Leaf curvature significantly contributes to important economic traits in vegetable crops.The upward-curling leaf phenotype has been consistently observed upon overexpression of a miR156/157-resistant version of the SQ...Leaf curvature significantly contributes to important economic traits in vegetable crops.The upward-curling leaf phenotype has been consistently observed upon overexpression of a miR156/157-resistant version of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 10(SPL10)transcription factor(rSPL10).However,the role of SPL10 in regulating leaf curvature has not been well characterized.In this study,using DNA affinity purification sequencing followed by transient transactivation assays,we found that SPL10 can bind to the promoter and gene body of REVOLUTA(REV),augmenting its expression.The rSPL10 rev-6 double mutant plant displayed a downward-curling leaf phenotype similar to the rev-6 plant,supporting the notion that REV functions downstream of SPL10.Importantly,the SPL10 protein physically interacts with the REV protein,which attenuates the expression of REV promoted by SPL10,leading to the downregulation of REV-regulated genes involved in leaf curvature,such as HB2 and HB4.These findings suggest that the SPL10–REV module acts as a molecular rheostat to prevent excessive amplification of REV transcripts in Arabidopsis.Furthermore,overexpression of the BrpREV1 gene in Chinese cabbage caused the transformation of rosette leaves from flat to upward-curving and accelerated heading.Taken together,our findings reveal the role of SPL10–REV module in orchestrating leaf curvature,which could potentially be utilized for molecular breeding of economical traits in vegetable crops.展开更多
De novo organ regeneration is the process in which adventitious roots or shoots regenerate from detached or wounded organs.De novo organ regeneration can occur either in natural conditions,e.g.adventitious root regene...De novo organ regeneration is the process in which adventitious roots or shoots regenerate from detached or wounded organs.De novo organ regeneration can occur either in natural conditions,e.g.adventitious root regenera-tion from the wounded sites of detached leaves or stems,or in in-vitro tissue culture,e.g.organ regeneration from cal-lus.In this review,we summarize recent advances in research on the molecular mechanism of de novo organ regen-eration,focusing on the role of the WUSCHEL-RELATED HOMEOBOX11(WOX11)gene in the model plant Arabidopsis thaliana.WOX11 is a direct target of the auxin signaling pathway,and it is expressed in,and regulates the establish-ment of,the founder cell during de novo root regeneration and callus formation.WOX11 activates the expression of its target genes to initiate root and callus primordia.Therefore,WOX11 links upstream auxin signaling to downstream cell fate transition during regeneration.We also discuss the role of WOX11 in diverse species and its evolution in plants.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.32170581 and 32370587 to X.Y.,No.32272043 and No.32472190 to S.L.)China Postdoctoral Science Foundation(2022M72211 to P.X.)this research is funded by the Hong Kong Scholars Program.
文摘Leaf curvature significantly contributes to important economic traits in vegetable crops.The upward-curling leaf phenotype has been consistently observed upon overexpression of a miR156/157-resistant version of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 10(SPL10)transcription factor(rSPL10).However,the role of SPL10 in regulating leaf curvature has not been well characterized.In this study,using DNA affinity purification sequencing followed by transient transactivation assays,we found that SPL10 can bind to the promoter and gene body of REVOLUTA(REV),augmenting its expression.The rSPL10 rev-6 double mutant plant displayed a downward-curling leaf phenotype similar to the rev-6 plant,supporting the notion that REV functions downstream of SPL10.Importantly,the SPL10 protein physically interacts with the REV protein,which attenuates the expression of REV promoted by SPL10,leading to the downregulation of REV-regulated genes involved in leaf curvature,such as HB2 and HB4.These findings suggest that the SPL10–REV module acts as a molecular rheostat to prevent excessive amplification of REV transcripts in Arabidopsis.Furthermore,overexpression of the BrpREV1 gene in Chinese cabbage caused the transformation of rosette leaves from flat to upward-curving and accelerated heading.Taken together,our findings reveal the role of SPL10–REV module in orchestrating leaf curvature,which could potentially be utilized for molecular breeding of economical traits in vegetable crops.
基金supported by grants from the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB27030103).
文摘De novo organ regeneration is the process in which adventitious roots or shoots regenerate from detached or wounded organs.De novo organ regeneration can occur either in natural conditions,e.g.adventitious root regenera-tion from the wounded sites of detached leaves or stems,or in in-vitro tissue culture,e.g.organ regeneration from cal-lus.In this review,we summarize recent advances in research on the molecular mechanism of de novo organ regen-eration,focusing on the role of the WUSCHEL-RELATED HOMEOBOX11(WOX11)gene in the model plant Arabidopsis thaliana.WOX11 is a direct target of the auxin signaling pathway,and it is expressed in,and regulates the establish-ment of,the founder cell during de novo root regeneration and callus formation.WOX11 activates the expression of its target genes to initiate root and callus primordia.Therefore,WOX11 links upstream auxin signaling to downstream cell fate transition during regeneration.We also discuss the role of WOX11 in diverse species and its evolution in plants.
