Border-associated macrophages are located at the interface between the brain and the periphery, including the perivascular spaces, choroid plexus, and meninges. Until recently, the functions of border-associated macro...Border-associated macrophages are located at the interface between the brain and the periphery, including the perivascular spaces, choroid plexus, and meninges. Until recently, the functions of border-associated macrophages have been poorly understood and largely overlooked. However, a recent study reported that border-associated macrophages participate in stroke-induced inflammation, although many details and the underlying mechanisms remain unclear. In this study, we performed a comprehensive single-cell analysis of mouse border-associated macrophages using sequencing data obtained from the Gene Expression Omnibus(GEO) database(GSE174574 and GSE225948). Differentially expressed genes were identified, and enrichment analysis was performed to identify the transcription profile of border-associated macrophages. CellChat analysis was conducted to determine the cell communication network of border-associated macrophages. Transcription factors were predicted using the ‘pySCENIC' tool. We found that, in response to hypoxia, borderassociated macrophages underwent dynamic transcriptional changes and participated in the regulation of inflammatory-related pathways. Notably, the tumor necrosis factor pathway was activated by border-associated macrophages following ischemic stroke. The pySCENIC analysis indicated that the activity of signal transducer and activator of transcription 3(Stat3) was obviously upregulated in stroke, suggesting that Stat3 inhibition may be a promising strategy for treating border-associated macrophages-induced neuroinflammation. Finally, we constructed an animal model to investigate the effects of border-associated macrophages depletion following a stroke. Treatment with liposomes containing clodronate significantly reduced infarct volume in the animals and improved neurological scores compared with untreated animals. Taken together, our results demonstrate comprehensive changes in border-associated macrophages following a stroke, providing a theoretical basis for targeting border-associated macrophages-induced neuroinflammation in stroke treatment.展开更多
Substrate and nutrient supply are essential for vegetable cultivation in greenhouse.The strategies for plant nutrient supply vary depending on the cultivation methods or substrate dosages employed.With the development...Substrate and nutrient supply are essential for vegetable cultivation in greenhouse.The strategies for plant nutrient supply vary depending on the cultivation methods or substrate dosages employed.With the development of mechanization,wide-row spacing substrate cultivation became an optimize mode of the greenhouse cucumber cultivation,aligning with the trend of intelligent agriculture.To determine the optimal nutrient solution supply amount(NS)and supply frequency(SF)for promoting the integrated growth of cucumber under wide-row spacing substrate cultivation,we explored the effects of substrate supply amount(SS),NS,and SF on cucumber yield,quality,and element utilization efficiency.A five-level quadratic orthogonal rotation combination design with three experimental factors(NS,SF,and SS)was implemented for 23 coupling treatments over three growing seasons,including spring(2022S and 2023S)and autumn(2022A).The technique for order preference by similarity to ideal solution(TOPSIS)combining weights based on game theory was applied to construct cucumber comprehensive growth evaluation model.Single and two experimental factors analyses revealed significant effects of single factors and the coupling of NS-SS,NS-SF and SS-SF on the integrated growth of cucumber for all three growing seasons.For the NS-SF-SS combination,the optimal parameters for comprehensive cucumber growth were determined as follows:levels of^(-1).68 for NS,-0.7 for SF,and^(-1).682 for SS in 2022A;-0.43 for NS,-0.06 for SF,and 0.34 for SS in 2022S;0.3 for NS,-0.02 for SF,and 0.04 for SS in 2023S.Furthermore,for SS ranges of 2.00-3.01,3.01-4.50,4.50-5.99,5.99-7.00(L·plant^(-1)),the corresponding NS and SF intervals maximizing cucumber integrated growth in spring were:0.28-0.30(L·plant^(-1))and 6(times·d^(-1)),0.26-0.30(L·plant^(-1))and 6(times·d^(-1)),0.25-0.30(L·plant^(-1))and 6(times·d^(-1)),0.23-0.30(L·plant^(-1))and 6(times·d^(-1)),respectively.With the same SS,the corresponding NS and SF intervals that maximized cucumber integrated growth in autumn were:0.10(L·plant^(-1))and 8(times·d^(-1)),0.18(L·plant^(-1))and 7(times·d^(-1)),0.30(L·plant^(-1))and 6(times·d^(-1)),0.49(L·plant^(-1))and 5(times·d^(-1)),respectively.The results provide a theoretical basis for solution management,and further in-depth research on cucumber cultivation.展开更多
