BACKGROUND Endometriosis affects approximately 10%of reproductive-age women and is frequently associated with chronic pelvic pain.Patients with endometriosis often experience comorbid depression and anxiety,but the un...BACKGROUND Endometriosis affects approximately 10%of reproductive-age women and is frequently associated with chronic pelvic pain.Patients with endometriosis often experience comorbid depression and anxiety,but the underlying mechanisms connecting these conditions are unclear.AIM To assess the prevalence of depression and anxiety in endometriosis patients and explore neuroimmune mechanisms mediated via inflammatory biomarkers.METHODS A retrospective cohort study was conducted with 200 patients with endometriosis-associated chronic pain from June 2020 to December 2024.Depression and anxiety were assessed using validated psychological instruments.Inflammation biomarkers interleukin(ILs)(IL-6,IL-1β),tumor necrosis factor-alpha,C-reactive protein,and brain-derived neurotrophic factor were measured in serum.Pain severity was assessed using visual analog scales.Correlation and regression analyses were performed to examine relationships between inflammatory markers,pain severity,and psychological outcomes.RESULTS Among the 200 patients,42.5%exhibited clinically significant depression and 51.0%showed anxiety symptoms.Serum levels of IL-6,IL-1β,tumor necrosis factor-alpha,and C-reactive protein were significantly higher in patients with comorbid depression and anxiety compared with those without psychological symptoms(P<0.001).Brainderived neurotrophic factor levels were lower in the depression group.Pain severity positively correlated with inflammatory marker levels and with depression and anxiety scores.CONCLUSION Overall,the findings suggest that inflammatory factors mediate a neuroimmune mechanism linking endometriosisassociated chronic pain with depression and anxiety.Therapeutic targets for managing psychological comorbidities in patients with endometriosis through anti-inflammatory interventions should be explored,and an integrated treatment approach addressing both physical and psychological symptoms is emphasized.展开更多
BACKGROUND Currently,only tumors classified as LR-5 are considered definitive hepatocellular carcinoma(HCC),and no further pathologic confirmation is required to initiate therapy.Previous studies have shown that the s...BACKGROUND Currently,only tumors classified as LR-5 are considered definitive hepatocellular carcinoma(HCC),and no further pathologic confirmation is required to initiate therapy.Previous studies have shown that the sensitivity of LR-5 is modest,and lesions enhanced by gadoxetic acid(Gd-EOB-DTPA)may exhibit lower sensitivity than those enhanced by Gd-DTPA.AIM To identify malignant ancillary features(AFs)that can independently and significantly predict HCC in Liver Imaging Reporting and Data System version 2018,and to develop modified LR-5 criteria to improve diagnostic performance on Gd-EOB-DTPA-enhanced magnetic resonance imaging.METHODS Imaging data from patients with HCC risk factors who underwent abdominal Gd-EOB-DTPA-enhanced magnetic resonance imaging were collected.Univariate and multivariate logistic regression analyses were performed to determine AFs that could independently and significantly predict HCC.The modified LR-5 criteria involved reclassifying LR-4/LR-3 lesions based on major features combined with independently significant AFs for HCC,or by substituting threshold growth with significant AFs.McNemar's test was used to compare the diagnostic performance of the modified LR-5 criteria.RESULTS A total of 244 lesions from 216 patients were included.Transitional phase hypointensity,mild-moderate T2 hyperintensity,and fat in mass(more than adjacent liver)were identified as significant independent predictors of HCC.Using the modified LR-5 criteria(e.g.,LR-5-M1:LR-4+transitional phase hypointensity;LR-5-M4:LR-5 by transitional phase hypointensity instead of threshold growth;LR-5-M5:LR-5 by mild-moderate T2 hyperintensity instead of threshold growth;LR-5-M8:LR-3/LR-4+any two features of transitional phase hypointensity/mild-moderate T2 hyperintensity/fat in mass),sensitivities were significantly increased(88.5%-89.1%)compared to the standard LR-5(60.6%;all P values<0.05),while specificities(84.8%-89.9%)remained largely unchanged(93.7%;all P values>0.05).The LR-5-M8 criterion achieved the highest sensitivity.CONCLUSION Mild-moderate T2 hyperintensity,transitional phase hypointensity,and fat in mass are independent and significant predictors of HCC malignant AFs.The modified LR-5 criteria can improve sensitivity without significantly reducing specificity.展开更多
This study proposes a method for analyzing the security distance of an Active Distribution Network(ADN)by incorporating the demand response of an Energy Hub(EH).Taking into account the impact of stochastic wind-solar ...This study proposes a method for analyzing the security distance of an Active Distribution Network(ADN)by incorporating the demand response of an Energy Hub(EH).Taking into account the impact of stochastic wind-solar power and flexible loads on the EH,an interactive power model was developed to represent the EH’s operation under these influences.Additionally,an ADN security distance model,integrating an EH with flexible loads,was constructed to evaluate the effect of flexible load variations on the ADN’s security distance.By considering scenarios such as air conditioning(AC)load reduction and base station(BS)load transfer,the security distances of phases A,B,and C increased by 17.1%,17.2%,and 17.7%,respectively.Furthermore,a multi-objective optimal power flow model was formulated and solved using the Forward-Backward Power Flow Algorithm,the NSGA-II multi-objective optimization algo-rithm,and the maximum satisfaction method.The simulation results of the IEEE33 node system example demonstrate that after opti-mization,the total energy cost for one day is reduced by 0.026%,and the total security distance limit of the ADN’s three phases is improved by 0.1 MVA.This method effectively enhances the security distance,facilitates BS load transfer and AC load reduction,and contributes to the energy-saving,economical,and safe operation of the power system.展开更多
Vesicles of lipid bilayer can adopt a variety of shapes due to different coating proteins.The ability of proteins to reshape membrane is typically characterized by inducing spontaneous curvature of the membrane at the...Vesicles of lipid bilayer can adopt a variety of shapes due to different coating proteins.The ability of proteins to reshape membrane is typically characterized by inducing spontaneous curvature of the membrane at the coated area.BAR family proteins are known to have a crescent shape and can induce membrane curvature along their concaved body axis but not in the perpendicular direction.We model this type of proteins as a rod-shaped molecule with an orientation and induce normal curvature along its orientation in the tangential plane of the membrane surface.We show how a ring of these proteins reshapes an axisymmetric vesicle when the protein curvature or orientation is varied.A discontinuous shape transformation from a protrusion shape without a neck to a one with a neck is found.Increasing the rigidity of the protein ring is able to smooth out the transition.Furthermore,we show that varying the protein orientation is able to induce an hourglass-shaped neck,which is significantly narrower than the reciprocal of the protein curvature.Our results offer a new angle to rationalize the helical structure formed by many proteins that carry out membrane fission functions.展开更多
new species in the genus Amauromyza Hendel,1931,Amauromyza(Cephalomyza)wuyuanensis Fu&Shi sp.nov.,is described and illustrated.A.(Cephalomyza)karli(Hendel,1927)is newly discovered from China.So far,five species in...new species in the genus Amauromyza Hendel,1931,Amauromyza(Cephalomyza)wuyuanensis Fu&Shi sp.nov.,is described and illustrated.A.(Cephalomyza)karli(Hendel,1927)is newly discovered from China.So far,five species in the genus Amauromyza have been reported from China.A key to these species and a checklist with host plants and distributions are presented.展开更多
