目的:探讨DC的抗肿瘤免疫功能是否与其基因位点有关。方法:选择19株基因型明确的BXD纯系小鼠的树突状细胞(DC),MTT法和ELISA分析对DC吞噬肿瘤细胞的作用;肿瘤细胞诱导:DC产生IL-12的能力;经肿瘤细胞刺激后的DC对T细胞增殖和:IFN-γ分泌...目的:探讨DC的抗肿瘤免疫功能是否与其基因位点有关。方法:选择19株基因型明确的BXD纯系小鼠的树突状细胞(DC),MTT法和ELISA分析对DC吞噬肿瘤细胞的作用;肿瘤细胞诱导:DC产生IL-12的能力;经肿瘤细胞刺激后的DC对T细胞增殖和:IFN-γ分泌的影响进行测定。用流式细胞仪检测:DC表面抗原标志CD80和CD54。有关数据用MapManagerQTX and WebQTL软件对DC抗肿瘤免疫的基因位点进行分析。结果:DC刺激T细胞增殖的作用很大程度取决于BXD各系的遗传差异。DC吞噬肿瘤细胞及诱导IL-12产生的能力都与DC刺激T细胞的增殖相一致(P<0.01)。基因位点定量分析(QTL)分析在第6和第13号染色体上有2个位点可能与DC的肿瘤抗原提呈加工功能和T细胞毒作用有关。结论:不同系的BXD小鼠,其DC的抗原提呈、加工能力不同。DC对肿瘤细胞生长的抑制与IL-12的诱导产生和DC表面共刺激分子的表达呈平行。其有关基因位点分别于第6和第13号染色体上。展开更多
Given the protective roles of 25-hydroxyvitamin D (25(OH)D or vitamin D) in musculoskeletal health and the potential beneficial effects of vitamin D supplementation in reducing the risk of various chronic diseases, in...Given the protective roles of 25-hydroxyvitamin D (25(OH)D or vitamin D) in musculoskeletal health and the potential beneficial effects of vitamin D supplementation in reducing the risk of various chronic diseases, intensive repletion of vitamin D has been widely advocated. Of note, CD8 T cells have the highest levels of the vitamin D receptor compared with other major immune cells. The effects of vitamin D on CD8 T cells during aging, however, remain unclear. This study determined the relationship between vitamin D levels and CD8 T cell status in 34 healthy female subjects (all >60 years old). The CD8 T-cell phenotype was defined by the surface expression of CD28 and CD95. The low-25(OH)D serum groups (≤30 ng/ml) had higher percentages of CD28+CD95–CD8+ (na?ve) T cells and lower percentages of CD28+CD95+CD8+ (effector) T cells. By contrast, subjects with high levels of 25(OH)D had very low percentages of na?ve CD8 T cells but very high percentages of effector CD8 T cells. There was a significant inverse correlation between 25(OH)D levels and the frequency of na?ve CD8 T cells. The results show that higher levels of vitamin D are correlated with decreased frequencies of na?ve CD8 T cells during early aging, suggesting that higher levels of 25(OH)D accelerate CD8 T cell senescence. These results warrant further evaluation of the effects of vitamin D supplementation in immune aging.展开更多
文摘目的:探讨DC的抗肿瘤免疫功能是否与其基因位点有关。方法:选择19株基因型明确的BXD纯系小鼠的树突状细胞(DC),MTT法和ELISA分析对DC吞噬肿瘤细胞的作用;肿瘤细胞诱导:DC产生IL-12的能力;经肿瘤细胞刺激后的DC对T细胞增殖和:IFN-γ分泌的影响进行测定。用流式细胞仪检测:DC表面抗原标志CD80和CD54。有关数据用MapManagerQTX and WebQTL软件对DC抗肿瘤免疫的基因位点进行分析。结果:DC刺激T细胞增殖的作用很大程度取决于BXD各系的遗传差异。DC吞噬肿瘤细胞及诱导IL-12产生的能力都与DC刺激T细胞的增殖相一致(P<0.01)。基因位点定量分析(QTL)分析在第6和第13号染色体上有2个位点可能与DC的肿瘤抗原提呈加工功能和T细胞毒作用有关。结论:不同系的BXD小鼠,其DC的抗原提呈、加工能力不同。DC对肿瘤细胞生长的抑制与IL-12的诱导产生和DC表面共刺激分子的表达呈平行。其有关基因位点分别于第6和第13号染色体上。
基金National Institute of HealthDeep South Resource Center for Minority Aging Research (1P30AG031054-01, provided by the National Institute on Aging)
文摘Given the protective roles of 25-hydroxyvitamin D (25(OH)D or vitamin D) in musculoskeletal health and the potential beneficial effects of vitamin D supplementation in reducing the risk of various chronic diseases, intensive repletion of vitamin D has been widely advocated. Of note, CD8 T cells have the highest levels of the vitamin D receptor compared with other major immune cells. The effects of vitamin D on CD8 T cells during aging, however, remain unclear. This study determined the relationship between vitamin D levels and CD8 T cell status in 34 healthy female subjects (all >60 years old). The CD8 T-cell phenotype was defined by the surface expression of CD28 and CD95. The low-25(OH)D serum groups (≤30 ng/ml) had higher percentages of CD28+CD95–CD8+ (na?ve) T cells and lower percentages of CD28+CD95+CD8+ (effector) T cells. By contrast, subjects with high levels of 25(OH)D had very low percentages of na?ve CD8 T cells but very high percentages of effector CD8 T cells. There was a significant inverse correlation between 25(OH)D levels and the frequency of na?ve CD8 T cells. The results show that higher levels of vitamin D are correlated with decreased frequencies of na?ve CD8 T cells during early aging, suggesting that higher levels of 25(OH)D accelerate CD8 T cell senescence. These results warrant further evaluation of the effects of vitamin D supplementation in immune aging.
