Recent advances in research on extracellular vesicles have significantly enhanced their potential as therapeutic agents for neurological diseases.Owing to their therapeutic properties and ability to cross the blood–b...Recent advances in research on extracellular vesicles have significantly enhanced their potential as therapeutic agents for neurological diseases.Owing to their therapeutic properties and ability to cross the blood–brain barrier,extracellular vesicles are recognized as promising drug delivery vehicles for various neurological conditions,including ischemic stroke,traumatic brain injury,neurodegenerative diseases,glioma,and psychosis.However,the clinical application of natural extracellular vesicles is hindered by their limited targeting ability and short clearance from the body.To address these limitations,multiple engineering strategies have been developed to enhance the targeting capabilities of extracellular vesicles,thereby enabling the delivery of therapeutic contents to specific tissues or cells.Therefore,this review aims to highlight the latest advancements in natural and targeting-engineered extracellular vesicles,exploring their applications in treating traumatic brain injury,ischemic stroke,Parkinson's disease,Alzheimer's disease,amyotrophic lateral sclerosis,glioma,and psychosis.Additionally,we summarized recent clinical trials involving extracellular vesicles and discussed the challenges and future prospects of using targeting-engineered extracellular vesicles for drug delivery in treating neurological diseases.This review offers new insights for developing highly targeted therapies in this field.展开更多
This study aimed to introduce a modified Byars staged procedure and investigate its application value in patients with severe hypospadias.We retrospectively analyzed the clinical data of patients with severe hypospadi...This study aimed to introduce a modified Byars staged procedure and investigate its application value in patients with severe hypospadias.We retrospectively analyzed the clinical data of patients with severe hypospadias admitted to the First Affiliated Hospital of Sun Yat-sen University(Guangzhou,China)between October 2012 and October 2022.In total,31 patients underwent the conventional Byars procedure(conventional group),and 45 patients underwent the modified Byars staged procedure(modified group).Our modified strategy was built upon the standard Byars procedure by incorporating glansplasty during the first stage and employing a Y-shaped flap in conjunction with a glandular tunnel for urethroplasty during the second stage.Notably,there were no statistically significant differences in the preoperative baseline characteristics,duration of surgery,amount of blood loss,or occurrence of postoperative complications,including urethral fistula,stricture and diverticulum,or penile curvature,between the conventional and modified groups.However,there was a significantly lower incidence of coronal sulcus fistula(0 vs 16.1%,P=0.02)and glans dehiscence(0 vs 12.9%,P=0.02)in the surgical group than that in the conventional group.In addition,the modified group exhibited a notably greater rate of normotopic urethral opening(100.0%vs 83.9%,P=0.01)and a higher mean score on the Hypospadias Objective Penile Evaluation(HOPE;mean±standard error of mean:8.6±0.2 vs 7.9±0.3,P=0.02)than did the conventional group.In conclusion,the modified Byars staged procedure significantly reduced the risks of glans dehiscence and coronal sulcus fistula.Consequently,it offers a promising approach for achieving favorable penile esthetics,thereby providing a reliable therapeutic option for severe hypospadias.展开更多
Electrocatalytic reduction of carbon dioxide(CO_(2))with high selectivity to generate value-added chemical products and fuels,utilizing renewable electricity sources,offers an effective approach to address the continu...Electrocatalytic reduction of carbon dioxide(CO_(2))with high selectivity to generate value-added chemical products and fuels,utilizing renewable electricity sources,offers an effective approach to address the continued increase in atmospheric CO_(2).Here,we report cerium(Ce)-doped indium oxide(In2O3)electrocatalyst generated in situ on carbon paper(Ce-In_(2)O_(3)/CP),and its application in the electrochemical reduction of CO_(2)(CO_(2)RR)to formate formation with high Faradaic efficiency(FE)(reaching 97.6%at−1.7 V vs.Ag/AgCl)and excellent stability.Experimental analysis and density functional theory(DFT)calculations indicate that doping Ce onto the(222)plane of In_(2)O_(3)induces lattice distortion,which promotes electron transfer from Ce to In while adjusting the local electronic structure of the In atoms around Ce,making them more favorable for the adsorption of*OOCH intermediates and effectively lowering the energy barrier of the rate-determining step.Furthermore,Ce doping lowers the overpotential required for formate production and suppresses the hydrogen evolution reaction(HER),effectively enhancing the selectivity of formate.展开更多
Roads inevitably have defects during use,which not only seriously affect their service life but also pose a hidden danger to traffic safety.Existing algorithms for detecting road defects are unsatisfactory in terms of...Roads inevitably have defects during use,which not only seriously affect their service life but also pose a hidden danger to traffic safety.Existing algorithms for detecting road defects are unsatisfactory in terms of accuracy and generalization,so this paper proposes an algorithm based on YOLOv11.The method embeds wavelet transform convolution(WTConv)into the backbone’s C3k2 module to enhance low-frequency feature extraction while avoiding parameter bloat.Secondly,a novel multi-scale fusion diffusion network(MFDN)architecture is designed for the neck to strengthen cross-scale feature interactions,boosting detection precision.In terms of model optimization,the traditional downsampling method is discarded,and the innovative Adown(adaptive downsampling)technique is adopted,which streamlines the parameter scales while effectively mitigating the information loss problem during downsampling.Finally,in this paper,we propose Wise-PIDIoU by combining WiseIoU and MPDIoU to minimize the negative impact of low-quality anchor frames and enhance the detection capability of the model.The experimental results indicate that the proposed algorithm achieves an average detection accuracy of 86.5%for mAP@50 on the RDD2022 dataset,which is 2%higher than the original algorithm while ensuring that the amount of computation is basically unchanged.The number of parameters is reduced by 17%,and the F1 score is improved by 3%,showing better detection performance than other algorithms when facing different types of defects.The excellent performance on embedded devices proves that the algorithm also has favorable application prospects in practical inspection.展开更多
It is convincingly demonstrated by numerous studies that the self-similarity of modern multimedia network traffic is presented by Hurst parameter (H). The specific performance is that the similar degree is higher alon...It is convincingly demonstrated by numerous studies that the self-similarity of modern multimedia network traffic is presented by Hurst parameter (H). The specific performance is that the similar degree is higher along with the increase of H when H is between 0.5 and 1. However, it is doubtable that whether the complicated process of self-similarity can be described comprehensively by the parameter H only. Therefore, another important parameter cf has been proposed based on the discrete wavelet decomposition in this paper. The significance of the parameters is provided and the performance of the self-similarity process is described better.展开更多
