To evaluate the potential anti-aging ability of Eucommia ulmoides,four characteristic components(chlorogenic acid,geniposidic acid,aucubin,quercetin)were selected to assess their effects on H_(2)O_(2)-induced oxidativ...To evaluate the potential anti-aging ability of Eucommia ulmoides,four characteristic components(chlorogenic acid,geniposidic acid,aucubin,quercetin)were selected to assess their effects on H_(2)O_(2)-induced oxidative damage model of human umbilical vein endothelial cell(HUVEC).Oxidative damage indexes,inflammatory factors,cell cycle,cell apoptosis,cell senescence,and their related proteins were analyzed by methyl thiazolyl tetrazolium(MTT)assay,enzyme-linked immunosorbent assay(ELISA),propidium iodide(PI)staining,annexin V-FITC/PI double staining,SAβ-galactosidase staining,and western blotting(WB).The results showed that H_(2)O_(2)-induced cell growth inhibition rate decreased as supplementation with characteristic components when compared to H_(2)O_(2) group.Meanwhile,the contents of antioxidant indexes(reactive oxygen species,lactate dehydrogenase,molondialdehyde,superoxide dismutase,glutathione),inflammatory factors(nuclear factor kappa-B,intercellular cell adhesion molecule-1,vascular cell adhesion protein 1),and functional factors(NO,Endothelin-1)in characteristic components treated groups improved if comparison with H_(2)O_(2) group,suggesting the characteristic components of E.ulmoides could alleviate H_(2)O_(2)-induced oxidative damage.Moreover,cell cycle,cell apoptosis,cell senescence,and their related proteins under characteristic components treatment exhibited a better effect than under H_(2)O_(2) treatment,implying the characteristic components could participate in anti-aging via multiple pathways.These results manifested that the characteristic components of E.ulmoides posses the capacity of anti-aging,which provided a basis for investigating the anti-aging ability of E.ulmoides itself.展开更多
Delay and stability are two key factors that affect the performance of multicast data transmission in a network.However,current algorithms of tree generation hardly meet the requirements of low delay and high sta-bili...Delay and stability are two key factors that affect the performance of multicast data transmission in a network.However,current algorithms of tree generation hardly meet the requirements of low delay and high sta-bility simultaneously.Given a general network,the generation algorithm of a multicast tree with minimum delay and maximum stability is an NP-hard problem,without a precise and efficient algorithm.To address these challenges,this paper studies the generation of low-delay and high-stability multicast trees under the model of spanning tree based on stability probability,degree-constrained,edge-weighted for multicast(T-SDE).A class of algorithms was proposed which creates the multicast tree greedy on the ratio of fan-out to delay(RFD)and probability of stability of terminal to obtain a high performance in multicast.The proposed algorithms greedily select terminals with a large RFD and a high probability of stability as forwarding nodes in the generation of the multicast tree,where the larger RFD and higher stability of upstream nodes are beneficial to achieve a low transmission delay and high stability in multicast.The proposed RFD can be compatible with the original model,which can take advantage of network connectivity during the generation of a multicast tree.This paper carries out simulation experiments on Matlab R2016b to measure the performance of the proposed algorithm.Experimental results show that the proposed algorithm can provide a smaller height,higher stability,and a lower transmission delay of the resulting multicast tree than other solutions.The spanning tree of the proposed algorithms can support low transmission delay and high stability in multicast transmission.展开更多
J-aggregation and H-aggregation are identified as two classical models of function-ally oriented non-covalent interactions,and significant attention has been drawn by researchers.However,due to the scarcity of single-...J-aggregation and H-aggregation are identified as two classical models of function-ally oriented non-covalent interactions,and significant attention has been drawn by researchers.However,due to the scarcity of single-crystal examples of H-aggregation,a comprehensive understanding of the relationship between its stacking mode and optical behaviour has been hindered.In recent studies,two polyaromatic Schiff base compounds,Cl-Salmphen and H-Salmphen,were successfully synthe-sized,and both were found to exhibit H-aggregation.In thefindings,H-Salmphen was shown to display typical C─H···πinteractions,characteristic of Aggregation-Induced Emission(AIE)active molecules,whereas its halogenated counterpart was identified as behaving similar to Aggregation-Caused Quenching(ACQ)active molecules.These types of results suggest that identical intermolecular interactions can produce differing optical behaviours.Light was shed,at least in part,on the for-mation mechanisms of H-type aggregates and their luminescence properties from these observations.Additionally,the high optical signal-to-noise ratio inherent to H-aggregates was utilized for the exploration of water content detection.As an outcome,a high-performancefluorescentfilter paper was developed,enabling easy real-time detection using a smartphone.展开更多
基金This study was supported by the National Natural Science Foundation of China(Grant No.42107020)the Science and Technology Project of Hunan Province(2020SK2028).
