Background and Objective: Syzygium aromaticum is an immunomodulator with the main active compound eugenol which can stimulate the function of lymphocyte proliferation and macrophage production. Lymphocytes play an imp...Background and Objective: Syzygium aromaticum is an immunomodulator with the main active compound eugenol which can stimulate the function of lymphocyte proliferation and macrophage production. Lymphocytes play an important role for body protection against infection. This study aims to analyze the effect of S. aromaticum extract to increase proliferation of lymphocytes, lymphoblasts and macrophage production in Balb/c mice infected with Salmonella typhimurium. Methodology: Mice strain Balb/c were divided into 4 groups: treatment group infected with S. typhimurium and control group (without treatment) to tested the activity of peritoneal macrophages. The treatment group was administrated by S. aromaticum leaf extract (15 mg, 75 mg, 150 mg/kgbw) for 12 days. Results: S. aromaticum leaf extract increased the proliferation activity (p Conclusion: Administration of S. aromaticum leaf extract increased the proliferation activity of lymphocytes, lymphoblasts and ROI secretion of macrophagesin Balb/c mice infected with S. typhimurium.展开更多
Backround: Erectile dysfunction (ED) is the inability of male reproductive organs within sexual intercourse caused by neurogenic and hormonal disorders. There are some causes of ED such as hypertension, stress, neurol...Backround: Erectile dysfunction (ED) is the inability of male reproductive organs within sexual intercourse caused by neurogenic and hormonal disorders. There are some causes of ED such as hypertension, stress, neurological disorders, stroke, diabetes, atherosclerosis, lifestyle, alcohol, smoking, and age-related hormonal decline can cause infertility. The natural treatment of sexual dysfunction through aphrodisiac activity of the plant is to increase sexual hormones, spermatogenesis activity and through PDE5 inhibitors such as sildenafil, verdenafil, and tadalafil which can inhibit the hydrolysis of second messenger cGMP of penis smooth muscle cells. Primer Design for amplification of several PDE5 gene nucleotide sequences obtained from NCBI gen banks and tested directly through explosions at NCBI and also using MEGA 6, primer 3 plus, and fast PCR software. The natural treatment of sexual dysfunction through aphrodisiac activity from plants to increase sexual hormone, spermatogenesis activity and inhibitor PDE5 such as sildenafil, vardenafil, and tadalafil can inhibit hydrolysis second messenger of cells cGMP smooth penis muscles. Method: Primer design stages for several sequences are data supply, multiple sequence alignment, sequence trimming, primer design (fastPCR input), in silico PCR analysis, and primer evaluation (Primer Test, OligoCalc and BLAST). Results: Primer of PDE5 that is choosen is with reverse sequence 5-TGCATTGACCATGTCTCTCGTT-3, forward 5-CGCCGATCTGGGCTGAACTA-3 able to amplify template DNA at temperature 67.2°C, 65.8°C, 63.7°C, however, the DNA band fragment looks not very clear, while it is more clearly seen at Tm temperature 61.2°C, 59.1°C, 57.8°C and 57°C. Conclusion: PDE5 primers can be amplified well at temperature 61.2°C, 59.1°C, 57.8°C and 57°C. PDE5 primer succeeded in amplifying DNA with a product length of 402 bp.展开更多
Coral trout Plectropomus leopardus is a high-economical value of marine fish commodity.So,the larviculture for this species should be performed in hatchery scale for their sustainability.One of the important thing in ...Coral trout Plectropomus leopardus is a high-economical value of marine fish commodity.So,the larviculture for this species should be performed in hatchery scale for their sustainability.One of the important thing in larval stage is hormonal mechanisms.Thyroid hormone is known plays an important role in the metamorphosis of marine fish larvae.The source of that hormone is assumed derived from zooplankton as their live feeds.The live feeds commonly used for marine fish larvae in hatchery are zooplankton copepods and rotifers.The purpose of this research to determine the influence of feeding copepods and rotifers on thyroid hormone profile activity,consisted of triiodothyronine(T3)and thyroxine(T4),related to the metamorphoses of coral trout larvae.The research was conducted using three feeding schemes:copepods(A),copepods-rotifers(B),and rotifers(C).It was done for 50 Days After Hatching(DAH).The results showed that larvae from treatment(A)and(B)achieved the highest level of T3 and T4 earlier at 30 DAH,which indicated that both of those larvae have entered the metamorphosis stage earlier.On the other hand,larvae from treatment(C)achieved it and entered the metamorphosis stage later at 40 DAH.In addition,iodine levels in the copepods were significantly higher than those of in rotifers(P<0.05).Based on this research,live feeds copepods and rotifers have effect on thyroid hormone activity.Copepods have better potential in accelerating the metamorphosis achievement of coral trout larvae with the fact that they have higher iodine content.展开更多
文摘Background and Objective: Syzygium aromaticum is an immunomodulator with the main active compound eugenol which can stimulate the function of lymphocyte proliferation and macrophage production. Lymphocytes play an important role for body protection against infection. This study aims to analyze the effect of S. aromaticum extract to increase proliferation of lymphocytes, lymphoblasts and macrophage production in Balb/c mice infected with Salmonella typhimurium. Methodology: Mice strain Balb/c were divided into 4 groups: treatment group infected with S. typhimurium and control group (without treatment) to tested the activity of peritoneal macrophages. The treatment group was administrated by S. aromaticum leaf extract (15 mg, 75 mg, 150 mg/kgbw) for 12 days. Results: S. aromaticum leaf extract increased the proliferation activity (p Conclusion: Administration of S. aromaticum leaf extract increased the proliferation activity of lymphocytes, lymphoblasts and ROI secretion of macrophagesin Balb/c mice infected with S. typhimurium.
