Mechanical allodynia(MA),including punctate and dynamic forms,is a common and debilitating symptom suffered by millions of chronic pain patients.Some peripheral injuries result in the development of bilateral MA,while...Mechanical allodynia(MA),including punctate and dynamic forms,is a common and debilitating symptom suffered by millions of chronic pain patients.Some peripheral injuries result in the development of bilateral MA,while most injuries usually led to unilateral MA.To date,the control of such laterality remains poorly understood.Here,to study the role of microglia in the control of MA laterality,we used genetic strategies to deplete microglia and tested both dynamic and punctate forms of MA in mice.Surprisingly,the depletion of central microglia did not prevent the induction of bilateral dynamic and punctate MA.Moreover,in dorsal root ganglion-dorsal root-sagittal spinal cord slice preparations we recorded the low-threshold Aβ-fiber stimulation-evoked inputs and outputs of superficial dorsal horn neurons.Consistent with behavioral results,microglial depletion did not prevent the opening of bilateral gates for Aβpathways in the superficial dorsal horn.This study challenges the role of microglia in the control of MA laterality in mice.Future studies are needed to further understand whether the role of microglia in the control of MA laterality is etiology-or species-specific.展开更多
The original version of this article unfortunately contained one error.The authors found that in the middle panel of Fig.2A,the example traces recorded from a contralateral dorsal horn neuron of the capsaicin-treated ...The original version of this article unfortunately contained one error.The authors found that in the middle panel of Fig.2A,the example traces recorded from a contralateral dorsal horn neuron of the capsaicin-treated mouse were wrong,and the corrected Fig.2 was as follows.展开更多
This article studies the wall temperature distribution of inorganic heat transfer element in different working conditions by experiments, and analyzes the impact of inclination angle, heating power, different kinds of...This article studies the wall temperature distribution of inorganic heat transfer element in different working conditions by experiments, and analyzes the impact of inclination angle, heating power, different kinds of cooling medium and different inlet temperature of cooling medium on the starting property of inorganic heat transfer element.展开更多
[Objectives]To establish the quality standard of Oxyria sinensis Hemsl.[Methods]The method in Chinese Pharmacopoeia(2020 edition)was used to determine the moisture,total ash,acid-insoluble ash and alcohol-soluble extr...[Objectives]To establish the quality standard of Oxyria sinensis Hemsl.[Methods]The method in Chinese Pharmacopoeia(2020 edition)was used to determine the moisture,total ash,acid-insoluble ash and alcohol-soluble extracts of O.sinensis.The qualitative identification of medicinal materials was carried out by microscopic identification method and thin layer chromatography(TLC).The contents of hyperoside and isoquercitrin were determined by high performance liquid chromatography(HPLC).[Results]The total amount of moisture,total ash,acid-insoluble ash,alcohol-soluble extract,hyperoside and isoquercitrin of 12 batches of medicinal materials were 8.56%-11.53%,11.26%-15.48%,1.62%-2.56%,17.75%-26.09%,and 0.036%-0.42%,respectively.The microscopic features were obvious,and pores,fibers,calcium oxalate clusters,calcium oxalate square crystals,cork cells could be observed.Fluorescent spots of the same color were displayed at the positions corresponding to the hyperoside reference substance.[Conclusions]Establishing relatively complete quality standards for O.sinensis medicinal materials can provide a reference for the quality control of medicinal materials.展开更多
[Objectives]To establish the quality standard for Hibiscus trionum L.[Methods]The moisture,total ash,acid insoluble ash and extract of H.trionum L.were determined by the method of Chinese Pharmacopoeia 2020 edition.Th...[Objectives]To establish the quality standard for Hibiscus trionum L.[Methods]The moisture,total ash,acid insoluble ash and extract of H.trionum L.were determined by the method of Chinese Pharmacopoeia 2020 edition.The medicinal materials were qualitatively identified by microscopic identification and TLC,and the content of quercetin was determined by HPLC.[Results]The moisture,total ash,acid insoluble ash and extract contents of the 12 batches of samples were 7.69%-12.94%,10.44%-14.62%,1.89%-3.64%,16.56%-21.81%,microscopic characteristic was obvious,and a method for TLC and content determination of H.trionum L.was established.[Conclusions]A perfect quality standard was established,which can be used for the quality control of H.trionum L.展开更多
基金supported by grants from the Ministry of Science and Technology of China(2021ZD0203302)the National Natural Science Foundation of China(32170996,32060199)+3 种基金the Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions(2021SHIBS0002)the Guangdong Science and Technology Committee(2019A1515010041,A2021319)the Shenzhen Innovation Committee of Science and Technology(ZDSYS20200811144002008)the Shenzhen Science and Technology Innovation Committee(JCYJ20180302174233348).