Accurate estimation of battery health status plays a crucial role in battery management systems.However,the lack of operational data still affects the accuracy of battery state of health(SOH)estimation.For this reason...Accurate estimation of battery health status plays a crucial role in battery management systems.However,the lack of operational data still affects the accuracy of battery state of health(SOH)estimation.For this reason,a SOH estimation method is proposed based on charging data reconstruction combined with image processing.The charging voltage data is used to train the least squares generative adversarial network(LSGAN),which is validated under different levels of missing data.From a visual perspective,the Gram angle field method is applied to convert one-dimensional time series data into image data.This method fully preserves the time series characteristics and nonlinear evolution patterns,which avoids the difficulties and limited expressive power associated with manual feature extraction.At the same time,the Swin Transformer model is introduced to extract global structures and local details from images,enabling better capture of sequence change trends.Combined with the long short-term memory network(LSTM),this enables accurate estimation of battery SOH.Two different types of batteries are used to validate the test.The experimental results show that the proposed method has good estimation accuracy under different training proportions.展开更多
The blood-brain barrier(BBB)is a major challenge in drug delivery for the treatment of central nervous system diseases.Walnut derived peptide TWLPLPR(TW-7)has been proved to promote neuronal mitochondrial autophagy an...The blood-brain barrier(BBB)is a major challenge in drug delivery for the treatment of central nervous system diseases.Walnut derived peptide TWLPLPR(TW-7)has been proved to promote neuronal mitochondrial autophagy and enhance hippocampal neuronal synaptic plasticity,thereby improving learning and memory abilities in mice.We investigated the internalization mechanism and intracellular transport pathway for the walnut-derived peptide,TW-7,using b End.3 cells in an in vitro BBB model system.TW-7 was taken up by the b End.3 cells in a concentration-,temperature-,and energy-dependent manner;this involved increases in caveolin-1 and caveolin-2 protein expression and phosphorylation and inhibition of P-glycoprotein-mediated efflux.Subcellular localization of TW-7 in b End.3 cells was observed,indicating that the plasma membrane,endoplasmic reticulum,Golgi apparatus,lysosomes,and mitochondria participated in intracellular trafficking and that the peptide escaped from lysosomes over time.Caveolae may be critical for TW-7 uptake by brain microvascular endothelial cells,assisting TW-7 to cross the BBB.The results of this study provide a theoretical basis for the mechanism of active peptide penetrating the BBB,and provide a reference for developing neuroprotective active peptide products.展开更多
Herein,we demonstrated the integration of BiVO_(4)-based photoanode with metallic Zn cathode for high-performance CO_(2)reduction,and a record CO production rate of 113.32μmol cm^(−2)h^(−1)with a FECO of 90.57%has be...Herein,we demonstrated the integration of BiVO_(4)-based photoanode with metallic Zn cathode for high-performance CO_(2)reduction,and a record CO production rate of 113.32μmol cm^(−2)h^(−1)with a FECO of 90.57%has been achieved under simulated sunlight(AM 1.5 G,100 mW),accompanying with an excellent stability.More importantly,the direct observation of spatial charge separation/transfer and dynamic surface catalysis for both H_(2)O oxidation and CO_(2)reduction has been firstly achieved by the combination of in situ X-ray photoelectron spectroscopy(IS-XPS)with Fourier transform infrared reflection(IS-FTIR).Under light irradiation,the electron-hole pairs have been generated on BiVO_(4) photoanode,and holes rapidly transfer to photoanode surfaces for participating in oxygen evolution reaction(OER)through the formation of*OH and*OOH intermediates.Simultaneously,the proton-coupled electron transfer to the Zn cathode surfaces drive the reduction of adsorbed CO_(2)molecules into CO via the formation*COOH and*CO intermediates.Thereby,this work offers new insights into fundamental understanding of CO_(2)reduction process,which facilitates the future development of highly efficient carbon fixation systems.展开更多
