In order to investigate the extraction efficiency of flavonoids by octenyl succinic acid-modified starch(OSA-modified starch)with varying molecular weights,the immature fruit of Citrus paradisi'Changshan Huyou'...In order to investigate the extraction efficiency of flavonoids by octenyl succinic acid-modified starch(OSA-modified starch)with varying molecular weights,the immature fruit of Citrus paradisi'Changshan Huyou',commonly known as"Quzhiqiao",was used as the extraction target in this research.OSA-modified starch was successfully identified and refined,ensuring its molecular weight was appropriate and extraction was optimized.The experimental findings suggested that a concentration of 20 mg/mL of medium molecular weight gelatinized OSA-modified starch was optimal for extracting flavonoids from"Quzhiqiao"using an ultrasound-assisted method(1,000 W,40 min).Additionally,the ratio of the mass of"Quzhiqiao"to the volume of gelatinized OSA-modified starch employed in this process was determined to be 15 mg/mL.The findings from the in vitro antioxidant studies indicated significant variations in the antioxidant activities of the extract solutions of OSA-modified starches with varying molecular weights.These differences can potentially be attributed to substantial variations in the extract solutions composition.展开更多
That herbs with the"hot"property used to treat"cold"syndromes is a guiding principle of clinical prescription and medication in traditional Chinese medicine(TCM).However,this theory of TCM is still...That herbs with the"hot"property used to treat"cold"syndromes is a guiding principle of clinical prescription and medication in traditional Chinese medicine(TCM).However,this theory of TCM is still in the‘black box'stage,and few in-depth studies have examined the biological mechanisms underpinning the hot properties of herbs.展开更多
Recent studies have indicated that suppressing oxidative stress and ferroptosis can considerably improve the prognosis of intracerebral hemorrhage(ICH).Withaferin A(WFA),a natural compound,exhibits a positive effect o...Recent studies have indicated that suppressing oxidative stress and ferroptosis can considerably improve the prognosis of intracerebral hemorrhage(ICH).Withaferin A(WFA),a natural compound,exhibits a positive effect on a number of neurological diseases.However,the effects of WFA on oxidative stress and ferroptosis-mediated signaling pathways to ICH remain unknown.In this study,we investigated the neuroprotective effects and underlying mechanism for WFA in the regulation of ICH-induced oxidative stress and ferroptosis.We established a mouse model of ICH by injection of autologous tail artery blood into the caudate nucleus and an in vitro cell model of hemin-induced ICH.WFA was injected intracerebroventricularly at 0.1,1 or 5μg/kg once daily for 7 days,starting immediately after ICH operation.WFA markedly reduced brain tissue injury and iron deposition and improved neurological function in a dose-dependent manner 7 days after cerebral hemorrhage.Through in vitro experiments,cell viability test showed that WFA protected SH-SY5Y neuronal cells against hemin-induced cell injury.Enzyme-linked immunosorbent assays in vitro and in vivo showed that WFA markedly decreased the level of malondialdehyde,an oxidative stress marker,and increased the activities of anti-oxidative stress markers superoxide dismutase and glutathione peroxidase after ICH.Western blot assay,quantitative polymerase chain reaction and immunofluorescence results demonstrated that WFA activated the nuclear factor E2-related factor 2(Nrf2)/heme oxygenase-1(HO-1)signaling axis,promoted translocation of Nrf2 from the cytoplasm to nucleus,and increased HO-1 expression.Silencing Nrf2 with siRNA completely reversed HO-1 expression,oxidative stress and protective effects of WFA.Furthermore,WFA reduced hemin-induced ferroptosis.However,after treatment with an HO-1 inhibitor,the neuroprotective effects of WFA against hemin-induced ferroptosis were weakened.MTT test results showed that WFA combined with ferrostatin-1 reduced hemin-induced SH-SY5Y neuronal cell injury.Our findings reveal that WFA treatment alleviated ICH injury-induced ferroptosis and oxidative stress through activating the Nrf2/HO-1 pathway,which may highlight a potential role of WFA for the treatment of ICH.展开更多
Currently,some photorealistic computer graphics are very similar to photographic images.Photorealistic computer generated graphics can be forged as photographic images,causing serious security problems.The aim of this...Currently,some photorealistic computer graphics are very similar to photographic images.Photorealistic computer generated graphics can be forged as photographic images,causing serious security problems.The aim of this work is to use a deep neural network to detect photographic images(PI)versus computer generated graphics(CG).In existing approaches,image feature classification is computationally intensive and fails to achieve realtime analysis.This paper presents an effective approach to automatically identify PI and CG based on deep convolutional neural networks(DCNNs).Compared with some existing methods,the proposed method achieves real-time forensic tasks by deepening the network structure.Experimental results show that this approach can effectively identify PI and CG with average detection accuracy of 98%.展开更多
Secondary degeneration occurs commonly in the central nervous system after traumatic injuries and following acute and chronic diseases, including glaucoma. A constellation of mechanisms have been shown to be associate...Secondary degeneration occurs commonly in the central nervous system after traumatic injuries and following acute and chronic diseases, including glaucoma. A constellation of mechanisms have been shown to be associated with secondary degeneration including apoptosis, necrosis, autophagy, oxidative stress, excitotoxicity, derangements in ionic homeostasis and calcium influx. Glial cells, such as microglia, astrocytes and oligodendrocytes, have also been demon- strated to take part in the process of secondary injury. Partial optic nerve transection is a useful model which was established about 13 years ago. The merit of this model compared with other optic nerve injury models used for glaucoma study, including complete optic nerve transection model and optic nerve crush model, is the possibility to separate primary degeneration from secondary degeneration in location. Therefore, it provides a good tool for the study of secondary degeneration. This review will focus on the research progress of the mechanisms of secondary degeneration using partial optic nerve transection model.展开更多