Parkinson’s disease(PD),a neurodegenerative disease that shows a high incidence in older individuals,is becoming increasingly prevalent.Unfortunately,there is no clinical cure for PD,and novel anti-PD drugs are there...Parkinson’s disease(PD),a neurodegenerative disease that shows a high incidence in older individuals,is becoming increasingly prevalent.Unfortunately,there is no clinical cure for PD,and novel anti-PD drugs are therefore urgently required.However,the selective permeability of the blood–brain barrier(BBB)poses a huge challenge in the development of such drugs.Fortunately,through strategies based on the physiological characteristics of the BBB and other modifications,including enhancement of BBB permeability,nanotechnology can offer a solution to this problem and facilitate drug delivery across the BBB.Although nanomaterials are often used as carriers for PD treatment,their biological activity is ignored.Several studies in recent years have shown that nanomaterials can improve PD symptoms via their own nano-bio effects.In this review,we first summarize the physiological features of the BBB and then discuss the design of appropriate brain-targeted delivery nanoplatforms for PD treatment.Subsequently,we highlight the emerging strategies for crossing the BBB and the development of novel nanomaterials with anti-PD nano-biological effects.Finally,we discuss the current challenges in nanomaterial-based PD treatment and the future trends in this field.Our review emphasizes the clinical value of nanotechnology in PD treatment based on recent patents and could guide researchers working in this area in the future.展开更多
Sports matches are very popular all over the world.The prediction of a sports match is helpful to grasp the team's state in time and adjust the strategy in the process of the match.It's a challenging effort to...Sports matches are very popular all over the world.The prediction of a sports match is helpful to grasp the team's state in time and adjust the strategy in the process of the match.It's a challenging effort to predict a sports match.Therefore,a method is proposed to predict the result of the next match by using teams'historical match data.We combined the Long Short-Term Memory(LSTM)model with the attention mechanism and put forward an ASLSTM model for predicting match results.Furthermore,to ensure the timeliness of the prediction,we add the time sliding window to make the prediction have better timeliness.Taking the football match as an example,we carried out a case study and proposed the feasibility of this method.展开更多
Edge-computing-enabled smart greenhouses are a representative application of the Internet of Things(IoT)technology,which can monitor the environmental information in real-time and employ the information to contribute ...Edge-computing-enabled smart greenhouses are a representative application of the Internet of Things(IoT)technology,which can monitor the environmental information in real-time and employ the information to contribute to intelligent decision-making.In the process,anomaly detection for wireless sensor data plays an important role.However,the traditional anomaly detection algorithms originally designed for anomaly detection in static data do not properly consider the inherent characteristics of the data stream produced by wireless sensors such as infiniteness,correlations,and concept drift,which may pose a considerable challenge to anomaly detection based on data stream and lead to low detection accuracy and efficiency.First,the data stream is usually generated quickly,which means that the data stream is infinite and enormous.Hence,any traditional off-line anomaly detection algorithm that attempts to store the whole dataset or to scan the dataset multiple times for anomaly detection will run out of memory space.Second,there exist correlations among different data streams,and traditional algorithms hardly consider these correlations.Third,the underlying data generation process or distribution may change over time.Thus,traditional anomaly detection algorithms with no model update will lose their effects.Considering these issues,a novel method(called DLSHiForest)based on Locality-Sensitive Hashing and the time window technique is proposed to solve these problems while achieving accurate and efficient detection.Comprehensive experiments are executed using a real-world agricultural greenhouse dataset to demonstrate the feasibility of our approach.Experimental results show that our proposal is practical for addressing the challenges of traditional anomaly detection while ensuring accuracy and efficiency.展开更多
The Late Paleozoic Taiyuan Formation in North China is mainly composed of a mixed shallow-marine carbonate and terrigenous clastic deposits.Its basal limestones have been constrained in the late Pennsylvanian to Early...The Late Paleozoic Taiyuan Formation in North China is mainly composed of a mixed shallow-marine carbonate and terrigenous clastic deposits.Its basal limestones have been constrained in the late Pennsylvanian to Early Permian.To further constrain the age of the lowest Taiyuan Formation,we obtained two genera and 16 species of conodonts from the bottom limestones of the Taiyuan Formation in two sections in Henan Province,southern North China.The fauna includes Idiognathodus hebeiensis,Streptognathodus isolatus,S.elongatus,S.cf.longus,S.acuminatus,S.cf.recreatus,S.cf.cristellaris,S.bellus,S.invaginatus,S.wabaunsensis,S.glenisteri,S.conjunctus,S.binodosus,S.fuchengensis,S.nodulinearis,and S.sp.A genus of Streptognathodus dominated conodont assemblage with some Idiognathodus elements.It indicates a late Gzhelian(latest Pennsylvanian)age for the lowest Taiyuan Formation in southern North China.This is consistent with recently published high-precision zircon U-Pb ages from ash layers.Based on conodont biostratigraphy,the basal limestones of the Taiyuan Formation in Henan Province(southern North China)can be correlated with the upper part of Miaogou limestones of the Taiyuan Formation in Shanxi Province(northern North China).This correlation might reflect a significant sea-level rise in North China,possibly corresponding to a deglaciation event at the Permo-Carboniferous transition.展开更多
The demand for autonomous motion control of unmanned aerial vehicles in air combat is boosted as taking the initiative in combat appears more and more crucial.Unmanned aerial vehicles inability to manoeuvre autonomous...The demand for autonomous motion control of unmanned aerial vehicles in air combat is boosted as taking the initiative in combat appears more and more crucial.Unmanned aerial vehicles inability to manoeuvre autonomously during air combat that features highly dynamic and uncertain manoeuvres of the enemy;however,limits their combat capabilities,which proves to be very challenging.To meet the challenge,this article proposes an autonomous manoeuvre decision model using an expert actor-based soft actor critic algorithm that reconstructs empirical replay buffer with expert experience.Specifically,the algorithm uses a small amount of expert experience to increase the diversity of the samples,which can largely improve the exploration and utilisation efficiency of deep reinforcement learning.And to simulate the complex battlefield environment,a one-toone air combat model is established and the concept of missile's attack region is introduced.The model enables the one-to-one air combat to be simulated under different initial battlefield situations.Simulation results show that the expert actor-based soft actor critic algorithm can find the most favourable policy for unmanned aerial vehicles to defeat the opponent faster,and converge more quickly,compared with the soft actor critic algorithm.展开更多
