The strain transfer behavior of graphene and black phosphorus heterostructure on fexible substrates plays a crucial role in the functionality and regulation of the device.Specifically,it is imperative to investigate t...The strain transfer behavior of graphene and black phosphorus heterostructure on fexible substrates plays a crucial role in the functionality and regulation of the device.Specifically,it is imperative to investigate the anisotropy associated with strain transfer at the black phosphorus interface.In this study,a sample transfer method was proposed to prevent the contact of black phosphorus with water,achieving monolayer graphene and few-layer black phosphorus heterostructures on a PET flm substrate.Micro-Raman spectroscopy was used to measure the strain of graphene and black phosphorus when the PET flm substrate was under uniaxial tensile loading along the zigzag and armchair directions of black phosphorus,respectively.The Raman shift-strain relationship of black phosphorus was derived,and an interface transfer model was developed for the heterostructure.Based on the model,the strain transfer efficiency of each measuring spot was calculated and the strain transfer mechanism of each layer was analyzed.The results uncover the influence of the anisotropic interlayer properties inside the black phosphorus on the strain transfer behavior in the heterostructure on the flexible substrate.展开更多
Vitamin B_(6),a vital micronutrient acquired through dietary intake,plays a crucial role in numerous enzymatic reactions within the human body.Despite its significance,deficiencies in vitamin B_(6) remain prevalent an...Vitamin B_(6),a vital micronutrient acquired through dietary intake,plays a crucial role in numerous enzymatic reactions within the human body.Despite its significance,deficiencies in vitamin B_(6) remain prevalent and are linked to a spectrum of chronic and acute diseases.This review explored the intricate relationships between vitamin B_(6) metabolism and various diseases,focusing on cancer,diabetes,cardiovascular conditions,neurodegenerative disorders,and COVID-19-related complications.We highlighted the mechanistic roles of pyridoxal 5′-phosphate,the active form of vitamin B_(6),in processes such as inflammation modulation,homocysteine regulation,and oxidative stress mitigation.By synthesizing recent advances in both clinical and preclinical studies,this paper underscores the therapeutic potential of vitamin B_(6) while advocating for personalized nutritional interventions tailored to individual health profiles.Our findings aim to inform future research,foster targeted disease prevention strategies,and optimize the safe use of vitamin B_(6) as part of a balanced nutritional approach.展开更多
Epigallocatechin-3-gallate （EGCG）, a naturally occurring compound in green tea, has been widely used as an antioxidant agent. In the present study, model rats with acute spinal cord injury were intraperitoneally inj...Epigallocatechin-3-gallate （EGCG）, a naturally occurring compound in green tea, has been widely used as an antioxidant agent. In the present study, model rats with acute spinal cord injury were intraperitoneally injected with 25, 50, and 100 mg/kg EGCG, and spinal cord ultrastructure, oxidative stress reaction, inflammatory factors, and apoptosis-associated gene expression were observed. Results showed that EGCG attenuated neuronal and axonal injury 24 hours post injury. It also decreased serum intedeukin-113, tumor necrosis factor-a, and intercellular adhesion molecule-1 release, and decreased apoptosis-associated gene expression. Furthermore, it increased the level of the superoxide anion （O2-）, superoxide dismutase, and B-cell lymphoma/leukemia-2, and reduced malondialdehyde levels. Furthermore, it reduced the expression of the pro-apoptotic protein Bax. Noticeably, EGCG at the 100 mg/kg dosage exhibited similar effects as methylprednisolone sodium succinate, which has been frequently used for clinical acute spinal cord injury. The results demonstrated that EGCG can significantly inhibit inflammation, suppress oxidation, and reduce apoptosis in acute spinal cord injury.展开更多
Optical time-stretch(OTS)imaging flow cytometry offers a promising solution for high-throughput and highprecision cell analysis due to its capabilities of high-speed,high-quality,and continuous imaging.Compressed sens...Optical time-stretch(OTS)imaging flow cytometry offers a promising solution for high-throughput and highprecision cell analysis due to its capabilities of high-speed,high-quality,and continuous imaging.Compressed sensing(CS)makes it practically applicable by significantly reducing the data volume while maintaining its highspeed and high-quality imaging properties.To enrich the information of the images acquired with CS-equipped OTS imaging flow cytometry,in this work we propose and experimentally demonstrate Fourier-domaincompressed OTS quantitative phase imaging flow cytometry.It is capable of acquiring intensity and quantitative phase images of cells simultaneously from the compressed data.To evaluate the performance of our method,static microparticles and a corn root cross section are experimentally measured under various compression ratios.Furthermore,to show how our method can be applied in practice,we utilize it in the drug response analysis of breast cancer cells.Experimental results show that our method can acquire high-quality intensity and quantitative phase images of flowing cells at a flowing speed of 1 m/s and a compression ratio of 30%.Combined with machine-learning-based image analysis,it can distinguish drug-treated and drug-untreated cells with an accuracy of over 95%.We believe our method can facilitate cell analysis in both scientific research and clinical settings where both high-throughput and high-content cell analysis is required.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.12125203,12021002,and 12041201).
