Background:Cisplatin triggers Gasdermin E(GSDME)cleavage,causing membrane bubble formation,content release,and inflammation.Caspase-3 activation initiates GSDME cleavage,and thus inhibiting this pathway mitigates cisp...Background:Cisplatin triggers Gasdermin E(GSDME)cleavage,causing membrane bubble formation,content release,and inflammation.Caspase-3 activation initiates GSDME cleavage,and thus inhibiting this pathway mitigates cisplatin-induced pyroptosis in hepatocytes.This study aimed to delve into how cisplatin induces liver injury via pyroptosis.Methods:For animal experiments,C57BL/6J mice were divided into three groups:control,liver injury model group,and Ac-DMLD-CMK(caspase-3 inhibitor)intervention group.The liver histology was evaluated by hematoxylin and eosin staining,immunohistochemistry,immunofluorescence and TUNEL staining.The mRNA and protein levels were detected by real-time polymerase chain reaction(PCR)and Western blot analysis.For in vitro experiments,HL-7702 cells were treated with cisplatin or GSDME siRNA.Cell pyroptosis was determined via cellular morphology,cytotoxicity and viability detection,flow cytometric assay,and Western blot detection for the expression of pyroptosis-related proteins.Results:Cisplatin-induced distinct liver morphological changes,hepatocellular injury,and inflammation in mice,along with elevated serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)levels and increased pro-inflammatory cytokine expression.Heightened macrophage infiltration and hepatocellular death indicated cisplatin-induced hepatotoxicity.Cisplatin upregulated GSDME activation,along with Bax-mediated caspase-3 cleavage both in vivo and in vitro,implicating caspase-3/GSDME-dependent pyroptosis in liver injury.Treatment with Ac-DMLD-CMK ameliorated cisplatin-induced liver injury,reducing hepatocellular lesions,serum ALT and AST levels,cytokine expression,macrophage infiltration,and hepatocyte death.Ac-DMLD-CMK also attenuated GSDME-dependent pyroptosis post-cisplatin induction,as evidenced by decreased GSDME expression,Bax upregulation,and cleaved caspase-3 activation.For HL-7702 cells,GSDME siRNA transfection reduced GSDME expression,attenuated typical signs of cisplatin-induced pyroptosis,partially restored cell viability,and significantly inhibited cytotoxicity and a decrease in the proportion of propidium iodide-positive cells,indicating protection against cisplatininduced hepatocyte pyroptosis.Conclusions:Our study underscores the role of the caspase-3/GSDME signaling pathway in mediating cisplatin-induced hepatotoxicity,particularly in cases of excessive or cumulative cisplatin exposure.These findings suggest that targeting GSDME could represent a promising therapeutic approach to mitigate cisplatin-induced liver damage.展开更多
Objective： Stanniocalcin-1 （STC-1）, a kind of glycoprotein hormone, is universally up-regulated in various tumor tissues compared to corresponding normal tissues, suggesting it may be used as a tumor marker, whilst...Objective： Stanniocalcin-1 （STC-1）, a kind of glycoprotein hormone, is universally up-regulated in various tumor tissues compared to corresponding normal tissues, suggesting it may be used as a tumor marker, whilst disseminated tumor cells usually exist in peripheral blood. The aim of this study is to investigate the mRNA expression STC-1 in peripheral blood of colorectal cancer （CRC） and analyze its clinicopathological significance. Methods： The peripheral blood mononuclear cells （PBMNCs） were isolated from 78 CRC patients and 33 cancer-free controls. The expression status of STC-1 mRNA in PBMNCs was assessed by RT-PCR, its correlation with clinicopathological parameters and 5-year overall survival was analyzed as well. Results： In the 78 blood samples from CRC patients, 33 （42.31%） showed positive expression of STC-1 mRNA, and all of 15 gastrointestinal tumor tissues were positive for STC-1 mRNA. In contrast, all the blood samples from 14 healthy donors and 19 patients with inflammatory gastrointestinal disease were negative. Furthermore, STC-1 mRNA expression status was associated with patients’ advanced stage, distant metastasis and shortened overall survival. Conclusion： The detection of STC-1 mRNA in peripheral blood by RT-PCR was highly sensitive and specific for the patients with CRC. STC-1 mRNA may be a potential biomarker for detecting tumor micrometastasis and predicting prognosis.展开更多
BACKGROUND Surgical site infection(SSI)is one of the most common complications after gastric cancer(GC)surgery.The occurrence of SSI can lead to a prolonged postoperative hospital stay and increased medical expenses,a...BACKGROUND Surgical site infection(SSI)is one of the most common complications after gastric cancer(GC)surgery.The occurrence of SSI can lead to a prolonged postoperative hospital stay and increased medical expenses,and it can also affect postoperative rehabilitation and the quality of life of patients.Subcutaneous fat thickness(SFT)and abdominal depth(AD)can be used as predictors of SSI in patients undergoing radical resection of GC.AIM To explore the potential relationship between SFT or AD and SSI in patients undergoing elective radical resection of GC.METHODS