Catalpa bungei,a fast-growing timber tree,is threatened by the lepidopteran pest Omphisa plagialis.Previous studies in our laboratory successfully generated transgenic C.bungei lines overexpressing Cry genes(Cry1Ab,Cr...Catalpa bungei,a fast-growing timber tree,is threatened by the lepidopteran pest Omphisa plagialis.Previous studies in our laboratory successfully generated transgenic C.bungei lines overexpressing Cry genes(Cry1Ab,Cry2A,and Cry9-2)that exhibited resistance to O.plagialis,but their potential impact on soil bacterial communities remains unclear.In this study,we analyzed nine transgenic C.bungei lines(three independent lines for each Cry gene)to characterize their rhizosphere bacterial communities using high-throughput sequencing of the 16S ribosomal DNA(rDNA)V4-V5 regions.A total of 628 amplicon sequence variants(ASVs)were shared among all transgenic and wild-type(WT)lines,forming a stable core microbiome dominated by Proteobacteria,Bacteroidota,Acidobacteriota,and Actinobacteriota.Alpha diversity showed no significant differences,while beta diversity revealed minor but distinct compositional shifts.Cry1Ab lines exhibited higher abundances of fast-growing taxa,particularly Proteobacteria and Bacteroidota;Cry2A lines displayed intermediate profiles,whereas Cry9-2 lines were nearly indistinguishable from WT communities.Linear discriminant analysis of the effect size revealed significant enrichment of taxa such as Burkholderiaceae and Ralstonia in the Cry1Ab rhizosphere,in contrast to the higher abundance of Chloroflexi in the WT.Functional predictions indicated consistent metabolic pathways across all treatments,suggesting strong ecological redundancy.This study demonstrates minimal impact on rhizosphere microbial communities in transgenic C.bungei plants.The Cry9-2 construct exhibited superior environmental stability,whereas the Cry1Ab construct caused only slight but ecologically acceptable shifts.These findings support the ecological safety of Bt-transgenic C.bungei and identify Cry9-2 as a particularly favorable candidate for forestry applications.This comparative evaluation of three Cry genes in a tree species provides a framework for future gene-specific biosafety assessments in woody plants.展开更多
The unique crystallographic lamellar microstructure(CLM) Ni-based superalloys fabricated by laser powder bed fusion(LPBF) exhibits excellent tensile properties.This study aims to investigate CLM's high-temperature...The unique crystallographic lamellar microstructure(CLM) Ni-based superalloys fabricated by laser powder bed fusion(LPBF) exhibits excellent tensile properties.This study aims to investigate CLM's high-temperature stress rupture behavior and use these findings to improve the additive manufacturing process.The result shows that the high temperature-induced intergranular fracture in <110> grain region is responsible for stress rupture failure under both conditions of 760 ℃/780 MPa and 980 ℃/260 MPa.Among them,the sub-grain boundary fracture occurs only under high temperature and low stress,980 ℃/260 MPa.Due to the severe intergranular fracture induced by stray grains,the stress rupture life is very low under both conditions.According to the finite element simulation,the formation of stray grains stems from the unstable heat flow within the melt pool during the process.In addition,the shorter stress rupture lifetime does not excite a more pronounced dislocation network around the γ′ phase.However,the deformation twins can still be activated inside the <110> grains,so it has excellent plasticity under both test conditions.Finally,this work indicates that the future optimization of CLM by LPBF should focus on eliminating of high-angle grain boundaries in <110> grains.展开更多
Necrotizing fasciitis(NF)is a rapidly progressing,life-threatening soft tissue infection,with upper limb NF posing a particularly serious threat to patient survival and quality of life.Negative pressure wound therapy(...Necrotizing fasciitis(NF)is a rapidly progressing,life-threatening soft tissue infection,with upper limb NF posing a particularly serious threat to patient survival and quality of life.Negative pressure wound therapy(NPWT)has shown considerable advantages in accelerating wound healing and mitigating functional impairment.A retrospective study by Lipatov et al.demonstrated that NPWT significantly reduced the time needed for wound closure preparation while enhancing the success rate of local repair.Despite its benefits,certain limitations highlight the need for further optimization.This paper investigates the potential for personalized dynamic regulation of NPWT,its integration with adjunctive therapies,and the role of multidisciplinary collaboration.Furthermore,it explores the incorporation of advanced technologies such as artificial intelligence,imaging modalities,and biomaterials,presenting novel pathways for the personalized management and global standardization of NF treatment.展开更多