文摘The strain transfer behavior of graphene and black phosphorus heterostructure on fexible substrates plays a crucial role in the functionality and regulation of the device.Specifically,it is imperative to investigate the anisotropy associated with strain transfer at the black phosphorus interface.In this study,a sample transfer method was proposed to prevent the contact of black phosphorus with water,achieving monolayer graphene and few-layer black phosphorus heterostructures on a PET flm substrate.Micro-Raman spectroscopy was used to measure the strain of graphene and black phosphorus when the PET flm substrate was under uniaxial tensile loading along the zigzag and armchair directions of black phosphorus,respectively.The Raman shift-strain relationship of black phosphorus was derived,and an interface transfer model was developed for the heterostructure.Based on the model,the strain transfer efficiency of each measuring spot was calculated and the strain transfer mechanism of each layer was analyzed.The results uncover the influence of the anisotropic interlayer properties inside the black phosphorus on the strain transfer behavior in the heterostructure on the flexible substrate.
文摘Vitamin B_(6),a vital micronutrient acquired through dietary intake,plays a crucial role in numerous enzymatic reactions within the human body.Despite its significance,deficiencies in vitamin B_(6) remain prevalent and are linked to a spectrum of chronic and acute diseases.This review explored the intricate relationships between vitamin B_(6) metabolism and various diseases,focusing on cancer,diabetes,cardiovascular conditions,neurodegenerative disorders,and COVID-19-related complications.We highlighted the mechanistic roles of pyridoxal 5′-phosphate,the active form of vitamin B_(6),in processes such as inflammation modulation,homocysteine regulation,and oxidative stress mitigation.By synthesizing recent advances in both clinical and preclinical studies,this paper underscores the therapeutic potential of vitamin B_(6) while advocating for personalized nutritional interventions tailored to individual health profiles.Our findings aim to inform future research,foster targeted disease prevention strategies,and optimize the safe use of vitamin B_(6) as part of a balanced nutritional approach.
文摘Epigallocatechin-3-gallate （EGCG）, a naturally occurring compound in green tea, has been widely used as an antioxidant agent. In the present study, model rats with acute spinal cord injury were intraperitoneally injected with 25, 50, and 100 mg/kg EGCG, and spinal cord ultrastructure, oxidative stress reaction, inflammatory factors, and apoptosis-associated gene expression were observed. Results showed that EGCG attenuated neuronal and axonal injury 24 hours post injury. It also decreased serum intedeukin-113, tumor necrosis factor-a, and intercellular adhesion molecule-1 release, and decreased apoptosis-associated gene expression. Furthermore, it increased the level of the superoxide anion （O2-）, superoxide dismutase, and B-cell lymphoma/leukemia-2, and reduced malondialdehyde levels. Furthermore, it reduced the expression of the pro-apoptotic protein Bax. Noticeably, EGCG at the 100 mg/kg dosage exhibited similar effects as methylprednisolone sodium succinate, which has been frequently used for clinical acute spinal cord injury. The results demonstrated that EGCG can significantly inhibit inflammation, suppress oxidation, and reduce apoptosis in acute spinal cord injury.
基金National Key Research and Development Program of China(2023YFF0723300)Fundamental Research Funds for the Central Universities(2042023kf0105,2042024kf0003,2042024kf1010)+6 种基金Science Fund for Distinguished Young Scholars of Hubei Province(2021CFA042)Natural Science Foundation of Hubei Province(2023AFB133)National Natural Science Foundation of China(12374295,62075200)Interdisciplinary Innovative Talents Foundation from Renmin Hospital of Wuhan University(JCRCYR-2022-006)Jiangsu Science and Technology Program(BK20221257)Shenzhen Science and Technology Program(JCYJ20220530140601003,JCYJ20230807090207014)Translational Medicine and Multidisciplinary Research Project of Zhongnan Hospital of Wuhan University(ZNJC202217)。
文摘Optical time-stretch(OTS)imaging flow cytometry offers a promising solution for high-throughput and highprecision cell analysis due to its capabilities of high-speed,high-quality,and continuous imaging.Compressed sensing(CS)makes it practically applicable by significantly reducing the data volume while maintaining its highspeed and high-quality imaging properties.To enrich the information of the images acquired with CS-equipped OTS imaging flow cytometry,in this work we propose and experimentally demonstrate Fourier-domaincompressed OTS quantitative phase imaging flow cytometry.It is capable of acquiring intensity and quantitative phase images of cells simultaneously from the compressed data.To evaluate the performance of our method,static microparticles and a corn root cross section are experimentally measured under various compression ratios.Furthermore,to show how our method can be applied in practice,we utilize it in the drug response analysis of breast cancer cells.Experimental results show that our method can acquire high-quality intensity and quantitative phase images of flowing cells at a flowing speed of 1 m/s and a compression ratio of 30%.Combined with machine-learning-based image analysis,it can distinguish drug-treated and drug-untreated cells with an accuracy of over 95%.We believe our method can facilitate cell analysis in both scientific research and clinical settings where both high-throughput and high-content cell analysis is required.