Aluminum foils having thicknesses of 10-20 μm are commonly employed as current collectors for cathode electrodes in Li-ion batteries. The effects of the surface morphology of the foil on battery performance were inve...Aluminum foils having thicknesses of 10-20 μm are commonly employed as current collectors for cathode electrodes in Li-ion batteries. The effects of the surface morphology of the foil on battery performance were investigated by using a foil with roughened surface by chemical etching and a plain foil with smooth surface on both sides. For high-conductivity LiCoO2 active materials with large particle size, there are no significant differences in battery performance between the two types of foils. But for low-conductivity LiFePO4 active materials with small particle size, high-rate discharge properties are significantly different. The possibility shows that optimizing both the surface morphology of the aluminum foil and particle size of active material leads to improvement of the battery performance.展开更多
Soil spatial variability is difficult to evaluate due to insufficient test data.An alternative option is estimation by indirect methods such as inverse analysis.In this paper,two examples are presented to demonstrate ...Soil spatial variability is difficult to evaluate due to insufficient test data.An alternative option is estimation by indirect methods such as inverse analysis.In this paper,two examples are presented to demonstrate the capability and accuracy of the probabilistic estimation method to characterize soil spatial variability with displacement responses.The first example is a soil slope subject to a surcharge load,in which the spatially varied field of the elastic modulus is estimated with displacements.The results show that estimations based on horizontal displacements were more accurate than those based on vertical displacements.The accuracy of the estimated field was substantially reduced by increasing variance of elastic modulus.However,the estimation was generally acceptable as the error was not more than 10%,even for the high variance case(COV^l.5).The accuracy of estimation was also affected by the type of covariance function and the correlation length.When the correlation length decreased,the accuracy of estimation was reduced.The second example is a validation of laboratory model tests where a horizontal load was applied on a layered ground.The estimated thicknesses of soil layers were close to those in the real situation,which demonstrates the capacity of the estimation method.展开更多
With the development of data science and technology,information security has been further concerned.In order to solve privacy problems such as personal privacy being peeped and copyright being infringed,information hi...With the development of data science and technology,information security has been further concerned.In order to solve privacy problems such as personal privacy being peeped and copyright being infringed,information hiding algorithms has been developed.Image information hiding is to make use of the redundancy of the cover image to hide secret information in it.Ensuring that the stego image cannot be distinguished from the cover image,and sending secret information to receiver through the transmission of the stego image.At present,the model based on deep learning is also widely applied to the field of information hiding.This paper makes an overall conclusion on image information hiding based on deep learning.It is divided into four parts of steganography algorithms,watermarking embedding algorithms,coverless information hiding algorithms and steganalysis algorithms based on deep learning.From these four aspects,the state-of-the-art information hiding technologies based on deep learning are illustrated and analyzed.展开更多
Gliomas are the most commonly occurring tumors of the central nervous system. Glioblastoma multiforme (GBM) is the most malignant and aggressive brain cancer in adults. Further understanding of the mechanisms underlyi...Gliomas are the most commonly occurring tumors of the central nervous system. Glioblastoma multiforme (GBM) is the most malignant and aggressive brain cancer in adults. Further understanding of the mechanisms underlying the aggressive nature of GBM is urgently needed. Here we identified homeobox B8(HOXB8), a member of the homeobox family, as a crucial contributor to the aggressiveness of GBM. Data mining of publicly accessible RNA sequence datasets and our patient cohorts confirmed a higher expression of HOXB8 in the tumor tissue of GBM patients, and a strong positive correlation between the expression level and pathological grading of tumors and a negative correlation between the expression level and the overall survival rate. We next showed that HOXB8 promotes the proliferation and migration of glioblastoma cells and is crucial for the activation of the PI3K/AKT pathway and expression of epithelial–mesenchymal transition-related genes, possibly through direct binding to the promoter of SAMD9 (Sterile Alpha Motif Domain-Containing Protein 9) and activating its transcription. Collectively, we identified HOXB8 as a critical contributor to the aggressiveness of GBM, which provides insights into a potential therapeutic target for GBM and opens new avenues for improving its treatment outcome.展开更多