文摘Mechanical allodynia(MA),including punctate and dynamic forms,is a common and debilitating symptom suffered by millions of chronic pain patients.Some peripheral injuries result in the development of bilateral MA,while most injuries usually led to unilateral MA.To date,the control of such laterality remains poorly understood.Here,to study the role of microglia in the control of MA laterality,we used genetic strategies to deplete microglia and tested both dynamic and punctate forms of MA in mice.Surprisingly,the depletion of central microglia did not prevent the induction of bilateral dynamic and punctate MA.Moreover,in dorsal root ganglion-dorsal root-sagittal spinal cord slice preparations we recorded the low-threshold Aβ-fiber stimulation-evoked inputs and outputs of superficial dorsal horn neurons.Consistent with behavioral results,microglial depletion did not prevent the opening of bilateral gates for Aβpathways in the superficial dorsal horn.This study challenges the role of microglia in the control of MA laterality in mice.Future studies are needed to further understand whether the role of microglia in the control of MA laterality is etiology-or species-specific.
文摘The original version of this article unfortunately contained one error.The authors found that in the middle panel of Fig.2A,the example traces recorded from a contralateral dorsal horn neuron of the capsaicin-treated mouse were wrong,and the corrected Fig.2 was as follows.
文摘This article studies the wall temperature distribution of inorganic heat transfer element in different working conditions by experiments, and analyzes the impact of inclination angle, heating power, different kinds of cooling medium and different inlet temperature of cooling medium on the starting property of inorganic heat transfer element.
基金Supported by Chinese Medicine(Ethnic Medicine)Standard Improvement Project of Sichuan Medical Products Administration(510201202102305)School Level Innovation Team of Fundamental Research Funds for the Central Universities(ZYN2022067)。
文摘[Objectives]To establish the quality standard of Oxyria sinensis Hemsl.[Methods]The method in Chinese Pharmacopoeia(2020 edition)was used to determine the moisture,total ash,acid-insoluble ash and alcohol-soluble extracts of O.sinensis.The qualitative identification of medicinal materials was carried out by microscopic identification method and thin layer chromatography(TLC).The contents of hyperoside and isoquercitrin were determined by high performance liquid chromatography(HPLC).[Results]The total amount of moisture,total ash,acid-insoluble ash,alcohol-soluble extract,hyperoside and isoquercitrin of 12 batches of medicinal materials were 8.56%-11.53%,11.26%-15.48%,1.62%-2.56%,17.75%-26.09%,and 0.036%-0.42%,respectively.The microscopic features were obvious,and pores,fibers,calcium oxalate clusters,calcium oxalate square crystals,cork cells could be observed.Fluorescent spots of the same color were displayed at the positions corresponding to the hyperoside reference substance.[Conclusions]Establishing relatively complete quality standards for O.sinensis medicinal materials can provide a reference for the quality control of medicinal materials.
基金Supported by National Key R&D Plan(2018YFC1706101)Sichuan Science and Technology Plan Project(2021YFS0043)+1 种基金2021 State Ethnic Affairs Commission Leading Talent Support PlanBasic Scientific Research Project for Central Universities—Special Project of School-level Innovation Team(ZYN2022067)。
文摘[Objectives]To establish the quality standard for Hibiscus trionum L.[Methods]The moisture,total ash,acid insoluble ash and extract of H.trionum L.were determined by the method of Chinese Pharmacopoeia 2020 edition.The medicinal materials were qualitatively identified by microscopic identification and TLC,and the content of quercetin was determined by HPLC.[Results]The moisture,total ash,acid insoluble ash and extract contents of the 12 batches of samples were 7.69%-12.94%,10.44%-14.62%,1.89%-3.64%,16.56%-21.81%,microscopic characteristic was obvious,and a method for TLC and content determination of H.trionum L.was established.[Conclusions]A perfect quality standard was established,which can be used for the quality control of H.trionum L.