To ensure the efficient operation and timely maintenance of wind turbines, thereby enhancing energy security, it is critical to monitor the operational status of wind turbines and promptly identify abnormal conditions...To ensure the efficient operation and timely maintenance of wind turbines, thereby enhancing energy security, it is critical to monitor the operational status of wind turbines and promptly identify abnormal conditions. This process relies on data collected over time by turbine sensors, including measurements such as current, voltage, temperature, and vibration signals. However, in practical applications, data from normal and abnormal conditions often exhibit an imbalance in quantity, posing challenges to traditional anomaly detection methods. Additionally, sensor data inherently contains temporal information, making the effective extraction of time-dependent features another key challenge. To address these issues, this paper proposes an anomaly detection method for wind turbine operations based on an improved Auxiliary Classifier Generative Adversarial Network. The proposed approach first employs the latent features of the training samples to augment the dataset and subsequently utilizes a Long Short-Term Memory network discriminator to extract temporal features from the samples for classification. This process directly outputs the anomaly detection results for test samples. To validate the effectiveness of the proposed method, this study uses a wind turbine blade icing dataset obtained from a Supervisory Control and Data Acquisition system. The proposed method is compared with other commonly used anomaly detection approaches. The validation and comparison results demonstrate that the proposed method achieves the lowest false alarm and missed detection rates on the blade icing dataset, underscoring its superior performance in wind turbine anomaly detection.展开更多
NiTi shape memory alloy was modified by Ag ion implantation with different incident doses to improve its antibacterial property. The atomic force microscopy, auger electron spectroscopy, and X-ray photoelectron spectr...NiTi shape memory alloy was modified by Ag ion implantation with different incident doses to improve its antibacterial property. The atomic force microscopy, auger electron spectroscopy, and X-ray photoelectron spectroscopy show that the surface of NiTi alloy is covered by TiO2 nano-film with embedded pure Ag with a peak concentration of 5.0 at% at the incident dose of 1.5 x10^17 ions.cm-a, and Ni concentration is reduced in the super- ficial surface layer. The surface roughness reaches the maximum value nearly twice higher than the control sample at the incident dose of 1.5x10^17 ions.cm-2. The potentiodynamic anodic polarization curves show that the Ag-implanted NiTi samples possess higher self-corrosion potential （Ecorr） and lower self-corrosion current density （icor0 but lower breakdown potential （Ebr）. Therefore, the corrosion resistance of the Ag-NiTi is comparable to, if not better than, the untreated NiTi. The antibacterial tests reveal that there is a distinct reduction of the germ numbers on the Ag-NiTi, which is due to the direct contact between Ag and germ, and enhanced by the leaching Ag ions.展开更多
Stable carbon isotope ratio （δ13Ccarb） analysis has been widely applied to the study of the inter-conti- nental or global marine carbonate correlation. Large-scale Cambrian-Ordovician carbonate platforms were devel...Stable carbon isotope ratio （δ13Ccarb） analysis has been widely applied to the study of the inter-conti- nental or global marine carbonate correlation. Large-scale Cambrian-Ordovician carbonate platforms were developed in the Tarim Basin. But research on fluctuation character- istics and global correlation of δ13Ccarb is still weak. Based on conodont biostratigraphy and whole-rock δ13Ccarb data in the Tahe oil-gas field of the northern Tarim Basin, the global correlation and genesis of positive carbon isotope excursions in the Darriwilian--Early Katian was exam- ined. Three positive excursions were identified in the Tahe oil-gas field including the middle Darriwilian carbon iso- tope excursion （MDICE）, the Guttenberg carbon isotope excursion （GICE）, and a positive excursion within the Pygodus anserinus conodont zone which is named the Early Sandbian carbon isotope excursion （ESICE） in this paper. Furthermore, these positive excursions had no direct relation with sea level fluctuations. MDICE and GICE could be globally correlated. The Middle-Upper Ordovi- cian Saergan Formation source rocks of the Kalpin outcrops were in accordance with the geological time of MDICE and ESICE. GICE had close relationship with the source rock of the Lianglitag Formation in the basin.Massive organic carbon burial was an important factor controlling the genesis of these positive excursions.展开更多
Acellular nerve allografts can help preserve normal nerve structure and extracellular matrix composition. These allografts have low immunogenicity and are more readily available than autologous nerves for the repair o...Acellular nerve allografts can help preserve normal nerve structure and extracellular matrix composition. These allografts have low immunogenicity and are more readily available than autologous nerves for the repair of long-segment peripheral nerve defects. In this study, we repaired a 40-mm ulnar nerve defect in rhesus monkeys with tissue-engineered peripheral nerve, and compared the outcome with that of autograft. The graft was prepared using a chemical extract from adult rhesus monkeys and seeded with allogeneic Schwann cells. Pathomo- rphology, electromyogram and immunohistochemistry findings revealed the absence of palmar erosion or ulcers, and that the morphology and elasticity of the hypothenar eminence were normal 5 months postoperatively. There were no significant differences in the mean peak compound muscle action potential, the mean nerve conduction velocity, or the number of neurofilaments between the experimental and control groups. However, outcome was significantly better in the experimental group than in the blank group. These findings suggest that chemically extracted allogeneic nerve seeded with autologous Schwann cells can repair 40-mm ulnar nerve defects in the rhesus monkey. The outcomes are similar to those obtained with autologous nerve graft.展开更多
Analysis of the in situ stress orientation and magnitude in the No.4 Structure of Nanpu Sag was performed on the basis of data obtained from borehole breakout and acoustic emission measurements.On the basis of mechani...Analysis of the in situ stress orientation and magnitude in the No.4 Structure of Nanpu Sag was performed on the basis of data obtained from borehole breakout and acoustic emission measurements.On the basis of mechanical experiments,logging interpretation,and seismic data,a 3 D geological model and heterogeneous rock mechanics field of the reservoir were constructed.Finite element simulation techniques were then used for the detailed prediction of the 3 D stress field.The results indicated that the maximum horizontal stress orientation in the study area was generally NEE-SWW trending,with significant changes in the in situ stress orientation within and between fault blocks.Along surfaces and profiles,stress magnitudes were discrete and the in situ stress belonged to theⅠa-type.Observed inter-strata differences were characterized as five different types of in situ stress profile.Faults were the most important factor causing large distributional differences in the stress field of reservoirs within the complex fault blocks.The next important influence on the stress field was the reservoir’s rock mechanics parameters,which impacted on the magnitudes of in situ stress magnitudes.This technique provided a theoretical basis for more efficient exploration and development of low-permeability reservoirs within complex fault blocks.展开更多
