目的:研究扩心方对阿霉素诱导扩张型心肌病模型大鼠心功能、心肌损伤的保护作用,探讨扩心方作用机制。方法:50只Wistar大鼠腹腔注射阿霉素6周建立DCM模型,开始造模4周后随机分为模型组,扩心方高、中、低剂量组,卡托普利组(每组各10只),...目的:研究扩心方对阿霉素诱导扩张型心肌病模型大鼠心功能、心肌损伤的保护作用,探讨扩心方作用机制。方法:50只Wistar大鼠腹腔注射阿霉素6周建立DCM模型,开始造模4周后随机分为模型组,扩心方高、中、低剂量组,卡托普利组(每组各10只),连续灌胃给药四周,另设10只正常对照。正常组和模型组以生理盐水灌胃。治疗结束后,对各组大鼠进行心脏超声检查,病理形态学检测,以及用反转录-聚合酶链反应和蛋白质免疫印迹法分别检测心肌组织中SERCA2α、PLB的m RNA和蛋白含量。结果:与正常对照组相比,模型组心功能减弱,出现心肌病理损害,SERCA2α与PLB表达异常;与模型组相比,扩心方组可以改善DCM大鼠的心功能、心肌病理学形态,同时,提高SERCA2αm RNA和蛋白表达、抑制PLB m RNA和蛋白表达。结论:扩心方能提高DCM大鼠的左心室射血分数和缩短率,减轻心肌损伤,改善心功能,同时调控DCM大鼠SERCA2α、PLB的表达。提示扩心方改善DCM大鼠心功能的作用机制,可能与纠正心肌肌浆网钙调控相关蛋白-SERCA2α、PLB的异常表达相关。展开更多
目的:探究潼白蒺藜对高脂血症模型大鼠血脂水平的影响及机制,为药对协同降血脂的临床应用提供参考。方法:48只SPF级SD大鼠随机等分为正常对照组,高脂模型组,辛伐他汀组、潼白蒺藜药对组,并设白蒺藜组,潼蒺藜组对比。正常对照组采用基础...目的:探究潼白蒺藜对高脂血症模型大鼠血脂水平的影响及机制,为药对协同降血脂的临床应用提供参考。方法:48只SPF级SD大鼠随机等分为正常对照组,高脂模型组,辛伐他汀组、潼白蒺藜药对组,并设白蒺藜组,潼蒺藜组对比。正常对照组采用基础饲料喂养,其余各组采用高脂饲料饲喂法建立高脂血症大鼠模型,造模的同时预防性给药,用潼蒺藜水提物、白蒺藜水提物、潼白蒺藜药对水提物和阳性对照药辛伐他汀分别灌胃,6周后检测大鼠体重及各脏器指数、并检测血清总胆固醇(Total cholesterol,TC)、甘油三酯(Triglyceride,TG)、高密度脂蛋白(high density lipoprotein-cholesterol,HDL-C)、低密度脂蛋白(high density lipoprotein-cholesterol,LDL-C)的含量,RT-PCR及western blot检测各组肝脏组织三羟基三甲基戊二酸单酰辅酶A还原酶(3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase,HMGCR)、胆固醇7a羟化酶(Cytochrome P450 7A1,CYP7A1)、低密度脂蛋白受体(Low-Density Lipoprotein Receptor,LDL-R)mRNA及蛋白表达。结果:三组中药组均不同程度降低高脂模型大鼠血液TC、TG、LDL-C含量,提高HDL-C含量,其中潼白蒺藜药对更加显著(P<0.01);三组中药组均能提高脂模型大鼠肝脏HMGCR、CYP7A1、LDL-R基因及蛋白表达,潼白蒺藜药对组疗效更为显著。结论:潼白蒺藜药对能显著调节机体的血脂水平,有效预防高脂血症,发挥协同增效作用,且其机制可能与调节HMGCR、CYP7A1、LDL-R表达有关。展开更多
OBJECTIVE: To investigate the effects of Ningmitai capsule combined with sertraline on patients with premature ejaculation(PE) and an increased anterior-posterior diameter(APD) of the seminal vesicles(SVs).METHODS: Si...OBJECTIVE: To investigate the effects of Ningmitai capsule combined with sertraline on patients with premature ejaculation(PE) and an increased anterior-posterior diameter(APD) of the seminal vesicles(SVs).METHODS: Sixty men with acquired PE were enrolled and randomly divided into two groups. The combined group was treated with Ningmitai capsule and sertraline, while the control group was treated with sertraline alone. Main outcomes were measured using the premature ejaculation diagnostic tool(PEDT), APD of SVs, and Clinical Global Impression of Change questionnaire and compared before and after 3 months of treatment.RESULTS: Comparing after treatment with before treatment outcomes within each group, the PEDT score was significantly reduced in the combined group(12.1 ± 2.5 vs 8.6 ± 3.2, P < 0.001, respectively) and control group(12.9 ± 2.6 vs 10.3 ± 1.6, P <0.001, respectively). Furthermore, the PEDT score after treatment was significantly lower in the combined compared with control group(8.6 ± 3.2 vs10.3 ± 1.6, P = 0.011, respectively). The APD of SVs in the combined group was significantly decreased after treatment [(10.8 ± 2.4) vs(12.9 ± 2.2) mm, P =0.001], while the APD of SVs in the control group was equivalent before and after treatment. The treatment response rate was not significantly higher in the combined compared with control group.CONCLUSION: These results indicated that the effect of Ningmitai capsule combined with sertraline was better than that of sertraline alone for the treatment of PE patients exhibiting an increased APD of SVs. The therapeutic effect found for the combined treatment may be due to antibacterial and anti-inflammatory activity reported for Ningmitai capsule,and may suggest that seminal vesiculitis is a potential pathophysiological factor in acquired PE.展开更多