In order to solve the springback problem in sheet metal forming, the trial and error method is a widely used method in the factory, which is time-consuming and costly for its non-direction and non-quantitative. Finite...In order to solve the springback problem in sheet metal forming, the trial and error method is a widely used method in the factory, which is time-consuming and costly for its non-direction and non-quantitative. Finite element simulation is an e ective method to predict the springback of complex shape parts, but its precision is sensitive to the simulation model, particularly material model and boundary conditions. In this paper, the simple iterative method is introduced to establish the iterative compensation algorithm, and the convergence criterion of iterative parameters is put forward. In addition, the new algorithm is applied to the V-free bending and stretch-bending processes, and the convergence of curvature and bending angle is proved theoretically and verified experimentally. At the same time,the iterative compensation experiments for plane bending show that, the new method can predict the next compensaantido tnh ev atlaureg ebta cseurdv oatnu trhe ew sitphri tnhgeb earcrko ro fo fe laecshs ttehsat,n s0 o. 5 th%a ta rteh eo btatraigneet db aefntedri n2 g-3 a nitgelrea tiwoitnhs.t Thhei se rrreosre aorf clhe sps rtohpaons e±s 0 a.1%new iterative compensation algorithm to predict springback in sheet metal forming process, where each compensation value depends only on the iteration parameter di erence before and after springback for the same forming process of same material.展开更多
BACKGROUND Signet-ring cell carcinoma(SRCC)was previously thought to have a worse prognosis than other differentiated gastric cancer(GC),however,recent studies have shown that the prognosis of SRCC is related to patho...BACKGROUND Signet-ring cell carcinoma(SRCC)was previously thought to have a worse prognosis than other differentiated gastric cancer(GC),however,recent studies have shown that the prognosis of SRCC is related to pathological type.We hypothesize that patients with SRCC and with different SRCC pathological components have different probability of lymph node metastasis(LNM).AIM To establish models to predict LNM in early GC(EGC),including early gastric SRCC.METHODS Clinical data from EGC patients who had undergone gastrectomy at the First Affiliated Hospital of Nanjing Medical University from January 2012 to March 2022 were reviewed.The patients were divided into three groups based on type:Pure SRCC,mixed SRCC,and non-signet ring cell carcinoma(NSRC).The risk factors were identified through statistical tests using SPSS 23.0,R,and EmpowerStats software.RESULTS A total of 1922 subjects with EGC were enrolled in this study,and included 249 SRCC patients and 1673 NSRC patients,while 278 of the patients(14.46%)presented with LNM.Multivariable analysis showed that gender,tumor size,depth of invasion,lymphovascular invasion,ulceration,and histological subtype were independent risk factors for LNM in EGC.Establishment and analysis using prediction models of EGC showed that the artificial neural network model was better than the logistic regression model in terms of sensitivity and accuracy(98.0%vs 58.1%,P=0.034;88.4%vs 86.8%,P<0.001,respectively).Among the 249 SRCC patients,LNM was more common in mixed(35.06%)rather than in pure SRCC(8.42%,P<0.001).The area under the ROC curve of the logistic regression model for LNM in SRCC was 0.760(95%CI:0.682-0.843),while the area under the operating characteristic curve of the internal validation set was 0.734(95%CI:0.643-0.826).The subgroups analysis of pure types showed that LNM was more common in patients with a tumor size>2 cm(OR=5.422,P=0.038).CONCLUSION A validated prediction model was developed to recognize the risk of LNM in EGC and early gastric SRCC,which can aid in pre-surgical decision making of the best method of treatment for patients.展开更多
This article proposes a novel method for maintaining the trajectory of an aerial manipulator by utilizing a fast nonsingular terminal sliding mode(FNTSM)manifold and a linear extended state observer(LESO).The develope...This article proposes a novel method for maintaining the trajectory of an aerial manipulator by utilizing a fast nonsingular terminal sliding mode(FNTSM)manifold and a linear extended state observer(LESO).The developed controlmethod applies an FNTSMto ensure the tracking performance’s control accuracy,and an LESO to estimate the system’s unmodeled dynamics and external disturbances.Additionally,an improved salp swarm algorithm(ISSA)is employed to parameter tune the suggested controller by integrating the salp swarmtechnique with a cloud model.This approach also uses a model-free scheme to reduce the complexity of controller design without relying on complex and precise dynamics models.The simulation results show that the proposed controller outperforms linear active rejection disturbance control and PID controllers in terms of transient performance and resilience against lumped disturbances,and the ISSA can help the proposed controller find optimal control parameters.展开更多
With the increasing demand for interactive aerial operations,the application of aerial manipulators is becoming more promising.However,there are a few critical problems on how to improve the energetic efficiency and p...With the increasing demand for interactive aerial operations,the application of aerial manipulators is becoming more promising.However,there are a few critical problems on how to improve the energetic efficiency and pose control of the aerialmanipulator forpractical application.In this paper,a novel cable-drivenaerialmanipulatorused for remote water sampling is proposed and then its rigid-flexible coupling dynamics model is constructed which takes joint flexibility into account.To achieve high precision joint position tracking under lumped disturbances,a newly controller,which consists of three parts:linear extended state observer,adaptive super-twisting strategy,and fractional-order nonsingular terminal sliding mode control,is proposed.The linear extended state observer is adopted to approximate unmeasured states and unknown lumped disturbances and achieve model-free control structure.The adaptive super-twisting strategy and fractional-order nonsingular terminal sliding mode control are combined together to achieve good control performance and counteract chattering problem.The Lyapunovmethod is utilized to prove the overall stability and convergence of the system.Lastly,various visualization simulations and ground experiments are conducted,verifying the effectiveness of our strategy,and all outcomes demonstrate its superiorities over the existing control strategies.展开更多
Machine learning is a novel and powerful technology and has been widely used in various science topics.We demonstrate a machine-learning-based approach built by a set of general metrics and rules inspired by physics.T...Machine learning is a novel and powerful technology and has been widely used in various science topics.We demonstrate a machine-learning-based approach built by a set of general metrics and rules inspired by physics.Taking advantages of physical constraints,such as dimension identity,symmetry and generalization,we succeed to approach the Gell-Mann-Okubo formula using a technique of symbolic regression.This approach can effectively find explicit solutions among user-defined observables,and can be extensively applied to studying exotic hadron spectrum.展开更多
Alzheimer’s disease(AD)is a complex neurodegenerative condition.5α-epoxyalantolactone(5α-EAL),a eudesmane-type sesquiterpene isolated from the herb of Inula macrophylla,has various pharmacological effects.This work...Alzheimer’s disease(AD)is a complex neurodegenerative condition.5α-epoxyalantolactone(5α-EAL),a eudesmane-type sesquiterpene isolated from the herb of Inula macrophylla,has various pharmacological effects.This work supposed to investigate the improved impact of 5α-EAL on cognitive impairment.5α-EAL inhibited the generation of nitric oxide(NO)in BV-2 cells stimulated with lipopolysaccharide(LPS)with an EC50 of 6.2μM.5α-EAL significantly reduced the production of prostaglandin E2(PGE2)and tumor necrosis factor-α(TNF-α),while also inhibiting the production of cyclooxygenase-2(COX-2)and inducible nitric oxide synthase(iNOS)proteins.The ability of 5α-EAL to penetrate the blood-brain barrier(BBB)was confirmed via a parallel artificial membrane permeation assay.Scopolamine(SCOP)-induced AD mice model was employed to assess the improved impacts of 5α-EAL on cognitive impairment in vivo.After the mice were pretreated with 5α-EAL(10 and 30 mg/kg per day,i.p.)for 21 days,the behavioral experiments indicated that the administration of the 5α-EAL could alleviate the cognitive and memory impairments.5α-EAL significantly reduced the AChE activity in the brain of SCOP-induced AD mice.In summary,these findings highlight the beneficial effects of the natural product 5α-EAL as a potential bioactive compound for attenuating cognitive deficits in AD due to its pharmacological profile.展开更多
