Background:Nonalcoholic fatty liver disease(NAFLD)is one of the most common chronic liver diseases globally.Hepatic stellate cells(HSCs)are the major effector cells of liver fibrosis.HSCs contain abundant lipid drople...Background:Nonalcoholic fatty liver disease(NAFLD)is one of the most common chronic liver diseases globally.Hepatic stellate cells(HSCs)are the major effector cells of liver fibrosis.HSCs contain abundant lipid droplets(LDs)in their cytoplasm during quiescence.Perilipin 5(PLIN 5)is a LD surface-associated protein that plays a crucial role in lipid homeostasis.However,little is known about the role of PLIN 5 in HSC activation.Methods:PLIN 5 was overexpressed in HSCs of Sprague–Dawley rats by lentivirus transfection.At the same time,PLIN 5 gene knockout mice were constructed and fed with a high-fat diet(HFD)for 20 weeks to study the role of PLIN 5 in NAFLD.The corresponding reagent kits were used to measure TG,GSH,Caspase 3 activity,ATP level,and mitochondrial DNA copy number.Metabolomic analysis of mice liver tissue metabolism was performed based on UPLC-MS/MS.AMPK,mitochondrial function,cell proliferation,and apoptosis-related genes and proteins were detected by western blotting and qPCR.Results:Overexpression of PLIN 5 in activated HSCs led to a decrease in ATP levels in mitochondria,inhibition of cell proliferation,and a significant increase in cell apoptosis through AMPK activation.In addition,compared with the HFD-fed C57BL/6J mice,PLIN 5 knockout mice fed with HFD showed reduced liver fat deposition,decreased LD abundance and size,and reduced liver fibrosis.Conclusion:These findings highlight the unique regulatory role of PLIN 5 in HSCs and the role of PLIN 5 in the fibrosis process of NAFLD.展开更多
Background and Objectives:We aimed to explore the relationship between dietary patterns and gestational diabetes mellitus(GDM)during pre-pregnancy six months using principal component analysis(PCA)and the geometric fr...Background and Objectives:We aimed to explore the relationship between dietary patterns and gestational diabetes mellitus(GDM)during pre-pregnancy six months using principal component analysis(PCA)and the geometric framework for nutrition(GFN).Methods and Study Design:We conducted a case-control study that included 210 GDM pregnant women and 210 controls.The dietary intake of all participants was assessed by a validated semi-quantitative food frequency questionnaire(FFQ).Major dietary patterns were extracted by PCA.A conditional logistic regression model was used to determine whether specific dietary patterns are associated with the risk of GDM.Meanwhile,the relationship between dietary patterns and GDM was visualized using GFN.Results:Four major dietary patterns were identified:“protein-rich pattern,”“plant-based pattern,”“oil-picklesdesserts pattern,”and“cereals-nuts pattern.”After adjustment for confounders,the“plant-based pattern”was associated with decreased risk of GDM(Q4 vs.Q1:OR=0.01,95%CI:0.00~0.08),whereas no significant association was found in other dietary patterns.Moreover,there was no dietary intake of ice cream cones and deep-fried dough sticks for the population,which would produce fewer patients with GDM.Deep-fried dough sticks had statistically significant differences in the case and control groups(p<0.001),while ice cream cones had the opposite result.Conclusions:The“plant-based pattern”may reduce the risk of GDM.Besides,although the“cerealsnuts pattern”had no association with GDM risk,avoiding the intake of deep-fried dough sticks could decrease GDM risk.展开更多
Dear Editor,Malnutrition is a prevalent disease in oncology practice(Arends et al.,2017).With its cancer-specific prevalence ranging from 21%–72%,malnutrition is responsible for 10%–20% cancer deaths(Yin et al.,2021...Dear Editor,Malnutrition is a prevalent disease in oncology practice(Arends et al.,2017).With its cancer-specific prevalence ranging from 21%–72%,malnutrition is responsible for 10%–20% cancer deaths(Yin et al.,2021b).However,malnutrition is often underestimated,misclassified,or left untreated in cancer care(Hébuterne et al.,2014).展开更多
From late January to March,more than 42,000 doctors,nurses,public health workers,and health administrators across China went to Hubei Province to assist in the response to coronavirus disease 2019(COVID-19).There are ...From late January to March,more than 42,000 doctors,nurses,public health workers,and health administrators across China went to Hubei Province to assist in the response to coronavirus disease 2019(COVID-19).There are many studies on nutrition status of affected people amid natural disasters or pandemics(1–3),but the diet and nutrition status of healthcare workers receive little attention.The aim of this article is to provide evidence for addressing this issue by investigating food supply and nutrition status during the COVID-19 response period.展开更多
