Repairing abdominal wall defects presents significant challenges,due to the high infection risk,poor biocompatibility,and insufficient mechanical strength associated with synthetic materials.To overcome these limitati...Repairing abdominal wall defects presents significant challenges,due to the high infection risk,poor biocompatibility,and insufficient mechanical strength associated with synthetic materials.To overcome these limitations,we developed a bioinspired multifunctional 3DPF patch by integrating 3D printing and electrospinning technologies.The core material of the patch is 4arm-PLGA-GPO(4A-GPO),synthesized by conjugating the Gly-Pro-Hyp(GPO)peptide sequence with 4arm-PLGA(4A),which significantly enhances bioactivity and mechanical properties.Additionally,the patch encapsulates basic fibroblast growth factor(bFGF)to stimulate cell proliferation and migration,while an antibacterial layer composes of emodin(EMO)and tobramycin to prevent infection.In vivo studies demonstrate the 3DPF patch effectively accelerates tissue repair by reducing fibrosis and adhesions,promoting angiogenesis and collagen deposition,and modulating the immune response.Transcriptomic analysis reveals that the patch downregulates IL-17 mediated inflammatory pathways while upregulating cell adhesion molecule-related pathways,synergistically facilitating microenvironment reconstruction.Furthermore,molecular docking studies suggest the patch interacts with key molecules such as VEGF and COL3,enhancing angiogenesis and matrix remodeling.In summary,this biomimetic patch,composed of bioactive materials with well-defined chemical compositions,integrates mechanical support,immune modulation,and antibacterial protection.by offering a comprehensive solution for abdominal wall repair,it holds significant potential for clinical translation in complex tissue engineering applications.展开更多
Combined application of biochar with fertilizers has been used to increase soil fertility and crop yield.However,the coupling mechanisms through which biochar improves crop yield at field scale and the time span over ...Combined application of biochar with fertilizers has been used to increase soil fertility and crop yield.However,the coupling mechanisms through which biochar improves crop yield at field scale and the time span over which biochar affects carbon and nitrogen transformation and crop yield are still little known.In this study,a long-term field trial(2013-2019)was performed in brown soil planting maize.Six treatments were designed:CK-control;NPK-application of chemical fertilizers;C1PK-low biochar without nitrogen fertilizer;C1NPK,C_(2)NPK and C_(3)NPK-biochar at 1.5,3 and 6 t ha^(−1),respectively,combined with chemical fertilizers.Results showed that theδ^(15)N value in the topsoil of 0-20 cm layer in the C_(3)NPK treat-ment reached a peak of 291‰at the third year(2018),and demonstrated a peak of 402‰in the NPK treatment in the initial isotope trial in 2016.Synchronously,SOC was not affected until the third to fourth year after biochar addition,and resulted in a significant increase in total N of 2.4 kg N ha^(−1) in 2019 in C_(3)NPK treatment.During the entire experiment,the ^(15)N recovery rates of 74-80%were observed highest in the C_(2)NPK and C_(3)NPK treatments,resulting in an annual increase in yields significantly.The lowest subsoilδ^(15)N values ranged from 66‰to 107‰,and the ^(15)N residual rate would take 70 years for a complete decay to 0.001%in the C_(3)NPK.Our findings suggest that biochar compound fertilizers can increase C stability and N retention in soil and improve N uptake by maize,while the loss of N was minimized.Biochars,therefore,may have an important potential for improving the agroecosystem and ecological balance.展开更多
基金financial support through the Postgraduate Research&Practice Innovation Program of Jiangsu Province[SJCX23-0056]Suzhou Science and Technology Plan Project[SYG202343]Research Fund[GP202410]from Advanced Ocean Institute of Southeast University,Nantong.
文摘Repairing abdominal wall defects presents significant challenges,due to the high infection risk,poor biocompatibility,and insufficient mechanical strength associated with synthetic materials.To overcome these limitations,we developed a bioinspired multifunctional 3DPF patch by integrating 3D printing and electrospinning technologies.The core material of the patch is 4arm-PLGA-GPO(4A-GPO),synthesized by conjugating the Gly-Pro-Hyp(GPO)peptide sequence with 4arm-PLGA(4A),which significantly enhances bioactivity and mechanical properties.Additionally,the patch encapsulates basic fibroblast growth factor(bFGF)to stimulate cell proliferation and migration,while an antibacterial layer composes of emodin(EMO)and tobramycin to prevent infection.In vivo studies demonstrate the 3DPF patch effectively accelerates tissue repair by reducing fibrosis and adhesions,promoting angiogenesis and collagen deposition,and modulating the immune response.Transcriptomic analysis reveals that the patch downregulates IL-17 mediated inflammatory pathways while upregulating cell adhesion molecule-related pathways,synergistically facilitating microenvironment reconstruction.Furthermore,molecular docking studies suggest the patch interacts with key molecules such as VEGF and COL3,enhancing angiogenesis and matrix remodeling.In summary,this biomimetic patch,composed of bioactive materials with well-defined chemical compositions,integrates mechanical support,immune modulation,and antibacterial protection.by offering a comprehensive solution for abdominal wall repair,it holds significant potential for clinical translation in complex tissue engineering applications.
基金This work was supported by the Natural Science Foundation of China(31972511)the National Key Research and Development Program of China(No.2018YFD03003082017YFD0300700).
文摘Combined application of biochar with fertilizers has been used to increase soil fertility and crop yield.However,the coupling mechanisms through which biochar improves crop yield at field scale and the time span over which biochar affects carbon and nitrogen transformation and crop yield are still little known.In this study,a long-term field trial(2013-2019)was performed in brown soil planting maize.Six treatments were designed:CK-control;NPK-application of chemical fertilizers;C1PK-low biochar without nitrogen fertilizer;C1NPK,C_(2)NPK and C_(3)NPK-biochar at 1.5,3 and 6 t ha^(−1),respectively,combined with chemical fertilizers.Results showed that theδ^(15)N value in the topsoil of 0-20 cm layer in the C_(3)NPK treat-ment reached a peak of 291‰at the third year(2018),and demonstrated a peak of 402‰in the NPK treatment in the initial isotope trial in 2016.Synchronously,SOC was not affected until the third to fourth year after biochar addition,and resulted in a significant increase in total N of 2.4 kg N ha^(−1) in 2019 in C_(3)NPK treatment.During the entire experiment,the ^(15)N recovery rates of 74-80%were observed highest in the C_(2)NPK and C_(3)NPK treatments,resulting in an annual increase in yields significantly.The lowest subsoilδ^(15)N values ranged from 66‰to 107‰,and the ^(15)N residual rate would take 70 years for a complete decay to 0.001%in the C_(3)NPK.Our findings suggest that biochar compound fertilizers can increase C stability and N retention in soil and improve N uptake by maize,while the loss of N was minimized.Biochars,therefore,may have an important potential for improving the agroecosystem and ecological balance.