Hair cell regeneration is the fundamental method of correcting hearing loss and balance disorders caused by hair cell damage or loss. How to promote hair cell regeneration is a hot focus in current research. In mammal...Hair cell regeneration is the fundamental method of correcting hearing loss and balance disorders caused by hair cell damage or loss. How to promote hair cell regeneration is a hot focus in current research. In mammals, cochlear hair cells cannot be regenerated and few vestibular hair cells can be renewed through spontaneous regeneration. However, Math1 gene transfer allows a few inner ear cells to be transformed into hair cells in vitro or in vivo. Hair cells can be renewed through two possible means in birds： supporting cell differentiation and transdifferentiation with or without cell division. Hair cell regeneration is strongly associated with cell proliferation. Therefore, this study explored the relationship between Math1-induced vestibular hair cell regeneration and cell division in mammals. The mouse vestibule was isolated to harvest vestibular epithelial cells. Ad-Math1-enhanced green fluorescent protein （EGFP） was used to track cell division during hair cell transformation.5-Bromo-2′-deoxyuridine （BrdU） was added to track cell proliferation at various time points. Immunocytochemistry was utilized to determine cell differentiation and proliferation. Results demonstrated that when epithelial cells were in a higher proliferative stage, more of these cells differentiated into hair cells by Math1 gene transfer. However, in the low proliferation stage, no BrdU-positive cells were seen after Math1 gene transfer. Cell division always occurred before Math1 transfection but not during or after Math1 transfection, when cells were labeled with BrdU before and after Ad-Math1-EGFP transfection. These results confirm that vestibular epithelial cells with high proliferative potential can differentiate into new hair cells by Math1 gene transfer, but this process is independent of cell proliferation.展开更多
BACKGROUND Venous thrombosis(VT) is one of the minor complications of pacemaker lead extraction. It is often found due to the swelling of the limbs after the extraction. It is easy to be neglected or even misdiagnosed...BACKGROUND Venous thrombosis(VT) is one of the minor complications of pacemaker lead extraction. It is often found due to the swelling of the limbs after the extraction. It is easy to be neglected or even misdiagnosed in the absence of typical clinical symptoms. The incidence, risk factors, and long-term impact of this complication are still unclear. Herein, we report a case of deep VT caused by transvenous lead extraction, which is easily misdiagnosed.CASE SUMMARY A 66-year-old woman underwent a pacemaker lead extraction at our hospital because of a pacemaker pocket infection. After the extraction, she began to experience intermittent fever accompanied by sweating. The highest body temperature recorded was 37.9℃. Additionally, she reported migratory pain that made her uncomfortable. The pain was mistakenly thought to be caused by operation trauma. At first, the pain radiated from the left chest to the mandible.Then, the pain in the left chest was alleviated, but pain in the left neck and throat appeared. Finally, the pain was confined to the mandible and a submandibular mass was palpated with no other abnormalities upon physical examination.Computed tomography venography and angiography finally indicated that the fever and pain were the symptoms of thrombophlebitis caused by lead extraction.The patient was then treated with rivaroxaban for more than three months and has shown no symptoms since she left the hospital.CONCLUSION The possibility of thrombosis should be considered when pain and recurrent fever occur after pacemaker lead extraction.展开更多
The precise estimation of the frequency of the signal is of great significance in the Radar system, the electronic warfare system and many other systems. In this paper, we propose a development and verification platfo...The precise estimation of the frequency of the signal is of great significance in the Radar system, the electronic warfare system and many other systems. In this paper, we propose a development and verification platform for the frequency estimation system in the Matlab and Simulink environment. Its open-extensibility architecture enables the performance evaluation of different frequency estimation algorithms and its graphic interface can greatly promote the system design, simulation and verification efficiency.展开更多
[Objectives]To study the effect and mechanism of baicalin on the activation of NLRP3 inflammasome in human fibroblast like synoviocytes of rheumatoid arthritis(HFLS-RA).[Methods]To confirm that baicalin alleviated the...[Objectives]To study the effect and mechanism of baicalin on the activation of NLRP3 inflammasome in human fibroblast like synoviocytes of rheumatoid arthritis(HFLS-RA).[Methods]To confirm that baicalin alleviated the activation of NLRP3 inflammasome in HFLS-RA,the expression of NLRP3 before and after baicalin treatment was observed by immunofluorescence.Western blot was used to detect the protein expression of p-PI3K,p-Akt,NF-κB p65,NLRP3,ASC and caspase-1 after baicalin treatment for 48 h,and the contents of IL-1 and IL-18 in the supernatents were detected by ELISA.In order to explore the mechanism of baicalin alleviating the activation of NLRP3 inflammasome,the corresponding relationship between let-7i-3p and PIK3CA was verified by double luciferin and Westen blot analysis.The expression of let-7i-3p and PI3K before and after baicalin intervention was detected by RT-qPCR.let-7i-3p interference was used to verify whether baicalin mitigated the activation of enhanced NLRP3 inflammasome.[Results]Baicalin(50 and 100 mg/L)significantly reduced the activation of NLRP3 inflammasome,inhibited the protein expressions of p-PI3K,p-Akt,NF-κB p65,NLRP3,ASC and caspase-1,and the secretion of IL-1 and IL-18.let-7i-3p and PIK3CA had a targeted correspondence,and baicalin up-regulated the expression of let-7i-3p and down-regulated the expression of PIK3CA.Baicalin attenuated the activation of NLRP3 inflammasome enhanced by let-7i-3p interference.[Conclusions]Baicalin can up-regulate let-7i-3p expression,inhibit PI3K/Akt/NF-κB signal transduction,and thus reduce the activation of NLRP3 inflammasome in HFLS-RA.展开更多
文摘BACKGROUND Endometriosis affects approximately 10%of reproductive-age women and is frequently associated with chronic pelvic pain.Patients with endometriosis often experience comorbid depression and anxiety,but the underlying mechanisms connecting these conditions are unclear.AIM To assess the prevalence of depression and anxiety in endometriosis patients and explore neuroimmune mechanisms mediated via inflammatory biomarkers.METHODS A retrospective cohort study was conducted with 200 patients with endometriosis-associated chronic pain from June 2020 to December 2024.Depression and anxiety were assessed using validated psychological instruments.Inflammation biomarkers interleukin(ILs)(IL-6,IL-1β),tumor necrosis factor-alpha,C-reactive protein,and brain-derived neurotrophic factor were measured in serum.Pain severity was assessed using visual analog scales.Correlation and regression analyses were performed to examine relationships between inflammatory markers,pain severity,and psychological outcomes.RESULTS Among the 200 patients,42.5%exhibited clinically significant depression and 51.0%showed anxiety symptoms.Serum levels of IL-6,IL-1β,tumor necrosis factor-alpha,and C-reactive protein were significantly higher in patients with comorbid depression and anxiety compared with those without psychological symptoms(P<0.001).Brainderived neurotrophic factor levels were lower in the depression group.Pain severity positively correlated with inflammatory marker levels and with depression and anxiety scores.CONCLUSION Overall,the findings suggest that inflammatory factors mediate a neuroimmune mechanism linking endometriosisassociated chronic pain with depression and anxiety.Therapeutic targets for managing psychological comorbidities in patients with endometriosis through anti-inflammatory interventions should be explored,and an integrated treatment approach addressing both physical and psychological symptoms is emphasized.
