Houttuynia cordata flavonoid and Sophora japonica L. polysaccharide were investigated to develop anti-UV sun-screening agent through UV wavelength scanning spectrum, antioxidant ability and cell viability after UVB ir...Houttuynia cordata flavonoid and Sophora japonica L. polysaccharide were investigated to develop anti-UV sun-screening agent through UV wavelength scanning spectrum, antioxidant ability and cell viability after UVB irradiation. The results showed that the extracts had strong UVA and UVB absorption ability and hydroxyl free radical scavenging ability. The S. japonica L. polysaccharide showed strong anti-UVB irradiation ability according to the cell viability experiment. It suggests that the H. cordata flavonoid and S. japonica L. polysaccharide could be combined as a candidate of anti-UV sun-screening supplement and compensate for the shortcomings of chemical sunscreens against UVA ray.展开更多
Research on anti-aging activity primarily focuses on crude extracts of anthocyanins,while relatively less attention has been given to anthocyanin monomers.This study was focused on exploring the anti-aging prop-erties...Research on anti-aging activity primarily focuses on crude extracts of anthocyanins,while relatively less attention has been given to anthocyanin monomers.This study was focused on exploring the anti-aging prop-erties of the main anthocyanin monomer,petunidin-3-O-[rhamnopyranosyl-(trans-p-coumaroyl)]-5-O-(β-D-glu-copyranoside)(P3G),from the fruits of Lycium ruthenicum Murray.The effects of P3G on stress endurance,lifespan,and gut microbiota in Caenorhabditis elegans were investigated.It was found that P3G significantly extended the mean lifespan by 19.50%,enhanced fitness through increased motility(30.39%)and pharyngeal pumping(18.33%),and reduced lipofuscin accumulation by 43.61%while maintaining fecundity.P3G pro-longed lifespan by 20.13%and 18.82%during oxidative and heat stress,correspondingly.This effect was attributed to the activation of stress-related genes and the antioxidant system.The transcriptome results showed the enrichment in the longevity pathway and biosynthesis pathway of nematodes.The prominent role of the DAF-16 pathway was highlighted,as P3G enhanced the expression of daf-16 gene and downstream genes(sod-2,sod-3,and mlt-1),promoting nuclear accumulation of DAF-16.Moreover,modifications in the gut microbiota induced by P3G played a role in its anti-aging properties by governing gut microbial composition.Overall,P3G appeared to contribute to promoting the health of nematodes and might help delay their aging to some extent.展开更多
基金Supported by College Students'Practice Innovation Training Project in Henan Province(No.201711834006)Henan Provincial Department of Education(No.15A350008)
文摘Houttuynia cordata flavonoid and Sophora japonica L. polysaccharide were investigated to develop anti-UV sun-screening agent through UV wavelength scanning spectrum, antioxidant ability and cell viability after UVB irradiation. The results showed that the extracts had strong UVA and UVB absorption ability and hydroxyl free radical scavenging ability. The S. japonica L. polysaccharide showed strong anti-UVB irradiation ability according to the cell viability experiment. It suggests that the H. cordata flavonoid and S. japonica L. polysaccharide could be combined as a candidate of anti-UV sun-screening supplement and compensate for the shortcomings of chemical sunscreens against UVA ray.
基金supported by the Key Research and Development Project funded by Ningxia Hui Autonomous Region of China(2021BEF02008)the Priority Academic Program Development of Jiangsu Higher Education Institutions.
文摘Research on anti-aging activity primarily focuses on crude extracts of anthocyanins,while relatively less attention has been given to anthocyanin monomers.This study was focused on exploring the anti-aging prop-erties of the main anthocyanin monomer,petunidin-3-O-[rhamnopyranosyl-(trans-p-coumaroyl)]-5-O-(β-D-glu-copyranoside)(P3G),from the fruits of Lycium ruthenicum Murray.The effects of P3G on stress endurance,lifespan,and gut microbiota in Caenorhabditis elegans were investigated.It was found that P3G significantly extended the mean lifespan by 19.50%,enhanced fitness through increased motility(30.39%)and pharyngeal pumping(18.33%),and reduced lipofuscin accumulation by 43.61%while maintaining fecundity.P3G pro-longed lifespan by 20.13%and 18.82%during oxidative and heat stress,correspondingly.This effect was attributed to the activation of stress-related genes and the antioxidant system.The transcriptome results showed the enrichment in the longevity pathway and biosynthesis pathway of nematodes.The prominent role of the DAF-16 pathway was highlighted,as P3G enhanced the expression of daf-16 gene and downstream genes(sod-2,sod-3,and mlt-1),promoting nuclear accumulation of DAF-16.Moreover,modifications in the gut microbiota induced by P3G played a role in its anti-aging properties by governing gut microbial composition.Overall,P3G appeared to contribute to promoting the health of nematodes and might help delay their aging to some extent.