Background and aim:Transjugular intrahepatic portosystemic shunt(TIPS)is a technique successfully used to treat portal hypertension and its complications.However,the choice of the branch,left(L)or right(R),of the port...Background and aim:Transjugular intrahepatic portosystemic shunt(TIPS)is a technique successfully used to treat portal hypertension and its complications.However,the choice of the branch,left(L)or right(R),of the portal vein resulting in a better outcome is still under debate.Therefore,this meta-analysis aims to evaluate which branch has a better curative effect on patients treated with TIPS.Methods:PubMed,EMBASE,Web of science,Cochrane Library databases,Wanfang database and CBM were used for our search in October 2019 and updated in June 2021.The following parameters were used in evaluation:overall mortality,hepatic encephalopathy,shunt dysfunction,variceal rebleeding and rate of postoperative ascites.Results:There were seven studies included.The sample size was 1940.A lower risk of mortality was observed in TIPS-L-treated patients compared with TIPS-R-treated ones(OR=0.65,95%CI=0.50-0.85,p=0.002).A lower risk of shunt dysfunction was observed in TIPS-L-treated patients compared with TIPS-R-treated ones(OR=0.53,95%CI=0.33-0.87,p=0.01).And the TIPS-L group had a significantly higher hepatic encephalopathy-free rate than the TIPS-R group(OR=0.59,95%CI=0.44-0.78,p=0.0002).However,the rate of rebleeding(OR=0.75,95%CI=0.55-1.03,p=0.07)and incidence of postoperative ascites(OR=1.14,95%CI=0.86-1.51,p=0.38)was not statistically significant between the two groups.Conclusions:Based on the currently available evidence,the technique of TIPS through the left branch of the portal vein can significantly reduce the occurrence of overall postoperative mortality,hepatic encephalopathy and shunt dysfunction.展开更多
At present,the coverless information hiding has been developed.However,due to the limited mapping relationship between secret information and feature selection,it is challenging to further enhance the hiding capacity ...At present,the coverless information hiding has been developed.However,due to the limited mapping relationship between secret information and feature selection,it is challenging to further enhance the hiding capacity of coverless information hiding.At the same time,the steganography algorithm based on object detection only hides secret information in foreground objects,which contribute to the steganography capacity is reduced.Since object recognition contains multiple objects and location,secret information can be mapped to object categories,the relationship of location and so on.Therefore,this paper proposes a new steganography algorithm based on object detection and relationship mapping,which integrates coverless information hiding and steganography.In this method,the coverless information hiding is realized by mapping the object type,color and secret information in object detection method.At the same time,the object detection method is used to find the safe area to hide secret messages.The proposed algorithm can not only improve the steganographic capacity of the two information hiding methods but also make the coverless information hiding more secure and robust.展开更多
Injury to axons close to the neuronal bodies in the mammalian central nervous system causes a large proportion of parenting neurons to degenerate. It is known that optic nerve transection close to the eye in rodents l...Injury to axons close to the neuronal bodies in the mammalian central nervous system causes a large proportion of parenting neurons to degenerate. It is known that optic nerve transection close to the eye in rodents leads to a loss of about half of retinal ganglion cells in 1 week and about 90% in 2 weeks. Using low level laser treatment in the present study, we demonstrated that treatment with helium-neon （660 nm） laser with 15 mW power could delay retinal ganglion cell death after optic nerve axotomy in adult hamsters. The effect was most apparent in the ifrst week with a short period of treatment time （5 minutes） in which 65–66% of retinal ganglion cells survived the optic nerve axotomy whereas 45–47% of retinal ganglion cells did so in optic nerve axotomy controls. We also found that single dose and early commencement of laser irradiation were important in protecting retinal ganglion cells following optic nerve axotomy. These ifndings thus convincingly show that appropriate laser treatment may be neuroprotective to retinal gan-glion cells.展开更多
Intraocular pressure elevation can induce retinal ganglion cell death and is a clinically reversible risk factor for glaucoma,the leading cause of irreversible blindness.We previously demonstrated that casein kinase-2...Intraocular pressure elevation can induce retinal ganglion cell death and is a clinically reversible risk factor for glaucoma,the leading cause of irreversible blindness.We previously demonstrated that casein kinase-2 inhibition can promote retinal ganglion cell survival and axonal regeneration in rats after optic nerve injury.To investigate the underlying mechanism,in the current study we increased the intraocular pressure of adult rats to 75 mmHg for 2 hours and then administered a casein kinase-2 inhibitor(4,5,6,7-tetrabromo-2-azabenzimidazole or 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole)by intravitreal injection.We found that intravitreal injection of 4,5,6,7-tetrabromo-2-azabenzimidazole or 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole promoted retinal ganglion cell survival and reduced the number of infiltrating macrophages.Transcriptomic analysis showed that the mitogen activated protein kinase signaling pathway was involved in the response to intraocular pressure elevation but was not modulated by the casein kinase-2 inhibitors.Furthermore,casein kinase-2 inhibition downregulated the expression of genes(Cck,Htrsa,Nef1,Htrlb,Prph,Chat,Slc18a3,Slc5a7,Scn1b,Crybb2,Tsga10ip,and Vstm21)involved in intraocular pressure elevation.Our data indicate that inhibition of casein kinase-2 can enhance retinal ganglion cell survival in rats after acute intraocular pressure elevation via macrophage inactivation.展开更多
