AIM: To establish the methylation profile of the promoter CpG islands of 31 genes that might play etiological roles in colon carcinogenesis.METHODS: The methylation specific PCR in conjunction of sequendng verificatio...AIM: To establish the methylation profile of the promoter CpG islands of 31 genes that might play etiological roles in colon carcinogenesis.METHODS: The methylation specific PCR in conjunction of sequendng verification was used to establish the methylationprofile of the promoter CpG islands of 31 genes in colorectal cancer (n = 65), the neighboring non-cancerous tissues (n = 5), colorectal adenoma (n = 8), and normal mucosa (n = 1). Immunohistochemically, expression of 10 genes was assessed on the home-made tissue microarrays of tissues from 58 patients. The correlation of tumor specific changes with each of clinical-pathologic features was scrutinized with relevant statistic tools.RESULTS: In comparison with the normal mucosa of the non-cancer patients, the following 14 genes displayed no tumor associated changes: breast cancer 1, early onset (BRCA1), cadherin 1, type 1, E-cadherin (epithelial) (CDH1),death-associated protein kinase 1 (DAPK1), DNA (cytosine-5-)-methyltransferase 1 (DNMT1), melanoma antigen, family A, 1 (directs expression of antigen MZ2-E) (MAGEA1), tumor suppressor candidate 3 (N33), cyclin-dependent kinase inhibitor 1A (p21, Cipl) (p21^WAF1), cyclin-dependent kinase inhibitor 1B (p27, 10pl) (p27^WAF1), phosphatase and tensin hornolog (mutated in multiple advanced cancers 1) (PTEN), retinoic acid receptor, beta (RAR-, Ras association (RaIGDS/AF-6) domain family 1 C (RASSFIC), secreted frizzled-related protein 1 (SFRP1), tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy, pseudoinfiammatory) (TIMP3),and von HippeI-Lindau syndrome (VHL). The rest 17 targets exhibited to various extents the tumor associated changes.As changes in methylation of the following genes occurred marginally, their impact on the formation of colorectal cancer were trivial: adenomatous polyposis coli (APC) (8%, 5165),Ras association (RaIGDS/AF-6) domain family 1A (RASSFIA) (3%, 2/65) and cyclin-dependent kinase inhibitor 2A,alternated reading frame Co14~) (6%, 4/65). The following genes exhibited moderate changes in rnethylation: O-6rnethylguanine-DNA rnethyltransferase (MGMT) (20%, 13/65),rnutL hornolog 1, colon cancer, nonpolyposis type 2 (E. coli) (hMLH1) (18%, 12/65), cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) P16^NK4a) (10%, 10/65),rnethylated in tumor 1 (MINT1) (15%, 10/65), methylated in tumor 31 (MINT31) (11%, 7/65). The rest changed greatly in the rnethylation pattern in colorectal cancer (CRC): cyclin A1 (cyclin al) (100%, 65/65), caudal type homeobox transcriptdon factor 1 (CDX1) (100%, 65/65), RAR(85%, 55/65), myogenic factor 3 (MYOD1) (69%, 45/65),cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)(p15^INK4b) (68%, 44/65), prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (COX2) (72%, 47/65), cadherin 13, H-cadherin (heart) (CDH13) (65%, 42/65), CAAX box 1 (OO~/) (58%, 38/65),tumor protein p73 (p73) (63%, 41/65) and Wilrns tumor 1 (WT/) (58%, 38/65). However, no significant correlation of changes in rnethylation with any given clinical-pathological features was detected. Furthermore, the frequent changes in rnethylation appeared to be an early phase event of colon carcinogenesis. The in situ expression of 10 genes was assessed by the irnrnunohistochernical approach at the protein level: CDH1, CDH13, COX2, cyclin A1, hMLH1,MGMT, p14^ARF, p73, RAR-, and TIMP3 genes in the context of the rnethylation status in colorectal cancer. No clear correlation between the hyperrnethylation of the promoter CpG islands and the negative expression of the genes was established.CONCLUSION: The methylation profile of 31 genes was established in patients with colon cancer and colorectal adenornas, which provides new insights into the DNA rnethylation mediated mechanisms underlying the carcinogenesis of colorectal cancer and may be of prognostic values for colorectal cancer.展开更多