The heritage preservation is of great intractability to the conservators as each kind of heritage material has unique and diverse requirements on temperature,humidity and air cleanliness.It is promising for metal-orga...The heritage preservation is of great intractability to the conservators as each kind of heritage material has unique and diverse requirements on temperature,humidity and air cleanliness.It is promising for metal-organic frameworks(MOFs),the multifunctional environment remediation materials,to be applied in heritage environmental protection.The advantages of MOFs lie in their multifunction like adsorption,photocatalysis,sterilization,as well as the controllable structure and properties that could be flexibly adjusted as demands,helping the heritage against various environmental threats.Thereby,the applications and the corresponding mechanisms of MOFs in cultural heritage preservation were reviewed in this work,including harmful gas adsorption,surface waterproofing,particulate matters(PM)removal,anti-bacterial and humidity control of environment.Finally,the selection principles and precautions of MOFs in heritage preservation were discussed,aiming to provide a forward-looking direction for the selection and application of MOFs.展开更多
Advanced geological prediction is a crucial means to ensure safety and efficiency in tunnel construction.However,diff erent advanced geological forecasting methods have their own limitations,resulting in poor detectio...Advanced geological prediction is a crucial means to ensure safety and efficiency in tunnel construction.However,diff erent advanced geological forecasting methods have their own limitations,resulting in poor detection accuracy.Using multiple methods to carry out a comprehensive evaluation can eff ectively improve the accuracy of advanced geological prediction results.In this study,geological information is combined with the detection results of geophysical methods,including transient electromagnetic,induced polarization,and tunnel seismic prediction,to establish a comprehensive analysis method of adverse geology.First,the possible main adverse geological problems are determined according to the geological information.Subsequently,various physical parameters of the rock mass in front of the tunnel face can then be derived on the basis of multisource geophysical data.Finally,based on the analysis results of geological information,the multisource data fusion algorithm is used to determine the type,location,and scale of adverse geology.The advanced geological prediction results that can provide eff ective guidance for tunnel construction can then be obtained.展开更多
基金supported by Qingdao Key Medical and Health Discipline ProjectThe Intramural Research Program of the Affiliated Hospital of Qingdao University,No. 4910Qingdao West Coast New Area Science and Technology Project,No. 2020-55 (all to SW)。
文摘Border-associated macrophages are located at the interface between the brain and the periphery, including the perivascular spaces, choroid plexus, and meninges. Until recently, the functions of border-associated macrophages have been poorly understood and largely overlooked. However, a recent study reported that border-associated macrophages participate in stroke-induced inflammation, although many details and the underlying mechanisms remain unclear. In this study, we performed a comprehensive single-cell analysis of mouse border-associated macrophages using sequencing data obtained from the Gene Expression Omnibus(GEO) database(GSE174574 and GSE225948). Differentially expressed genes were identified, and enrichment analysis was performed to identify the transcription profile of border-associated macrophages. CellChat analysis was conducted to determine the cell communication network of border-associated macrophages. Transcription factors were predicted using the ‘pySCENIC' tool. We found that, in response to hypoxia, borderassociated macrophages underwent dynamic transcriptional changes and participated in the regulation of inflammatory-related pathways. Notably, the tumor necrosis factor pathway was activated by border-associated macrophages following ischemic stroke. The pySCENIC analysis indicated that the activity of signal transducer and activator of transcription 3(Stat3) was obviously upregulated in stroke, suggesting that Stat3 inhibition may be a promising strategy for treating border-associated macrophages-induced neuroinflammation. Finally, we constructed an animal model to investigate the effects of border-associated macrophages depletion following a stroke. Treatment with liposomes containing clodronate significantly reduced infarct volume in the animals and improved neurological scores compared with untreated animals. Taken together, our results demonstrate comprehensive changes in border-associated macrophages following a stroke, providing a theoretical basis for targeting border-associated macrophages-induced neuroinflammation in stroke treatment.
基金supported by the China Agriculture Research System(Grant No.CARS-23-D06)the Key Research and Development Program of Shaanxi Province(Grant Nos.2024NC2-GJHX-29 and 2024NC-ZDCYL-05-08)Shaanxi Agricultural Collaborative Innovation and Extension Alliance Project(Grant No.LMZD202202).
