Homeostasis of platelet number in human and other mammals is well maintained for prevention of minor bleeding and for other im- munological functions, but the exact molecular mechanism responsible for immune thrombocy...Homeostasis of platelet number in human and other mammals is well maintained for prevention of minor bleeding and for other im- munological functions, but the exact molecular mechanism responsible for immune thrombocytopenic purpura （ITP） has not been fully understood. In an effort to identify genetic factors involved in initiation of platelet production in response to bleeding injury or platelet destruction, we have successfully generated an animal model of human ITP via intraperitoneal injection of anti-platelet antibody into the Balb/c mouse. Platelet counts were dropped dramatically in animals that received antibody injection within 4 h, maintained at the mini- mum level for a period of 44 h, started to rebound after 48 h, and reached to the maximum at 144 h （6 days）. Final homeostasis reached at approximately 408 h （17 days）, following a minor cycle of platelet number fluctuation. Using semi-quantitative RT-PCR, we assessed and compared mRNA level of CD41, c-myb, c-mpl, caspase-3, caspase-9, GATA-1, and Bcl-xl in bone marrow and spleen. Alteration of mRNA expression was correlated with the change of platelet level, and an inverse relationship was found for expression of the genes be- tween bone marrow and spleen. No transcription was detectable for any of the seven genes in bone marrow at the time when platelet number reached the maximum （144 h）. In contrast, mRNA transcripts of the seven genes were found to be at the highest level in spleen tissue. This is the first study of simultaneous detection of multiple platelet related genes in a highly reproducible ITP animal model. Our results provided the supportive evidence that expression of the above seven genes are more related to negative regulation of platelet number in spleen tissue, at least in the model animals.展开更多
Chinese Kun Ming(KM)mouse,an outbreed strain of laboratory animal,has been widely utilized in related pharmaceutical and genetic studies throughout China.However,the value of KM mice to the research community has been...Chinese Kun Ming(KM)mouse,an outbreed strain of laboratory animal,has been widely utilized in related pharmaceutical and genetic studies throughout China.However,the value of KM mice to the research community has been severely limited,partially due to the fact that well-characterized inbred strain of KM mice is not available.Several expected inbred strains from KM mice have been bred,but their genetic purity remains uncertain.In this study,four expected inbred strains of KM mice(A1,T2,N2,and N4)were chosen and their inbred degree were compared with two classical inbred mouse lines(BALB/c and C57BL/6)by analyzing the genotypes of about 30 microsatellite markers.In the four strains,A1 and N4 were homozygous at all genotyped loci,but N2 and T2 were only heterozygous at locus D15Mit16.These results indicate that the level of genetic purity/homozygousity of A1,N4,N2,and T2 inbred line is comparable to those of BALB/c and C57BL/6.This study provided the first and solid evidence for genetic purity of four expected inbred strains of KM mice.These 4 inbred mice strains should be well maintained for further characterization and utilization in genetic studies.展开更多
Within a honeybee population, due to polyandry, there are super-sister and half-sister relations, thus many sub-families exist. For the Chinese honeybee (Apis cerana cerana F.) a phylogenetic dendrogram has been const...Within a honeybee population, due to polyandry, there are super-sister and half-sister relations, thus many sub-families exist. For the Chinese honeybee (Apis cerana cerana F.) a phylogenetic dendrogram has been constructed in which 4 sub-families are clustered based on DNA fingerprint patterns. And it has been observed that each kind of workers is distributed to several different sub-families.展开更多
文摘Homeostasis of platelet number in human and other mammals is well maintained for prevention of minor bleeding and for other im- munological functions, but the exact molecular mechanism responsible for immune thrombocytopenic purpura （ITP） has not been fully understood. In an effort to identify genetic factors involved in initiation of platelet production in response to bleeding injury or platelet destruction, we have successfully generated an animal model of human ITP via intraperitoneal injection of anti-platelet antibody into the Balb/c mouse. Platelet counts were dropped dramatically in animals that received antibody injection within 4 h, maintained at the mini- mum level for a period of 44 h, started to rebound after 48 h, and reached to the maximum at 144 h （6 days）. Final homeostasis reached at approximately 408 h （17 days）, following a minor cycle of platelet number fluctuation. Using semi-quantitative RT-PCR, we assessed and compared mRNA level of CD41, c-myb, c-mpl, caspase-3, caspase-9, GATA-1, and Bcl-xl in bone marrow and spleen. Alteration of mRNA expression was correlated with the change of platelet level, and an inverse relationship was found for expression of the genes be- tween bone marrow and spleen. No transcription was detectable for any of the seven genes in bone marrow at the time when platelet number reached the maximum （144 h）. In contrast, mRNA transcripts of the seven genes were found to be at the highest level in spleen tissue. This is the first study of simultaneous detection of multiple platelet related genes in a highly reproducible ITP animal model. Our results provided the supportive evidence that expression of the above seven genes are more related to negative regulation of platelet number in spleen tissue, at least in the model animals.
基金This work was supported by the National Natural Science Foundation of China(No.03114121).
文摘Chinese Kun Ming(KM)mouse,an outbreed strain of laboratory animal,has been widely utilized in related pharmaceutical and genetic studies throughout China.However,the value of KM mice to the research community has been severely limited,partially due to the fact that well-characterized inbred strain of KM mice is not available.Several expected inbred strains from KM mice have been bred,but their genetic purity remains uncertain.In this study,four expected inbred strains of KM mice(A1,T2,N2,and N4)were chosen and their inbred degree were compared with two classical inbred mouse lines(BALB/c and C57BL/6)by analyzing the genotypes of about 30 microsatellite markers.In the four strains,A1 and N4 were homozygous at all genotyped loci,but N2 and T2 were only heterozygous at locus D15Mit16.These results indicate that the level of genetic purity/homozygousity of A1,N4,N2,and T2 inbred line is comparable to those of BALB/c and C57BL/6.This study provided the first and solid evidence for genetic purity of four expected inbred strains of KM mice.These 4 inbred mice strains should be well maintained for further characterization and utilization in genetic studies.
文摘Within a honeybee population, due to polyandry, there are super-sister and half-sister relations, thus many sub-families exist. For the Chinese honeybee (Apis cerana cerana F.) a phylogenetic dendrogram has been constructed in which 4 sub-families are clustered based on DNA fingerprint patterns. And it has been observed that each kind of workers is distributed to several different sub-families.