AIM: To observe the expression of cyclooxygenase-2 (COX-2) and to investigate the association between COX-2expression and infection with cytotoxic-associated gene A( cagA) positive strair Helicobacter pylori ( Hp) in ...AIM: To observe the expression of cyclooxygenase-2 (COX-2) and to investigate the association between COX-2expression and infection with cytotoxic-associated gene A( cagA) positive strair Helicobacter pylori ( Hp) in humangastric cancer, and subsequently to provide fresh ideas forthe early prevention of gastric cancer.METHODS: 32 Specimens of gastric cancer andcorresponding adjacent normal gastric mucosa were obtainedfrom patients who had undergone surgical operations ofgastric cancer. All the samples including 1 case of stomachmalignant lymphoma and 31 cases of gastric adenocarcinomawere confirmed by pathology diagnosis. The expression ofCOX-2 in 32 specimens of gastric cancer and correspondingadjacent normal gastric mucosa was quantitativelydetermined and analyzed with Flow Cytometry, and the levelsof COX-2 protein were compared between specimens withcagA+ Hp infection and those without cagA+ Hp infection.The cagA gene in 32 specimens of gastric cancer wasdetected bypolymerase chain reaction (PCR) method.RESULTS: Twenty-seven of 32 (84 %) specimens of gastriccancer showed over-expression of COX-2, compared withthe adjacent normal gastric mucosa. cagA+ gene weredetected from 19 specimens of gastric cancer, but not fromthe other 13 specimens. The levels of COX-2 protein in 19specimens of gastric cancer with cagA+ Hp infection (thenumber of positive cells was 73.82±18.2) were significantlyhigher than those in the 13 specimens without cagA+ Hpinfection (the number of positive cells was 35.92±22.1).CONCLUSION: COX-2 is overexpressed in gastric cancerand cagA+Hp infection could up-regulate the expression ofCOX-2 in gastric cancer in human. There may also existanother way or channel to regulate the expression of COX-2 in gastric cancer in addition to cagA+Hp infection.Therefore, applying COX-2 selective inhibitors could be aneffective and promising way to prevent gastric cancer.展开更多
Mercury ions have been considered highly toxic to human health. What would be great is to develop the ionic probes without any toxicities themselves. Here, we report a friendly, highly sensitive mercury(II) ionic prob...Mercury ions have been considered highly toxic to human health. What would be great is to develop the ionic probes without any toxicities themselves. Here, we report a friendly, highly sensitive mercury(II) ionic probe, watersoluble photoluminescence carbon dots which were synthesized by simply hydrothermal treatment of fresh cherry tomatoes without adding any other reagents. The ultra-small(\1 nm) carbon dots show robust excitation-depended photoluminescence under a wide p H range(4–10) or a strong ionic strength of up to 1 M, and the detection limit of mercury(II) has been determined as low as 18 n M. We envision such water-soluble, biocompatible carbon dots that could be applied to biolabeling, bio-imaging, and biosensing fields.展开更多
The inlet temperature of the Vacuum Gas Oil(VGO)hydrotreating reactor of a refinery is analyzed with the integration of multiple series reactors and hydrogen network considered.The effect of the inlet temperature(T1)o...The inlet temperature of the Vacuum Gas Oil(VGO)hydrotreating reactor of a refinery is analyzed with the integration of multiple series reactors and hydrogen network considered.The effect of the inlet temperature(T1)on hydrogen sinks/sources and the product output is analyzed systematically based on the simulation of the series reactors,including VGO hydrotreating reactor,hydrocracking reactor,fluid catalytic cracking reactor and visbreaking reactor.The general relation between the Hydrogen Utility Adjustment(HUA)and multiple pairs of varying sinks and sources is deduced,and correlations between varying streams and T1 are linearly fitted.Based on this,the quantitative equation between HUA and T1 is derived,and corresponding diagram is constructed.The T1 corresponding the minimum hydrogen consumption is identified to be 345°C.展开更多
