Delayed or non-healing of diabetic wounds is a significant complication,often attributed to high glucose-induced M1 macrophage accumulation,impaired angiogenesis,and reactive oxygen species(ROS)buildup.Addressing this...Delayed or non-healing of diabetic wounds is a significant complication,often attributed to high glucose-induced M1 macrophage accumulation,impaired angiogenesis,and reactive oxygen species(ROS)buildup.Addressing this,we introduced a strontium polyphenol network microneedle patch(SrC-MPNs@MN-PP)for percutaneous drug delivery.This patch,formulated with polymer poly(γ-glutamic acid)(γ-PGA)and epsilon-poly-l-lysine(ε-PLL),incorporates strontium polyphenol networks(SrC-MPNs).The release of chlorogenic acid(CGA)from SrC-MPNs not only neutralizes ROS,but strontium ions also foster angiogen-esis.Consequently,SrC-MPNs@MN-PP can ameliorate the diabetic wound microenvironment and expedite healing.展开更多
This paper discusses the mechanism of insulin resistance in obesity from the research progress of Chinese and Western medicine and its oxidative stress,inflammatory reaction and abnormal lipid metabolism in cognitive ...This paper discusses the mechanism of insulin resistance in obesity from the research progress of Chinese and Western medicine and its oxidative stress,inflammatory reaction and abnormal lipid metabolism in cognitive dysfunction.It also reviews the research progress of massage,one of the external treatment methods of traditional Chinese medicine,in the treatment of insulin resistance and cognitive dysfunction in obesity,in order to provide more new ideas and new ways for clinical diagnosis and treatment of insulin resistance and related cognitive dysfunction caused by obesity.展开更多
Artificial intelligence is a frontier science for simulating,extending and expanding human intelligence,theoretical methods,technologies and application systems.Through the survey and analysis of the current situation...Artificial intelligence is a frontier science for simulating,extending and expanding human intelligence,theoretical methods,technologies and application systems.Through the survey and analysis of the current situation of the integration of artificial intelligence into the construction of the teacher team,the corresponding research plan and path are formulated,and the artificial intelligence is fully integrated with the construction of the professional teacher team of acupuncture and tuina,and specific practices are carried out.The practical research path includes the construction of the corresponding artificial intelligence education analysis system and platform for the teaching staff,the formulation and practice of artificial intelligence training and training plans for the teaching staff,the daily intelligent education of the teaching staff and the sustainable improvement of lifelong intelligent education literacy.Through this study,it is hoped that the teaching ability and level of acupuncture and tuina teachers will be improved,and more new ideas and methods will be provided for the training and education of acupuncture and tuina professionals.展开更多
[Objectives] To study the effect of "acupressure therapy" on anti-inflammatory mechanism in obesity-induced IR rats. [Methods] 10 rats were randomly selected and fed with normal diet as blank group, and the ...[Objectives] To study the effect of "acupressure therapy" on anti-inflammatory mechanism in obesity-induced IR rats. [Methods] 10 rats were randomly selected and fed with normal diet as blank group, and the remaining 30 rats were fed with high-fat diet for 8 weeks. The obesity-induced IR model was induced and divided into two groups: model group and acupressure group. The acupressure group was treated by "acupressure therapy" three times a week for 6 weeks;the model group was taken 3 times at the same time every week, and the supine binding experiment lasted for 10 min for 6 weeks. Morphological observation was carried out before and after the experiment, mainly observing the general conditions and body weight. After the last intervention, the animals were killed and samples were collected. Western Blot method was used to detect the expression of TNF-α and IL-6 protein in adipose tissue of the three groups. [Results] Compared with the blank group, the expression of TNF-α and IL-6 protein in adipose tissue of the model group increased significantly ( P <0.01);compared with the model group, the expression of TNF-α and IL-6 protein in adipose tissue of the acupressure group decreased significantly ( P <0.01). [Conclusions] After intervening in the obesity-induced IR rats with acupressure method, the status of insulin resistance in obese rats was improved by inhibiting the expression of inflammatory factors.展开更多