文摘To evaluate the potential anti-aging ability of Eucommia ulmoides,four characteristic components(chlorogenic acid,geniposidic acid,aucubin,quercetin)were selected to assess their effects on H_(2)O_(2)-induced oxidative damage model of human umbilical vein endothelial cell(HUVEC).Oxidative damage indexes,inflammatory factors,cell cycle,cell apoptosis,cell senescence,and their related proteins were analyzed by methyl thiazolyl tetrazolium(MTT)assay,enzyme-linked immunosorbent assay(ELISA),propidium iodide(PI)staining,annexin V-FITC/PI double staining,SAβ-galactosidase staining,and western blotting(WB).The results showed that H_(2)O_(2)-induced cell growth inhibition rate decreased as supplementation with characteristic components when compared to H_(2)O_(2) group.Meanwhile,the contents of antioxidant indexes(reactive oxygen species,lactate dehydrogenase,molondialdehyde,superoxide dismutase,glutathione),inflammatory factors(nuclear factor kappa-B,intercellular cell adhesion molecule-1,vascular cell adhesion protein 1),and functional factors(NO,Endothelin-1)in characteristic components treated groups improved if comparison with H_(2)O_(2) group,suggesting the characteristic components of E.ulmoides could alleviate H_(2)O_(2)-induced oxidative damage.Moreover,cell cycle,cell apoptosis,cell senescence,and their related proteins under characteristic components treatment exhibited a better effect than under H_(2)O_(2) treatment,implying the characteristic components could participate in anti-aging via multiple pathways.These results manifested that the characteristic components of E.ulmoides posses the capacity of anti-aging,which provided a basis for investigating the anti-aging ability of E.ulmoides itself.
基金supported by the Hainan Provincial Natural Science Foundation of China(620RC560,2019RC096,620RC562)the Scientific Research Setup Fund of Hainan University(KYQD(ZR)1877)+2 种基金the National Natural Science Foundation of China(62162021,61802092,82160345,61862020)the key research and development program of Hainan province(ZDYF2020199,ZDYF2021GXJS017)the key science and technology plan project of Haikou(2011-016).
文摘Delay and stability are two key factors that affect the performance of multicast data transmission in a network.However,current algorithms of tree generation hardly meet the requirements of low delay and high sta-bility simultaneously.Given a general network,the generation algorithm of a multicast tree with minimum delay and maximum stability is an NP-hard problem,without a precise and efficient algorithm.To address these challenges,this paper studies the generation of low-delay and high-stability multicast trees under the model of spanning tree based on stability probability,degree-constrained,edge-weighted for multicast(T-SDE).A class of algorithms was proposed which creates the multicast tree greedy on the ratio of fan-out to delay(RFD)and probability of stability of terminal to obtain a high performance in multicast.The proposed algorithms greedily select terminals with a large RFD and a high probability of stability as forwarding nodes in the generation of the multicast tree,where the larger RFD and higher stability of upstream nodes are beneficial to achieve a low transmission delay and high stability in multicast.The proposed RFD can be compatible with the original model,which can take advantage of network connectivity during the generation of a multicast tree.This paper carries out simulation experiments on Matlab R2016b to measure the performance of the proposed algorithm.Experimental results show that the proposed algorithm can provide a smaller height,higher stability,and a lower transmission delay of the resulting multicast tree than other solutions.The spanning tree of the proposed algorithms can support low transmission delay and high stability in multicast transmission.
基金the Program for Innovative Research Team(in Science and Technology)in Universities of Yunnan Province(IRTSTYN),the Recruitment Program of Yunnan Province Experts Provincial Young Talents(2019HB098)the Ten-Thousand Talents Program of Yunnan Province(YNWR-QNBJ-2018-273).
文摘J-aggregation and H-aggregation are identified as two classical models of function-ally oriented non-covalent interactions,and significant attention has been drawn by researchers.However,due to the scarcity of single-crystal examples of H-aggregation,a comprehensive understanding of the relationship between its stacking mode and optical behaviour has been hindered.In recent studies,two polyaromatic Schiff base compounds,Cl-Salmphen and H-Salmphen,were successfully synthe-sized,and both were found to exhibit H-aggregation.In thefindings,H-Salmphen was shown to display typical C─H···πinteractions,characteristic of Aggregation-Induced Emission(AIE)active molecules,whereas its halogenated counterpart was identified as behaving similar to Aggregation-Caused Quenching(ACQ)active molecules.These types of results suggest that identical intermolecular interactions can produce differing optical behaviours.Light was shed,at least in part,on the for-mation mechanisms of H-type aggregates and their luminescence properties from these observations.Additionally,the high optical signal-to-noise ratio inherent to H-aggregates was utilized for the exploration of water content detection.As an outcome,a high-performancefluorescentfilter paper was developed,enabling easy real-time detection using a smartphone.