文摘Substrate and nutrient supply are essential for vegetable cultivation in greenhouse.The strategies for plant nutrient supply vary depending on the cultivation methods or substrate dosages employed.With the development of mechanization,wide-row spacing substrate cultivation became an optimize mode of the greenhouse cucumber cultivation,aligning with the trend of intelligent agriculture.To determine the optimal nutrient solution supply amount(NS)and supply frequency(SF)for promoting the integrated growth of cucumber under wide-row spacing substrate cultivation,we explored the effects of substrate supply amount(SS),NS,and SF on cucumber yield,quality,and element utilization efficiency.A five-level quadratic orthogonal rotation combination design with three experimental factors(NS,SF,and SS)was implemented for 23 coupling treatments over three growing seasons,including spring(2022S and 2023S)and autumn(2022A).The technique for order preference by similarity to ideal solution(TOPSIS)combining weights based on game theory was applied to construct cucumber comprehensive growth evaluation model.Single and two experimental factors analyses revealed significant effects of single factors and the coupling of NS-SS,NS-SF and SS-SF on the integrated growth of cucumber for all three growing seasons.For the NS-SF-SS combination,the optimal parameters for comprehensive cucumber growth were determined as follows:levels of^(-1).68 for NS,-0.7 for SF,and^(-1).682 for SS in 2022A;-0.43 for NS,-0.06 for SF,and 0.34 for SS in 2022S;0.3 for NS,-0.02 for SF,and 0.04 for SS in 2023S.Furthermore,for SS ranges of 2.00-3.01,3.01-4.50,4.50-5.99,5.99-7.00(L·plant^(-1)),the corresponding NS and SF intervals maximizing cucumber integrated growth in spring were:0.28-0.30(L·plant^(-1))and 6(times·d^(-1)),0.26-0.30(L·plant^(-1))and 6(times·d^(-1)),0.25-0.30(L·plant^(-1))and 6(times·d^(-1)),0.23-0.30(L·plant^(-1))and 6(times·d^(-1)),respectively.With the same SS,the corresponding NS and SF intervals that maximized cucumber integrated growth in autumn were:0.10(L·plant^(-1))and 8(times·d^(-1)),0.18(L·plant^(-1))and 7(times·d^(-1)),0.30(L·plant^(-1))and 6(times·d^(-1)),0.49(L·plant^(-1))and 5(times·d^(-1)),respectively.The results provide a theoretical basis for solution management,and further in-depth research on cucumber cultivation.
基金supported in part by the National Natural Science Foundation of China(under Grant 62473309,62203352)the Shaanxi Outstanding Youth Science Fund Project(under Grant 2024JC-JCQN-68)+1 种基金the Xi’an Science and Technology Plan Project(under Grant 24GXFW0050)the Xi’an Key Laboratory(under Grant 24ZDSY0015).
文摘Accurate estimation of battery health status plays a crucial role in battery management systems.However,the lack of operational data still affects the accuracy of battery state of health(SOH)estimation.For this reason,a SOH estimation method is proposed based on charging data reconstruction combined with image processing.The charging voltage data is used to train the least squares generative adversarial network(LSGAN),which is validated under different levels of missing data.From a visual perspective,the Gram angle field method is applied to convert one-dimensional time series data into image data.This method fully preserves the time series characteristics and nonlinear evolution patterns,which avoids the difficulties and limited expressive power associated with manual feature extraction.At the same time,the Swin Transformer model is introduced to extract global structures and local details from images,enabling better capture of sequence change trends.Combined with the long short-term memory network(LSTM),this enables accurate estimation of battery SOH.Two different types of batteries are used to validate the test.The experimental results show that the proposed method has good estimation accuracy under different training proportions.
基金supported by the National Natural Science Foundation of China(22378368).
文摘The blood-brain barrier(BBB)is a major challenge in drug delivery for the treatment of central nervous system diseases.Walnut derived peptide TWLPLPR(TW-7)has been proved to promote neuronal mitochondrial autophagy and enhance hippocampal neuronal synaptic plasticity,thereby improving learning and memory abilities in mice.We investigated the internalization mechanism and intracellular transport pathway for the walnut-derived peptide,TW-7,using b End.3 cells in an in vitro BBB model system.TW-7 was taken up by the b End.3 cells in a concentration-,temperature-,and energy-dependent manner;this involved increases in caveolin-1 and caveolin-2 protein expression and phosphorylation and inhibition of P-glycoprotein-mediated efflux.Subcellular localization of TW-7 in b End.3 cells was observed,indicating that the plasma membrane,endoplasmic reticulum,Golgi apparatus,lysosomes,and mitochondria participated in intracellular trafficking and that the peptide escaped from lysosomes over time.Caveolae may be critical for TW-7 uptake by brain microvascular endothelial cells,assisting TW-7 to cross the BBB.The results of this study provide a theoretical basis for the mechanism of active peptide penetrating the BBB,and provide a reference for developing neuroprotective active peptide products.
