Objectives:The tumor microenvironment and epithelial-mesenchymal transition(EMT)are closely linked to the progression of differentiated thyroid cancer(DTC).However,the functional mechanisms of lysine-specific demethyl...Objectives:The tumor microenvironment and epithelial-mesenchymal transition(EMT)are closely linked to the progression of differentiated thyroid cancer(DTC).However,the functional mechanisms of lysine-specific demethylase 6B(KDM6B)in carcinogenesis remain incompletely understood.This study aims to clarify whether KDM6B affects DTC progression and EMT through the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of the rapamycin(PI3K/AKT/mTOR)pathway,providing a potential target for clinical treatment of DTC.Methods:Tissue samples from DTC patients(n=39)were collected,and KDM6B expression was determined through Reverse Transcription-Polymerase Chain Reaction(RT-PCR)and Western blot.Cell counting kit-8 assay,5-Ethynyl-2′-deoxyuridine staining,Transwell,Scratch-Wound,and other experiments were used to detect cell biological behavior;flow cytometry and enzyme-linked immunosorbent assay were used to investigate its effect on macrophage polarization.A subcutaneous tumor model was constructed in mice,and immunohistochemistry was used to detect nuclear proliferation antigen Ki-67,and a Western blot was used to validate protein expression.Results:KDM6B level was elevated in DTC tissues and cells compared to normal ones.Knocking down KDM6B inhibited DTC cell proliferation,reducedmigratory and invasive capabilities,suppressedM2macrophage polarization and EMTprocesses,while overexpression of KDM6B promoted the aforementioned biological behaviors.Knocking down KDM6B blocked the PI3K/AKT/mTOR pathway,while overexpression of KDM6B activated this pathway.PI3K agonists weakened the inhibitory impact of KDM6B knockdown on malignant biological characteristics;the opposite was true for PI3K inhibitors.Additionally,knocking down KDM6B inhibited tumor growth,decreased the Ki67 positivity rate,and inhibited the EMT process and M2 macrophage polarization in mice.Conclusion:KDM6B regulates the tumor microenvironment and EMT process via PI3K/AKT/mTOR pathway,thereby influencing DTC progression.展开更多
基金funded by the Key Scientific Research Project Plan of colleges and universities in Henan Province(No.24B320007).
文摘Objectives:The tumor microenvironment and epithelial-mesenchymal transition(EMT)are closely linked to the progression of differentiated thyroid cancer(DTC).However,the functional mechanisms of lysine-specific demethylase 6B(KDM6B)in carcinogenesis remain incompletely understood.This study aims to clarify whether KDM6B affects DTC progression and EMT through the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of the rapamycin(PI3K/AKT/mTOR)pathway,providing a potential target for clinical treatment of DTC.Methods:Tissue samples from DTC patients(n=39)were collected,and KDM6B expression was determined through Reverse Transcription-Polymerase Chain Reaction(RT-PCR)and Western blot.Cell counting kit-8 assay,5-Ethynyl-2′-deoxyuridine staining,Transwell,Scratch-Wound,and other experiments were used to detect cell biological behavior;flow cytometry and enzyme-linked immunosorbent assay were used to investigate its effect on macrophage polarization.A subcutaneous tumor model was constructed in mice,and immunohistochemistry was used to detect nuclear proliferation antigen Ki-67,and a Western blot was used to validate protein expression.Results:KDM6B level was elevated in DTC tissues and cells compared to normal ones.Knocking down KDM6B inhibited DTC cell proliferation,reducedmigratory and invasive capabilities,suppressedM2macrophage polarization and EMTprocesses,while overexpression of KDM6B promoted the aforementioned biological behaviors.Knocking down KDM6B blocked the PI3K/AKT/mTOR pathway,while overexpression of KDM6B activated this pathway.PI3K agonists weakened the inhibitory impact of KDM6B knockdown on malignant biological characteristics;the opposite was true for PI3K inhibitors.Additionally,knocking down KDM6B inhibited tumor growth,decreased the Ki67 positivity rate,and inhibited the EMT process and M2 macrophage polarization in mice.Conclusion:KDM6B regulates the tumor microenvironment and EMT process via PI3K/AKT/mTOR pathway,thereby influencing DTC progression.