Demographic,clinical,and pre-and intraoperative information of 355 patients who had undergone elective radical resection of GC were retrospectively collected from hospital electronic medical records.Univariate analysis was performed to screen out the significant parameters,which were subsequently analyzed using binary logistic regression and receiver-operating characteristic curve analysis.RESULTS The prevalence of SSI was 11.27%(40/355).Multivariate analyses revealed that SFT[odds ratio(OR)=1.150;95%confidence interval(95%CI):1.090-1.214;P<0.001],AD(OR=1.024;95%CI:1.009-1.040;P=0.002),laparoscopic-assisted surgery(OR=0.286;95%CI:0.030-0.797;P=0.017),and operation time(OR=1.008;95%CI:1.001-1.015;P=0.030)were independently associated with the incidence of SSI after elective radical resection of GC.In addition,the product of SFT and AD was a better potential predictor of SSI in these patients than either SFT or AD alone.CONCLUSION SFT and AD are independent risk factors and can be used as predictors of SSI in patients undergoing radical resection of GC.展开更多
Objective: Deleted in liver cancer 1 (DLC1) is a new candidate tumor suppressor gene, whose down-regulation or even silence will result from promoter hypermethylation in various human cancers including colorectal canc...Objective: Deleted in liver cancer 1 (DLC1) is a new candidate tumor suppressor gene, whose down-regulation or even silence will result from promoter hypermethylation in various human cancers including colorectal cancer (CRC). The aim of this study is to evaluate the diagnostic role of DLC1 gene methylation in the serum DNA from CRC patients. Methods: This study enrolled 85 CRC patients and 45 patients with benign colorectal diseases. Methylation-specific polymerase chain reaction (MSP) was used to determine the promoter methylation status of DLC1 gene in serum DNA. The combination of DLC1 methylation and conventional tumor markers was further analyzed. Results: Hypermethylation of DLC1 was detected in 42.4% (36/85) of CRC serums, while seldom in the benign controls (8.9%, 4/45) (P<0.001). The aberrant DLC1 methylation in serum DNA was not associated with patients’ clinicopathological features and elevated CEA/CA19-9 levels. Furthermore, the combinational analysis of CEA, CA19-9 and DLC1 methylation showed a higher sensitivity and no reduced diagnostic specificity than CEA and CA19-9 combination for CRC diagnosis. Conclusion: The serum DLC1 methylation may be a promising biomarker for the early detection of CRC, which will further increase the diagnostic efficiency in combination with CEA and CA19-9.展开更多
Objective To review and explore the advantages and disadvantages of the teaching of the reproductive system in Shanghai Jiao Tong University School of Medicine,in order to provide a feasible scheme for the subsequent ...Objective To review and explore the advantages and disadvantages of the teaching of the reproductive system in Shanghai Jiao Tong University School of Medicine,in order to provide a feasible scheme for the subsequent reform of the teaching system,including the syllabus design and the optimization of the teaching method,etc.Methods With the one-year practice in the teaching of the reproductive system,this paper has explored the achievements in the implementation of this teaching system from three aspects,including the teaching experience and feelings of teachers,student questionnaires and test scores.Meanwhile,this paper has also deeply analyzed some problems that may exist or be derived from the teaching practice in that teaching model.Results The new teaching method has huge differences from the traditional"discipline-oriented"teaching method in the curricular design,teachers’lecture form,students’learning skills and examination types,etc.What is more important,the new teaching method will significantly renew the concepts of both teaching and learning.Conclusion The method of the systemic teaching is one of the critical ways to realize the reform of the medical education,and can also provide the necessary basis for the cultivation of professional high-quality teachers required in the medical education.展开更多
A novel and fluorescence retention inverse opal has been achieved from organosilane-polymerized carbon dots（SiCDs）, which is prepared via infiltrating SiCD solution into the interstice of photonic crystal（PC） temp...A novel and fluorescence retention inverse opal has been achieved from organosilane-polymerized carbon dots（SiCDs）, which is prepared via infiltrating SiCD solution into the interstice of photonic crystal（PC） template, low temperature treatment, heating polymerization and removing the colloidal template. The as-prepared SiCD inverse opals demonstrate close-cell structure, which is completely different from conventional open-cell structure. Then the fluorescence signal of as-prepared sample keeps almost unchanged in CuCl suspension while the fluorescence of SiCD solution can be quenched by CuCl suspension through an effective electron transfer process. This phenomenon can be attributed to the combined effect of high hydrostatic pressure in the pore structure, stable crosslinking network and fluorescence enhancement by PC structure. The SiCD inverse opals have advantages of unique close-cell structure, easy preparation and good repeatability that give an important insight into the design and manufacture of novel and advanced optical devices.展开更多