基金supported by the National Natural Science Foundation of China(21832005,22372181,22072168,22002175)Major Program of the Lanzhou Institute of Chemical Physics,CAS(ZYFZFX-3),Major Science and Technology Projects in Gansu Province(22ZD6GA003)the CAS“Light of West China”Program and West Light Foundation of The Chinese Academy of Sciences(xbzg-zdsys-202209).
文摘Herein,we demonstrated the integration of BiVO_(4)-based photoanode with metallic Zn cathode for high-performance CO_(2)reduction,and a record CO production rate of 113.32μmol cm^(−2)h^(−1)with a FECO of 90.57%has been achieved under simulated sunlight(AM 1.5 G,100 mW),accompanying with an excellent stability.More importantly,the direct observation of spatial charge separation/transfer and dynamic surface catalysis for both H_(2)O oxidation and CO_(2)reduction has been firstly achieved by the combination of in situ X-ray photoelectron spectroscopy(IS-XPS)with Fourier transform infrared reflection(IS-FTIR).Under light irradiation,the electron-hole pairs have been generated on BiVO_(4) photoanode,and holes rapidly transfer to photoanode surfaces for participating in oxygen evolution reaction(OER)through the formation of*OH and*OOH intermediates.Simultaneously,the proton-coupled electron transfer to the Zn cathode surfaces drive the reduction of adsorbed CO_(2)molecules into CO via the formation*COOH and*CO intermediates.Thereby,this work offers new insights into fundamental understanding of CO_(2)reduction process,which facilitates the future development of highly efficient carbon fixation systems.
基金funded by the Chinese Academy of Forestry-Special funds for basic scientific research service expenses of the central level public welfare research institutes(Grant No.CAFYBB2020QD001)the National Natural Science Foundation of China(Grant Nos.32101550,32271917)+1 种基金Jiangsu Agricultural Science and Technology Innovation Fund(Grant No.CX(24)3052)National Forestry and Grassland Administration’s Center for Science and Technology Development Projects(Grant No.KJZXSA202202).
文摘Catalpa bungei,a fast-growing timber tree,is threatened by the lepidopteran pest Omphisa plagialis.Previous studies in our laboratory successfully generated transgenic C.bungei lines overexpressing Cry genes(Cry1Ab,Cry2A,and Cry9-2)that exhibited resistance to O.plagialis,but their potential impact on soil bacterial communities remains unclear.In this study,we analyzed nine transgenic C.bungei lines(three independent lines for each Cry gene)to characterize their rhizosphere bacterial communities using high-throughput sequencing of the 16S ribosomal DNA(rDNA)V4-V5 regions.A total of 628 amplicon sequence variants(ASVs)were shared among all transgenic and wild-type(WT)lines,forming a stable core microbiome dominated by Proteobacteria,Bacteroidota,Acidobacteriota,and Actinobacteriota.Alpha diversity showed no significant differences,while beta diversity revealed minor but distinct compositional shifts.Cry1Ab lines exhibited higher abundances of fast-growing taxa,particularly Proteobacteria and Bacteroidota;Cry2A lines displayed intermediate profiles,whereas Cry9-2 lines were nearly indistinguishable from WT communities.Linear discriminant analysis of the effect size revealed significant enrichment of taxa such as Burkholderiaceae and Ralstonia in the Cry1Ab rhizosphere,in contrast to the higher abundance of Chloroflexi in the WT.Functional predictions indicated consistent metabolic pathways across all treatments,suggesting strong ecological redundancy.This study demonstrates minimal impact on rhizosphere microbial communities in transgenic C.bungei plants.The Cry9-2 construct exhibited superior environmental stability,whereas the Cry1Ab construct caused only slight but ecologically acceptable shifts.These findings support the ecological safety of Bt-transgenic C.bungei and identify Cry9-2 as a particularly favorable candidate for forestry applications.This comparative evaluation of three Cry genes in a tree species provides a framework for future gene-specific biosafety assessments in woody plants.