Information hiding tends to hide secret information in image area where is rich texture or high frequency,so as to transmit secret information to the recipient without affecting the visual quality of the image and aro...Information hiding tends to hide secret information in image area where is rich texture or high frequency,so as to transmit secret information to the recipient without affecting the visual quality of the image and arousing suspicion.We take advantage of the complexity of the object texture and consider that under certain circumstances,the object texture is more complex than the background of the image,so the foreground object is more suitable for steganography than the background.On the basis of instance segmentation,such as Mask R-CNN,the proposed method hides secret information into each object's region by using the masks of instance segmentation,thus realizing the information hiding of the foreground object without background.This method not only makes it more efficient for the receiver to extract information,but also proves to be more secure and robust by experiments.展开更多
BACKGROUND For cases of middle and low biliary obstruction with left and right hepatic duct dilatation,the type of approach and whether different approaches affect the difficulty of puncture operation and intraoperati...BACKGROUND For cases of middle and low biliary obstruction with left and right hepatic duct dilatation,the type of approach and whether different approaches affect the difficulty of puncture operation and intraoperative and postoperative complications have not been discussed in detail.AIM To compare the efficacy of different percutaneous transhepatic biliary stent placements and catheter drainage in treating middle and low biliary obstruction.METHODS A retrospective analysis was performed on the medical records of 424 patients with middle and low biliary obstruction who underwent percutaneous liver puncture biliary stent placement and catheter drainage at the Department of Interventional Radiology,Shaanxi Provincial People’s Hospital between March 2016 and March 2022.Based on the puncture path,patients were categorized into two groups:Subxiphoid left hepatic lobe approach group(Group A,224 cases)and right intercostal,right hepatic lobe approach group(Group B,200 cases).Liver function improvement,postoperative biliary bleeding incidence,postoperative pain duration,and abdominal effusion leakage around the drainage tube were compared between the two groups at 3 d and 1 wk after the surgery.Patient survival time was recorded during follow-up.RESULTS All 424 surgeries were successful without adverse events.Group A comprised 224 cases,and Group B had 200 cases.There was no statistically significant difference in basic data between Group A and Group B(P>0.05).No significant difference in postoperative biliary bleeding incidence was observed between the groups(P>0.05).The decreased rates for total bilirubin(Group A:69.23±4.50,Group B:63.79±5.65),direct bilirubin(Group A:79.30±11.19,Group B:63.62±5.64),and alkaline phosphatase(Group A:60.51±12.23,Group B:42.68±23.56)in the 1st wk after surgery were significantly faster in Group A than in Group B.The decreased rate of gamma-glutamyl transpeptidase was also significantly faster in Group A at both 3 d(Group A:40.56±10.32,Group B:32.22±5.12)and 1 wk(Group A:73.19±7.05,Group B:58.81±18.98)after surgery(P<0.05).Group A experienced significantly less peritoneal effusion leakage around the drainage tube than Group B(P<0.05).The patient survival rate was higher in Group A compared to Group B(P<0.05).CONCLUSION In treating jaundice patients with middle and low biliary obstruction,a percutaneous left liver puncture demonstrated better clinical efficacy than a percutaneous right liver puncture.展开更多
Flavonoids are the primary functional components in the flowers of Hibiscus manihot L.(HMLF).In this study,an efficient and green ionic liquid-high-speed homogenization coupled with microwave-assisted extraction(IL-HS...Flavonoids are the primary functional components in the flowers of Hibiscus manihot L.(HMLF).In this study,an efficient and green ionic liquid-high-speed homogenization coupled with microwave-assisted extraction(IL-HSH-MAE)technique was firstly established and implemented to extract seven target flavonoids from HMLF.Single-factor experiments and Box-Behnken design(BBD)were utilized to maximize the extraction conditions of IL-HSH-MAE,which were as follows:0.1 M of[C4mim]Br,homogenate speed of 7,000 rpm,homogenate time of 120 s,liquid/solid ratio of 24 mL/g,extraction temperature of 62℃and extraction time of 15 min.The maximal total extraction yield of seven target flavonoids attained 22.04 mg/g,which was considerably greater than the yields obtained by IL-HSH,IL-MAE,60%ethanol-HSH-MAE and 60%ethanol-MAE.These findings suggested that the IL-HSH-MAE method can be exploited as a rapid and efficient approach for extracting natural products from plants.The process also possesses outstanding superiority in being environmentally friendly and having a high extraction efficiency and is expected to be a luciferous prospect extraction technology.展开更多
基金supported by the National Natural Science Foundation of China(grant number 31571892)National Natural Science Fund of China(grant number 82204552)+1 种基金Natural Science Foundation of Zhejiang Province(grant number LQ22H280007)Research Project of Zhejiang Chinese Medical University(grant number 2022JKZKTS10).
文摘In order to investigate the extraction efficiency of flavonoids by octenyl succinic acid-modified starch(OSA-modified starch)with varying molecular weights,the immature fruit of Citrus paradisi'Changshan Huyou',commonly known as"Quzhiqiao",was used as the extraction target in this research.OSA-modified starch was successfully identified and refined,ensuring its molecular weight was appropriate and extraction was optimized.The experimental findings suggested that a concentration of 20 mg/mL of medium molecular weight gelatinized OSA-modified starch was optimal for extracting flavonoids from"Quzhiqiao"using an ultrasound-assisted method(1,000 W,40 min).Additionally,the ratio of the mass of"Quzhiqiao"to the volume of gelatinized OSA-modified starch employed in this process was determined to be 15 mg/mL.The findings from the in vitro antioxidant studies indicated significant variations in the antioxidant activities of the extract solutions of OSA-modified starches with varying molecular weights.These differences can potentially be attributed to substantial variations in the extract solutions composition.