OBJECTIVE To analyze our management strategy and results of treating patients affected by meningiomas invading the sagittal or transverse sinuses.METHOOS Review of data from 35 patients with pathologically confirmed m...OBJECTIVE To analyze our management strategy and results of treating patients affected by meningiomas invading the sagittal or transverse sinuses.METHOOS Review of data from 35 patients with pathologically confirmed meningiomas(29 of the sagittal sinus and 6 of the transverse sinus)surgically treated between from July 1999 and June 2003,including clinical manifestations,mode of diagnosis and curative effect of microsurgery etc.For our surgical decision-making,meningiomas were classified into six types based on the degree of sinus involvement.RESULTS A Simpson's GradeⅠresection was achieved in 27 cases(77.1%),GradeⅡin 6(17.1%)and GradeⅢin 2(5.7%).No patients died after the operations.The recurrence rate in the study overall was 2.9%,with a follow-up period from 3 to 6 years.CONCLUSION Application of microsurgical techniques,protection of the sinus,avoidance of damages to the cerebral cortex,veins of the central sulcus,as well as other veins from the tumor,are the major factors for increasing the rate of total resection,reducing complications and improving the quality of life for the patients with meningiomas invading the sagittal or transverse sinuses.展开更多
The paper introduces a new class of numerical schemes for the approximate solutions of stochastic pantograph equations. As an effective technique to implement implicit stochastic methods, strong predictor-corrector me...The paper introduces a new class of numerical schemes for the approximate solutions of stochastic pantograph equations. As an effective technique to implement implicit stochastic methods, strong predictor-corrector methods （PCMs） are designed to handle scenario simulation of solutions of stochastic pantograph equations. It is proved that the PCMs are strong convergent with order 1/2.Linear M^-stabiiity of stochastic pantograph equationsand the PCMs are researched in the paper. Sufficient conditions of MS-unstability of stochastic pantograph equations and MS-stability of the PCMs are obtained, respectively. Numerical experiments demonstrate these theoretical results.展开更多
In this paper the homotopy continuation method for stochastic two-point boundary value problems driven by additive noises is studied. The existence of the solution of the homotopy equation is proved. Numerical schemes...In this paper the homotopy continuation method for stochastic two-point boundary value problems driven by additive noises is studied. The existence of the solution of the homotopy equation is proved. Numerical schemes are constructed and error estimates are obtained. Numerical experiments demonstrate the effectiveness of the homotopy continu- ation method over other commonly used methods such as the shooting method.展开更多
This paper aims to investigate sufficient conditions for the recovery of sparse signals via the orthogonal matching pursuit （OMP） algorithm. In the noiseless case, we present a novel sufficient condition for the exa...This paper aims to investigate sufficient conditions for the recovery of sparse signals via the orthogonal matching pursuit （OMP） algorithm. In the noiseless case, we present a novel sufficient condition for the exact recovery of all k-sparse signals by the OMP algorithm, and demonstrate that this condition is sharp. In the noisy case, a sufficient condition for recovering the support of k-sparse signal is also presented. Generally, the computation for the restricted isometry constant （RIC） in these sufficient conditions is typically difficult, therefore we provide a new condition which is not only computable but also sufficient for the exact recovery of all k-sparse signals.展开更多
The median follow-up of the malignant glioma patients who received surgical resection combined with radiochemotherapy is 16 months. The recurrence of malignant glioma is unavoidable. Although the patient with recurren...The median follow-up of the malignant glioma patients who received surgical resection combined with radiochemotherapy is 16 months. The recurrence of malignant glioma is unavoidable. Although the patient with recurrent glioblastoma has multiple choices of therapy, the 5-year survival rate is still less than 5%, and the average survival time is less than 12 months. Currently, there is no single second-line drug can have survival benefits.展开更多