基金Discipline Key Special ProjectGrant/Award Number:XKZDQY202001+7 种基金Henan Provincial Key R&D and Promotion Special ProjectGrant/Award Number:212102310033Henan Provincial Medical Science and Technology Tackling ProgramGrant/Award Number:LHGJ20220557Key R&D Program of ChinaGrant/Award Number:2020YFC2006100,2020YFC2009000 and 2020YFC2009006National Natural Science Foundation of ChinaGrant/Award Number:31471330 and 81870408。
文摘Background:Nonalcoholic fatty liver disease(NAFLD)is one of the most common chronic liver diseases globally.Hepatic stellate cells(HSCs)are the major effector cells of liver fibrosis.HSCs contain abundant lipid droplets(LDs)in their cytoplasm during quiescence.Perilipin 5(PLIN 5)is a LD surface-associated protein that plays a crucial role in lipid homeostasis.However,little is known about the role of PLIN 5 in HSC activation.Methods:PLIN 5 was overexpressed in HSCs of Sprague–Dawley rats by lentivirus transfection.At the same time,PLIN 5 gene knockout mice were constructed and fed with a high-fat diet(HFD)for 20 weeks to study the role of PLIN 5 in NAFLD.The corresponding reagent kits were used to measure TG,GSH,Caspase 3 activity,ATP level,and mitochondrial DNA copy number.Metabolomic analysis of mice liver tissue metabolism was performed based on UPLC-MS/MS.AMPK,mitochondrial function,cell proliferation,and apoptosis-related genes and proteins were detected by western blotting and qPCR.Results:Overexpression of PLIN 5 in activated HSCs led to a decrease in ATP levels in mitochondria,inhibition of cell proliferation,and a significant increase in cell apoptosis through AMPK activation.In addition,compared with the HFD-fed C57BL/6J mice,PLIN 5 knockout mice fed with HFD showed reduced liver fat deposition,decreased LD abundance and size,and reduced liver fibrosis.Conclusion:These findings highlight the unique regulatory role of PLIN 5 in HSCs and the role of PLIN 5 in the fibrosis process of NAFLD.
基金funded and supported by the Chinese Nutrition Society-Feihe Physical Nutrition and Health Research Fund project(Project No.CNS-Feihe2019A34).
文摘Background and Objectives:We aimed to explore the relationship between dietary patterns and gestational diabetes mellitus(GDM)during pre-pregnancy six months using principal component analysis(PCA)and the geometric framework for nutrition(GFN).Methods and Study Design:We conducted a case-control study that included 210 GDM pregnant women and 210 controls.The dietary intake of all participants was assessed by a validated semi-quantitative food frequency questionnaire(FFQ).Major dietary patterns were extracted by PCA.A conditional logistic regression model was used to determine whether specific dietary patterns are associated with the risk of GDM.Meanwhile,the relationship between dietary patterns and GDM was visualized using GFN.Results:Four major dietary patterns were identified:“protein-rich pattern,”“plant-based pattern,”“oil-picklesdesserts pattern,”and“cereals-nuts pattern.”After adjustment for confounders,the“plant-based pattern”was associated with decreased risk of GDM(Q4 vs.Q1:OR=0.01,95%CI:0.00~0.08),whereas no significant association was found in other dietary patterns.Moreover,there was no dietary intake of ice cream cones and deep-fried dough sticks for the population,which would produce fewer patients with GDM.Deep-fried dough sticks had statistically significant differences in the case and control groups(p<0.001),while ice cream cones had the opposite result.Conclusions:The“plant-based pattern”may reduce the risk of GDM.Besides,although the“cerealsnuts pattern”had no association with GDM risk,avoiding the intake of deep-fried dough sticks could decrease GDM risk.
基金supported in part by the National Key Research and Development Program(2017YFC1309200)the National Natural Science Foundation of China(81673167)。
文摘Dear Editor,Malnutrition is a prevalent disease in oncology practice(Arends et al.,2017).With its cancer-specific prevalence ranging from 21%–72%,malnutrition is responsible for 10%–20% cancer deaths(Yin et al.,2021b).However,malnutrition is often underestimated,misclassified,or left untreated in cancer care(Hébuterne et al.,2014).
文摘From late January to March,more than 42,000 doctors,nurses,public health workers,and health administrators across China went to Hubei Province to assist in the response to coronavirus disease 2019(COVID-19).There are many studies on nutrition status of affected people amid natural disasters or pandemics(1–3),but the diet and nutrition status of healthcare workers receive little attention.The aim of this article is to provide evidence for addressing this issue by investigating food supply and nutrition status during the COVID-19 response period.