基金supported by the Chief Scientist of Qi-Huang Project of the National Traditional Chinese Medicine Inheritance and Innovation“One Hundred Million”Talent Project,China(Grant No.:[2021]No.7)the National Famous Old Traditional Chinese Medicine Experts Inheritance Studio Construction Program of National Administration of Traditional Chinese Medicine,China(Grant No.:[2022]No.75)+3 种基金the Seventh Batch of National Famous Old Traditional Chinese Medicine Experts Experience Heritage Construction Program of National Administration of Traditional Chinese Medicine,China(Grant No.:[2022]No.76)Heilongjiang Touyan Innovation Team Program,China(Grant No.:[2019]No.5)the Natural Science Foundation of Zhejiang Province(Grant No.:LQN25H280009)the Research Project of Zhejiang Chinese Medical University,China(Grant No.:2023RCZXZK22).
文摘That herbs with the"hot"property used to treat"cold"syndromes is a guiding principle of clinical prescription and medication in traditional Chinese medicine(TCM).However,this theory of TCM is still in the‘black box'stage,and few in-depth studies have examined the biological mechanisms underpinning the hot properties of herbs.
基金supported by the Natural Science Foundation of Guangxi Zhuang Autonomous Region,No.2020GXNSFAA259036(to RJL)the Guangxi Science and Technology Project,No.Guike AD17129015(to QHL)+1 种基金Guangxi Research and Innovation Base for Basic and Clinical Application of Nerve Injury and Repair Project,No.Guike ZY21195042(to QHL)the Innovation Projects of Guangxi Graduate Education,Nos.YCSW2021246(to ZXZ),YCSW2021254(to WJX).
文摘Recent studies have indicated that suppressing oxidative stress and ferroptosis can considerably improve the prognosis of intracerebral hemorrhage(ICH).Withaferin A(WFA),a natural compound,exhibits a positive effect on a number of neurological diseases.However,the effects of WFA on oxidative stress and ferroptosis-mediated signaling pathways to ICH remain unknown.In this study,we investigated the neuroprotective effects and underlying mechanism for WFA in the regulation of ICH-induced oxidative stress and ferroptosis.We established a mouse model of ICH by injection of autologous tail artery blood into the caudate nucleus and an in vitro cell model of hemin-induced ICH.WFA was injected intracerebroventricularly at 0.1,1 or 5μg/kg once daily for 7 days,starting immediately after ICH operation.WFA markedly reduced brain tissue injury and iron deposition and improved neurological function in a dose-dependent manner 7 days after cerebral hemorrhage.Through in vitro experiments,cell viability test showed that WFA protected SH-SY5Y neuronal cells against hemin-induced cell injury.Enzyme-linked immunosorbent assays in vitro and in vivo showed that WFA markedly decreased the level of malondialdehyde,an oxidative stress marker,and increased the activities of anti-oxidative stress markers superoxide dismutase and glutathione peroxidase after ICH.Western blot assay,quantitative polymerase chain reaction and immunofluorescence results demonstrated that WFA activated the nuclear factor E2-related factor 2(Nrf2)/heme oxygenase-1(HO-1)signaling axis,promoted translocation of Nrf2 from the cytoplasm to nucleus,and increased HO-1 expression.Silencing Nrf2 with siRNA completely reversed HO-1 expression,oxidative stress and protective effects of WFA.Furthermore,WFA reduced hemin-induced ferroptosis.However,after treatment with an HO-1 inhibitor,the neuroprotective effects of WFA against hemin-induced ferroptosis were weakened.MTT test results showed that WFA combined with ferrostatin-1 reduced hemin-induced SH-SY5Y neuronal cell injury.Our findings reveal that WFA treatment alleviated ICH injury-induced ferroptosis and oxidative stress through activating the Nrf2/HO-1 pathway,which may highlight a potential role of WFA for the treatment of ICH.
基金This work is supported,in part,by the National Natural Science Foundation of China under grant numbers U1536206,U1405254,61772283,61602253,61672294,61502242In part,by the Jiangsu Basic Research Programs-Natural Science Foundation under grant numbers BK20150925 and BK20151530+1 种基金In part,by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)fundIn part,by the Collaborative Innovation Center of Atmospheric Environment and Equipment Technology(CICAEET)fund,China.
文摘Currently,some photorealistic computer graphics are very similar to photographic images.Photorealistic computer generated graphics can be forged as photographic images,causing serious security problems.The aim of this work is to use a deep neural network to detect photographic images(PI)versus computer generated graphics(CG).In existing approaches,image feature classification is computationally intensive and fails to achieve realtime analysis.This paper presents an effective approach to automatically identify PI and CG based on deep convolutional neural networks(DCNNs).Compared with some existing methods,the proposed method achieves real-time forensic tasks by deepening the network structure.Experimental results show that this approach can effectively identify PI and CG with average detection accuracy of 98%.
