Vanillin(V)is an aromatic compound recognized for its diverse bioactivities;however,its practical applications are limited by its inherent instability.In this study,thermosensitive microspheres comprising PHBV(Poly(-3...Vanillin(V)is an aromatic compound recognized for its diverse bioactivities;however,its practical applications are limited by its inherent instability.In this study,thermosensitive microspheres comprising PHBV(Poly(-3-hydroxybutyrate-co-3-hydroxyvalerate)),PEG(Polyethylene glycol),and vanillin(PVP)were developed to improve the stability of vanillin,followed by structural and bioactivity evaluations.Scanning electron microscopy(SEM),Fourier-transform infrared spectroscopy(FT-IR),and differential scanning calorimetry(DSC)confirmed the successful encapsulation of vanillin within the microspheres.Thermogravimetric analysis(TGA)revealed that the PVP microspheres exhibited significant thermal stability up to 420◦C.Furthermore,the bioactivity of PVP was evaluated at a concentration of 200 mg/mL.The results demonstrated that PVP significantly inhibited lipopolysaccharide(LPS)-induced production of nitric oxide(NO)by 40.38%and reactive oxygen species(ROS)by 50.47%,indicating strong antibacterial properties,with inhibition rates against Escherichia coli and Staphylococcus aureus of approximately 77%and 58%,respectively.Overall,the findings suggest that this method offers a highly promising intelligent delivery system for vanillin,significantly enhancing its bioactivity.展开更多
文摘Vanillin(V)is an aromatic compound recognized for its diverse bioactivities;however,its practical applications are limited by its inherent instability.In this study,thermosensitive microspheres comprising PHBV(Poly(-3-hydroxybutyrate-co-3-hydroxyvalerate)),PEG(Polyethylene glycol),and vanillin(PVP)were developed to improve the stability of vanillin,followed by structural and bioactivity evaluations.Scanning electron microscopy(SEM),Fourier-transform infrared spectroscopy(FT-IR),and differential scanning calorimetry(DSC)confirmed the successful encapsulation of vanillin within the microspheres.Thermogravimetric analysis(TGA)revealed that the PVP microspheres exhibited significant thermal stability up to 420◦C.Furthermore,the bioactivity of PVP was evaluated at a concentration of 200 mg/mL.The results demonstrated that PVP significantly inhibited lipopolysaccharide(LPS)-induced production of nitric oxide(NO)by 40.38%and reactive oxygen species(ROS)by 50.47%,indicating strong antibacterial properties,with inhibition rates against Escherichia coli and Staphylococcus aureus of approximately 77%and 58%,respectively.Overall,the findings suggest that this method offers a highly promising intelligent delivery system for vanillin,significantly enhancing its bioactivity.