As a typical biometric cue with great diversities, smile is a fairly influential signal in social interaction, which reveals the emotional feeling and inner state of a person. Spontaneous and posed smiles initiated by...As a typical biometric cue with great diversities, smile is a fairly influential signal in social interaction, which reveals the emotional feeling and inner state of a person. Spontaneous and posed smiles initiated by different brain systems have differences in both morphology and dynamics. Distinguishing the two types of smiles remains challenging as discriminative subtle changes need to be captured, which are also uneasily observed by human eyes. Most previous related works about spontaneous versus posed smile recognition concentrate on extracting geometric features while appearance features are not fully used, leading to the loss of texture information. In this paper, we propose a region-specific texture descriptor to represent local pattern changes of different facial regions and compensate for limitations of geometric features. The temporal phase of each facial region is divided by calculating the intensity of the corresponding facial region rather than the intensity of only the mouth region. A mid-level fusion strategy of support vector machine is employed to combine the two feature types. Experimental results show that both our proposed appearance representation and its combination with geometry-based facial dynamics achieve favorable performances on four baseline databases： BBC, SPOS, MMI, and UvA-NEMO.展开更多
Previous studies have indicated that the peak of the quarkonium entropy at the deconfinement transition can be related to the entropic force,which would induce the dissociation of heavy quarkonium.In this study,we inv...Previous studies have indicated that the peak of the quarkonium entropy at the deconfinement transition can be related to the entropic force,which would induce the dissociation of heavy quarkonium.In this study,we investigated the entropic force in a rotating hot and dense medium using AdS/CFT correspondence.It was found that the inclusion of angular velocity increases the entropic force,thus enhancing quarkonium dissociation,while chemical potential has the same effect.The results imply that the quarkonium dissociates easier in rotating medium compared with the static case.展开更多
基金supported by grants from the National Natural Science Foundation of China(8170060495 and 82170682)。
文摘Background:Cisplatin triggers Gasdermin E(GSDME)cleavage,causing membrane bubble formation,content release,and inflammation.Caspase-3 activation initiates GSDME cleavage,and thus inhibiting this pathway mitigates cisplatin-induced pyroptosis in hepatocytes.This study aimed to delve into how cisplatin induces liver injury via pyroptosis.Methods:For animal experiments,C57BL/6J mice were divided into three groups:control,liver injury model group,and Ac-DMLD-CMK(caspase-3 inhibitor)intervention group.The liver histology was evaluated by hematoxylin and eosin staining,immunohistochemistry,immunofluorescence and TUNEL staining.The mRNA and protein levels were detected by real-time polymerase chain reaction(PCR)and Western blot analysis.For in vitro experiments,HL-7702 cells were treated with cisplatin or GSDME siRNA.Cell pyroptosis was determined via cellular morphology,cytotoxicity and viability detection,flow cytometric assay,and Western blot detection for the expression of pyroptosis-related proteins.Results:Cisplatin-induced distinct liver morphological changes,hepatocellular injury,and inflammation in mice,along with elevated serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)levels and increased pro-inflammatory cytokine expression.Heightened macrophage infiltration and hepatocellular death indicated cisplatin-induced hepatotoxicity.Cisplatin upregulated GSDME activation,along with Bax-mediated caspase-3 cleavage both in vivo and in vitro,implicating caspase-3/GSDME-dependent pyroptosis in liver injury.Treatment with Ac-DMLD-CMK ameliorated cisplatin-induced liver injury,reducing hepatocellular lesions,serum ALT and AST levels,cytokine expression,macrophage infiltration,and hepatocyte death.Ac-DMLD-CMK also attenuated GSDME-dependent pyroptosis post-cisplatin induction,as evidenced by decreased GSDME expression,Bax upregulation,and cleaved caspase-3 activation.For HL-7702 cells,GSDME siRNA transfection reduced GSDME expression,attenuated typical signs of cisplatin-induced pyroptosis,partially restored cell viability,and significantly inhibited cytotoxicity and a decrease in the proportion of propidium iodide-positive cells,indicating protection against cisplatininduced hepatocyte pyroptosis.Conclusions:Our study underscores the role of the caspase-3/GSDME signaling pathway in mediating cisplatin-induced hepatotoxicity,particularly in cases of excessive or cumulative cisplatin exposure.These findings suggest that targeting GSDME could represent a promising therapeutic approach to mitigate cisplatin-induced liver damage.
