Objective The aim of this study was to investigate the expression of PTV1 lncRNA in gliomas and themechanism of its interaction with miR-203a.Methods U87 and U251 cells were cultured stably and transfected with sh-PTV...Objective The aim of this study was to investigate the expression of PTV1 lncRNA in gliomas and themechanism of its interaction with miR-203a.Methods U87 and U251 cells were cultured stably and transfected with sh-PTV1 or ov-PTV1, respectively.The proliferative activity of U87 and U251 cells was detected and the transplanted tumor model nude micewere divided into U87 and U251 groups. U87-sh and u251-ov cells were injected into the armpit, thenmiR-203a mic and miR-203a inhibitors were administered to detect the changes in the expression of tumorrelatedproteins.Results The relative expression of PTV1 in gliomas was significantly higher than that in normal braintissues, while in GBM it was significantly higher than that in low-grade gliomas. Knockdown of PTV1significantly inhibited the proliferation of U87 cells, resulting in fewer cell clones;overexpression of rPTV1significantly promoted the proliferation of U251 cells, resulting in more cell colonies. The dual LuciferaseReporter assay showed that SP2 was a potential target of miR-203a. When U87 cells were treated with amiR-203a mimic, the expression of SP2 decreased;and when U251 cells were treated with a miR-203ainhibitor, the expression of SP2 increased significantly. SP2 was overexpressed in u87-sh cells and theproliferation, migration, and invasion of u87-sh cells were significantly enhanced. U251-ov cells showedthe opposite trend. Compared with the control group mice, the tumor volume in u87-sh group mice wassignificantly smaller and the positive rate of SP2 in tumor tissue was significantly lower. After administrationof the miR-203a inhibitor, the tumor volume increased gradually and the positive rate of SP2 increasedsignificantly, while u251-ov mice showed the opposite trend.Conclusion lncRNA PTV1 can be used as a molecule to interfere with miR-203a expression in order todownregulate SP2 and to promote the proliferation and invasion of glioma cells. lncRNA PTV1 may be anew biomarker and therapeutic target for glioma.展开更多
Hydrogen generated by water electrolysis is considered as one of the most promising protocols to partly replace the roles of traditional fossil fuels.However,high‐performance electrocatalyst satisfied with the indust...Hydrogen generated by water electrolysis is considered as one of the most promising protocols to partly replace the roles of traditional fossil fuels.However,high‐performance electrocatalyst satisfied with the industrial requirement still faces significant challenges.Low‐temperature plasma contains numerous high‐energy ions,electrons and other reactive species,which can provide a highly reactive environment for tuning the physio‐chemical structures of catalysts through plasma milling,etching,doping and/or deposi-tion.It is well‐known that high‐temperature micro‐filaments contained in plasmas can cause some special modifications of the catalyst surface,thus effectively adjusting the physio‐chemical structure of latterly engineered compounds.Therefore,low‐temperature plasma technologies,especially the dielectric barrier discharge(DBD)and radio frequency(RF)plasmas,can be considered as a green and sustainable strategy for engineering high‐performance electrocatalysts for water splitting(hydrogen evolution reaction[HER];oxygen evolution reaction[OER]).Herein,recent progress of DBD and RF plasmas for fabricating and modifying transition metal‐based electrocatalysts(e.g.sulphide,phos-phide,selenide,oxide,hydroxide)for hydrogen evolution reaction or OER is compre-hensively reviewed,and the role of plasma is also discussed.展开更多
基金Supported by a grant from the general program of NSFC(No.81472965)。
文摘Objective The aim of this study was to investigate the expression of PTV1 lncRNA in gliomas and themechanism of its interaction with miR-203a.Methods U87 and U251 cells were cultured stably and transfected with sh-PTV1 or ov-PTV1, respectively.The proliferative activity of U87 and U251 cells was detected and the transplanted tumor model nude micewere divided into U87 and U251 groups. U87-sh and u251-ov cells were injected into the armpit, thenmiR-203a mic and miR-203a inhibitors were administered to detect the changes in the expression of tumorrelatedproteins.Results The relative expression of PTV1 in gliomas was significantly higher than that in normal braintissues, while in GBM it was significantly higher than that in low-grade gliomas. Knockdown of PTV1significantly inhibited the proliferation of U87 cells, resulting in fewer cell clones;overexpression of rPTV1significantly promoted the proliferation of U251 cells, resulting in more cell colonies. The dual LuciferaseReporter assay showed that SP2 was a potential target of miR-203a. When U87 cells were treated with amiR-203a mimic, the expression of SP2 decreased;and when U251 cells were treated with a miR-203ainhibitor, the expression of SP2 increased significantly. SP2 was overexpressed in u87-sh cells and theproliferation, migration, and invasion of u87-sh cells were significantly enhanced. U251-ov cells showedthe opposite trend. Compared with the control group mice, the tumor volume in u87-sh group mice wassignificantly smaller and the positive rate of SP2 in tumor tissue was significantly lower. After administrationof the miR-203a inhibitor, the tumor volume increased gradually and the positive rate of SP2 increasedsignificantly, while u251-ov mice showed the opposite trend.Conclusion lncRNA PTV1 can be used as a molecule to interfere with miR-203a expression in order todownregulate SP2 and to promote the proliferation and invasion of glioma cells. lncRNA PTV1 may be anew biomarker and therapeutic target for glioma.
基金supported by the National Natural Science Foundation of China(52177162)the National Science Fund for Distinguished Young Scholars(51925703)+1 种基金the Zhejiang Natural Science Foundations of China(LZ22E070003)the State Key Laboratory of Alternate Electrical Power System with Renewable Energy Sources(LAPS22018).
文摘Hydrogen generated by water electrolysis is considered as one of the most promising protocols to partly replace the roles of traditional fossil fuels.However,high‐performance electrocatalyst satisfied with the industrial requirement still faces significant challenges.Low‐temperature plasma contains numerous high‐energy ions,electrons and other reactive species,which can provide a highly reactive environment for tuning the physio‐chemical structures of catalysts through plasma milling,etching,doping and/or deposi-tion.It is well‐known that high‐temperature micro‐filaments contained in plasmas can cause some special modifications of the catalyst surface,thus effectively adjusting the physio‐chemical structure of latterly engineered compounds.Therefore,low‐temperature plasma technologies,especially the dielectric barrier discharge(DBD)and radio frequency(RF)plasmas,can be considered as a green and sustainable strategy for engineering high‐performance electrocatalysts for water splitting(hydrogen evolution reaction[HER];oxygen evolution reaction[OER]).Herein,recent progress of DBD and RF plasmas for fabricating and modifying transition metal‐based electrocatalysts(e.g.sulphide,phos-phide,selenide,oxide,hydroxide)for hydrogen evolution reaction or OER is compre-hensively reviewed,and the role of plasma is also discussed.
