Micro-emulsion usually consists of water, oil, surfactants and co-surfactants, and each component has an effect on the phase behavior and solubilization of the micro-emulsion. When the surfactant in the micro-emulsion...Micro-emulsion usually consists of water, oil, surfactants and co-surfactants, and each component has an effect on the phase behavior and solubilization of the micro-emulsion. When the surfactant in the micro-emulsion system is quaternary ammonium cationic Gemini surfactant, the surfactant mainly combines with the anions in the salt. With the increase of salt concentration, the phase transformation of Winsor I → Winsor III → Winsor II occurred, but the optimum salinity and salt width are different because of the type of salt. The effects of 5 different kinds of monovalent anions, including C_6H_5SO_3^-, I-, Br-, NO_3^- and Cl-, on the phase behavior and solubilization of quaternary ammonium cationic Gemini micro-emulsion are researched by Winsor phase diagram. It is found that the effects of organic anions C_6H_5SO_3-and I-on the phase behavior and solubilization of quaternary ammonium cationic Gemini micro-emulsion are most significant, and the effects of Br-, NO_3^- and Cl-are less significant. Meanwhile, when the optimum solubilization is achieved, the amount of sodium benzoate is the least, indicating that the organic anion has stronger self-organization behavior with quaternary ammonium cationic Gemini surfactants.展开更多
Developing biosensors to monitor and regulate intracellular biosynthesis pathways can significantly enhance natural product yields in microbial cell factories.This study created a novel biosensor in Saccharomyces cere...Developing biosensors to monitor and regulate intracellular biosynthesis pathways can significantly enhance natural product yields in microbial cell factories.This study created a novel biosensor in Saccharomyces cerevisiae to respond to p-coumaric acid,a critical precursor in the biosynthesis of polyphenols and flavonoids.This biosensor was constructed by expressing the BsPadR repressor from Bacillus subtilis and engineering hybrid promoters.Notably,the PBS1-CCW12 hybrid promoter exhibited tight regulation by BsPadR and enhanced activity in response to p-coumaric acid.However,excessive BsPadR expression negatively impacted yeast growth,which was mitigated by using weaker promoters,PBST1 and PERG9.Furthermore,the impact of nuclear localization signal(SV40-NLS)positioning on BsPadR functionality was explored,revealing that fusion of an SV40-NLS at the C-terminus of BsPadR enhanced the biosensor’s performance.To validate its utility,we applied this system to dynamically regulate CrtE(geranylgeranyl pyrophosphate synthase),a key enzyme in lycopene biosynthesis.By coupling p-coumaric acid production with lycopene biosynthesis,we enabled high-throughput colorimetric screening for enzyme evolution and strain selection.This novel biosensor serves as a valuable tool for future studies aimed at optimizing the production of p-coumaric acid and its derivatives in S.cerevisiae,thereby advancing the efficiency of biosynthetic processes in microbial cell factories.展开更多
Background:To compare the efficacy and safety of preoperative intravitreal injections of ranibizumab and conbercept in Chinese proliferative diabetic retinopathy(PDR)patients.Methods:This prospective randomized contro...Background:To compare the efficacy and safety of preoperative intravitreal injections of ranibizumab and conbercept in Chinese proliferative diabetic retinopathy(PDR)patients.Methods:This prospective randomized controlled trial enrolled 90 eyes of 80 patients with PDR.Forty-four eyes of 40 patients that received intravitreal ranibizumab(IVR)injections(0.5 mg/0.05 mL)before vitreous surgeries were assigned to the IVR group.Forty-six eyes of 40 patients that received intravitreal conbercept(IVC)injections(0.5 mg/0.05 mL)before vitreous surgeries were assigned to the IVC group.Intraoperative and postoperative indices were assessed for further comparison between the two groups.Results:There were no statistically significant differences in all surgery indices,including intraoperative indices(surgery time,P=0.225;intraoperative bleeding,P=0.808;endodiathermy use,P=0.693;incidence of iatrogenic retinal breaks,P=0.740;relaxing retinotomy,P=0.682;retinal reattachment,P=0.682 and silicone oil tamponade,P=0.814)and postoperative indices(postoperative vitreous hemorrhage(VH),P=0.808;neovascular glaucoma(NVG),P=0.964;recurrent retinal detachment,P=0.531;postoperative fibrovascular proliferation progression,P=0.682 and reoperation,P=0.955)between the two groups.There were no statistically significant differences in best-corrected visual acuity(BCVA)at each follow-up visit(P=0.939,0.669,0.741 and 0.717,respectively)or in central retinal thickness(CRT)(P=0.976,0.699,0.551 and 0.686,respectively).As for safety profile,both groups had no ocular or system adverse events during the observation period.Conclusions:IVR and IVC as a pretreatment of vitrectomy had similar efficacy and safety profile for Chinese PDR patients.展开更多
基金Supported by China Petroleum Science and Technology Innovation Fund(2017D-5007-0601)State Key Laboratory of Heavy Oil Processing and China University of Petroleum(East China)2018 Graduate Engineering Innovation Project Found(SLKZZ-2017002)
文摘Micro-emulsion usually consists of water, oil, surfactants and co-surfactants, and each component has an effect on the phase behavior and solubilization of the micro-emulsion. When the surfactant in the micro-emulsion system is quaternary ammonium cationic Gemini surfactant, the surfactant mainly combines with the anions in the salt. With the increase of salt concentration, the phase transformation of Winsor I → Winsor III → Winsor II occurred, but the optimum salinity and salt width are different because of the type of salt. The effects of 5 different kinds of monovalent anions, including C_6H_5SO_3^-, I-, Br-, NO_3^- and Cl-, on the phase behavior and solubilization of quaternary ammonium cationic Gemini micro-emulsion are researched by Winsor phase diagram. It is found that the effects of organic anions C_6H_5SO_3-and I-on the phase behavior and solubilization of quaternary ammonium cationic Gemini micro-emulsion are most significant, and the effects of Br-, NO_3^- and Cl-are less significant. Meanwhile, when the optimum solubilization is achieved, the amount of sodium benzoate is the least, indicating that the organic anion has stronger self-organization behavior with quaternary ammonium cationic Gemini surfactants.