Skin wounds are common in accidental injuries,and the intricacies of wound repair are closely linked to endogenous electric fields.Electrical stimulation plays a pivotal role in the restorative processes of skin injur...Skin wounds are common in accidental injuries,and the intricacies of wound repair are closely linked to endogenous electric fields.Electrical stimulation plays a pivotal role in the restorative processes of skin injuries,encompassing collagen deposition,angiogenesis,inflammation,and re-epithelialization.Employing electrical stimulation therapy replicates and enhances the effects of endogenous wound electric fields by applying an external electric field to the wound site,thereby promoting skin wound healing.In this study,we developed a self-powered repetitive mechanical impacts-electrical stimulation(RMI-ES)system utilizing a BaTiO_(3)/polydimethylsiloxane(PDMS)piezoelectric composite film.Compared to conventional electrical stimulation devices,the fabricated piezoelectric composite film efficiently harvests energy from the pressure applied by the stimulation device and the tensile force occurring during natural rat activities.The results demonstrated that piezoelectric stimulation generated by the composite membrane expedited the cell cycle,promoting fibroblast proliferation.Additionally,piezoelectric stimulation induced favorable changes in fibroblast gene expression,including increased expression of transforming growth factor-β1(TGF-β1),connective tissue growth factor(CTGF),collagen 1,collagen 3,vascular endothelial growth factor(VEGF),and alpha-smooth muscle actin(α-SMA),while reducing interleukin-6(IL-6)expression.Transcriptome analysis revealed that piezoelectric stimulation may induce fibroblast migration,proliferation,and collagen expression by influencing PI3K/AKT serine/threonine kinase(AKT)pathways.Further confirmation through the addition of the PI3K inhibitor LY294002 validated that piezoelectric stimulation can regulate the repair process after skin injury through the pathway.Importantly,in vivo results demonstrated that the electric field at the wound site effectively promoted wound healing,reduced inflammation,and stimulated collagen deposition and neovascularization.This study emphasizes the role of the piezoelectric membrane as an effective,safe,and battery-free electrical stimulator crucial for skin wound healing.展开更多
Irrigation and fertilizer interaction is an efficient cultivation management strategy for facility agriculture.However,the effects of irrigation and fertilizer management on tomato growth and its physiological factors...Irrigation and fertilizer interaction is an efficient cultivation management strategy for facility agriculture.However,the effects of irrigation and fertilizer management on tomato growth and its physiological factors remain unclarified.In this study,two irrigation patterns(W1,conventional irrigation;W2,water-saving irrigation)and four fertilizer application patterns(CF,chemical fertilizer;BOF,biological organic fertilizer;NPK,nutrient compound fertilizer;BOF+NPK)were selected to observe the effects of their interaction on cherry tomato plant growth,leaf photosynthesis and fruit quality through pot experiments.The results showed that W2 treatments promoted plant height growth compared to W1 under the same fertilizer addition.Moreover,irrigation and fertilizer management had significant effects on net photosynthetic rate,intercellular oxidation concentration,stomatal conductance and transpiration rate at the first sequence flowering and fruiting stages.The maximum tomato plant height(99.0 cm)was achieved under the irrigation and fertilizer pattern of BOF and W2,along with the highest fruit yield of 1.98 kg/plant,which was approximately 31.1%higher than the minimum yield under the combined CF and W2 treatment.Under W2 treatments,the application of either NPK or BOF increased the soluble sugar content of tomatoes.The structural equation models showed that the soil alkali hydrolyzed nitrogen could directly significantly affect the yield and soluble sugar.The findings suggest that optimization of irrigation-fertilizer interactions positively regulates tomato growth,providing an efficient model for tomato irrigation and fertilizer management and a reference for sustainable development of facility agriculture.展开更多
Building self-assembly nanostructures is an important way to overcome the limitations of paclitaxel in tumor therapy.However,this strategy is also faced with challenges,such as difficulties in efficient release and th...Building self-assembly nanostructures is an important way to overcome the limitations of paclitaxel in tumor therapy.However,this strategy is also faced with challenges,such as difficulties in efficient release and the potential for drug resistance.Herein,we developed a near-infrared light-activatable melanized paclitaxel self-assembly nanoparticles for synergistic anti-tumor therapy.In this strategy,paclitaxel dimer prodrugs were synthesized and paclitaxel nanoparticles were obtained through self-assembly.Finally,the paclitaxel dimer nanoparticles were capped with polydopamine(PDA,melanoidin)and human serum albumin(HSA).The disulfide bonds in paclitaxel dimeric prodrug specifically respond to high concentrations of glutathione(GSH)and reactive oxygen species(ROS)in tumor cells.PDA enhances the biocompatibility of the drug molecules and imparts near-infrared photothermal conversion capability to the nano-self-assemblies.Both the in vitro and in vivo experiments demonstrated that this paclitaxel nanoprodrug exhibited enhanced tumor therapeutic efficacy under near-infrared light irradiation.展开更多