基金Supported by the National High Technology Research and DevelopmentProgram of China (863 Program),No.2002AA2Z3352,the ScienceFoundation of Shanghai Municipal Government,No.02DJ14056,and the Special Fund set up by the State-Key Laboratory for Oncogenes
文摘AIM: To establish the methylation profile of the promoter CpG islands of 31 genes that might play etiological roles in colon carcinogenesis.METHODS: The methylation specific PCR in conjunction of sequendng verification was used to establish the methylationprofile of the promoter CpG islands of 31 genes in colorectal cancer (n = 65), the neighboring non-cancerous tissues (n = 5), colorectal adenoma (n = 8), and normal mucosa (n = 1). Immunohistochemically, expression of 10 genes was assessed on the home-made tissue microarrays of tissues from 58 patients. The correlation of tumor specific changes with each of clinical-pathologic features was scrutinized with relevant statistic tools.RESULTS: In comparison with the normal mucosa of the non-cancer patients, the following 14 genes displayed no tumor associated changes: breast cancer 1, early onset (BRCA1), cadherin 1, type 1, E-cadherin (epithelial) (CDH1),death-associated protein kinase 1 (DAPK1), DNA (cytosine-5-)-methyltransferase 1 (DNMT1), melanoma antigen, family A, 1 (directs expression of antigen MZ2-E) (MAGEA1), tumor suppressor candidate 3 (N33), cyclin-dependent kinase inhibitor 1A (p21, Cipl) (p21^WAF1), cyclin-dependent kinase inhibitor 1B (p27, 10pl) (p27^WAF1), phosphatase and tensin hornolog (mutated in multiple advanced cancers 1) (PTEN), retinoic acid receptor, beta (RAR-, Ras association (RaIGDS/AF-6) domain family 1 C (RASSFIC), secreted frizzled-related protein 1 (SFRP1), tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy, pseudoinfiammatory) (TIMP3),and von HippeI-Lindau syndrome (VHL). The rest 17 targets exhibited to various extents the tumor associated changes.As changes in methylation of the following genes occurred marginally, their impact on the formation of colorectal cancer were trivial: adenomatous polyposis coli (APC) (8%, 5165),Ras association (RaIGDS/AF-6) domain family 1A (RASSFIA) (3%, 2/65) and cyclin-dependent kinase inhibitor 2A,alternated reading frame Co14~) (6%, 4/65). The following genes exhibited moderate changes in rnethylation: O-6rnethylguanine-DNA rnethyltransferase (MGMT) (20%, 13/65),rnutL hornolog 1, colon cancer, nonpolyposis type 2 (E. coli) (hMLH1) (18%, 12/65), cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) P16^NK4a) (10%, 10/65),rnethylated in tumor 1 (MINT1) (15%, 10/65), methylated in tumor 31 (MINT31) (11%, 7/65). The rest changed greatly in the rnethylation pattern in colorectal cancer (CRC): cyclin A1 (cyclin al) (100%, 65/65), caudal type homeobox transcriptdon factor 1 (CDX1) (100%, 65/65), RAR(85%, 55/65), myogenic factor 3 (MYOD1) (69%, 45/65),cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)(p15^INK4b) (68%, 44/65), prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (COX2) (72%, 47/65), cadherin 13, H-cadherin (heart) (CDH13) (65%, 42/65), CAAX box 1 (OO~/) (58%, 38/65),tumor protein p73 (p73) (63%, 41/65) and Wilrns tumor 1 (WT/) (58%, 38/65). However, no significant correlation of changes in rnethylation with any given clinical-pathological features was detected. Furthermore, the frequent changes in rnethylation appeared to be an early phase event of colon carcinogenesis. The in situ expression of 10 genes was assessed by the irnrnunohistochernical approach at the protein level: CDH1, CDH13, COX2, cyclin A1, hMLH1,MGMT, p14^ARF, p73, RAR-, and TIMP3 genes in the context of the rnethylation status in colorectal cancer. No clear correlation between the hyperrnethylation of the promoter CpG islands and the negative expression of the genes was established.CONCLUSION: The methylation profile of 31 genes was established in patients with colon cancer and colorectal adenornas, which provides new insights into the DNA rnethylation mediated mechanisms underlying the carcinogenesis of colorectal cancer and may be of prognostic values for colorectal cancer.
