A class of persistent organic pollutants,polychlorinated biphenyls(PCBs),are ubiquitous in the environment and human tissues which are continuously and long-term threatened.We aim to investigate the toxic effects and ...A class of persistent organic pollutants,polychlorinated biphenyls(PCBs),are ubiquitous in the environment and human tissues which are continuously and long-term threatened.We aim to investigate the toxic effects and the underlying mechanisms of PCB153 using Caenorhabditis elegans as a model organism.Our findings demonstrated that exposure to an optimized concentration of 2μmol/L PCB153 had adverse effects on C.elegans,led to reduction of lifespan,body length,frequency of body bending,and head wiggling.Additionally,this exposure led to an increase in the accumulation of reactive oxygen species,superoxide dismutase,lipofuscin and fat content within the organism.Furthermore,gene set enrichment analysis and whole transcriptome sequencing revealed,a significant up-regulation of Cytochrome P450(CYP)family genes,which are crucial for the xenobiotics metabolism in C.elegans.Knocking down specific genes within the CYPs family,including cyp-35C1,cyp-35A5,cyp-35A3,cyp-34A1,and cyp-34A10,via RNA interference were able to reverse the shortening of lifespan and fat accumulation induced by PCB153 exposure.Moreover,a comprehensive a competing endogenous RNA network was constructed by integrating qPCR-validated differentially expressed m RNA along with lncRNA,circRNA,and miRNA identified by transcriptomic sequencing.This study emphasizes that PCB153 exposure leads to the physiological impairments,particularly fat accumulation and a shorter lifespan,through the modulation of CYP450 family genes.These findings contribute to our understanding of environmental pollutants and their impact on biological systems and provide valuable information on the toxic effects and potential risks associated with PCB153 exposure.展开更多
Carbon and oxygen isotopes of marine carbonate rocks or fossil shells could indicate global sea-level relative change.Generally,heavier carbon isotopes or lighter oxygen isotopes reflected rise of global sea-level rel...Carbon and oxygen isotopes of marine carbonate rocks or fossil shells could indicate global sea-level relative change.Generally,heavier carbon isotopes or lighter oxygen isotopes reflected rise of global sea-level relative changes,and vice versa.For carbonate rocks deposited before Tertiary,carbon isotope was more stable than oxygen isotope,and thus it was more suitable to indicate global sea-level relative fluctuation.Within time limit of the 3rd-order sequence(1-10Ma),large scale migration of carbonate platforms would not occur and carbonate productivity would not change greatly,therefore,a research method of marine tectonic sequence(i.e,a contrastive analysis method of carbon and oxygen isotopessedimentary cycles)was proposed.Thus,the marine 3rd-order sequences were divided the global sealevel sequence which was controlled by global sea-level change and the tectonic sequence which was controlled by regional tectonic subsidence and uplift.The global sea-level fluctuation indicated by carbon and oxygen isotopes were consistent with variation of sedimentary cycles and water depth of the global sea-level sequence,but were not consistent with variation of sedimentary cycles and water depth of the tectonic sequence.As a case study of Ordovician in Tarim Basin,two 3rd-order global sea-level sequences were identified in the Lower Ordovician,while six 3rd-order tectonic sequences controlled by collision and compression between Arkin island arc,Kudi active continental margin uplift and Tarim Plate were identified in the Middle and Upper Ordovician.Particularly,OSQ6 in the Upper Ordovician(Lianglitage Formation)was a typical tectonic sequence.展开更多
基金supported by the National Natural Science Foundation of China(No.81872584)the Natural Science Foundation of Shenzhen(No.JCYJ20210324093211030)+1 种基金Henan Province’s Key R&D and Promotion Projects(No.252102310075)the Interdisciplinary Research for Firstclass Discipline Construction Project of Henan University(No.2019YLXKJC04)。
文摘A class of persistent organic pollutants,polychlorinated biphenyls(PCBs),are ubiquitous in the environment and human tissues which are continuously and long-term threatened.We aim to investigate the toxic effects and the underlying mechanisms of PCB153 using Caenorhabditis elegans as a model organism.Our findings demonstrated that exposure to an optimized concentration of 2μmol/L PCB153 had adverse effects on C.elegans,led to reduction of lifespan,body length,frequency of body bending,and head wiggling.Additionally,this exposure led to an increase in the accumulation of reactive oxygen species,superoxide dismutase,lipofuscin and fat content within the organism.Furthermore,gene set enrichment analysis and whole transcriptome sequencing revealed,a significant up-regulation of Cytochrome P450(CYP)family genes,which are crucial for the xenobiotics metabolism in C.elegans.Knocking down specific genes within the CYPs family,including cyp-35C1,cyp-35A5,cyp-35A3,cyp-34A1,and cyp-34A10,via RNA interference were able to reverse the shortening of lifespan and fat accumulation induced by PCB153 exposure.Moreover,a comprehensive a competing endogenous RNA network was constructed by integrating qPCR-validated differentially expressed m RNA along with lncRNA,circRNA,and miRNA identified by transcriptomic sequencing.This study emphasizes that PCB153 exposure leads to the physiological impairments,particularly fat accumulation and a shorter lifespan,through the modulation of CYP450 family genes.These findings contribute to our understanding of environmental pollutants and their impact on biological systems and provide valuable information on the toxic effects and potential risks associated with PCB153 exposure.
文摘Carbon and oxygen isotopes of marine carbonate rocks or fossil shells could indicate global sea-level relative change.Generally,heavier carbon isotopes or lighter oxygen isotopes reflected rise of global sea-level relative changes,and vice versa.For carbonate rocks deposited before Tertiary,carbon isotope was more stable than oxygen isotope,and thus it was more suitable to indicate global sea-level relative fluctuation.Within time limit of the 3rd-order sequence(1-10Ma),large scale migration of carbonate platforms would not occur and carbonate productivity would not change greatly,therefore,a research method of marine tectonic sequence(i.e,a contrastive analysis method of carbon and oxygen isotopessedimentary cycles)was proposed.Thus,the marine 3rd-order sequences were divided the global sealevel sequence which was controlled by global sea-level change and the tectonic sequence which was controlled by regional tectonic subsidence and uplift.The global sea-level fluctuation indicated by carbon and oxygen isotopes were consistent with variation of sedimentary cycles and water depth of the global sea-level sequence,but were not consistent with variation of sedimentary cycles and water depth of the tectonic sequence.As a case study of Ordovician in Tarim Basin,two 3rd-order global sea-level sequences were identified in the Lower Ordovician,while six 3rd-order tectonic sequences controlled by collision and compression between Arkin island arc,Kudi active continental margin uplift and Tarim Plate were identified in the Middle and Upper Ordovician.Particularly,OSQ6 in the Upper Ordovician(Lianglitage Formation)was a typical tectonic sequence.