Many therapies are effective in treating varicoceles,including dilation of the pampiniform plexus in males.The most common method of treatment is varicocelectomy.We aimed to assess an alternative technique(microsurgic...Many therapies are effective in treating varicoceles,including dilation of the pampiniform plexus in males.The most common method of treatment is varicocelectomy.We aimed to assess an alternative technique(microsurgical spermatic[distal end]-superficial or inferior epigastric vein anastomosis)that preserves the normal blood flow pattern for varicocele treatment.We retrospectively analyzed 27 men with varicocele between October 2019 and July 2020.All patients underwent microsurgical spermatic(distal end)-superficial or inferior epigastric vein anastomosis.The prognosis was reviewed retrospectively with an additional survey conducted 3 months after surgery.The mean±standard deviation of the age was 26.1±7.3 years in patients with microsurgical spermatic(distal end)-superficial or inferior epigastric vein anastomosis.The maximum diameter of the varicocele vein,perineal pain score,sperm density,and forward movement of sperm improved over 3 months after surgery.Microsurgical spermatic(distal end)-superficial or inferior epigastric vein anastomosis is a safe and efficient surgical treatment for varicoceles.展开更多
Repairing glans dehiscence after failed hypospadias repair is challenging for pediatric surgeons.Here,we introduced and evaluated a newly modified Mathieu technique,Mathieu combined tunnel(MCT),which involves multiple...Repairing glans dehiscence after failed hypospadias repair is challenging for pediatric surgeons.Here,we introduced and evaluated a newly modified Mathieu technique,Mathieu combined tunnel(MCT),which involves multiple custom-designed flaps for the shortage of flap source material after repeated operations;we also constructed a tunnel to avoid the glans incision that may carry new risks of dehiscence.This retrospective study included 26 patients who were consecutively admitted to the First Affiliated Hospital of Sun Yat-Sen University(Guangzhou,China)for glans dehiscence repair after failed hypospadias repair from October 2014 to October 2020;sixteen patients underwent surgery using the MCT(MCT group)and ten patients underwent surgery using the tubularized incised plate(TIP)technique(TIP group).The operative time,blood loss,postoperative complications,normal urethral meatus rate,success rate,and Hypospadias Objective Penile Evaluation(HOPE)score were compared between the two groups.The MCT group achieved an overall satisfactory penile appearance and voiding function,with a higher rate of normal urethral meatus(15/16,93.8%)and a lower rate of glans dehiscence(1/16,6.2%),compared with the TIP group(70.0%and 30.0%,respectively).However,these differences were not statistically significant,possibly because of the limited number of patients(all P>0.05).Mean postoperative HOPE scores were similar in the MCT group(mean±standard deviation:8.83±0.89)and TIP group(8.94±0.57)(P>0.05).No significant differences were found between the two groups in terms of blood loss and success rate,nor in the rates of various complications(e.g.,fistula,urethral stricture,and glans dehiscence).In conclusion,the MCT technique appears to be feasible and reliable for repairing glans dehiscence after failed hypospadias repair.展开更多
The three-dimensional（3D） visualization of the functional bundles in the peripheral nerve provides direct and detailed intraneural spatial information. It is useful for selecting suitable surgical methods to repair ...The three-dimensional（3D） visualization of the functional bundles in the peripheral nerve provides direct and detailed intraneural spatial information. It is useful for selecting suitable surgical methods to repair nerve defects and in optimizing the construction of tissue-engineered nerve grafts. However, there remain major technical hurdles in obtaining, registering and interpreting 2D images, as well as in establishing 3D models. Moreover, the 3D models are plagued by poor accuracy and lack of detail and cannot completely reflect the stereoscopic microstructure inside the nerve. To explore and help resolve these key technical problems of 3D reconstruction, in the present study, we designed a novel method based on re-imaging techniques and computer image layer processing technology. A 20-cm ulnar nerve segment from the upper arm of a fresh adult cadaver was used for acetylcholinesterase（ACh E） staining. Then, 2D panoramic images were obtained before and after ACh E staining under the stereomicroscope. Using layer processing techniques in Photoshop, a space transformation method was used to fulfill automatic registration. The contours were outlined, and the 3D rendering of functional fascicular groups in the long-segment ulnar nerve was performed with Amira 4.1 software. The re-imaging technique based on layer processing in Photoshop produced an image that was detailed and accurate. The merging of images was accurate, and the whole procedure was simple and fast. The least square support vector machine was accurate, with an error rate of only 8.25%. The 3D reconstruction directly revealed changes in the fusion of different nerve functional fascicular groups. In conclusion. The technique is fast with satisfactory visual reconstruction.展开更多
Spermatogenic damage may occur after vasectomy, and the damage is pressure mediated, occurring when the occluded reproductive tract is unable to accommodate additional sperm produced by the testis. This study aimed to...Spermatogenic damage may occur after vasectomy, and the damage is pressure mediated, occurring when the occluded reproductive tract is unable to accommodate additional sperm produced by the testis. This study aimed to determine the long-term effect of vasectomy on spermatogenesis in humans and clarify how the balance between sperm production in the testis and sperm storage in or removal from the tract might be maintained. During inguinal hernia repair, an open biopsy was performed to obtain testicular tissue blocks from 51 Chinese men （aged ≥ 50 years）, of whom 25 （control group） had not undergone vasectomy and 26 （vasectomized group） had undergone bilateral vasectomy 22-42 years before. Methacrylate resin-embedded testicular sections were made, and morphometric studies were performed using light microscopy. In addition, sizes of the testis and epididymis were estimated with ultrasonography. The testicular tissue blocks obtained from one control and seven vasectomized men consisted almost completely of connective tissue. In the other 43 men, significant differences were not found between the two groups in the testicular or epididymal size, qualitative histology or quantitative parameters including the mean diameter or volume fraction of the seminiferous tubules. In conclusion, sperm production and sperm storage/removal reached a static equilibrium after vasectomy, likely due to spermatogenic degeneration or less sperm production as a result of aging or due to vasectomy-induced testicular （interstitial） fibrosis. Thus, complications that might occur in association with overproduction of sperm and distension of the tract would disappear or be relieved with time.展开更多