基金supported by a grant from the Research Grants Council of the Hong Kong Special Administrative Region,China(HKU 776109M)supported by the Fundamental Research Funds for the Central Universities Grant 21609101
文摘Secondary degeneration occurs commonly in the central nervous system after traumatic injuries and following acute and chronic diseases, including glaucoma. A constellation of mechanisms have been shown to be associated with secondary degeneration including apoptosis, necrosis, autophagy, oxidative stress, excitotoxicity, derangements in ionic homeostasis and calcium influx. Glial cells, such as microglia, astrocytes and oligodendrocytes, have also been demon- strated to take part in the process of secondary injury. Partial optic nerve transection is a useful model which was established about 13 years ago. The merit of this model compared with other optic nerve injury models used for glaucoma study, including complete optic nerve transection model and optic nerve crush model, is the possibility to separate primary degeneration from secondary degeneration in location. Therefore, it provides a good tool for the study of secondary degeneration. This review will focus on the research progress of the mechanisms of secondary degeneration using partial optic nerve transection model.
文摘Aluminum foils having thicknesses of 10-20 μm are commonly employed as current collectors for cathode electrodes in Li-ion batteries. The effects of the surface morphology of the foil on battery performance were investigated by using a foil with roughened surface by chemical etching and a plain foil with smooth surface on both sides. For high-conductivity LiCoO2 active materials with large particle size, there are no significant differences in battery performance between the two types of foils. But for low-conductivity LiFePO4 active materials with small particle size, high-rate discharge properties are significantly different. The possibility shows that optimizing both the surface morphology of the aluminum foil and particle size of active material leads to improvement of the battery performance.
基金the National Natural Science Foundation of China(Nos.51979158,51639008,51679135,and 51422905)the Program of Shanghai Academic Research Leader(No.19XD1421900),China。
文摘Soil spatial variability is difficult to evaluate due to insufficient test data.An alternative option is estimation by indirect methods such as inverse analysis.In this paper,two examples are presented to demonstrate the capability and accuracy of the probabilistic estimation method to characterize soil spatial variability with displacement responses.The first example is a soil slope subject to a surcharge load,in which the spatially varied field of the elastic modulus is estimated with displacements.The results show that estimations based on horizontal displacements were more accurate than those based on vertical displacements.The accuracy of the estimated field was substantially reduced by increasing variance of elastic modulus.However,the estimation was generally acceptable as the error was not more than 10%,even for the high variance case(COV^l.5).The accuracy of estimation was also affected by the type of covariance function and the correlation length.When the correlation length decreased,the accuracy of estimation was reduced.The second example is a validation of laboratory model tests where a horizontal load was applied on a layered ground.The estimated thicknesses of soil layers were close to those in the real situation,which demonstrates the capacity of the estimation method.
基金This work is supported by the National Key R&D Program of China under grant 2018YFB1003205by the National Natural Science Foundation of China under grant U1836208,U1536206,U1836110,61602253,61672294+2 种基金by the Jiangsu Basic Research Programs-Natural Science Foundation under grant numbers BK20181407by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAP-D)fundby the Collaborative Innovation Center of Atmospheric Environment and Equipment Technology(CICAEET)fund,China。
文摘With the development of data science and technology,information security has been further concerned.In order to solve privacy problems such as personal privacy being peeped and copyright being infringed,information hiding algorithms has been developed.Image information hiding is to make use of the redundancy of the cover image to hide secret information in it.Ensuring that the stego image cannot be distinguished from the cover image,and sending secret information to receiver through the transmission of the stego image.At present,the model based on deep learning is also widely applied to the field of information hiding.This paper makes an overall conclusion on image information hiding based on deep learning.It is divided into four parts of steganography algorithms,watermarking embedding algorithms,coverless information hiding algorithms and steganalysis algorithms based on deep learning.From these four aspects,the state-of-the-art information hiding technologies based on deep learning are illustrated and analyzed.
基金the National Natural Science Foundation of China(31571298).
文摘Gliomas are the most commonly occurring tumors of the central nervous system. Glioblastoma multiforme (GBM) is the most malignant and aggressive brain cancer in adults. Further understanding of the mechanisms underlying the aggressive nature of GBM is urgently needed. Here we identified homeobox B8(HOXB8), a member of the homeobox family, as a crucial contributor to the aggressiveness of GBM. Data mining of publicly accessible RNA sequence datasets and our patient cohorts confirmed a higher expression of HOXB8 in the tumor tissue of GBM patients, and a strong positive correlation between the expression level and pathological grading of tumors and a negative correlation between the expression level and the overall survival rate. We next showed that HOXB8 promotes the proliferation and migration of glioblastoma cells and is crucial for the activation of the PI3K/AKT pathway and expression of epithelial–mesenchymal transition-related genes, possibly through direct binding to the promoter of SAMD9 (Sterile Alpha Motif Domain-Containing Protein 9) and activating its transcription. Collectively, we identified HOXB8 as a critical contributor to the aggressiveness of GBM, which provides insights into a potential therapeutic target for GBM and opens new avenues for improving its treatment outcome.