基金the financial support by the Project of Taihang Laboratory (No. A3023)Science Center for Gas Turbine Project (Grant No. P2022-CIV-002-001)。
文摘The unique crystallographic lamellar microstructure(CLM) Ni-based superalloys fabricated by laser powder bed fusion(LPBF) exhibits excellent tensile properties.This study aims to investigate CLM's high-temperature stress rupture behavior and use these findings to improve the additive manufacturing process.The result shows that the high temperature-induced intergranular fracture in <110> grain region is responsible for stress rupture failure under both conditions of 760 ℃/780 MPa and 980 ℃/260 MPa.Among them,the sub-grain boundary fracture occurs only under high temperature and low stress,980 ℃/260 MPa.Due to the severe intergranular fracture induced by stray grains,the stress rupture life is very low under both conditions.According to the finite element simulation,the formation of stray grains stems from the unstable heat flow within the melt pool during the process.In addition,the shorter stress rupture lifetime does not excite a more pronounced dislocation network around the γ′ phase.However,the deformation twins can still be activated inside the <110> grains,so it has excellent plasticity under both test conditions.Finally,this work indicates that the future optimization of CLM by LPBF should focus on eliminating of high-angle grain boundaries in <110> grains.
基金Supported by Henan Province Key Research and Development Program,No.231111311000Henan Provincial Science and Technology Research Project,No.232102310411+2 种基金Henan Province Medical Science and Technology Key Project,No.LHGJ20220566 and No.LHGJ20240365Henan Province Medical Education Research Project,No.WJLX2023079Zhengzhou Medical and Health Technology Innovation Guidance Program,No.2024YLZDJH022.
文摘Necrotizing fasciitis(NF)is a rapidly progressing,life-threatening soft tissue infection,with upper limb NF posing a particularly serious threat to patient survival and quality of life.Negative pressure wound therapy(NPWT)has shown considerable advantages in accelerating wound healing and mitigating functional impairment.A retrospective study by Lipatov et al.demonstrated that NPWT significantly reduced the time needed for wound closure preparation while enhancing the success rate of local repair.Despite its benefits,certain limitations highlight the need for further optimization.This paper investigates the potential for personalized dynamic regulation of NPWT,its integration with adjunctive therapies,and the role of multidisciplinary collaboration.Furthermore,it explores the incorporation of advanced technologies such as artificial intelligence,imaging modalities,and biomaterials,presenting novel pathways for the personalized management and global standardization of NF treatment.
基金supported by the National Natural Science Foundation of China(Nos.52308300,52370025,52108266).
文摘The heritage preservation is of great intractability to the conservators as each kind of heritage material has unique and diverse requirements on temperature,humidity and air cleanliness.It is promising for metal-organic frameworks(MOFs),the multifunctional environment remediation materials,to be applied in heritage environmental protection.The advantages of MOFs lie in their multifunction like adsorption,photocatalysis,sterilization,as well as the controllable structure and properties that could be flexibly adjusted as demands,helping the heritage against various environmental threats.Thereby,the applications and the corresponding mechanisms of MOFs in cultural heritage preservation were reviewed in this work,including harmful gas adsorption,surface waterproofing,particulate matters(PM)removal,anti-bacterial and humidity control of environment.Finally,the selection principles and precautions of MOFs in heritage preservation were discussed,aiming to provide a forward-looking direction for the selection and application of MOFs.
基金National Natural Science Foundation of China(grant numbers 42293351,41877239,51422904 and 51379112).
文摘Advanced geological prediction is a crucial means to ensure safety and efficiency in tunnel construction.However,diff erent advanced geological forecasting methods have their own limitations,resulting in poor detection accuracy.Using multiple methods to carry out a comprehensive evaluation can eff ectively improve the accuracy of advanced geological prediction results.In this study,geological information is combined with the detection results of geophysical methods,including transient electromagnetic,induced polarization,and tunnel seismic prediction,to establish a comprehensive analysis method of adverse geology.First,the possible main adverse geological problems are determined according to the geological information.Subsequently,various physical parameters of the rock mass in front of the tunnel face can then be derived on the basis of multisource geophysical data.Finally,based on the analysis results of geological information,the multisource data fusion algorithm is used to determine the type,location,and scale of adverse geology.The advanced geological prediction results that can provide eff ective guidance for tunnel construction can then be obtained.