Skin aging is a systematic and complex process,mainly including intrinsic aging caused by time change and extrinsic aging caused by environmental factors.Photoaging caused by ultraviolet(UV)irradiation is the principl...Skin aging is a systematic and complex process,mainly including intrinsic aging caused by time change and extrinsic aging caused by environmental factors.Photoaging caused by ultraviolet(UV)irradiation is the principle extrinsic factor of skin aging,which can directly or indirectly cause damage to DNA and other biomolecules,generate reactive oxygen species(ROS),and induce a decrease in collagen synthesis and other adverse effect.Herein,to address the causative factors and multiple side effects of skin photoaging,we constructed multifunctional peptide-melanin hybrid nanodots(PMHDs)to achieve multifaceted synergistic anti-skin aging by using melanin-like nanodots(MDs)and functional peptides as active motifs.Among them,the peptide has the activity of promoting collagen synthesis,while the MDs have the function of absorbing UV light and scavenging free radicals.Additionally,the MDs also act as a carrier to increase the cellular uptake of the peptide.The anti-photoaging effects of the PMHDs were systematically verified in a multidimensional manner at the biochemical,cellular and small animal levels,respectively.展开更多
基金supported by the National Natural Science Foundation of China(No.31971271)The Natural Science Foundation of Fujian Province of China(No.2022J01794)The Science and Technology Plan Project of Quanzhou(No.2021N033S).
文摘Delayed or non-healing of diabetic wounds is a significant complication,often attributed to high glucose-induced M1 macrophage accumulation,impaired angiogenesis,and reactive oxygen species(ROS)buildup.Addressing this,we introduced a strontium polyphenol network microneedle patch(SrC-MPNs@MN-PP)for percutaneous drug delivery.This patch,formulated with polymer poly(γ-glutamic acid)(γ-PGA)and epsilon-poly-l-lysine(ε-PLL),incorporates strontium polyphenol networks(SrC-MPNs).The release of chlorogenic acid(CGA)from SrC-MPNs not only neutralizes ROS,but strontium ions also foster angiogen-esis.Consequently,SrC-MPNs@MN-PP can ameliorate the diabetic wound microenvironment and expedite healing.
基金Supported by National Natural Science Foundation of China(82174525)Jilin Science and Technology Development Plan Project(YDZJ202201ZYTS195)Jilin Traditional Chinese Medicine Science and Technology Project in 2022(2022128).
文摘This paper discusses the mechanism of insulin resistance in obesity from the research progress of Chinese and Western medicine and its oxidative stress,inflammatory reaction and abnormal lipid metabolism in cognitive dysfunction.It also reviews the research progress of massage,one of the external treatment methods of traditional Chinese medicine,in the treatment of insulin resistance and cognitive dysfunction in obesity,in order to provide more new ideas and new ways for clinical diagnosis and treatment of insulin resistance and related cognitive dysfunction caused by obesity.
基金Supported by National TCM Examination Scientific Research Project in 2021(TC2021022)Jilin Province Vocational Education and Adult Education Teaching Reform Research Project in 2022(2022ZCY295)+3 种基金Higher Education Scientific Research Project of Jilin Association for Higher Education in 2021(JGJX2021D170)Jilin Province Educational Science"13th Five-Year"Plan 2020 Annual Project(GH20169)Changchun University of Traditional Chinese Medicine Higher Education Teaching Research Project in 2022(XJSZ202240)Jilin Higher Education Scientific Research Project in 2022(JGJX2022D129)。
文摘Artificial intelligence is a frontier science for simulating,extending and expanding human intelligence,theoretical methods,technologies and application systems.Through the survey and analysis of the current situation of the integration of artificial intelligence into the construction of the teacher team,the corresponding research plan and path are formulated,and the artificial intelligence is fully integrated with the construction of the professional teacher team of acupuncture and tuina,and specific practices are carried out.The practical research path includes the construction of the corresponding artificial intelligence education analysis system and platform for the teaching staff,the formulation and practice of artificial intelligence training and training plans for the teaching staff,the daily intelligent education of the teaching staff and the sustainable improvement of lifelong intelligent education literacy.Through this study,it is hoped that the teaching ability and level of acupuncture and tuina teachers will be improved,and more new ideas and methods will be provided for the training and education of acupuncture and tuina professionals.
