Winged bean (Psophocarpus tetragonolobus (L.) DC.) is a potential legume crop of the tropics with high protein and oil content in the seeds. Analysis of the mutual genotypic relationships among twenty four genotypes o...Winged bean (Psophocarpus tetragonolobus (L.) DC.) is a potential legume crop of the tropics with high protein and oil content in the seeds. Analysis of the mutual genotypic relationships among twenty four genotypes of P. tetragonolobus through Mantel test found a significant correlation (r = 0.839) between similarity matrices of the results obtained from the use of the RAPD and ISSR molecular markers. The UPGMA tree based on Jaccard’s similarity coefficient generated from their cumulative data showed two distinct clusters and seven sub-clusters among these accessions. Quantification of total polyphenols, flavonoids and tannin revealed the highest percentage of occurrence of kaempferol (1.07-790.5 μg/g) and the lowest percentage of gallic acid (0.09-3.49 μg/g) in the seeds. Phytochemical analysis of the winged bean genotypes revealed that, some of the exotic lines are distinct. Analysis of photosynthesis rate, photosynthetic yield and stomatal conductance data also showed two clusters and was in congruence with the phytochemical affinities of the genotypes. The overall high level of polymorphism and varied range of genetic distances across the genotypes revealed a wide range of genetic base of P. tetragonolobus. The present investigation therefore, has provided significant insights for further improvement of winged bean germplasm for its qualitative and quantitative traits.展开更多
Degradable waste generated from food processing industries has immense potential for producing valuable products.Apples constitute among the most widely consumed fruits in the food processing industry,but their peels ...Degradable waste generated from food processing industries has immense potential for producing valuable products.Apples constitute among the most widely consumed fruits in the food processing industry,but their peels remain unutilized.We explored the possibility of using apple peel as feedstock for production of alpha-amylase-a commercially important enzyme with role in food industry and bio-ethanol production that is critical to support nutrition and energy security.The utilization of locally sourced apple peel as feedstock,optimization of the conditions for the production of alpha-amylase through solid-state fermentation and comparison with potato peel-another widely available feedstock option is done.The Bacillus subtilis BS1934 strain was identified as a promising strain that could produce alpha-amylase efficiently at 500C.The obtained values i.e.,17468 and 5229 U/L of alpha-amylase from apple and potato peel,respectively through solid state fermentation in eight days were considered as optimum.The enzyme titres were 3-fold higher with apple peel as compared to potato peel in the medium,indicating superior quality of apple peel feedstock as compared to potato for amylase production.The production of amylase using agro-industrial residues,as apple peel may emerge as a cost-effective alternative to amylase from microbial sources for industrial purpose.展开更多
文摘Winged bean (Psophocarpus tetragonolobus (L.) DC.) is a potential legume crop of the tropics with high protein and oil content in the seeds. Analysis of the mutual genotypic relationships among twenty four genotypes of P. tetragonolobus through Mantel test found a significant correlation (r = 0.839) between similarity matrices of the results obtained from the use of the RAPD and ISSR molecular markers. The UPGMA tree based on Jaccard’s similarity coefficient generated from their cumulative data showed two distinct clusters and seven sub-clusters among these accessions. Quantification of total polyphenols, flavonoids and tannin revealed the highest percentage of occurrence of kaempferol (1.07-790.5 μg/g) and the lowest percentage of gallic acid (0.09-3.49 μg/g) in the seeds. Phytochemical analysis of the winged bean genotypes revealed that, some of the exotic lines are distinct. Analysis of photosynthesis rate, photosynthetic yield and stomatal conductance data also showed two clusters and was in congruence with the phytochemical affinities of the genotypes. The overall high level of polymorphism and varied range of genetic distances across the genotypes revealed a wide range of genetic base of P. tetragonolobus. The present investigation therefore, has provided significant insights for further improvement of winged bean germplasm for its qualitative and quantitative traits.
基金This research did not receive any specific grant from funding agencies in the public,commercial,or not-for-profit sectors.
文摘Degradable waste generated from food processing industries has immense potential for producing valuable products.Apples constitute among the most widely consumed fruits in the food processing industry,but their peels remain unutilized.We explored the possibility of using apple peel as feedstock for production of alpha-amylase-a commercially important enzyme with role in food industry and bio-ethanol production that is critical to support nutrition and energy security.The utilization of locally sourced apple peel as feedstock,optimization of the conditions for the production of alpha-amylase through solid-state fermentation and comparison with potato peel-another widely available feedstock option is done.The Bacillus subtilis BS1934 strain was identified as a promising strain that could produce alpha-amylase efficiently at 500C.The obtained values i.e.,17468 and 5229 U/L of alpha-amylase from apple and potato peel,respectively through solid state fermentation in eight days were considered as optimum.The enzyme titres were 3-fold higher with apple peel as compared to potato peel in the medium,indicating superior quality of apple peel feedstock as compared to potato for amylase production.The production of amylase using agro-industrial residues,as apple peel may emerge as a cost-effective alternative to amylase from microbial sources for industrial purpose.
