Objective:To focus on the growth rate and feed utilization of fish by using trash fish feeds supplement with marine seaweeds.Methods:Selected seaweed was extracted using hot-water and its extract was mixed with trash ...Objective:To focus on the growth rate and feed utilization of fish by using trash fish feeds supplement with marine seaweeds.Methods:Selected seaweed was extracted using hot-water and its extract was mixed with trash fish feed at different concentrations(0.5%,1%and 2%for 1-30 days)and the non-specific immune response in fish was studied and challenged with Vibrio alginolyticus at 1×106 CFU/fish.The hot-water extract of seaweeds was analysed by gas chromatography-mass spectrometry.Results:The average body weight(5.320±0.018),percent weight gain(227.66±0.28),specific growth rate(2.080±0.015),hepatosomatic index(1.197±0.00)and viscerosomatic index(4.421±0.150)were significantly increased in the fish feed with seaweed containing 5%of Sargassum wightii(S.wightii)when compared with other seaweeds and control diet.Hot-water extract of S.wightii(1%)was significantly enhanced the immune response in fish when compared with other diets(0.5%and 2%).S.wightii showed good immunostimulation properties.Gas chromatography-mass spectrometry result showed that the hot-water extract of S.wightii seaweed contained fatty acids.Conclusions:Trash fish feed will reduce the production cost and also provide evidence that aqueous leaf extract of S.wightii(1%)was added to a formulated fish diet which could activate the non-specific immune response and disease resistance against Vibrio alginolyticus in Mugil cephalus.展开更多
Objective:To isolate bioactive molecules from marine seaweeds and check the antimicrobial activity against the fish pathogenic bacteria.Methods:Fresh marine seaweeds Gracilaria edulis,Kappaphycus spicifera,Sargassum w...Objective:To isolate bioactive molecules from marine seaweeds and check the antimicrobial activity against the fish pathogenic bacteria.Methods:Fresh marine seaweeds Gracilaria edulis,Kappaphycus spicifera,Sargassum wightii(S.wightii)were collected.Each seaweed was extracted with different solvents.In the study,test pathogens were collected from microbial type culture collection.Antibacterial activity was carried out by using disc diffusion method and minimum inhibition concentration(MIC)was calculated.Best seaweed was analysed by fourier transform infrared spectroscopy.The cured extract was separated by thin layer chromatography(TLC).Fraction was collected from TLC to check the antimicrobial activity.Best fraction was analysed by gas chromatography mass spectrometer(GCMS).Results:Based on the disc diffusion method,S.wightii showed a better antimicrobial activity than other seaweed extracts.Based on the MIC,methanol extract of S.wightii showed lower MIC than other solvents.S.wightii were separated by TLC.In this TLC,plate showed a two fraction.These two fractions were separated in preparative TLC and checked for their antimicrobial activity.Fraction 2 showed best MIC value against the tested pathogen.Fraction 2 was analysed by GCMS.Based on the GCMS,fraction 2 contains n-hexadecanoic acid(59.44%).Conclusions:From this present study,it can be concluded that S.wightii was potential sources of bioactive compounds.展开更多
Objective:Fresh marine seaweeds Gracilaria edulis,Gracillaria verrcosa,Acanthospora spicifera,Ulva facita,Ulva lacta(U.lacta),Kappaphycus spicifera,Sargassum ilicifolium,Sargassum wightii(S.wightii),Padina tetramatica...Objective:Fresh marine seaweeds Gracilaria edulis,Gracillaria verrcosa,Acanthospora spicifera,Ulva facita,Ulva lacta(U.lacta),Kappaphycus spicifera,Sargassum ilicifolium,Sargassum wightii(S.wightii),Padina tetramatica and Padina gymonospora were collected from Mandapam(Rameshwaram,Tamil Nadu)of South East coast of India and were screened for antibacterial activity.Methods:All the collected seaweeds were extracted by using five different solvent(methanol,isopropanol,acetone,chloroform,diethyl ether)to study their extracts against fish pathogenic bacteria V.alginolyticus(VA09)purchased from MTCC.And minimum inhibition carried out by using Resazurin micro-titre assay.Crude extract of S.wightii analysied by FTIR.Results:The methanolic extract of S.wightii produced a maximum zone of inhibition(1.95±0.11)cm,isopropanol extract maximum inhibition was produced by S.wightii(1.93±0.78)cm,Acetone extract of Gracilaria verrcosa showed maximum zone of inhibition(1.36±0.05)cm,chloroform extract of S.wightii produced a maximum zone(1.56±0.25)cm and diethyl ether extract of S.wightii produced maximum zone of inhibition(1.86±0.11)cm.Based on the antibacterial activity S.wightii,U.lacta and Padina tetramatica showed best antibacterial activity against Vibrio harveyi.In this three seaweeds were taken for MIC study.The S.wightii methanolic extract,U.lacta diethyl ether extract and Padina tetramatica methanolic extract showed a higher MIC values,and despectively were 25 mg/mL,50 mg/mL and 50 mg/mL.FTIR result showed that mostly phenolic compounds were present in the S.wightii.Conclusions:Based on the FTIR result S.wightii have high amount of phenolic compound.Phenolic compound have the good antimicrobial activity.The results clearly show that seaweedS.wightii is an interesting source for biologically active compounds that may be applied for prophylaxis and therapy of bacterial fish diseases and it should be used for immunostimulation activity in aquaculture.展开更多
基金Supported by grants from the Science and Engineering Research Board(SERB-F.No.SR/FT/LS-142/2009),Department of Science and Technology,Government of India,New Delhi.