基金This study was approved by the Medical Ethics Committee of Jieshou City People's Hospital,approval No.[2022]21.
文摘BACKGROUND Currently,only tumors classified as LR-5 are considered definitive hepatocellular carcinoma(HCC),and no further pathologic confirmation is required to initiate therapy.Previous studies have shown that the sensitivity of LR-5 is modest,and lesions enhanced by gadoxetic acid(Gd-EOB-DTPA)may exhibit lower sensitivity than those enhanced by Gd-DTPA.AIM To identify malignant ancillary features(AFs)that can independently and significantly predict HCC in Liver Imaging Reporting and Data System version 2018,and to develop modified LR-5 criteria to improve diagnostic performance on Gd-EOB-DTPA-enhanced magnetic resonance imaging.METHODS Imaging data from patients with HCC risk factors who underwent abdominal Gd-EOB-DTPA-enhanced magnetic resonance imaging were collected.Univariate and multivariate logistic regression analyses were performed to determine AFs that could independently and significantly predict HCC.The modified LR-5 criteria involved reclassifying LR-4/LR-3 lesions based on major features combined with independently significant AFs for HCC,or by substituting threshold growth with significant AFs.McNemar's test was used to compare the diagnostic performance of the modified LR-5 criteria.RESULTS A total of 244 lesions from 216 patients were included.Transitional phase hypointensity,mild-moderate T2 hyperintensity,and fat in mass(more than adjacent liver)were identified as significant independent predictors of HCC.Using the modified LR-5 criteria(e.g.,LR-5-M1:LR-4+transitional phase hypointensity;LR-5-M4:LR-5 by transitional phase hypointensity instead of threshold growth;LR-5-M5:LR-5 by mild-moderate T2 hyperintensity instead of threshold growth;LR-5-M8:LR-3/LR-4+any two features of transitional phase hypointensity/mild-moderate T2 hyperintensity/fat in mass),sensitivities were significantly increased(88.5%-89.1%)compared to the standard LR-5(60.6%;all P values<0.05),while specificities(84.8%-89.9%)remained largely unchanged(93.7%;all P values>0.05).The LR-5-M8 criterion achieved the highest sensitivity.CONCLUSION Mild-moderate T2 hyperintensity,transitional phase hypointensity,and fat in mass are independent and significant predictors of HCC malignant AFs.The modified LR-5 criteria can improve sensitivity without significantly reducing specificity.
基金supported in part by the National Nat-ural Science Foundation of China(No.51977012,No.52307080).
文摘This study proposes a method for analyzing the security distance of an Active Distribution Network(ADN)by incorporating the demand response of an Energy Hub(EH).Taking into account the impact of stochastic wind-solar power and flexible loads on the EH,an interactive power model was developed to represent the EH’s operation under these influences.Additionally,an ADN security distance model,integrating an EH with flexible loads,was constructed to evaluate the effect of flexible load variations on the ADN’s security distance.By considering scenarios such as air conditioning(AC)load reduction and base station(BS)load transfer,the security distances of phases A,B,and C increased by 17.1%,17.2%,and 17.7%,respectively.Furthermore,a multi-objective optimal power flow model was formulated and solved using the Forward-Backward Power Flow Algorithm,the NSGA-II multi-objective optimization algo-rithm,and the maximum satisfaction method.The simulation results of the IEEE33 node system example demonstrate that after opti-mization,the total energy cost for one day is reduced by 0.026%,and the total security distance limit of the ADN’s three phases is improved by 0.1 MVA.This method effectively enhances the security distance,facilitates BS load transfer and AC load reduction,and contributes to the energy-saving,economical,and safe operation of the power system.
基金support from the the National Natural Science Foundation of China(Grant Nos.12474199(RM)and 12374213(YC))Fundamental Research Funds for Central Universities of China(Grant No.20720240144(RM))111 Project(Grant No.B16029).
文摘Vesicles of lipid bilayer can adopt a variety of shapes due to different coating proteins.The ability of proteins to reshape membrane is typically characterized by inducing spontaneous curvature of the membrane at the coated area.BAR family proteins are known to have a crescent shape and can induce membrane curvature along their concaved body axis but not in the perpendicular direction.We model this type of proteins as a rod-shaped molecule with an orientation and induce normal curvature along its orientation in the tangential plane of the membrane surface.We show how a ring of these proteins reshapes an axisymmetric vesicle when the protein curvature or orientation is varied.A discontinuous shape transformation from a protrusion shape without a neck to a one with a neck is found.Increasing the rigidity of the protein ring is able to smooth out the transition.Furthermore,we show that varying the protein orientation is able to induce an hourglass-shaped neck,which is significantly narrower than the reciprocal of the protein curvature.Our results offer a new angle to rationalize the helical structure formed by many proteins that carry out membrane fission functions.
基金supported by the National Natural Science Foundation of China(32160128).
文摘new species in the genus Amauromyza Hendel,1931,Amauromyza(Cephalomyza)wuyuanensis Fu&Shi sp.nov.,is described and illustrated.A.(Cephalomyza)karli(Hendel,1927)is newly discovered from China.So far,five species in the genus Amauromyza have been reported from China.A key to these species and a checklist with host plants and distributions are presented.