基金supported by the grants from the Department of Health, Jiangsu Province, China (No.H200116)
文摘Objective： Stanniocalcin-1 （STC-1）, a kind of glycoprotein hormone, is universally up-regulated in various tumor tissues compared to corresponding normal tissues, suggesting it may be used as a tumor marker, whilst disseminated tumor cells usually exist in peripheral blood. The aim of this study is to investigate the mRNA expression STC-1 in peripheral blood of colorectal cancer （CRC） and analyze its clinicopathological significance. Methods： The peripheral blood mononuclear cells （PBMNCs） were isolated from 78 CRC patients and 33 cancer-free controls. The expression status of STC-1 mRNA in PBMNCs was assessed by RT-PCR, its correlation with clinicopathological parameters and 5-year overall survival was analyzed as well. Results： In the 78 blood samples from CRC patients, 33 （42.31%） showed positive expression of STC-1 mRNA, and all of 15 gastrointestinal tumor tissues were positive for STC-1 mRNA. In contrast, all the blood samples from 14 healthy donors and 19 patients with inflammatory gastrointestinal disease were negative. Furthermore, STC-1 mRNA expression status was associated with patients’ advanced stage, distant metastasis and shortened overall survival. Conclusion： The detection of STC-1 mRNA in peripheral blood by RT-PCR was highly sensitive and specific for the patients with CRC. STC-1 mRNA may be a potential biomarker for detecting tumor micrometastasis and predicting prognosis.
基金The Nanjing Health Science and Technology Development Fund Project,No.YKK18241.
文摘BACKGROUND Surgical site infection(SSI)is one of the most common complications after gastric cancer(GC)surgery.The occurrence of SSI can lead to a prolonged postoperative hospital stay and increased medical expenses,and it can also affect postoperative rehabilitation and the quality of life of patients.Subcutaneous fat thickness(SFT)and abdominal depth(AD)can be used as predictors of SSI in patients undergoing radical resection of GC.AIM To explore the potential relationship between SFT or AD and SSI in patients undergoing elective radical resection of GC.METHODS Demographic,clinical,and pre-and intraoperative information of 355 patients who had undergone elective radical resection of GC were retrospectively collected from hospital electronic medical records.Univariate analysis was performed to screen out the significant parameters,which were subsequently analyzed using binary logistic regression and receiver-operating characteristic curve analysis.RESULTS The prevalence of SSI was 11.27%(40/355).Multivariate analyses revealed that SFT[odds ratio(OR)=1.150;95%confidence interval(95%CI):1.090-1.214;P<0.001],AD(OR=1.024;95%CI:1.009-1.040;P=0.002),laparoscopic-assisted surgery(OR=0.286;95%CI:0.030-0.797;P=0.017),and operation time(OR=1.008;95%CI:1.001-1.015;P=0.030)were independently associated with the incidence of SSI after elective radical resection of GC.In addition,the product of SFT and AD was a better potential predictor of SSI in these patients than either SFT or AD alone.CONCLUSION SFT and AD are independent risk factors and can be used as predictors of SSI in patients undergoing radical resection of GC.
文摘Objective: Deleted in liver cancer 1 (DLC1) is a new candidate tumor suppressor gene, whose down-regulation or even silence will result from promoter hypermethylation in various human cancers including colorectal cancer (CRC). The aim of this study is to evaluate the diagnostic role of DLC1 gene methylation in the serum DNA from CRC patients. Methods: This study enrolled 85 CRC patients and 45 patients with benign colorectal diseases. Methylation-specific polymerase chain reaction (MSP) was used to determine the promoter methylation status of DLC1 gene in serum DNA. The combination of DLC1 methylation and conventional tumor markers was further analyzed. Results: Hypermethylation of DLC1 was detected in 42.4% (36/85) of CRC serums, while seldom in the benign controls (8.9%, 4/45) (P<0.001). The aberrant DLC1 methylation in serum DNA was not associated with patients’ clinicopathological features and elevated CEA/CA19-9 levels. Furthermore, the combinational analysis of CEA, CA19-9 and DLC1 methylation showed a higher sensitivity and no reduced diagnostic specificity than CEA and CA19-9 combination for CRC diagnosis. Conclusion: The serum DLC1 methylation may be a promising biomarker for the early detection of CRC, which will further increase the diagnostic efficiency in combination with CEA and CA19-9.
