Objective:To screen antimicrobial potence of some pipefish species collected from Tuticorin coastal environment.Methods:Antimicrobial activity of pipefishes in methanol extract was investigated against 10 bacterial an...Objective:To screen antimicrobial potence of some pipefish species collected from Tuticorin coastal environment.Methods:Antimicrobial activity of pipefishes in methanol extract was investigated against 10 bacterial and 10 fungal human pathogenic strains.Results:Among the tested strains,in Centriscus scutatus,pipefish showed maximum zone of inhibition against Vibrio cholerae(8 mm)and minimum in the sample of Hippichthys cyanospilos against Klebseilla pneumoniae(2 mm).In positive control,maximum zone of inhibition was recorded in Vibrio cholerae(9 mm)and minimum in Klebseilla pneumoniae,and Salmonella paratyphi(5 mm).Chemical investigation indicated the presence of peptides as evidenced by ninhydrin positive spots on thin layer chromatography and presence of peptide.In SDS PAGE,in Centriscus scutatus,four bands were detected in the gel that represented the presence of proteins in the range nearly 25.8-75 kDa.In Hippichthys cyanospilos,five bands were detected in the gel that represented the presence of proteins in the range nearly 20.5-78 kDa.The result of FT-IR spectrum revealed that the pipe fishes extracts compriseed to have peptide derivatives as their predominant chemical groups.Conclusions:It can be conclude that this present investigation suggests the tested pipe fishes will be a potential source of natural bioactive compounds.展开更多
基金Supported by the University Grants Commission,Government of India,New Delhi[Grant F.37-36/2009(SR)].
文摘Objective:To screen antimicrobial potence of some pipefish species collected from Tuticorin coastal environment.Methods:Antimicrobial activity of pipefishes in methanol extract was investigated against 10 bacterial and 10 fungal human pathogenic strains.Results:Among the tested strains,in Centriscus scutatus,pipefish showed maximum zone of inhibition against Vibrio cholerae(8 mm)and minimum in the sample of Hippichthys cyanospilos against Klebseilla pneumoniae(2 mm).In positive control,maximum zone of inhibition was recorded in Vibrio cholerae(9 mm)and minimum in Klebseilla pneumoniae,and Salmonella paratyphi(5 mm).Chemical investigation indicated the presence of peptides as evidenced by ninhydrin positive spots on thin layer chromatography and presence of peptide.In SDS PAGE,in Centriscus scutatus,four bands were detected in the gel that represented the presence of proteins in the range nearly 25.8-75 kDa.In Hippichthys cyanospilos,five bands were detected in the gel that represented the presence of proteins in the range nearly 20.5-78 kDa.The result of FT-IR spectrum revealed that the pipe fishes extracts compriseed to have peptide derivatives as their predominant chemical groups.Conclusions:It can be conclude that this present investigation suggests the tested pipe fishes will be a potential source of natural bioactive compounds.