In this investigation, Pt–Ba–Ce/c-Al2O3 catalysts were prepared by incipient wetness impregnation and experiments were performed to evaluate the influence of H2 on the evolution mechanism of nitrogen oxides (NOx) st...In this investigation, Pt–Ba–Ce/c-Al2O3 catalysts were prepared by incipient wetness impregnation and experiments were performed to evaluate the influence of H2 on the evolution mechanism of nitrogen oxides (NOx) storage and reduction (NSR). The physical and chemical properties of the Pt–Ba–Ce/c- Al2O3 catalysts were studied using a combination of characterization techniques, which showed that PtOx, CeO2, and BaCO3, whose peaks were observed in X-ray diffraction (XRD) spectra, dispersed well on the c-Al2O3, as shown by transmission electron microscope (TEM), and that the difference between Ce3+ and Ce4+, as detected by X-ray photoelectron spectroscopy (XPS), facilitated the migration of active oxygen over the catalyst. In the process of a complete NSR experiment, the NOx storage capability was greatly enhanced in the temperature range of 250–350℃, and reached a maximum value of 315.3μmol·gcat^-1 at 350℃, which was ascribed to the increase in NO2 yield. In a lean and rich cycling experiment, the results showed that NOx storage efficiency and conversion were increased when the time of H2 exposure (i.e., 30, 45, and 60 s) was extended. The maximum NOx conversion of the catalyst reached 83.5% when the duration of the lean and rich phases was 240 and 60 s, respectively. The results revealed that increasing the content of H2 by an appropriate amount was favorable to the NSR mechanism due to increased decomposition of nitrate or nitrite, and the refreshing of trapping sites for the next cycle of NSR.展开更多
The Ti-49.8at%Ni alloy was modified by Ti ion implantation to improve its corrosion resistance and biocompatibility. The chemical composition and morphologies of the Ti Ni alloy surface were determined using atomic fo...The Ti-49.8at%Ni alloy was modified by Ti ion implantation to improve its corrosion resistance and biocompatibility. The chemical composition and morphologies of the Ti Ni alloy surface were determined using atomic force microscopy(AFM), auger electron spectroscopy(AES), and X-ray photoelectron spectroscopy(XPS). The results revealed that Ti ion implantation caused the reduction of Ni concentration and the formation of a Ti O2 nano-film on the Ti Ni alloy. The phase transformation temperatures of the Ti–Ti Ni alloy remained almost invariable after Ti ion implantation. Electrochemical tests indicated that the corrosion resistance of Ti Ni increased after Ti ion implantation. Moreover, the Ni ion release rate in 0.9% Na Cl solution for the Ti Ni alloy remarkably decreased due to the barrier effect of the Ti O2 nano-film. The cell proliferation behavior on Ti-implanted Ti Ni was better than that on the untreated Ti Ni after cell culture for 1 d and 3 d.展开更多
Acute hypobaric hypoxic brain damage is a potentially fatal high-altitude sickness.Autophagy plays a critical role in ischemic brain injury,but its role in hypo-baric hypoxia(HH)remains unknown.Here we used an HH cham...Acute hypobaric hypoxic brain damage is a potentially fatal high-altitude sickness.Autophagy plays a critical role in ischemic brain injury,but its role in hypo-baric hypoxia(HH)remains unknown.Here we used an HH chamber to demonstrate that acute HH exposure impairs autophagic activity in both the early and late stages of the mouse brain,and is partially responsible for HH-induced oxidative stress,neuronal loss,and brain damage.The autophagic agonist rapamycin only promotes the initiation of autophagy.By proteome analysis,a screen showed that protein dynamin2(DNM2)potentially regulates autophagic flux.Overexpression of DNM2 significantly increased the formation of autolysosomes,thus maintaining autophagic flux in combination with rapamycin.Furthermore,the enhancement of autophagic activity attenuated oxidative stress and neurological deficits after HH exposure.These results contribute to evidence supporting the conclusion that DNM2-mediated autophagic flux represents a new therapeutic target in HH-induced brain damage.展开更多
Multi-response metal cluster supercrystal materials,which can simultaneously display various such as color,photoluminescence,changes by bearing only one stimulus,have huge potential as stimuliresponsive intelligent ma...Multi-response metal cluster supercrystal materials,which can simultaneously display various such as color,photoluminescence,changes by bearing only one stimulus,have huge potential as stimuliresponsive intelligent material,but are rarely reported.Here,we report three Cu_(8)cluster supercrystals,Cu_(8)-1,Cu_(8)-2,and Cu_(8)-3,with homologous cluster molecule units[Cu_(8)(PNP)_(3)(EPPTA)_(6)](PF_(6))_(2)but distinct packing.These supercrystals display brightμs-long photoluminescence with a high quantum yield of up to 26.6%in solid-state at room temperature and aggregation-induced emission(AIE)characteristic.Superior thermal stability and blue-excitable bright yellow emission make Cu_(8)-3 serve as a yellow phosphor for white light-emitting diode.Furthermore,upon being stimulated by solvent vapor and temperature,reversible supercrystal-to-supercrystal transformations can be witnessed accompanied by remarkable color and luminescence switching.This work not only provides a kind of Cu cluster supercrystal model but also motivates the further development of metal clusters in multi-response materials.展开更多
基金supported by the National Natural Science Foundation of China,Nos.82171363,82371381(to PL),82171458(to XJ)Key Research and Development Project of Shaa nxi Province,Nos.2024SF-YBXM-404(to KY)。
文摘Recent advances in research on extracellular vesicles have significantly enhanced their potential as therapeutic agents for neurological diseases.Owing to their therapeutic properties and ability to cross the blood–brain barrier,extracellular vesicles are recognized as promising drug delivery vehicles for various neurological conditions,including ischemic stroke,traumatic brain injury,neurodegenerative diseases,glioma,and psychosis.However,the clinical application of natural extracellular vesicles is hindered by their limited targeting ability and short clearance from the body.To address these limitations,multiple engineering strategies have been developed to enhance the targeting capabilities of extracellular vesicles,thereby enabling the delivery of therapeutic contents to specific tissues or cells.Therefore,this review aims to highlight the latest advancements in natural and targeting-engineered extracellular vesicles,exploring their applications in treating traumatic brain injury,ischemic stroke,Parkinson's disease,Alzheimer's disease,amyotrophic lateral sclerosis,glioma,and psychosis.Additionally,we summarized recent clinical trials involving extracellular vesicles and discussed the challenges and future prospects of using targeting-engineered extracellular vesicles for drug delivery in treating neurological diseases.This review offers new insights for developing highly targeted therapies in this field.