基金This work was financially supported through grants from the Guangdong Basic and Applied Basic Research Foundation(2019B1515120043)National Natural Science Foundation of China(File No.82104354)+2 种基金the Science and Technology Development Fund,Macao SAR(File No.0016/2021/A)and the Open Project of Key Laboratory of Modern Preparation of Traditional Chinese Medicine,Ministry of Education,Jiangxi University of Chinese Medicine(zdsys-202101)Open access funding provided by Shanghai Jiao Tong University
文摘Parkinson’s disease(PD),a neurodegenerative disease that shows a high incidence in older individuals,is becoming increasingly prevalent.Unfortunately,there is no clinical cure for PD,and novel anti-PD drugs are therefore urgently required.However,the selective permeability of the blood–brain barrier(BBB)poses a huge challenge in the development of such drugs.Fortunately,through strategies based on the physiological characteristics of the BBB and other modifications,including enhancement of BBB permeability,nanotechnology can offer a solution to this problem and facilitate drug delivery across the BBB.Although nanomaterials are often used as carriers for PD treatment,their biological activity is ignored.Several studies in recent years have shown that nanomaterials can improve PD symptoms via their own nano-bio effects.In this review,we first summarize the physiological features of the BBB and then discuss the design of appropriate brain-targeted delivery nanoplatforms for PD treatment.Subsequently,we highlight the emerging strategies for crossing the BBB and the development of novel nanomaterials with anti-PD nano-biological effects.Finally,we discuss the current challenges in nanomaterial-based PD treatment and the future trends in this field.Our review emphasizes the clinical value of nanotechnology in PD treatment based on recent patents and could guide researchers working in this area in the future.
文摘Sports matches are very popular all over the world.The prediction of a sports match is helpful to grasp the team's state in time and adjust the strategy in the process of the match.It's a challenging effort to predict a sports match.Therefore,a method is proposed to predict the result of the next match by using teams'historical match data.We combined the Long Short-Term Memory(LSTM)model with the attention mechanism and put forward an ASLSTM model for predicting match results.Furthermore,to ensure the timeliness of the prediction,we add the time sliding window to make the prediction have better timeliness.Taking the football match as an example,we carried out a case study and proposed the feasibility of this method.
基金supported in part by the Fundamental Research Funds for the Central Universities under Grant No.30919011282.
文摘Edge-computing-enabled smart greenhouses are a representative application of the Internet of Things(IoT)technology,which can monitor the environmental information in real-time and employ the information to contribute to intelligent decision-making.In the process,anomaly detection for wireless sensor data plays an important role.However,the traditional anomaly detection algorithms originally designed for anomaly detection in static data do not properly consider the inherent characteristics of the data stream produced by wireless sensors such as infiniteness,correlations,and concept drift,which may pose a considerable challenge to anomaly detection based on data stream and lead to low detection accuracy and efficiency.First,the data stream is usually generated quickly,which means that the data stream is infinite and enormous.Hence,any traditional off-line anomaly detection algorithm that attempts to store the whole dataset or to scan the dataset multiple times for anomaly detection will run out of memory space.Second,there exist correlations among different data streams,and traditional algorithms hardly consider these correlations.Third,the underlying data generation process or distribution may change over time.Thus,traditional anomaly detection algorithms with no model update will lose their effects.Considering these issues,a novel method(called DLSHiForest)based on Locality-Sensitive Hashing and the time window technique is proposed to solve these problems while achieving accurate and efficient detection.Comprehensive experiments are executed using a real-world agricultural greenhouse dataset to demonstrate the feasibility of our approach.Experimental results show that our proposal is practical for addressing the challenges of traditional anomaly detection while ensuring accuracy and efficiency.
基金supported by the National Natural Science Foundation of China(Nos.41572078,41872106,42072013)the BGEG and GPMR laboratory funds(Nos.GKZ18Y660,GPMR201609)。
文摘The Late Paleozoic Taiyuan Formation in North China is mainly composed of a mixed shallow-marine carbonate and terrigenous clastic deposits.Its basal limestones have been constrained in the late Pennsylvanian to Early Permian.To further constrain the age of the lowest Taiyuan Formation,we obtained two genera and 16 species of conodonts from the bottom limestones of the Taiyuan Formation in two sections in Henan Province,southern North China.The fauna includes Idiognathodus hebeiensis,Streptognathodus isolatus,S.elongatus,S.cf.longus,S.acuminatus,S.cf.recreatus,S.cf.cristellaris,S.bellus,S.invaginatus,S.wabaunsensis,S.glenisteri,S.conjunctus,S.binodosus,S.fuchengensis,S.nodulinearis,and S.sp.A genus of Streptognathodus dominated conodont assemblage with some Idiognathodus elements.It indicates a late Gzhelian(latest Pennsylvanian)age for the lowest Taiyuan Formation in southern North China.This is consistent with recently published high-precision zircon U-Pb ages from ash layers.Based on conodont biostratigraphy,the basal limestones of the Taiyuan Formation in Henan Province(southern North China)can be correlated with the upper part of Miaogou limestones of the Taiyuan Formation in Shanxi Province(northern North China).This correlation might reflect a significant sea-level rise in North China,possibly corresponding to a deglaciation event at the Permo-Carboniferous transition.
基金acknowledge the National Nature Science Foundation of China(Grant No.62003267)Fundamental Research Funds for the Central Universities(Grant No.G2022KY0602)+1 种基金Technology on Electromagnetic Space Operations and Applications Laboratory(Grant No.2022ZX0090)key core technology research plan of Xi'an(Grant No.21RGZN0016)to provide fund for conducting experiments.
文摘The demand for autonomous motion control of unmanned aerial vehicles in air combat is boosted as taking the initiative in combat appears more and more crucial.Unmanned aerial vehicles inability to manoeuvre autonomously during air combat that features highly dynamic and uncertain manoeuvres of the enemy;however,limits their combat capabilities,which proves to be very challenging.To meet the challenge,this article proposes an autonomous manoeuvre decision model using an expert actor-based soft actor critic algorithm that reconstructs empirical replay buffer with expert experience.Specifically,the algorithm uses a small amount of expert experience to increase the diversity of the samples,which can largely improve the exploration and utilisation efficiency of deep reinforcement learning.And to simulate the complex battlefield environment,a one-toone air combat model is established and the concept of missile's attack region is introduced.The model enables the one-to-one air combat to be simulated under different initial battlefield situations.Simulation results show that the expert actor-based soft actor critic algorithm can find the most favourable policy for unmanned aerial vehicles to defeat the opponent faster,and converge more quickly,compared with the soft actor critic algorithm.
