Cyclic variability is a factor adversely affecting engine performance. In this paper a cyclic moving average regulation approach to cylinder pressure at top dead center （TDC） is proposed, where the ignition time is ...Cyclic variability is a factor adversely affecting engine performance. In this paper a cyclic moving average regulation approach to cylinder pressure at top dead center （TDC） is proposed, where the ignition time is adopted as the control input. The dynamics from ignition time to the moving average index is described by ARMA model. With this model, a one-step ahead prediction-based minimum variance controller （MVC） is developed for regulation. The performance of the proposed controller is illustrated by experiments with a commercial car engine and experimental results show that the controller has a reliable effect on index regulation when the engine works under different fuel injection strategies, load changing and throttle opening disturbance.展开更多
Autoimmune hepatitis(AIH)is a chronic inflammatory liver disease that threatens human health worldwide.The aim of this study was to detect the protective effect of a fermented Lentinus edodes extract containingα-gluc...Autoimmune hepatitis(AIH)is a chronic inflammatory liver disease that threatens human health worldwide.The aim of this study was to detect the protective effect of a fermented Lentinus edodes extract containingα-glucan(FLA),in a concanavalin A(Con A)-induced AIH mouse model and to determine the underlying liver-protective mechanism.The results showed that compared with the model group,the level of proinflammatory cytokines in serum of FLA pretreated mice was significantly decreased,and the degree of inflammatory cell infiltration in liver,thymus and spleen was significantly reduced.Quantitative polymerase chain reaction,immunohistochemistry,and Western blotting showed that FLA pre-treatment inhibited the Con A-induced apoptosis of hepatocytes by down-regulating the expression of BAX and up-regulating the expression of BCL-2.Further research found that FLA may improve liver injury in mice by activating NRF2 signaling pathway and inhibiting TRAF6/NF-κB signaling pathway.Thus,FLA may improve liver injury in mice by shifting gut microbial composition to reduce the release of inflammatory cytokines in the serum and prevent the necrosis of hepatocytes.Up-regulation of NRF2 signaling pathway,down-regulation of TRAF6/NF-κB signaling pathway,and an increase in the relative abundance of Lactobacillus_johnsonii and Ligilactobacillus_murinus play a protective role in liver.展开更多
MicroRNA(miRNA)is promising candidate for non-invasive diagnostic biomarker.Conventional CRISPR/Cas12a-based miRNA detection systems are constrained by reliance on reverse transcription,nucleic acid preamplification a...MicroRNA(miRNA)is promising candidate for non-invasive diagnostic biomarker.Conventional CRISPR/Cas12a-based miRNA detection systems are constrained by reliance on reverse transcription,nucleic acid preamplification and costly fluorescently labeled reporters which introduce chemical modification complexity and background noise.To address these limitations,we herein developed a multifunctional switch that inte-grated target recognition,CRISPR/Cas12a system activation,intrinsic fluorescence signaling,and autonomous signal amplification within a single molecular architecture.As a proof of concept,this switch enabled a label-free CRISPR/Cas12a biosensing for miR-21 detection with a detection limit of 4.8 nM and robust performance in accuracy,precision,and selectivity.This proposed label-free CRISPR/Cas12a platform could be applied for real sample and is a promising candidate for point-of-care miRNA detection.展开更多
文摘Cyclic variability is a factor adversely affecting engine performance. In this paper a cyclic moving average regulation approach to cylinder pressure at top dead center （TDC） is proposed, where the ignition time is adopted as the control input. The dynamics from ignition time to the moving average index is described by ARMA model. With this model, a one-step ahead prediction-based minimum variance controller （MVC） is developed for regulation. The performance of the proposed controller is illustrated by experiments with a commercial car engine and experimental results show that the controller has a reliable effect on index regulation when the engine works under different fuel injection strategies, load changing and throttle opening disturbance.
基金supported by the Shanghai Lithy One-Health Group Technology Co.,Ltd.,Project(114-KH210230A)。
文摘Autoimmune hepatitis(AIH)is a chronic inflammatory liver disease that threatens human health worldwide.The aim of this study was to detect the protective effect of a fermented Lentinus edodes extract containingα-glucan(FLA),in a concanavalin A(Con A)-induced AIH mouse model and to determine the underlying liver-protective mechanism.The results showed that compared with the model group,the level of proinflammatory cytokines in serum of FLA pretreated mice was significantly decreased,and the degree of inflammatory cell infiltration in liver,thymus and spleen was significantly reduced.Quantitative polymerase chain reaction,immunohistochemistry,and Western blotting showed that FLA pre-treatment inhibited the Con A-induced apoptosis of hepatocytes by down-regulating the expression of BAX and up-regulating the expression of BCL-2.Further research found that FLA may improve liver injury in mice by activating NRF2 signaling pathway and inhibiting TRAF6/NF-κB signaling pathway.Thus,FLA may improve liver injury in mice by shifting gut microbial composition to reduce the release of inflammatory cytokines in the serum and prevent the necrosis of hepatocytes.Up-regulation of NRF2 signaling pathway,down-regulation of TRAF6/NF-κB signaling pathway,and an increase in the relative abundance of Lactobacillus_johnsonii and Ligilactobacillus_murinus play a protective role in liver.
基金supported by Natural Science Foundation of Henan Province(Grant No.242300421266)China Postdoctoral Science Foundation(Grant No.2020M672298)+1 种基金Cultivation Program for Basic Research of Natural Sciences in Zhengzhou University(Grant No.JC22846048)the Medical Science and Technology Research Program of Henan Province(Grant No.SBGJ202101018).
文摘MicroRNA(miRNA)is promising candidate for non-invasive diagnostic biomarker.Conventional CRISPR/Cas12a-based miRNA detection systems are constrained by reliance on reverse transcription,nucleic acid preamplification and costly fluorescently labeled reporters which introduce chemical modification complexity and background noise.To address these limitations,we herein developed a multifunctional switch that inte-grated target recognition,CRISPR/Cas12a system activation,intrinsic fluorescence signaling,and autonomous signal amplification within a single molecular architecture.As a proof of concept,this switch enabled a label-free CRISPR/Cas12a biosensing for miR-21 detection with a detection limit of 4.8 nM and robust performance in accuracy,precision,and selectivity.This proposed label-free CRISPR/Cas12a platform could be applied for real sample and is a promising candidate for point-of-care miRNA detection.