基金supported by funding from the Medical Research Funding of Guangdong(No.A2022499 to QGX)the National Natural Science Foundation of China(No.82301796 to PL)the Guangdong Province Regional Joint Fund-Youth Fund Project of China(No.2022A1515111201 to PL).
文摘This study aimed to introduce a modified Byars staged procedure and investigate its application value in patients with severe hypospadias.We retrospectively analyzed the clinical data of patients with severe hypospadias admitted to the First Affiliated Hospital of Sun Yat-sen University(Guangzhou,China)between October 2012 and October 2022.In total,31 patients underwent the conventional Byars procedure(conventional group),and 45 patients underwent the modified Byars staged procedure(modified group).Our modified strategy was built upon the standard Byars procedure by incorporating glansplasty during the first stage and employing a Y-shaped flap in conjunction with a glandular tunnel for urethroplasty during the second stage.Notably,there were no statistically significant differences in the preoperative baseline characteristics,duration of surgery,amount of blood loss,or occurrence of postoperative complications,including urethral fistula,stricture and diverticulum,or penile curvature,between the conventional and modified groups.However,there was a significantly lower incidence of coronal sulcus fistula(0 vs 16.1%,P=0.02)and glans dehiscence(0 vs 12.9%,P=0.02)in the surgical group than that in the conventional group.In addition,the modified group exhibited a notably greater rate of normotopic urethral opening(100.0%vs 83.9%,P=0.01)and a higher mean score on the Hypospadias Objective Penile Evaluation(HOPE;mean±standard error of mean:8.6±0.2 vs 7.9±0.3,P=0.02)than did the conventional group.In conclusion,the modified Byars staged procedure significantly reduced the risks of glans dehiscence and coronal sulcus fistula.Consequently,it offers a promising approach for achieving favorable penile esthetics,thereby providing a reliable therapeutic option for severe hypospadias.
基金financially supported by the JSPS KAKENHI Grant No.22H01855,Japan.Ziyuan Yang,Xiaowei An,Zhongbao Feng,Peng LuoChangrui Feng gratefully acknowledge the China Scholarship Council(CSC),China.Yusrin Ramli greatly acknowledges the MEXT of Japan for the scholarship.Numerical calculations were conducted at the Hefei Advanced Computing Center in China.The authors also acknowledge the Shared Facility Center for Science and Technology(SFCST)at Hirosaki University,Japan,for XRD,SEM-EDS,and TEM-EDS measurements.
文摘Electrocatalytic reduction of carbon dioxide(CO_(2))with high selectivity to generate value-added chemical products and fuels,utilizing renewable electricity sources,offers an effective approach to address the continued increase in atmospheric CO_(2).Here,we report cerium(Ce)-doped indium oxide(In2O3)electrocatalyst generated in situ on carbon paper(Ce-In_(2)O_(3)/CP),and its application in the electrochemical reduction of CO_(2)(CO_(2)RR)to formate formation with high Faradaic efficiency(FE)(reaching 97.6%at−1.7 V vs.Ag/AgCl)and excellent stability.Experimental analysis and density functional theory(DFT)calculations indicate that doping Ce onto the(222)plane of In_(2)O_(3)induces lattice distortion,which promotes electron transfer from Ce to In while adjusting the local electronic structure of the In atoms around Ce,making them more favorable for the adsorption of*OOCH intermediates and effectively lowering the energy barrier of the rate-determining step.Furthermore,Ce doping lowers the overpotential required for formate production and suppresses the hydrogen evolution reaction(HER),effectively enhancing the selectivity of formate.
文摘Roads inevitably have defects during use,which not only seriously affect their service life but also pose a hidden danger to traffic safety.Existing algorithms for detecting road defects are unsatisfactory in terms of accuracy and generalization,so this paper proposes an algorithm based on YOLOv11.The method embeds wavelet transform convolution(WTConv)into the backbone’s C3k2 module to enhance low-frequency feature extraction while avoiding parameter bloat.Secondly,a novel multi-scale fusion diffusion network(MFDN)architecture is designed for the neck to strengthen cross-scale feature interactions,boosting detection precision.In terms of model optimization,the traditional downsampling method is discarded,and the innovative Adown(adaptive downsampling)technique is adopted,which streamlines the parameter scales while effectively mitigating the information loss problem during downsampling.Finally,in this paper,we propose Wise-PIDIoU by combining WiseIoU and MPDIoU to minimize the negative impact of low-quality anchor frames and enhance the detection capability of the model.The experimental results indicate that the proposed algorithm achieves an average detection accuracy of 86.5%for mAP@50 on the RDD2022 dataset,which is 2%higher than the original algorithm while ensuring that the amount of computation is basically unchanged.The number of parameters is reduced by 17%,and the F1 score is improved by 3%,showing better detection performance than other algorithms when facing different types of defects.The excellent performance on embedded devices proves that the algorithm also has favorable application prospects in practical inspection.