文摘Backround: Erectile dysfunction (ED) is the inability of male reproductive organs within sexual intercourse caused by neurogenic and hormonal disorders. There are some causes of ED such as hypertension, stress, neurological disorders, stroke, diabetes, atherosclerosis, lifestyle, alcohol, smoking, and age-related hormonal decline can cause infertility. The natural treatment of sexual dysfunction through aphrodisiac activity of the plant is to increase sexual hormones, spermatogenesis activity and through PDE5 inhibitors such as sildenafil, verdenafil, and tadalafil which can inhibit the hydrolysis of second messenger cGMP of penis smooth muscle cells. Primer Design for amplification of several PDE5 gene nucleotide sequences obtained from NCBI gen banks and tested directly through explosions at NCBI and also using MEGA 6, primer 3 plus, and fast PCR software. The natural treatment of sexual dysfunction through aphrodisiac activity from plants to increase sexual hormone, spermatogenesis activity and inhibitor PDE5 such as sildenafil, vardenafil, and tadalafil can inhibit hydrolysis second messenger of cells cGMP smooth penis muscles. Method: Primer design stages for several sequences are data supply, multiple sequence alignment, sequence trimming, primer design (fastPCR input), in silico PCR analysis, and primer evaluation (Primer Test, OligoCalc and BLAST). Results: Primer of PDE5 that is choosen is with reverse sequence 5-TGCATTGACCATGTCTCTCGTT-3, forward 5-CGCCGATCTGGGCTGAACTA-3 able to amplify template DNA at temperature 67.2°C, 65.8°C, 63.7°C, however, the DNA band fragment looks not very clear, while it is more clearly seen at Tm temperature 61.2°C, 59.1°C, 57.8°C and 57°C. Conclusion: PDE5 primers can be amplified well at temperature 61.2°C, 59.1°C, 57.8°C and 57°C. PDE5 primer succeeded in amplifying DNA with a product length of 402 bp.
文摘Coral trout Plectropomus leopardus is a high-economical value of marine fish commodity.So,the larviculture for this species should be performed in hatchery scale for their sustainability.One of the important thing in larval stage is hormonal mechanisms.Thyroid hormone is known plays an important role in the metamorphosis of marine fish larvae.The source of that hormone is assumed derived from zooplankton as their live feeds.The live feeds commonly used for marine fish larvae in hatchery are zooplankton copepods and rotifers.The purpose of this research to determine the influence of feeding copepods and rotifers on thyroid hormone profile activity,consisted of triiodothyronine(T3)and thyroxine(T4),related to the metamorphoses of coral trout larvae.The research was conducted using three feeding schemes:copepods(A),copepods-rotifers(B),and rotifers(C).It was done for 50 Days After Hatching(DAH).The results showed that larvae from treatment(A)and(B)achieved the highest level of T3 and T4 earlier at 30 DAH,which indicated that both of those larvae have entered the metamorphosis stage earlier.On the other hand,larvae from treatment(C)achieved it and entered the metamorphosis stage later at 40 DAH.In addition,iodine levels in the copepods were significantly higher than those of in rotifers(P<0.05).Based on this research,live feeds copepods and rotifers have effect on thyroid hormone activity.Copepods have better potential in accelerating the metamorphosis achievement of coral trout larvae with the fact that they have higher iodine content.