基金Supported by National Natural Science Foundation of China(82174525)Jilin Science and Technology Development Plan Project(YDZJ202201ZYTS195)+2 种基金Jilin Natural Science Foundation(YDZJ202301ZYTS469)Jilin Traditional Chinese Medicine Science and Technology Project in 2022(2022128)2023 Youth Outstanding Discipline Backbone Training Project of Changchun University of Chinese Medicine(202304).
文摘[Objectives] To study the effect of "acupressure therapy" on anti-inflammatory mechanism in obesity-induced IR rats. [Methods] 10 rats were randomly selected and fed with normal diet as blank group, and the remaining 30 rats were fed with high-fat diet for 8 weeks. The obesity-induced IR model was induced and divided into two groups: model group and acupressure group. The acupressure group was treated by "acupressure therapy" three times a week for 6 weeks;the model group was taken 3 times at the same time every week, and the supine binding experiment lasted for 10 min for 6 weeks. Morphological observation was carried out before and after the experiment, mainly observing the general conditions and body weight. After the last intervention, the animals were killed and samples were collected. Western Blot method was used to detect the expression of TNF-α and IL-6 protein in adipose tissue of the three groups. [Results] Compared with the blank group, the expression of TNF-α and IL-6 protein in adipose tissue of the model group increased significantly ( P <0.01);compared with the model group, the expression of TNF-α and IL-6 protein in adipose tissue of the acupressure group decreased significantly ( P <0.01). [Conclusions] After intervening in the obesity-induced IR rats with acupressure method, the status of insulin resistance in obese rats was improved by inhibiting the expression of inflammatory factors.
基金supported by the National Natural Science Foundation of China(Nos.31870967 to W.L.and 81701841 to W.B.W.)the National Key R&D Program of China(No.2018YFC1105800 to W.L.)。
文摘Skin wounds are common in accidental injuries,and the intricacies of wound repair are closely linked to endogenous electric fields.Electrical stimulation plays a pivotal role in the restorative processes of skin injuries,encompassing collagen deposition,angiogenesis,inflammation,and re-epithelialization.Employing electrical stimulation therapy replicates and enhances the effects of endogenous wound electric fields by applying an external electric field to the wound site,thereby promoting skin wound healing.In this study,we developed a self-powered repetitive mechanical impacts-electrical stimulation(RMI-ES)system utilizing a BaTiO_(3)/polydimethylsiloxane(PDMS)piezoelectric composite film.Compared to conventional electrical stimulation devices,the fabricated piezoelectric composite film efficiently harvests energy from the pressure applied by the stimulation device and the tensile force occurring during natural rat activities.The results demonstrated that piezoelectric stimulation generated by the composite membrane expedited the cell cycle,promoting fibroblast proliferation.Additionally,piezoelectric stimulation induced favorable changes in fibroblast gene expression,including increased expression of transforming growth factor-β1(TGF-β1),connective tissue growth factor(CTGF),collagen 1,collagen 3,vascular endothelial growth factor(VEGF),and alpha-smooth muscle actin(α-SMA),while reducing interleukin-6(IL-6)expression.Transcriptome analysis revealed that piezoelectric stimulation may induce fibroblast migration,proliferation,and collagen expression by influencing PI3K/AKT serine/threonine kinase(AKT)pathways.Further confirmation through the addition of the PI3K inhibitor LY294002 validated that piezoelectric stimulation can regulate the repair process after skin injury through the pathway.Importantly,in vivo results demonstrated that the electric field at the wound site effectively promoted wound healing,reduced inflammation,and stimulated collagen deposition and neovascularization.This study emphasizes the role of the piezoelectric membrane as an effective,safe,and battery-free electrical stimulator crucial for skin wound healing.