文摘目的:研究扩心方对阿霉素诱导扩张型心肌病模型大鼠心功能、心肌损伤的保护作用,探讨扩心方作用机制。方法:50只Wistar大鼠腹腔注射阿霉素6周建立DCM模型,开始造模4周后随机分为模型组,扩心方高、中、低剂量组,卡托普利组(每组各10只),连续灌胃给药四周,另设10只正常对照。正常组和模型组以生理盐水灌胃。治疗结束后,对各组大鼠进行心脏超声检查,病理形态学检测,以及用反转录-聚合酶链反应和蛋白质免疫印迹法分别检测心肌组织中SERCA2α、PLB的m RNA和蛋白含量。结果:与正常对照组相比,模型组心功能减弱,出现心肌病理损害,SERCA2α与PLB表达异常;与模型组相比,扩心方组可以改善DCM大鼠的心功能、心肌病理学形态,同时,提高SERCA2αm RNA和蛋白表达、抑制PLB m RNA和蛋白表达。结论:扩心方能提高DCM大鼠的左心室射血分数和缩短率,减轻心肌损伤,改善心功能,同时调控DCM大鼠SERCA2α、PLB的表达。提示扩心方改善DCM大鼠心功能的作用机制,可能与纠正心肌肌浆网钙调控相关蛋白-SERCA2α、PLB的异常表达相关。
文摘目的:探究潼白蒺藜对高脂血症模型大鼠血脂水平的影响及机制,为药对协同降血脂的临床应用提供参考。方法:48只SPF级SD大鼠随机等分为正常对照组,高脂模型组,辛伐他汀组、潼白蒺藜药对组,并设白蒺藜组,潼蒺藜组对比。正常对照组采用基础饲料喂养,其余各组采用高脂饲料饲喂法建立高脂血症大鼠模型,造模的同时预防性给药,用潼蒺藜水提物、白蒺藜水提物、潼白蒺藜药对水提物和阳性对照药辛伐他汀分别灌胃,6周后检测大鼠体重及各脏器指数、并检测血清总胆固醇(Total cholesterol,TC)、甘油三酯(Triglyceride,TG)、高密度脂蛋白(high density lipoprotein-cholesterol,HDL-C)、低密度脂蛋白(high density lipoprotein-cholesterol,LDL-C)的含量,RT-PCR及western blot检测各组肝脏组织三羟基三甲基戊二酸单酰辅酶A还原酶(3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase,HMGCR)、胆固醇7a羟化酶(Cytochrome P450 7A1,CYP7A1)、低密度脂蛋白受体(Low-Density Lipoprotein Receptor,LDL-R)mRNA及蛋白表达。结果:三组中药组均不同程度降低高脂模型大鼠血液TC、TG、LDL-C含量,提高HDL-C含量,其中潼白蒺藜药对更加显著(P<0.01);三组中药组均能提高脂模型大鼠肝脏HMGCR、CYP7A1、LDL-R基因及蛋白表达,潼白蒺藜药对组疗效更为显著。结论:潼白蒺藜药对能显著调节机体的血脂水平,有效预防高脂血症,发挥协同增效作用,且其机制可能与调节HMGCR、CYP7A1、LDL-R表达有关。
基金Supported by a Grant from the National Key Basic Research Program of China(No.2013CB945204)a Research and Development Project for Young Doctors in Reproductive Medicine from the Chinese Medical Association Specialized Fund for Clinical Medical Research(No.16020380654)
文摘OBJECTIVE: To investigate the effects of Ningmitai capsule combined with sertraline on patients with premature ejaculation(PE) and an increased anterior-posterior diameter(APD) of the seminal vesicles(SVs).METHODS: Sixty men with acquired PE were enrolled and randomly divided into two groups. The combined group was treated with Ningmitai capsule and sertraline, while the control group was treated with sertraline alone. Main outcomes were measured using the premature ejaculation diagnostic tool(PEDT), APD of SVs, and Clinical Global Impression of Change questionnaire and compared before and after 3 months of treatment.RESULTS: Comparing after treatment with before treatment outcomes within each group, the PEDT score was significantly reduced in the combined group(12.1 ± 2.5 vs 8.6 ± 3.2, P < 0.001, respectively) and control group(12.9 ± 2.6 vs 10.3 ± 1.6, P <0.001, respectively). Furthermore, the PEDT score after treatment was significantly lower in the combined compared with control group(8.6 ± 3.2 vs10.3 ± 1.6, P = 0.011, respectively). The APD of SVs in the combined group was significantly decreased after treatment [(10.8 ± 2.4) vs(12.9 ± 2.2) mm, P =0.001], while the APD of SVs in the control group was equivalent before and after treatment. The treatment response rate was not significantly higher in the combined compared with control group.CONCLUSION: These results indicated that the effect of Ningmitai capsule combined with sertraline was better than that of sertraline alone for the treatment of PE patients exhibiting an increased APD of SVs. The therapeutic effect found for the combined treatment may be due to antibacterial and anti-inflammatory activity reported for Ningmitai capsule,and may suggest that seminal vesiculitis is a potential pathophysiological factor in acquired PE.