基金Key Laboratory of Molecular Diagnostics and Precision Medicine for Surgical Oncology in Gansu Province(18JR2RA033)。
文摘Background and aim:Transjugular intrahepatic portosystemic shunt(TIPS)is a technique successfully used to treat portal hypertension and its complications.However,the choice of the branch,left(L)or right(R),of the portal vein resulting in a better outcome is still under debate.Therefore,this meta-analysis aims to evaluate which branch has a better curative effect on patients treated with TIPS.Methods:PubMed,EMBASE,Web of science,Cochrane Library databases,Wanfang database and CBM were used for our search in October 2019 and updated in June 2021.The following parameters were used in evaluation:overall mortality,hepatic encephalopathy,shunt dysfunction,variceal rebleeding and rate of postoperative ascites.Results:There were seven studies included.The sample size was 1940.A lower risk of mortality was observed in TIPS-L-treated patients compared with TIPS-R-treated ones(OR=0.65,95%CI=0.50-0.85,p=0.002).A lower risk of shunt dysfunction was observed in TIPS-L-treated patients compared with TIPS-R-treated ones(OR=0.53,95%CI=0.33-0.87,p=0.01).And the TIPS-L group had a significantly higher hepatic encephalopathy-free rate than the TIPS-R group(OR=0.59,95%CI=0.44-0.78,p=0.0002).However,the rate of rebleeding(OR=0.75,95%CI=0.55-1.03,p=0.07)and incidence of postoperative ascites(OR=1.14,95%CI=0.86-1.51,p=0.38)was not statistically significant between the two groups.Conclusions:Based on the currently available evidence,the technique of TIPS through the left branch of the portal vein can significantly reduce the occurrence of overall postoperative mortality,hepatic encephalopathy and shunt dysfunction.
基金the National Key R&D Program of China under grant 2018YFB1003205by the National Natural Science Foundation of China under grant U1836208,U1536206,U1836110,61602253,61672294+2 种基金by the Jiangsu Basic Research Programs-Natural Science Foundation under grant numbers BK20181407by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)fundby the Collaborative Innovation Center of Atmospheric Environment and Equipment Technology(CICAEET)fund,China.
文摘At present,the coverless information hiding has been developed.However,due to the limited mapping relationship between secret information and feature selection,it is challenging to further enhance the hiding capacity of coverless information hiding.At the same time,the steganography algorithm based on object detection only hides secret information in foreground objects,which contribute to the steganography capacity is reduced.Since object recognition contains multiple objects and location,secret information can be mapped to object categories,the relationship of location and so on.Therefore,this paper proposes a new steganography algorithm based on object detection and relationship mapping,which integrates coverless information hiding and steganography.In this method,the coverless information hiding is realized by mapping the object type,color and secret information in object detection method.At the same time,the object detection method is used to find the safe area to hide secret messages.The proposed algorithm can not only improve the steganographic capacity of the two information hiding methods but also make the coverless information hiding more secure and robust.
基金supported by Key Basic Research Project of China(973 Program),No.2011CB707501Fundamental Research Funds for The Central Universities,No.21609101
文摘Injury to axons close to the neuronal bodies in the mammalian central nervous system causes a large proportion of parenting neurons to degenerate. It is known that optic nerve transection close to the eye in rodents leads to a loss of about half of retinal ganglion cells in 1 week and about 90% in 2 weeks. Using low level laser treatment in the present study, we demonstrated that treatment with helium-neon （660 nm） laser with 15 mW power could delay retinal ganglion cell death after optic nerve axotomy in adult hamsters. The effect was most apparent in the ifrst week with a short period of treatment time （5 minutes） in which 65–66% of retinal ganglion cells survived the optic nerve axotomy whereas 45–47% of retinal ganglion cells did so in optic nerve axotomy controls. We also found that single dose and early commencement of laser irradiation were important in protecting retinal ganglion cells following optic nerve axotomy. These ifndings thus convincingly show that appropriate laser treatment may be neuroprotective to retinal gan-glion cells.
基金supported by the National Natural Science Foundation of China,Nos.81570849,81100931the Natural Science Foundation of Guangdong Province of China,Nos.2015A030313446,2020A1515011413(all to LPC).
文摘Intraocular pressure elevation can induce retinal ganglion cell death and is a clinically reversible risk factor for glaucoma,the leading cause of irreversible blindness.We previously demonstrated that casein kinase-2 inhibition can promote retinal ganglion cell survival and axonal regeneration in rats after optic nerve injury.To investigate the underlying mechanism,in the current study we increased the intraocular pressure of adult rats to 75 mmHg for 2 hours and then administered a casein kinase-2 inhibitor(4,5,6,7-tetrabromo-2-azabenzimidazole or 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole)by intravitreal injection.We found that intravitreal injection of 4,5,6,7-tetrabromo-2-azabenzimidazole or 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole promoted retinal ganglion cell survival and reduced the number of infiltrating macrophages.Transcriptomic analysis showed that the mitogen activated protein kinase signaling pathway was involved in the response to intraocular pressure elevation but was not modulated by the casein kinase-2 inhibitors.Furthermore,casein kinase-2 inhibition downregulated the expression of genes(Cck,Htrsa,Nef1,Htrlb,Prph,Chat,Slc18a3,Slc5a7,Scn1b,Crybb2,Tsga10ip,and Vstm21)involved in intraocular pressure elevation.Our data indicate that inhibition of casein kinase-2 can enhance retinal ganglion cell survival in rats after acute intraocular pressure elevation via macrophage inactivation.