文摘It is convincingly demonstrated by numerous studies that the self-similarity of modern multimedia network traffic is presented by Hurst parameter (H). The specific performance is that the similar degree is higher along with the increase of H when H is between 0.5 and 1. However, it is doubtable that whether the complicated process of self-similarity can be described comprehensively by the parameter H only. Therefore, another important parameter cf has been proposed based on the discrete wavelet decomposition in this paper. The significance of the parameters is provided and the performance of the self-similarity process is described better.
文摘To ensure the efficient operation and timely maintenance of wind turbines, thereby enhancing energy security, it is critical to monitor the operational status of wind turbines and promptly identify abnormal conditions. This process relies on data collected over time by turbine sensors, including measurements such as current, voltage, temperature, and vibration signals. However, in practical applications, data from normal and abnormal conditions often exhibit an imbalance in quantity, posing challenges to traditional anomaly detection methods. Additionally, sensor data inherently contains temporal information, making the effective extraction of time-dependent features another key challenge. To address these issues, this paper proposes an anomaly detection method for wind turbine operations based on an improved Auxiliary Classifier Generative Adversarial Network. The proposed approach first employs the latent features of the training samples to augment the dataset and subsequently utilizes a Long Short-Term Memory network discriminator to extract temporal features from the samples for classification. This process directly outputs the anomaly detection results for test samples. To validate the effectiveness of the proposed method, this study uses a wind turbine blade icing dataset obtained from a Supervisory Control and Data Acquisition system. The proposed method is compared with other commonly used anomaly detection approaches. The validation and comparison results demonstrate that the proposed method achieves the lowest false alarm and missed detection rates on the blade icing dataset, underscoring its superior performance in wind turbine anomaly detection.
基金supported by the National Natural Science Foundation of China(Nos.51171009 and 50971007)the Program for New Century Excellent Talents inUniversity(No.NCET-09-0024)
文摘NiTi shape memory alloy was modified by Ag ion implantation with different incident doses to improve its antibacterial property. The atomic force microscopy, auger electron spectroscopy, and X-ray photoelectron spectroscopy show that the surface of NiTi alloy is covered by TiO2 nano-film with embedded pure Ag with a peak concentration of 5.0 at% at the incident dose of 1.5 x10^17 ions.cm-a, and Ni concentration is reduced in the super- ficial surface layer. The surface roughness reaches the maximum value nearly twice higher than the control sample at the incident dose of 1.5x10^17 ions.cm-2. The potentiodynamic anodic polarization curves show that the Ag-implanted NiTi samples possess higher self-corrosion potential （Ecorr） and lower self-corrosion current density （icor0 but lower breakdown potential （Ebr）. Therefore, the corrosion resistance of the Ag-NiTi is comparable to, if not better than, the untreated NiTi. The antibacterial tests reveal that there is a distinct reduction of the germ numbers on the Ag-NiTi, which is due to the direct contact between Ag and germ, and enhanced by the leaching Ag ions.
基金supported by the National Key Scientific Project of China(No.2011ZX05005-0042016ZX05005-002)the National Basic Research Program of China(973 Program)(No.2012CB214806)
文摘Stable carbon isotope ratio （δ13Ccarb） analysis has been widely applied to the study of the inter-conti- nental or global marine carbonate correlation. Large-scale Cambrian-Ordovician carbonate platforms were developed in the Tarim Basin. But research on fluctuation character- istics and global correlation of δ13Ccarb is still weak. Based on conodont biostratigraphy and whole-rock δ13Ccarb data in the Tahe oil-gas field of the northern Tarim Basin, the global correlation and genesis of positive carbon isotope excursions in the Darriwilian--Early Katian was exam- ined. Three positive excursions were identified in the Tahe oil-gas field including the middle Darriwilian carbon iso- tope excursion （MDICE）, the Guttenberg carbon isotope excursion （GICE）, and a positive excursion within the Pygodus anserinus conodont zone which is named the Early Sandbian carbon isotope excursion （ESICE） in this paper. Furthermore, these positive excursions had no direct relation with sea level fluctuations. MDICE and GICE could be globally correlated. The Middle-Upper Ordovi- cian Saergan Formation source rocks of the Kalpin outcrops were in accordance with the geological time of MDICE and ESICE. GICE had close relationship with the source rock of the Lianglitag Formation in the basin.Massive organic carbon burial was an important factor controlling the genesis of these positive excursions.
基金supported by grants from the National Natural Science Foundation of China,No.30170962the Major Subject of Key Technology of Guangzhou City of China,No.2002Z1-E0031science and technology projects of Nanshan district,No.2014028
文摘Acellular nerve allografts can help preserve normal nerve structure and extracellular matrix composition. These allografts have low immunogenicity and are more readily available than autologous nerves for the repair of long-segment peripheral nerve defects. In this study, we repaired a 40-mm ulnar nerve defect in rhesus monkeys with tissue-engineered peripheral nerve, and compared the outcome with that of autograft. The graft was prepared using a chemical extract from adult rhesus monkeys and seeded with allogeneic Schwann cells. Pathomo- rphology, electromyogram and immunohistochemistry findings revealed the absence of palmar erosion or ulcers, and that the morphology and elasticity of the hypothenar eminence were normal 5 months postoperatively. There were no significant differences in the mean peak compound muscle action potential, the mean nerve conduction velocity, or the number of neurofilaments between the experimental and control groups. However, outcome was significantly better in the experimental group than in the blank group. These findings suggest that chemically extracted allogeneic nerve seeded with autologous Schwann cells can repair 40-mm ulnar nerve defects in the rhesus monkey. The outcomes are similar to those obtained with autologous nerve graft.
