Mucin-degrading bacteria play a critical role in maintaining gut homeostasis,while the interspecies interactions remain unclear.In this study,we analyzed metagenomic data from 9319 human gut samples across various hea...Mucin-degrading bacteria play a critical role in maintaining gut homeostasis,while the interspecies interactions remain unclear.In this study,we analyzed metagenomic data from 9319 human gut samples across various health conditions and identified two antagonistic mucin-degrading microbial networks,dominated by Akker-mansia muciniphila(A.muciniphila)and Ruminococcus gnavus(R.gnavus),respectively.The A.muciniphila-dominated network was significantly depleted in individuals with colitis,whereas the R.gnavus-dominated network was enriched in these patients.Then,a Transwell-based in vitro co-culture system revealed that Bac-teroides cellulosilyticus(B.cellulosilyticus)promoted A.muciniphila growth in a mucin-dependent manner(p<0.001),while Bacteroides fragilis(B.fragilis)selectively enhanced the proliferation of R.gnavus(p<0.01).We further validated the stability of interspecies relationships among mucin-degrading bacteria in SPF mice,and in a DSS-induced mouse model,administration of A.muciniphila alleviated inflammation,whereas R.gnavus exac-erbated colitis,potentially through divergent short-chain fatty acid(SCFAs)metabolic profiles.Collectively,these findings reveal a structured interaction network within the mucin-degrading microbiota and highlight its critical role in shaping intestinal immune responses,offering new avenues for microbiota-targeted strategies in the treatment of mucosal inflammatory diseases.展开更多
基金supported by the National Natural Science Foundation of China[No.32425044 and U23A20259]supported by the Fundamental Research Funds for the Central Universities JUSRP622013Collaborative innovation center of food safety and quality control in Jiangsu Province.
文摘Mucin-degrading bacteria play a critical role in maintaining gut homeostasis,while the interspecies interactions remain unclear.In this study,we analyzed metagenomic data from 9319 human gut samples across various health conditions and identified two antagonistic mucin-degrading microbial networks,dominated by Akker-mansia muciniphila(A.muciniphila)and Ruminococcus gnavus(R.gnavus),respectively.The A.muciniphila-dominated network was significantly depleted in individuals with colitis,whereas the R.gnavus-dominated network was enriched in these patients.Then,a Transwell-based in vitro co-culture system revealed that Bac-teroides cellulosilyticus(B.cellulosilyticus)promoted A.muciniphila growth in a mucin-dependent manner(p<0.001),while Bacteroides fragilis(B.fragilis)selectively enhanced the proliferation of R.gnavus(p<0.01).We further validated the stability of interspecies relationships among mucin-degrading bacteria in SPF mice,and in a DSS-induced mouse model,administration of A.muciniphila alleviated inflammation,whereas R.gnavus exac-erbated colitis,potentially through divergent short-chain fatty acid(SCFAs)metabolic profiles.Collectively,these findings reveal a structured interaction network within the mucin-degrading microbiota and highlight its critical role in shaping intestinal immune responses,offering new avenues for microbiota-targeted strategies in the treatment of mucosal inflammatory diseases.