文摘Objective To review and explore the advantages and disadvantages of the teaching of the reproductive system in Shanghai Jiao Tong University School of Medicine,in order to provide a feasible scheme for the subsequent reform of the teaching system,including the syllabus design and the optimization of the teaching method,etc.Methods With the one-year practice in the teaching of the reproductive system,this paper has explored the achievements in the implementation of this teaching system from three aspects,including the teaching experience and feelings of teachers,student questionnaires and test scores.Meanwhile,this paper has also deeply analyzed some problems that may exist or be derived from the teaching practice in that teaching model.Results The new teaching method has huge differences from the traditional"discipline-oriented"teaching method in the curricular design,teachers’lecture form,students’learning skills and examination types,etc.What is more important,the new teaching method will significantly renew the concepts of both teaching and learning.Conclusion The method of the systemic teaching is one of the critical ways to realize the reform of the medical education,and can also provide the necessary basis for the cultivation of professional high-quality teachers required in the medical education.
基金financially supported by the Ministry of Science and Technology of China (Nos.2016YFA0200803 and 2016YFB0402004)the National Natural Science Foundation of China (Nos.51673207 and 51373183)
文摘A novel and fluorescence retention inverse opal has been achieved from organosilane-polymerized carbon dots（SiCDs）, which is prepared via infiltrating SiCD solution into the interstice of photonic crystal（PC） template, low temperature treatment, heating polymerization and removing the colloidal template. The as-prepared SiCD inverse opals demonstrate close-cell structure, which is completely different from conventional open-cell structure. Then the fluorescence signal of as-prepared sample keeps almost unchanged in CuCl suspension while the fluorescence of SiCD solution can be quenched by CuCl suspension through an effective electron transfer process. This phenomenon can be attributed to the combined effect of high hydrostatic pressure in the pore structure, stable crosslinking network and fluorescence enhancement by PC structure. The SiCD inverse opals have advantages of unique close-cell structure, easy preparation and good repeatability that give an important insight into the design and manufacture of novel and advanced optical devices.
基金the National Natural Science Foundation of China （No. 60675025）, the National High-Tech R＆D Program （863） of China （No. 2006AA04Z247）, the Scientific and Tech- nical Innovation Commission of Shenzhen Municipality, China （Nos. JCYJ20130331144631730 and JCYJ20130331144716089）, and the Specialized Research Fund for the Doctoral Program of Higher Education, China （No. 20130001110011）
文摘As a typical biometric cue with great diversities, smile is a fairly influential signal in social interaction, which reveals the emotional feeling and inner state of a person. Spontaneous and posed smiles initiated by different brain systems have differences in both morphology and dynamics. Distinguishing the two types of smiles remains challenging as discriminative subtle changes need to be captured, which are also uneasily observed by human eyes. Most previous related works about spontaneous versus posed smile recognition concentrate on extracting geometric features while appearance features are not fully used, leading to the loss of texture information. In this paper, we propose a region-specific texture descriptor to represent local pattern changes of different facial regions and compensate for limitations of geometric features. The temporal phase of each facial region is divided by calculating the intensity of the corresponding facial region rather than the intensity of only the mouth region. A mid-level fusion strategy of support vector machine is employed to combine the two feature types. Experimental results show that both our proposed appearance representation and its combination with geometry-based facial dynamics achieve favorable performances on four baseline databases： BBC, SPOS, MMI, and UvA-NEMO.
文摘Previous studies have indicated that the peak of the quarkonium entropy at the deconfinement transition can be related to the entropic force,which would induce the dissociation of heavy quarkonium.In this study,we investigated the entropic force in a rotating hot and dense medium using AdS/CFT correspondence.It was found that the inclusion of angular velocity increases the entropic force,thus enhancing quarkonium dissociation,while chemical potential has the same effect.The results imply that the quarkonium dissociates easier in rotating medium compared with the static case.