基金supported by the foundation of the Basic Research Program of Jiangsu Province(Grant No.BK20230112)The Key Research and Development Project of Jiangsu Province(Grant No.BE2019378)+2 种基金The Basic scientific research project of Jiangsu Academy of Agricultural Sciences(Grant No.ZX(18)7003)The High-level Scientific Research Foundation for the introduction of talent of Jinling Institute of Technology(Grant No.jit-b-201914)Jiangsu College students Innovation and entrepreneurship provincial key innovation training program(Grant No.202213573031Z)。
文摘Irrigation and fertilizer interaction is an efficient cultivation management strategy for facility agriculture.However,the effects of irrigation and fertilizer management on tomato growth and its physiological factors remain unclarified.In this study,two irrigation patterns(W1,conventional irrigation;W2,water-saving irrigation)and four fertilizer application patterns(CF,chemical fertilizer;BOF,biological organic fertilizer;NPK,nutrient compound fertilizer;BOF+NPK)were selected to observe the effects of their interaction on cherry tomato plant growth,leaf photosynthesis and fruit quality through pot experiments.The results showed that W2 treatments promoted plant height growth compared to W1 under the same fertilizer addition.Moreover,irrigation and fertilizer management had significant effects on net photosynthetic rate,intercellular oxidation concentration,stomatal conductance and transpiration rate at the first sequence flowering and fruiting stages.The maximum tomato plant height(99.0 cm)was achieved under the irrigation and fertilizer pattern of BOF and W2,along with the highest fruit yield of 1.98 kg/plant,which was approximately 31.1%higher than the minimum yield under the combined CF and W2 treatment.Under W2 treatments,the application of either NPK or BOF increased the soluble sugar content of tomatoes.The structural equation models showed that the soil alkali hydrolyzed nitrogen could directly significantly affect the yield and soluble sugar.The findings suggest that optimization of irrigation-fertilizer interactions positively regulates tomato growth,providing an efficient model for tomato irrigation and fertilizer management and a reference for sustainable development of facility agriculture.
基金financially supported by National Natural Science Foundation of China(NSFC)(22007083)Zhejiang Provincial Innovation Center of Advanced Textile Technology and the Fundamental Research Funds of Shaoxing Keqiao Research Institute of Zhejiang Sci-Tech University(KYY2022004C)the Fundamental Research Funds of Shengzhou Innovation Research Institute of Zhejiang SciTech University(SYY2023B000004)
文摘Building self-assembly nanostructures is an important way to overcome the limitations of paclitaxel in tumor therapy.However,this strategy is also faced with challenges,such as difficulties in efficient release and the potential for drug resistance.Herein,we developed a near-infrared light-activatable melanized paclitaxel self-assembly nanoparticles for synergistic anti-tumor therapy.In this strategy,paclitaxel dimer prodrugs were synthesized and paclitaxel nanoparticles were obtained through self-assembly.Finally,the paclitaxel dimer nanoparticles were capped with polydopamine(PDA,melanoidin)and human serum albumin(HSA).The disulfide bonds in paclitaxel dimeric prodrug specifically respond to high concentrations of glutathione(GSH)and reactive oxygen species(ROS)in tumor cells.PDA enhances the biocompatibility of the drug molecules and imparts near-infrared photothermal conversion capability to the nano-self-assemblies.Both the in vitro and in vivo experiments demonstrated that this paclitaxel nanoprodrug exhibited enhanced tumor therapeutic efficacy under near-infrared light irradiation.
基金financially supported by National Natural Science Foundation of China(NSFC)(22007083)Wenzhou Major Science and Technology Innovation Project(ZZN2023002)+1 种基金the Fundamental Research Funds of Shengzhou Innovation Research Institute of Zhejiang Sci-Tech University(SYY2023B000004)the Fundamental Research Funds of Zhejiang Sci-Tech University(24262132-Y).
文摘Skin aging is a systematic and complex process,mainly including intrinsic aging caused by time change and extrinsic aging caused by environmental factors.Photoaging caused by ultraviolet(UV)irradiation is the principle extrinsic factor of skin aging,which can directly or indirectly cause damage to DNA and other biomolecules,generate reactive oxygen species(ROS),and induce a decrease in collagen synthesis and other adverse effect.Herein,to address the causative factors and multiple side effects of skin photoaging,we constructed multifunctional peptide-melanin hybrid nanodots(PMHDs)to achieve multifaceted synergistic anti-skin aging by using melanin-like nanodots(MDs)and functional peptides as active motifs.Among them,the peptide has the activity of promoting collagen synthesis,while the MDs have the function of absorbing UV light and scavenging free radicals.Additionally,the MDs also act as a carrier to increase the cellular uptake of the peptide.The anti-photoaging effects of the PMHDs were systematically verified in a multidimensional manner at the biochemical,cellular and small animal levels,respectively.