基金financially supported by the National Oil and Gas Major Project(2016ZX05047-003,2016ZX05014002-006)the National Natural Science Foundation of China(41572124)the Fundamental Research Funds for the Central Universities(17CX05010)
文摘Analysis of the in situ stress orientation and magnitude in the No.4 Structure of Nanpu Sag was performed on the basis of data obtained from borehole breakout and acoustic emission measurements.On the basis of mechanical experiments,logging interpretation,and seismic data,a 3 D geological model and heterogeneous rock mechanics field of the reservoir were constructed.Finite element simulation techniques were then used for the detailed prediction of the 3 D stress field.The results indicated that the maximum horizontal stress orientation in the study area was generally NEE-SWW trending,with significant changes in the in situ stress orientation within and between fault blocks.Along surfaces and profiles,stress magnitudes were discrete and the in situ stress belonged to theⅠa-type.Observed inter-strata differences were characterized as five different types of in situ stress profile.Faults were the most important factor causing large distributional differences in the stress field of reservoirs within the complex fault blocks.The next important influence on the stress field was the reservoir’s rock mechanics parameters,which impacted on the magnitudes of in situ stress magnitudes.This technique provided a theoretical basis for more efficient exploration and development of low-permeability reservoirs within complex fault blocks.
基金the National Natural Science Foundation of China(No.81871110).
文摘Many therapies are effective in treating varicoceles,including dilation of the pampiniform plexus in males.The most common method of treatment is varicocelectomy.We aimed to assess an alternative technique(microsurgical spermatic[distal end]-superficial or inferior epigastric vein anastomosis)that preserves the normal blood flow pattern for varicocele treatment.We retrospectively analyzed 27 men with varicocele between October 2019 and July 2020.All patients underwent microsurgical spermatic(distal end)-superficial or inferior epigastric vein anastomosis.The prognosis was reviewed retrospectively with an additional survey conducted 3 months after surgery.The mean±standard deviation of the age was 26.1±7.3 years in patients with microsurgical spermatic(distal end)-superficial or inferior epigastric vein anastomosis.The maximum diameter of the varicocele vein,perineal pain score,sperm density,and forward movement of sperm improved over 3 months after surgery.Microsurgical spermatic(distal end)-superficial or inferior epigastric vein anastomosis is a safe and efficient surgical treatment for varicoceles.
基金supported by grants from the National Natural Science Foundation of China(No.81971314)。
文摘Repairing glans dehiscence after failed hypospadias repair is challenging for pediatric surgeons.Here,we introduced and evaluated a newly modified Mathieu technique,Mathieu combined tunnel(MCT),which involves multiple custom-designed flaps for the shortage of flap source material after repeated operations;we also constructed a tunnel to avoid the glans incision that may carry new risks of dehiscence.This retrospective study included 26 patients who were consecutively admitted to the First Affiliated Hospital of Sun Yat-Sen University(Guangzhou,China)for glans dehiscence repair after failed hypospadias repair from October 2014 to October 2020;sixteen patients underwent surgery using the MCT(MCT group)and ten patients underwent surgery using the tubularized incised plate(TIP)technique(TIP group).The operative time,blood loss,postoperative complications,normal urethral meatus rate,success rate,and Hypospadias Objective Penile Evaluation(HOPE)score were compared between the two groups.The MCT group achieved an overall satisfactory penile appearance and voiding function,with a higher rate of normal urethral meatus(15/16,93.8%)and a lower rate of glans dehiscence(1/16,6.2%),compared with the TIP group(70.0%and 30.0%,respectively).However,these differences were not statistically significant,possibly because of the limited number of patients(all P>0.05).Mean postoperative HOPE scores were similar in the MCT group(mean±standard deviation:8.83±0.89)and TIP group(8.94±0.57)(P>0.05).No significant differences were found between the two groups in terms of blood loss and success rate,nor in the rates of various complications(e.g.,fistula,urethral stricture,and glans dehiscence).In conclusion,the MCT technique appears to be feasible and reliable for repairing glans dehiscence after failed hypospadias repair.
基金supported by the National Natural Science Foundation of China,No.30571913a grant from the Science and Technology Project of Guangdong Province of China,No.2013B010404019+1 种基金the Natural Science Foundation of Guangdong Province of China,No.9151008901000006the Medical Scientific Research Foundation of Guangdong Province of China,No.A2009173
文摘The three-dimensional（3D） visualization of the functional bundles in the peripheral nerve provides direct and detailed intraneural spatial information. It is useful for selecting suitable surgical methods to repair nerve defects and in optimizing the construction of tissue-engineered nerve grafts. However, there remain major technical hurdles in obtaining, registering and interpreting 2D images, as well as in establishing 3D models. Moreover, the 3D models are plagued by poor accuracy and lack of detail and cannot completely reflect the stereoscopic microstructure inside the nerve. To explore and help resolve these key technical problems of 3D reconstruction, in the present study, we designed a novel method based on re-imaging techniques and computer image layer processing technology. A 20-cm ulnar nerve segment from the upper arm of a fresh adult cadaver was used for acetylcholinesterase（ACh E） staining. Then, 2D panoramic images were obtained before and after ACh E staining under the stereomicroscope. Using layer processing techniques in Photoshop, a space transformation method was used to fulfill automatic registration. The contours were outlined, and the 3D rendering of functional fascicular groups in the long-segment ulnar nerve was performed with Amira 4.1 software. The re-imaging technique based on layer processing in Photoshop produced an image that was detailed and accurate. The merging of images was accurate, and the whole procedure was simple and fast. The least square support vector machine was accurate, with an error rate of only 8.25%. The 3D reconstruction directly revealed changes in the fusion of different nerve functional fascicular groups. In conclusion. The technique is fast with satisfactory visual reconstruction.
