Patients with gallbladder carcinoma can present with a variety of paraneoplastic syndromes,including Cushing's syndrome,hypercalcemia,acanthosis nigricans,bullous pemphigoid,dermatomyositis and the sign of Leser-T...Patients with gallbladder carcinoma can present with a variety of paraneoplastic syndromes,including Cushing's syndrome,hypercalcemia,acanthosis nigricans,bullous pemphigoid,dermatomyositis and the sign of Leser-Trélat.Surgical resection of the primary tumor results in resolution of these paraneoplastic syndromes.We present a 67-year old female with facial and cervical erythema who was initially diagnosed with dermatomyositis.However,an abdominal computed tomography(CT)and positron emission tomographyCT scan was suspicious for gallbladder carcinoma with lymph node metastasis.After surgical resection,her dermatomyositis was resolved.This case demonstrates that dermatomyositis may be a manifestation of preexisting gallbladder carcinoma.展开更多
AIM:To investigate the protective effect of heme oxygenase-1(HO-1)against H_(2)O_(2)-induced apoptosis in human ARPE-19 cells.METHODS:The lentiviral vector expressing HO-1 was prepared and transfected into apoptotic A...AIM:To investigate the protective effect of heme oxygenase-1(HO-1)against H_(2)O_(2)-induced apoptosis in human ARPE-19 cells.METHODS:The lentiviral vector expressing HO-1 was prepared and transfected into apoptotic ARPE-19 cells induced by H_(2)O_(2).Functional experiments including cell counting kit-8(CCK-8)assay,flow cytometry(FCM)and mitochondrial membrane potential assay were conducted.RESULTS:The ultrastructure of ARPE-19 cells was observed using transmission electron microscope(TEM).It was found that exogenous HO-1 significantly ameliorated H_(2)O_(2)-induced loss of cell viability,apoptosis and intracellular levels of reactive oxygen species(ROS)in ARPE-19 cells.The overexpression of HO-1 facilitated the transfer of nuclear factor erythroid-2-related factor 2(Nrf2)from cytoplasm to nucleus,which in turn upregualted expressions HO-1 and B-cell lymphoma-2(Bcl-2).Furthermore,HO-1 upregulation further inhibited H_(2)O_(2)-induced release of cysteinyl aspartate specific proteinase-3(caspase-3).CONCLUSION:Exogenous HO-1 protect ARPE-19 cells against H_(2)O_(2)-induced oxidative stress by regulating the expressions of Nrf2,HO-1,Bcl-2,and caspase-3.展开更多
文摘Patients with gallbladder carcinoma can present with a variety of paraneoplastic syndromes,including Cushing's syndrome,hypercalcemia,acanthosis nigricans,bullous pemphigoid,dermatomyositis and the sign of Leser-Trélat.Surgical resection of the primary tumor results in resolution of these paraneoplastic syndromes.We present a 67-year old female with facial and cervical erythema who was initially diagnosed with dermatomyositis.However,an abdominal computed tomography(CT)and positron emission tomographyCT scan was suspicious for gallbladder carcinoma with lymph node metastasis.After surgical resection,her dermatomyositis was resolved.This case demonstrates that dermatomyositis may be a manifestation of preexisting gallbladder carcinoma.
基金Supported by Natural Science Foundation of Fujian Province(No.2017J01274)Health Science and Technology Program of Fujian Province(No.2015-ZQNJC-26)Quanzhou Science and Technology Plan Project(No.2016Z053)。
文摘AIM:To investigate the protective effect of heme oxygenase-1(HO-1)against H_(2)O_(2)-induced apoptosis in human ARPE-19 cells.METHODS:The lentiviral vector expressing HO-1 was prepared and transfected into apoptotic ARPE-19 cells induced by H_(2)O_(2).Functional experiments including cell counting kit-8(CCK-8)assay,flow cytometry(FCM)and mitochondrial membrane potential assay were conducted.RESULTS:The ultrastructure of ARPE-19 cells was observed using transmission electron microscope(TEM).It was found that exogenous HO-1 significantly ameliorated H_(2)O_(2)-induced loss of cell viability,apoptosis and intracellular levels of reactive oxygen species(ROS)in ARPE-19 cells.The overexpression of HO-1 facilitated the transfer of nuclear factor erythroid-2-related factor 2(Nrf2)from cytoplasm to nucleus,which in turn upregualted expressions HO-1 and B-cell lymphoma-2(Bcl-2).Furthermore,HO-1 upregulation further inhibited H_(2)O_(2)-induced release of cysteinyl aspartate specific proteinase-3(caspase-3).CONCLUSION:Exogenous HO-1 protect ARPE-19 cells against H_(2)O_(2)-induced oxidative stress by regulating the expressions of Nrf2,HO-1,Bcl-2,and caspase-3.