基金Supported by Hebei Provincial Natural Science Foundation of in China(Grant Nos.E2015203244,E2016203266)Program for the Youth Top-notch Talents of Hebei Province
文摘In order to solve the springback problem in sheet metal forming, the trial and error method is a widely used method in the factory, which is time-consuming and costly for its non-direction and non-quantitative. Finite element simulation is an e ective method to predict the springback of complex shape parts, but its precision is sensitive to the simulation model, particularly material model and boundary conditions. In this paper, the simple iterative method is introduced to establish the iterative compensation algorithm, and the convergence criterion of iterative parameters is put forward. In addition, the new algorithm is applied to the V-free bending and stretch-bending processes, and the convergence of curvature and bending angle is proved theoretically and verified experimentally. At the same time,the iterative compensation experiments for plane bending show that, the new method can predict the next compensaantido tnh ev atlaureg ebta cseurdv oatnu trhe ew sitphri tnhgeb earcrko ro fo fe laecshs ttehsat,n s0 o. 5 th%a ta rteh eo btatraigneet db aefntedri n2 g-3 a nitgelrea tiwoitnhs.t Thhei se rrreosre aorf clhe sps rtohpaons e±s 0 a.1%new iterative compensation algorithm to predict springback in sheet metal forming process, where each compensation value depends only on the iteration parameter di erence before and after springback for the same forming process of same material.
基金National Natural Science Foundation of China,No.82200625 and No.82100595Youth Talent Development Program,No.YNRCQN0313+2 种基金Young Scholar Fostering Fund of the First Affiliated Hospital of Nanjing Medical University,No.PY2021023Top Talent of Changzhou“The 14th Five-Year Plan”High-Level Health Talents Training Project,No.2022CZBJ051Natural Science Foundation of Jiangsu Province,China,No.BK20210958.
文摘BACKGROUND Signet-ring cell carcinoma(SRCC)was previously thought to have a worse prognosis than other differentiated gastric cancer(GC),however,recent studies have shown that the prognosis of SRCC is related to pathological type.We hypothesize that patients with SRCC and with different SRCC pathological components have different probability of lymph node metastasis(LNM).AIM To establish models to predict LNM in early GC(EGC),including early gastric SRCC.METHODS Clinical data from EGC patients who had undergone gastrectomy at the First Affiliated Hospital of Nanjing Medical University from January 2012 to March 2022 were reviewed.The patients were divided into three groups based on type:Pure SRCC,mixed SRCC,and non-signet ring cell carcinoma(NSRC).The risk factors were identified through statistical tests using SPSS 23.0,R,and EmpowerStats software.RESULTS A total of 1922 subjects with EGC were enrolled in this study,and included 249 SRCC patients and 1673 NSRC patients,while 278 of the patients(14.46%)presented with LNM.Multivariable analysis showed that gender,tumor size,depth of invasion,lymphovascular invasion,ulceration,and histological subtype were independent risk factors for LNM in EGC.Establishment and analysis using prediction models of EGC showed that the artificial neural network model was better than the logistic regression model in terms of sensitivity and accuracy(98.0%vs 58.1%,P=0.034;88.4%vs 86.8%,P<0.001,respectively).Among the 249 SRCC patients,LNM was more common in mixed(35.06%)rather than in pure SRCC(8.42%,P<0.001).The area under the ROC curve of the logistic regression model for LNM in SRCC was 0.760(95%CI:0.682-0.843),while the area under the operating characteristic curve of the internal validation set was 0.734(95%CI:0.643-0.826).The subgroups analysis of pure types showed that LNM was more common in patients with a tumor size>2 cm(OR=5.422,P=0.038).CONCLUSION A validated prediction model was developed to recognize the risk of LNM in EGC and early gastric SRCC,which can aid in pre-surgical decision making of the best method of treatment for patients.
基金supported by the National Natural Science Foundation of China(52005231)Social Development Science and Technology Support Project of Changzhou(CE20215050)Jiangsu Province Graduate Student Practice Innovation Plan(SJCX21_1313,SJCX21_1314).
文摘This article proposes a novel method for maintaining the trajectory of an aerial manipulator by utilizing a fast nonsingular terminal sliding mode(FNTSM)manifold and a linear extended state observer(LESO).The developed controlmethod applies an FNTSMto ensure the tracking performance’s control accuracy,and an LESO to estimate the system’s unmodeled dynamics and external disturbances.Additionally,an improved salp swarm algorithm(ISSA)is employed to parameter tune the suggested controller by integrating the salp swarmtechnique with a cloud model.This approach also uses a model-free scheme to reduce the complexity of controller design without relying on complex and precise dynamics models.The simulation results show that the proposed controller outperforms linear active rejection disturbance control and PID controllers in terms of transient performance and resilience against lumped disturbances,and the ISSA can help the proposed controller find optimal control parameters.
基金supported by the National Natural Science Foundation of China (52005231,52175097)Social Development Science and Technology Support Project of Changzhou (CE20215050).
文摘With the increasing demand for interactive aerial operations,the application of aerial manipulators is becoming more promising.However,there are a few critical problems on how to improve the energetic efficiency and pose control of the aerialmanipulator forpractical application.In this paper,a novel cable-drivenaerialmanipulatorused for remote water sampling is proposed and then its rigid-flexible coupling dynamics model is constructed which takes joint flexibility into account.To achieve high precision joint position tracking under lumped disturbances,a newly controller,which consists of three parts:linear extended state observer,adaptive super-twisting strategy,and fractional-order nonsingular terminal sliding mode control,is proposed.The linear extended state observer is adopted to approximate unmeasured states and unknown lumped disturbances and achieve model-free control structure.The adaptive super-twisting strategy and fractional-order nonsingular terminal sliding mode control are combined together to achieve good control performance and counteract chattering problem.The Lyapunovmethod is utilized to prove the overall stability and convergence of the system.Lastly,various visualization simulations and ground experiments are conducted,verifying the effectiveness of our strategy,and all outcomes demonstrate its superiorities over the existing control strategies.
基金supported by the Guangdong Major Project of Basic and Applied Basic Research(Grant No.2020B0301030008)the National Natural Science Foundation of China(Grant No.12035007)+2 种基金the Guangdong Provincial Fund(Grant No.2019QN01X172)supported by the National Natural Science Foundation of China(Grant No.12070131001)the Deutsche Forschungsgemeinschaft(DFG,German Research Foundation)through the funds provided to the Sino-German Collaborative Research Center TRR110“Symmetries and the Emergence of Structure in QCD”(Grant No.DFG Project-ID 196253076-TRR 110)。
文摘Machine learning is a novel and powerful technology and has been widely used in various science topics.We demonstrate a machine-learning-based approach built by a set of general metrics and rules inspired by physics.Taking advantages of physical constraints,such as dimension identity,symmetry and generalization,we succeed to approach the Gell-Mann-Okubo formula using a technique of symbolic regression.This approach can effectively find explicit solutions among user-defined observables,and can be extensively applied to studying exotic hadron spectrum.
基金supported by the National Key Research and Development Program of China(No.2021YFE0103700)the National Natural Science Foundation of China(U21A20407,22274163,and 22374160)Shaanxi Fundamental Science Research Project for Chemistry and Biology(No.23JHQ054).