文摘Spermatogenic damage may occur after vasectomy, and the damage is pressure mediated, occurring when the occluded reproductive tract is unable to accommodate additional sperm produced by the testis. This study aimed to determine the long-term effect of vasectomy on spermatogenesis in humans and clarify how the balance between sperm production in the testis and sperm storage in or removal from the tract might be maintained. During inguinal hernia repair, an open biopsy was performed to obtain testicular tissue blocks from 51 Chinese men （aged ≥ 50 years）, of whom 25 （control group） had not undergone vasectomy and 26 （vasectomized group） had undergone bilateral vasectomy 22-42 years before. Methacrylate resin-embedded testicular sections were made, and morphometric studies were performed using light microscopy. In addition, sizes of the testis and epididymis were estimated with ultrasonography. The testicular tissue blocks obtained from one control and seven vasectomized men consisted almost completely of connective tissue. In the other 43 men, significant differences were not found between the two groups in the testicular or epididymal size, qualitative histology or quantitative parameters including the mean diameter or volume fraction of the seminiferous tubules. In conclusion, sperm production and sperm storage/removal reached a static equilibrium after vasectomy, likely due to spermatogenic degeneration or less sperm production as a result of aging or due to vasectomy-induced testicular （interstitial） fibrosis. Thus, complications that might occur in association with overproduction of sperm and distension of the tract would disappear or be relieved with time.
基金the National Natural Science Foundation of China (51676090)the Natural Science Foundation of Jiangsu Province (BK20150513), and the Six Talent Peaks Project in Jiangsu Province.
文摘In this investigation, Pt–Ba–Ce/c-Al2O3 catalysts were prepared by incipient wetness impregnation and experiments were performed to evaluate the influence of H2 on the evolution mechanism of nitrogen oxides (NOx) storage and reduction (NSR). The physical and chemical properties of the Pt–Ba–Ce/c- Al2O3 catalysts were studied using a combination of characterization techniques, which showed that PtOx, CeO2, and BaCO3, whose peaks were observed in X-ray diffraction (XRD) spectra, dispersed well on the c-Al2O3, as shown by transmission electron microscope (TEM), and that the difference between Ce3+ and Ce4+, as detected by X-ray photoelectron spectroscopy (XPS), facilitated the migration of active oxygen over the catalyst. In the process of a complete NSR experiment, the NOx storage capability was greatly enhanced in the temperature range of 250–350℃, and reached a maximum value of 315.3μmol·gcat^-1 at 350℃, which was ascribed to the increase in NO2 yield. In a lean and rich cycling experiment, the results showed that NOx storage efficiency and conversion were increased when the time of H2 exposure (i.e., 30, 45, and 60 s) was extended. The maximum NOx conversion of the catalyst reached 83.5% when the duration of the lean and rich phases was 240 and 60 s, respectively. The results revealed that increasing the content of H2 by an appropriate amount was favorable to the NSR mechanism due to increased decomposition of nitrate or nitrite, and the refreshing of trapping sites for the next cycle of NSR.
基金support provided by the National Natural Science Foundation of China (No. 51171009)the National Basic Research Program of China (No. 2012CB619403)
文摘The Ti-49.8at%Ni alloy was modified by Ti ion implantation to improve its corrosion resistance and biocompatibility. The chemical composition and morphologies of the Ti Ni alloy surface were determined using atomic force microscopy(AFM), auger electron spectroscopy(AES), and X-ray photoelectron spectroscopy(XPS). The results revealed that Ti ion implantation caused the reduction of Ni concentration and the formation of a Ti O2 nano-film on the Ti Ni alloy. The phase transformation temperatures of the Ti–Ti Ni alloy remained almost invariable after Ti ion implantation. Electrochemical tests indicated that the corrosion resistance of Ti Ni increased after Ti ion implantation. Moreover, the Ni ion release rate in 0.9% Na Cl solution for the Ti Ni alloy remarkably decreased due to the barrier effect of the Ti O2 nano-film. The cell proliferation behavior on Ti-implanted Ti Ni was better than that on the untreated Ti Ni after cell culture for 1 d and 3 d.
基金supported by the National Natural Science Foundation of China(81430043,81771239,81974188,and 81901186).
文摘Acute hypobaric hypoxic brain damage is a potentially fatal high-altitude sickness.Autophagy plays a critical role in ischemic brain injury,but its role in hypo-baric hypoxia(HH)remains unknown.Here we used an HH chamber to demonstrate that acute HH exposure impairs autophagic activity in both the early and late stages of the mouse brain,and is partially responsible for HH-induced oxidative stress,neuronal loss,and brain damage.The autophagic agonist rapamycin only promotes the initiation of autophagy.By proteome analysis,a screen showed that protein dynamin2(DNM2)potentially regulates autophagic flux.Overexpression of DNM2 significantly increased the formation of autolysosomes,thus maintaining autophagic flux in combination with rapamycin.Furthermore,the enhancement of autophagic activity attenuated oxidative stress and neurological deficits after HH exposure.These results contribute to evidence supporting the conclusion that DNM2-mediated autophagic flux represents a new therapeutic target in HH-induced brain damage.
基金supported by the National Natural Science Foundation of China(Nos.92061201,U21A20277,21825106,21975065,22205212)Project funded by China Postdoctoral Science Foundation(Nos.2021TQ0291,2022M722863)Postdoctoral Science Foundation of Zhengzhou University。
文摘Multi-response metal cluster supercrystal materials,which can simultaneously display various such as color,photoluminescence,changes by bearing only one stimulus,have huge potential as stimuliresponsive intelligent material,but are rarely reported.Here,we report three Cu_(8)cluster supercrystals,Cu_(8)-1,Cu_(8)-2,and Cu_(8)-3,with homologous cluster molecule units[Cu_(8)(PNP)_(3)(EPPTA)_(6)](PF_(6))_(2)but distinct packing.These supercrystals display brightμs-long photoluminescence with a high quantum yield of up to 26.6%in solid-state at room temperature and aggregation-induced emission(AIE)characteristic.Superior thermal stability and blue-excitable bright yellow emission make Cu_(8)-3 serve as a yellow phosphor for white light-emitting diode.Furthermore,upon being stimulated by solvent vapor and temperature,reversible supercrystal-to-supercrystal transformations can be witnessed accompanied by remarkable color and luminescence switching.This work not only provides a kind of Cu cluster supercrystal model but also motivates the further development of metal clusters in multi-response materials.
