As a well-known natural protein biomaterial,silk fibroin(SF)has shown broad application prospects in typical biomedical fields.However,the mostly used SF from Bombyx mori silkworm lacks specific cell adhesion sites an...As a well-known natural protein biomaterial,silk fibroin(SF)has shown broad application prospects in typical biomedical fields.However,the mostly used SF from Bombyx mori silkworm lacks specific cell adhesion sites and other bioactive peptide sequences,and there is still significant room for further improvement of their biological functions.Therefore,it is crucial to develop a facile and effective modification strategy for this widely researched biomaterial.In this study,the SF electrospun scaffold has been chosen as a typical SF biomaterial,and air plasma etching has been adopted as a facile nanopattern modification strategy to promote its biological functions.Results demonstrated that the plasma etching could feasibly and effectively create nano-island-like patterns on the complex surface of SF scaffolds,and the detailed nanopattern features could be easily regulated by adjusting the etching time.In addition,the mesenchymal stem cell responses have illustrated that the nanopattern modification could significantly regulate corresponding cell behaviors.Compared with the non-etched scaffold,the 10min-etched scaffolds(10E scaffold)significantly promoted stem cell proliferation and osteogenic differentiation.Moreover,10E scaffold has also been confirmed to effectively accelerate vascularization and ectopic osteogenesis in vivo using a rat subcutaneous implantation model.However,the mentioned promoting effects would be weakened or even counteracted with the increase of etching time.In conclusion,this facile modification strategy demonstrated great application potential for promoting cell proliferation and differentiation.Thus,it provided useful guidance to develop excellent SF-based scaffolds suitable for bone and other tissue engineering.展开更多
Acute liver failure(ALF)has a mortality rate of more than 40%.Currently,orthotopic liver transplantation is the sole clinical treatment for ALF,but its wide usage is severely limited due to donor shortage and immunolo...Acute liver failure(ALF)has a mortality rate of more than 40%.Currently,orthotopic liver transplantation is the sole clinical treatment for ALF,but its wide usage is severely limited due to donor shortage and immunological rejection.An emerging and promising technology for ALF treatment is liver tissue engineering(LTE),wherein porous scaffolds serve as a crucial component.Nanofiber scaffolds,which mimic the inherent structures of fibrous extracellular matrix well,provide an ideal environment for cell growth and tissue regeneration.Recently,several functional nanofiber scaffolds for LTE have been developed,which show impressive results in regulating cell function and repairing liver injury when combined with appropriate seeding cells and/or growth factors.This review firstly introduces the etiologies and treatment indicators of ALF.Subsequently,typical fabrication technologies of nanofiber scaffolds and their related applications for function regulation of liver-related cells and treatment of ALF are comprehensively summarized.Particular emphasis is placed on the strategies involving an appropriate combination of suitable seeding cells and growth factors.Finally,the current challenges and the future research and development prospects of nanofiber scaffold-based LTE are discussed.This review will serve as a valuable reference for designing and modifying novel nanofiber scaffolds,further promoting their potential application in LTE and other biomedical fields.展开更多
基金supported by the International Cooperation Fund of the Science and Technology Commission of Shanghai Municipality(22520711900)National Natural Science Foundation of China(52273125,52173031)+2 种基金the Fundamental Research Funds for the Central Universities(2232024D-01)the Basic Research Project of the Science and Technology Commission of Shanghai Municipality(21JC1400100)the Oriental Talent Plan(Leading Talent Program,no.152).
文摘As a well-known natural protein biomaterial,silk fibroin(SF)has shown broad application prospects in typical biomedical fields.However,the mostly used SF from Bombyx mori silkworm lacks specific cell adhesion sites and other bioactive peptide sequences,and there is still significant room for further improvement of their biological functions.Therefore,it is crucial to develop a facile and effective modification strategy for this widely researched biomaterial.In this study,the SF electrospun scaffold has been chosen as a typical SF biomaterial,and air plasma etching has been adopted as a facile nanopattern modification strategy to promote its biological functions.Results demonstrated that the plasma etching could feasibly and effectively create nano-island-like patterns on the complex surface of SF scaffolds,and the detailed nanopattern features could be easily regulated by adjusting the etching time.In addition,the mesenchymal stem cell responses have illustrated that the nanopattern modification could significantly regulate corresponding cell behaviors.Compared with the non-etched scaffold,the 10min-etched scaffolds(10E scaffold)significantly promoted stem cell proliferation and osteogenic differentiation.Moreover,10E scaffold has also been confirmed to effectively accelerate vascularization and ectopic osteogenesis in vivo using a rat subcutaneous implantation model.However,the mentioned promoting effects would be weakened or even counteracted with the increase of etching time.In conclusion,this facile modification strategy demonstrated great application potential for promoting cell proliferation and differentiation.Thus,it provided useful guidance to develop excellent SF-based scaffolds suitable for bone and other tissue engineering.
基金supported by the National Natural Science Foundation of China(31971263,52173031,52273125)the International Cooperation Fund of the Science and Technology Commission of Shanghai Municipality(22520711900)+1 种基金the Basic Research Project of the Science and Technology Commission of Shanghai Municipality(21JC1400100)the Oriental Talent Plan(Leading Talent Program,No.152).
文摘Acute liver failure(ALF)has a mortality rate of more than 40%.Currently,orthotopic liver transplantation is the sole clinical treatment for ALF,but its wide usage is severely limited due to donor shortage and immunological rejection.An emerging and promising technology for ALF treatment is liver tissue engineering(LTE),wherein porous scaffolds serve as a crucial component.Nanofiber scaffolds,which mimic the inherent structures of fibrous extracellular matrix well,provide an ideal environment for cell growth and tissue regeneration.Recently,several functional nanofiber scaffolds for LTE have been developed,which show impressive results in regulating cell function and repairing liver injury when combined with appropriate seeding cells and/or growth factors.This review firstly introduces the etiologies and treatment indicators of ALF.Subsequently,typical fabrication technologies of nanofiber scaffolds and their related applications for function regulation of liver-related cells and treatment of ALF are comprehensively summarized.Particular emphasis is placed on the strategies involving an appropriate combination of suitable seeding cells and growth factors.Finally,the current challenges and the future research and development prospects of nanofiber scaffold-based LTE are discussed.This review will serve as a valuable reference for designing and modifying novel nanofiber scaffolds,further promoting their potential application in LTE and other biomedical fields.