文摘Objective:To focus on the growth rate and feed utilization of fish by using trash fish feeds supplement with marine seaweeds.Methods:Selected seaweed was extracted using hot-water and its extract was mixed with trash fish feed at different concentrations(0.5%,1%and 2%for 1-30 days)and the non-specific immune response in fish was studied and challenged with Vibrio alginolyticus at 1×106 CFU/fish.The hot-water extract of seaweeds was analysed by gas chromatography-mass spectrometry.Results:The average body weight(5.320±0.018),percent weight gain(227.66±0.28),specific growth rate(2.080±0.015),hepatosomatic index(1.197±0.00)and viscerosomatic index(4.421±0.150)were significantly increased in the fish feed with seaweed containing 5%of Sargassum wightii(S.wightii)when compared with other seaweeds and control diet.Hot-water extract of S.wightii(1%)was significantly enhanced the immune response in fish when compared with other diets(0.5%and 2%).S.wightii showed good immunostimulation properties.Gas chromatography-mass spectrometry result showed that the hot-water extract of S.wightii seaweed contained fatty acids.Conclusions:Trash fish feed will reduce the production cost and also provide evidence that aqueous leaf extract of S.wightii(1%)was added to a formulated fish diet which could activate the non-specific immune response and disease resistance against Vibrio alginolyticus in Mugil cephalus.
基金Supported by the Science and Engineering Research Board(SERB-F.No.SR/FT/LS-142/2009)Department of Science and Technology(DST),Government of India,New Delhi.
文摘Objective:To isolate bioactive molecules from marine seaweeds and check the antimicrobial activity against the fish pathogenic bacteria.Methods:Fresh marine seaweeds Gracilaria edulis,Kappaphycus spicifera,Sargassum wightii(S.wightii)were collected.Each seaweed was extracted with different solvents.In the study,test pathogens were collected from microbial type culture collection.Antibacterial activity was carried out by using disc diffusion method and minimum inhibition concentration(MIC)was calculated.Best seaweed was analysed by fourier transform infrared spectroscopy.The cured extract was separated by thin layer chromatography(TLC).Fraction was collected from TLC to check the antimicrobial activity.Best fraction was analysed by gas chromatography mass spectrometer(GCMS).Results:Based on the disc diffusion method,S.wightii showed a better antimicrobial activity than other seaweed extracts.Based on the MIC,methanol extract of S.wightii showed lower MIC than other solvents.S.wightii were separated by TLC.In this TLC,plate showed a two fraction.These two fractions were separated in preparative TLC and checked for their antimicrobial activity.Fraction 2 showed best MIC value against the tested pathogen.Fraction 2 was analysed by GCMS.Based on the GCMS,fraction 2 contains n-hexadecanoic acid(59.44%).Conclusions:From this present study,it can be concluded that S.wightii was potential sources of bioactive compounds.
基金Supported by Science and Engineering Research Board(SERB-F.No.SR/FT/LS-142/2009)Department of Science and Technology(DST),Government of India,New Delhi.
文摘Objective:Fresh marine seaweeds Gracilaria edulis,Gracillaria verrcosa,Acanthospora spicifera,Ulva facita,Ulva lacta(U.lacta),Kappaphycus spicifera,Sargassum ilicifolium,Sargassum wightii(S.wightii),Padina tetramatica and Padina gymonospora were collected from Mandapam(Rameshwaram,Tamil Nadu)of South East coast of India and were screened for antibacterial activity.Methods:All the collected seaweeds were extracted by using five different solvent(methanol,isopropanol,acetone,chloroform,diethyl ether)to study their extracts against fish pathogenic bacteria V.alginolyticus(VA09)purchased from MTCC.And minimum inhibition carried out by using Resazurin micro-titre assay.Crude extract of S.wightii analysied by FTIR.Results:The methanolic extract of S.wightii produced a maximum zone of inhibition(1.95±0.11)cm,isopropanol extract maximum inhibition was produced by S.wightii(1.93±0.78)cm,Acetone extract of Gracilaria verrcosa showed maximum zone of inhibition(1.36±0.05)cm,chloroform extract of S.wightii produced a maximum zone(1.56±0.25)cm and diethyl ether extract of S.wightii produced maximum zone of inhibition(1.86±0.11)cm.Based on the antibacterial activity S.wightii,U.lacta and Padina tetramatica showed best antibacterial activity against Vibrio harveyi.In this three seaweeds were taken for MIC study.The S.wightii methanolic extract,U.lacta diethyl ether extract and Padina tetramatica methanolic extract showed a higher MIC values,and despectively were 25 mg/mL,50 mg/mL and 50 mg/mL.FTIR result showed that mostly phenolic compounds were present in the S.wightii.Conclusions:Based on the FTIR result S.wightii have high amount of phenolic compound.Phenolic compound have the good antimicrobial activity.The results clearly show that seaweedS.wightii is an interesting source for biologically active compounds that may be applied for prophylaxis and therapy of bacterial fish diseases and it should be used for immunostimulation activity in aquaculture.