基金The National Basic Research Program(973)of China, No.G1998051203
文摘AIM: To observe the expression of cyclooxygenase-2 (COX-2) and to investigate the association between COX-2expression and infection with cytotoxic-associated gene A( cagA) positive strair Helicobacter pylori ( Hp) in humangastric cancer, and subsequently to provide fresh ideas forthe early prevention of gastric cancer.METHODS: 32 Specimens of gastric cancer andcorresponding adjacent normal gastric mucosa were obtainedfrom patients who had undergone surgical operations ofgastric cancer. All the samples including 1 case of stomachmalignant lymphoma and 31 cases of gastric adenocarcinomawere confirmed by pathology diagnosis. The expression ofCOX-2 in 32 specimens of gastric cancer and correspondingadjacent normal gastric mucosa was quantitativelydetermined and analyzed with Flow Cytometry, and the levelsof COX-2 protein were compared between specimens withcagA+ Hp infection and those without cagA+ Hp infection.The cagA gene in 32 specimens of gastric cancer wasdetected bypolymerase chain reaction (PCR) method.RESULTS: Twenty-seven of 32 (84 %) specimens of gastriccancer showed over-expression of COX-2, compared withthe adjacent normal gastric mucosa. cagA+ gene weredetected from 19 specimens of gastric cancer, but not fromthe other 13 specimens. The levels of COX-2 protein in 19specimens of gastric cancer with cagA+ Hp infection (thenumber of positive cells was 73.82±18.2) were significantlyhigher than those in the 13 specimens without cagA+ Hpinfection (the number of positive cells was 35.92±22.1).CONCLUSION: COX-2 is overexpressed in gastric cancerand cagA+Hp infection could up-regulate the expression ofCOX-2 in gastric cancer in human. There may also existanother way or channel to regulate the expression of COX-2 in gastric cancer in addition to cagA+Hp infection.Therefore, applying COX-2 selective inhibitors could be aneffective and promising way to prevent gastric cancer.
基金supported by Grants from the National Natural Science Foundation of China (Nos. 21171086 and 81160213)Inner Mongolia Grassland Talent (No. 108-108038)+2 种基金Natural Science Foundation of Inner Mongolia Autonomous Region of China (Nos. 2013MS1121 and 2015MS0806)Inner Mongolia Department of Science and Technology (No. 211-202077)the Inner Mongolia Agricultural University (Nos. 109-108040, 211-109003, and 211-206038)
文摘Mercury ions have been considered highly toxic to human health. What would be great is to develop the ionic probes without any toxicities themselves. Here, we report a friendly, highly sensitive mercury(II) ionic probe, watersoluble photoluminescence carbon dots which were synthesized by simply hydrothermal treatment of fresh cherry tomatoes without adding any other reagents. The ultra-small(\1 nm) carbon dots show robust excitation-depended photoluminescence under a wide p H range(4–10) or a strong ionic strength of up to 1 M, and the detection limit of mercury(II) has been determined as low as 18 n M. We envision such water-soluble, biocompatible carbon dots that could be applied to biolabeling, bio-imaging, and biosensing fields.
基金Supported by the National Natural Science Foundation of China(21736008 and U1662126).