基金supported by National Natural Science Foundation of China(Grant No.32371480)High-level Talent Support Program of Yangzhou University,Postgraduate Research&Practice Innovation Program of Jiangsu Province(Grant No.SJCX25_2391).
文摘Developing biosensors to monitor and regulate intracellular biosynthesis pathways can significantly enhance natural product yields in microbial cell factories.This study created a novel biosensor in Saccharomyces cerevisiae to respond to p-coumaric acid,a critical precursor in the biosynthesis of polyphenols and flavonoids.This biosensor was constructed by expressing the BsPadR repressor from Bacillus subtilis and engineering hybrid promoters.Notably,the PBS1-CCW12 hybrid promoter exhibited tight regulation by BsPadR and enhanced activity in response to p-coumaric acid.However,excessive BsPadR expression negatively impacted yeast growth,which was mitigated by using weaker promoters,PBST1 and PERG9.Furthermore,the impact of nuclear localization signal(SV40-NLS)positioning on BsPadR functionality was explored,revealing that fusion of an SV40-NLS at the C-terminus of BsPadR enhanced the biosensor’s performance.To validate its utility,we applied this system to dynamically regulate CrtE(geranylgeranyl pyrophosphate synthase),a key enzyme in lycopene biosynthesis.By coupling p-coumaric acid production with lycopene biosynthesis,we enabled high-throughput colorimetric screening for enzyme evolution and strain selection.This novel biosensor serves as a valuable tool for future studies aimed at optimizing the production of p-coumaric acid and its derivatives in S.cerevisiae,thereby advancing the efficiency of biosynthetic processes in microbial cell factories.
基金funded by Capital Clinical Diagnosis and Treatment Technology Research and Demonstration Application Project of China(Grant No.Z191100006619029)National Key R&D Program of China(Grant No.2020YFC2008200).
文摘Background:To compare the efficacy and safety of preoperative intravitreal injections of ranibizumab and conbercept in Chinese proliferative diabetic retinopathy(PDR)patients.Methods:This prospective randomized controlled trial enrolled 90 eyes of 80 patients with PDR.Forty-four eyes of 40 patients that received intravitreal ranibizumab(IVR)injections(0.5 mg/0.05 mL)before vitreous surgeries were assigned to the IVR group.Forty-six eyes of 40 patients that received intravitreal conbercept(IVC)injections(0.5 mg/0.05 mL)before vitreous surgeries were assigned to the IVC group.Intraoperative and postoperative indices were assessed for further comparison between the two groups.Results:There were no statistically significant differences in all surgery indices,including intraoperative indices(surgery time,P=0.225;intraoperative bleeding,P=0.808;endodiathermy use,P=0.693;incidence of iatrogenic retinal breaks,P=0.740;relaxing retinotomy,P=0.682;retinal reattachment,P=0.682 and silicone oil tamponade,P=0.814)and postoperative indices(postoperative vitreous hemorrhage(VH),P=0.808;neovascular glaucoma(NVG),P=0.964;recurrent retinal detachment,P=0.531;postoperative fibrovascular proliferation progression,P=0.682 and reoperation,P=0.955)between the two groups.There were no statistically significant differences in best-corrected visual acuity(BCVA)at each follow-up visit(P=0.939,0.669,0.741 and 0.717,respectively)or in central retinal thickness(CRT)(P=0.976,0.699,0.551 and 0.686,respectively).As for safety profile,both groups had no ocular or system adverse events during the observation period.Conclusions:IVR and IVC as a pretreatment of vitrectomy had similar efficacy and safety profile for Chinese PDR patients.