文摘Alzheimer’s disease(AD)is a complex neurodegenerative condition.5α-epoxyalantolactone(5α-EAL),a eudesmane-type sesquiterpene isolated from the herb of Inula macrophylla,has various pharmacological effects.This work supposed to investigate the improved impact of 5α-EAL on cognitive impairment.5α-EAL inhibited the generation of nitric oxide(NO)in BV-2 cells stimulated with lipopolysaccharide(LPS)with an EC50 of 6.2μM.5α-EAL significantly reduced the production of prostaglandin E2(PGE2)and tumor necrosis factor-α(TNF-α),while also inhibiting the production of cyclooxygenase-2(COX-2)and inducible nitric oxide synthase(iNOS)proteins.The ability of 5α-EAL to penetrate the blood-brain barrier(BBB)was confirmed via a parallel artificial membrane permeation assay.Scopolamine(SCOP)-induced AD mice model was employed to assess the improved impacts of 5α-EAL on cognitive impairment in vivo.After the mice were pretreated with 5α-EAL(10 and 30 mg/kg per day,i.p.)for 21 days,the behavioral experiments indicated that the administration of the 5α-EAL could alleviate the cognitive and memory impairments.5α-EAL significantly reduced the AChE activity in the brain of SCOP-induced AD mice.In summary,these findings highlight the beneficial effects of the natural product 5α-EAL as a potential bioactive compound for attenuating cognitive deficits in AD due to its pharmacological profile.
基金supported by the National Natural Science Foundation of China(NSFC)grant No.81420108010,81271084 to FLC,81370022,81570920,81000413 to DR,81200740 to JMY,81200738 to NC,81371093 to ZH,81400460 to ZG,81200739 to JW+2 种基金973 Program,grant No.2011CB504500 and 2011CB504506The Innovation Project of Shanghai Municipal Science and Technology Commission,grant No.11411952300 to FLCthe Training Program of the Excellent Young Talents of the Shanghai Municipal Health System,grant No.XYQ2013084 to DR
文摘Hair cell regeneration is the fundamental method of correcting hearing loss and balance disorders caused by hair cell damage or loss. How to promote hair cell regeneration is a hot focus in current research. In mammals, cochlear hair cells cannot be regenerated and few vestibular hair cells can be renewed through spontaneous regeneration. However, Math1 gene transfer allows a few inner ear cells to be transformed into hair cells in vitro or in vivo. Hair cells can be renewed through two possible means in birds： supporting cell differentiation and transdifferentiation with or without cell division. Hair cell regeneration is strongly associated with cell proliferation. Therefore, this study explored the relationship between Math1-induced vestibular hair cell regeneration and cell division in mammals. The mouse vestibule was isolated to harvest vestibular epithelial cells. Ad-Math1-enhanced green fluorescent protein （EGFP） was used to track cell division during hair cell transformation.5-Bromo-2′-deoxyuridine （BrdU） was added to track cell proliferation at various time points. Immunocytochemistry was utilized to determine cell differentiation and proliferation. Results demonstrated that when epithelial cells were in a higher proliferative stage, more of these cells differentiated into hair cells by Math1 gene transfer. However, in the low proliferation stage, no BrdU-positive cells were seen after Math1 gene transfer. Cell division always occurred before Math1 transfection but not during or after Math1 transfection, when cells were labeled with BrdU before and after Ad-Math1-EGFP transfection. These results confirm that vestibular epithelial cells with high proliferative potential can differentiate into new hair cells by Math1 gene transfer, but this process is independent of cell proliferation.
基金Supported by Nanjing Foundation for Development of Science and Technology,No.ZKX14018
文摘BACKGROUND Venous thrombosis(VT) is one of the minor complications of pacemaker lead extraction. It is often found due to the swelling of the limbs after the extraction. It is easy to be neglected or even misdiagnosed in the absence of typical clinical symptoms. The incidence, risk factors, and long-term impact of this complication are still unclear. Herein, we report a case of deep VT caused by transvenous lead extraction, which is easily misdiagnosed.CASE SUMMARY A 66-year-old woman underwent a pacemaker lead extraction at our hospital because of a pacemaker pocket infection. After the extraction, she began to experience intermittent fever accompanied by sweating. The highest body temperature recorded was 37.9℃. Additionally, she reported migratory pain that made her uncomfortable. The pain was mistakenly thought to be caused by operation trauma. At first, the pain radiated from the left chest to the mandible.Then, the pain in the left chest was alleviated, but pain in the left neck and throat appeared. Finally, the pain was confined to the mandible and a submandibular mass was palpated with no other abnormalities upon physical examination.Computed tomography venography and angiography finally indicated that the fever and pain were the symptoms of thrombophlebitis caused by lead extraction.The patient was then treated with rivaroxaban for more than three months and has shown no symptoms since she left the hospital.CONCLUSION The possibility of thrombosis should be considered when pain and recurrent fever occur after pacemaker lead extraction.
文摘The precise estimation of the frequency of the signal is of great significance in the Radar system, the electronic warfare system and many other systems. In this paper, we propose a development and verification platform for the frequency estimation system in the Matlab and Simulink environment. Its open-extensibility architecture enables the performance evaluation of different frequency estimation algorithms and its graphic interface can greatly promote the system design, simulation and verification efficiency.
基金Supported by the National Natural Science Foundation of China(82360802):the Natural Science Foundation of Ningxia Province,China(2022AAC 03152).
文摘[Objectives]To study the effect and mechanism of baicalin on the activation of NLRP3 inflammasome in human fibroblast like synoviocytes of rheumatoid arthritis(HFLS-RA).[Methods]To confirm that baicalin alleviated the activation of NLRP3 inflammasome in HFLS-RA,the expression of NLRP3 before and after baicalin treatment was observed by immunofluorescence.Western blot was used to detect the protein expression of p-PI3K,p-Akt,NF-κB p65,NLRP3,ASC and caspase-1 after baicalin treatment for 48 h,and the contents of IL-1 and IL-18 in the supernatents were detected by ELISA.In order to explore the mechanism of baicalin alleviating the activation of NLRP3 inflammasome,the corresponding relationship between let-7i-3p and PIK3CA was verified by double luciferin and Westen blot analysis.The expression of let-7i-3p and PI3K before and after baicalin intervention was detected by RT-qPCR.let-7i-3p interference was used to verify whether baicalin mitigated the activation of enhanced NLRP3 inflammasome.[Results]Baicalin(50 and 100 mg/L)significantly reduced the activation of NLRP3 inflammasome,inhibited the protein expressions of p-PI3K,p-Akt,NF-κB p65,NLRP3,ASC and caspase-1,and the secretion of IL-1 and IL-18.let-7i-3p and PIK3CA had a targeted correspondence,and baicalin up-regulated the expression of let-7i-3p and down-regulated the expression of PIK3CA.Baicalin attenuated the activation of NLRP3 inflammasome enhanced by let-7i-3p interference.[Conclusions]Baicalin can up-regulate let-7i-3p expression,inhibit PI3K/Akt/NF-κB signal transduction,and thus reduce the activation of NLRP3 inflammasome in HFLS-RA.
