perot cavities,which can be seamlessly integrated into medical guidewires and achieves three-axis force decoupling through symmetrically arranged flexible structures.The results showed that the proposed sensor achieved a cross-sectional diameter of 890μm and a high sensitivity of about 85.16 nm/N within a range of 0 to 0.5 N with a resolution of hundreds of micro-Newtons.The proposed triaxial force sensor exhibits high resolution,good biocompatibility,and electromagnetic compatibility,which can be utilized as an efficient monitoring tool integrated into minimally invasive surgical intervention devices for biomedical applications.