基金This work is supported by the National Key R&D Program of China under grant 2018YFB1003205by the National Natural Science Foundation of China under grant U1836208,U1536206,U1836110,61602253,61672294+2 种基金by the Jiangsu Basic Research Programs-Natural Science Foundation under grant numbers BK20181407by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)fundby the Collaborative Innovation Center of Atmospheric Environment and Equipment Technology(CICAEET)fund,China.
文摘Information hiding tends to hide secret information in image area where is rich texture or high frequency,so as to transmit secret information to the recipient without affecting the visual quality of the image and arousing suspicion.We take advantage of the complexity of the object texture and consider that under certain circumstances,the object texture is more complex than the background of the image,so the foreground object is more suitable for steganography than the background.On the basis of instance segmentation,such as Mask R-CNN,the proposed method hides secret information into each object's region by using the masks of instance segmentation,thus realizing the information hiding of the foreground object without background.This method not only makes it more efficient for the receiver to extract information,but also proves to be more secure and robust by experiments.
文摘BACKGROUND For cases of middle and low biliary obstruction with left and right hepatic duct dilatation,the type of approach and whether different approaches affect the difficulty of puncture operation and intraoperative and postoperative complications have not been discussed in detail.AIM To compare the efficacy of different percutaneous transhepatic biliary stent placements and catheter drainage in treating middle and low biliary obstruction.METHODS A retrospective analysis was performed on the medical records of 424 patients with middle and low biliary obstruction who underwent percutaneous liver puncture biliary stent placement and catheter drainage at the Department of Interventional Radiology,Shaanxi Provincial People’s Hospital between March 2016 and March 2022.Based on the puncture path,patients were categorized into two groups:Subxiphoid left hepatic lobe approach group(Group A,224 cases)and right intercostal,right hepatic lobe approach group(Group B,200 cases).Liver function improvement,postoperative biliary bleeding incidence,postoperative pain duration,and abdominal effusion leakage around the drainage tube were compared between the two groups at 3 d and 1 wk after the surgery.Patient survival time was recorded during follow-up.RESULTS All 424 surgeries were successful without adverse events.Group A comprised 224 cases,and Group B had 200 cases.There was no statistically significant difference in basic data between Group A and Group B(P>0.05).No significant difference in postoperative biliary bleeding incidence was observed between the groups(P>0.05).The decreased rates for total bilirubin(Group A:69.23±4.50,Group B:63.79±5.65),direct bilirubin(Group A:79.30±11.19,Group B:63.62±5.64),and alkaline phosphatase(Group A:60.51±12.23,Group B:42.68±23.56)in the 1st wk after surgery were significantly faster in Group A than in Group B.The decreased rate of gamma-glutamyl transpeptidase was also significantly faster in Group A at both 3 d(Group A:40.56±10.32,Group B:32.22±5.12)and 1 wk(Group A:73.19±7.05,Group B:58.81±18.98)after surgery(P<0.05).Group A experienced significantly less peritoneal effusion leakage around the drainage tube than Group B(P<0.05).The patient survival rate was higher in Group A compared to Group B(P<0.05).CONCLUSION In treating jaundice patients with middle and low biliary obstruction,a percutaneous left liver puncture demonstrated better clinical efficacy than a percutaneous right liver puncture.
基金support from China Postdoctoral Science Foundation(2021M692893,2021M702927)National Natural Science Fund of China(82204552)+2 种基金Natural Science Foundation of Zhejiang Province(LQ22H280007)Research Project of Zhejiang Chinese Medical University(2022JKZKTS10)Zhejiang Province Traditional Chinese Medicine Science and Technology(2023ZR079,2023ZR087).
文摘Flavonoids are the primary functional components in the flowers of Hibiscus manihot L.(HMLF).In this study,an efficient and green ionic liquid-high-speed homogenization coupled with microwave-assisted extraction(IL-HSH-MAE)technique was firstly established and implemented to extract seven target flavonoids from HMLF.Single-factor experiments and Box-Behnken design(BBD)were utilized to maximize the extraction conditions of IL-HSH-MAE,which were as follows:0.1 M of[C4mim]Br,homogenate speed of 7,000 rpm,homogenate time of 120 s,liquid/solid ratio of 24 mL/g,extraction temperature of 62℃and extraction time of 15 min.The maximal total extraction yield of seven target flavonoids attained 22.04 mg/g,which was considerably greater than the yields obtained by IL-HSH,IL-MAE,60%ethanol-HSH-MAE and 60%ethanol-MAE.These findings suggested that the IL-HSH-MAE method can be exploited as a rapid and efficient approach for extracting natural products from plants.The process also possesses outstanding superiority in being environmentally friendly and having a high extraction efficiency and is expected to be a luciferous prospect extraction technology.