文摘The inlet temperature of the Vacuum Gas Oil(VGO)hydrotreating reactor of a refinery is analyzed with the integration of multiple series reactors and hydrogen network considered.The effect of the inlet temperature(T1)on hydrogen sinks/sources and the product output is analyzed systematically based on the simulation of the series reactors,including VGO hydrotreating reactor,hydrocracking reactor,fluid catalytic cracking reactor and visbreaking reactor.The general relation between the Hydrogen Utility Adjustment(HUA)and multiple pairs of varying sinks and sources is deduced,and correlations between varying streams and T1 are linearly fitted.Based on this,the quantitative equation between HUA and T1 is derived,and corresponding diagram is constructed.The T1 corresponding the minimum hydrogen consumption is identified to be 345°C.
文摘OBJECTIVE To analyze our management strategy and results of treating patients affected by meningiomas invading the sagittal or transverse sinuses.METHOOS Review of data from 35 patients with pathologically confirmed meningiomas(29 of the sagittal sinus and 6 of the transverse sinus)surgically treated between from July 1999 and June 2003,including clinical manifestations,mode of diagnosis and curative effect of microsurgery etc.For our surgical decision-making,meningiomas were classified into six types based on the degree of sinus involvement.RESULTS A Simpson's GradeⅠresection was achieved in 27 cases(77.1%),GradeⅡin 6(17.1%)and GradeⅢin 2(5.7%).No patients died after the operations.The recurrence rate in the study overall was 2.9%,with a follow-up period from 3 to 6 years.CONCLUSION Application of microsurgical techniques,protection of the sinus,avoidance of damages to the cerebral cortex,veins of the central sulcus,as well as other veins from the tumor,are the major factors for increasing the rate of total resection,reducing complications and improving the quality of life for the patients with meningiomas invading the sagittal or transverse sinuses.
文摘The paper introduces a new class of numerical schemes for the approximate solutions of stochastic pantograph equations. As an effective technique to implement implicit stochastic methods, strong predictor-corrector methods （PCMs） are designed to handle scenario simulation of solutions of stochastic pantograph equations. It is proved that the PCMs are strong convergent with order 1/2.Linear M^-stabiiity of stochastic pantograph equationsand the PCMs are researched in the paper. Sufficient conditions of MS-unstability of stochastic pantograph equations and MS-stability of the PCMs are obtained, respectively. Numerical experiments demonstrate these theoretical results.
文摘In this paper the homotopy continuation method for stochastic two-point boundary value problems driven by additive noises is studied. The existence of the solution of the homotopy equation is proved. Numerical schemes are constructed and error estimates are obtained. Numerical experiments demonstrate the effectiveness of the homotopy continu- ation method over other commonly used methods such as the shooting method.
基金The authors are very grateful to the anonymous referees for their valuable comments and suggestions. We want to thank Mr. Liang Chen at Hunan University for many useful comments. This work was supported by the National Natural Science Foundation of China under Grant 11271117.
文摘This paper aims to investigate sufficient conditions for the recovery of sparse signals via the orthogonal matching pursuit （OMP） algorithm. In the noiseless case, we present a novel sufficient condition for the exact recovery of all k-sparse signals by the OMP algorithm, and demonstrate that this condition is sharp. In the noisy case, a sufficient condition for recovering the support of k-sparse signal is also presented. Generally, the computation for the restricted isometry constant （RIC） in these sufficient conditions is typically difficult, therefore we provide a new condition which is not only computable but also sufficient for the exact recovery of all k-sparse signals.
文摘The median follow-up of the malignant glioma patients who received surgical resection combined with radiochemotherapy is 16 months. The recurrence of malignant glioma is unavoidable. Although the patient with recurrent glioblastoma has multiple choices of therapy, the 5-year survival rate is still less than 5%, and the average survival time is less than 12 months. Currently, there is no single second-line drug can have survival benefits.
