Electrodes underpin electrophysiological signals recording,requiring stable skin contact and low impedance for high-quality,long-term acquisition.Dry microneedle electrodes penetrate the stratum corneum and bypass hai...Electrodes underpin electrophysiological signals recording,requiring stable skin contact and low impedance for high-quality,long-term acquisition.Dry microneedle electrodes penetrate the stratum corneum and bypass hair to ensure robust contact,but conventional rigid designs lack tissue conformity,risking discomfort and injury.This work introduces a modulus-adjustable,mechanically adaptive dry microneedle electrode(MDME)constructed from PEDOT:PSS and shape memory polymer.Submillimeter MDME penetrates skin barriers and,upon body temperature activation,softens to match tissue mechanics,minimizing invasiveness.The MDME exhibits low,stable interface impedance and enables high-quality electromyography,electrocardiography,electroencephalography,and electrocorticography recordings.After one month of usage,the electrophysiological root mean square noise increased by only 6μV,compared to 63μV of Ag/AgCl gel electrodes.Electroencephalogram signal-to-noise reached 8.12 dB versus 7.26 dB for the cranial screw electrodes.This work represents a notable advancement in MDME-based electrophysiological recording,expanding its potential applications in personalized healthcare and human-machine interaction.展开更多
Smart glasses are multifunctional devices designed to replicate the capabilities of smartphones,offering entertainment,fitness tracking,and various other features.However,currently available head-mounted devices tend ...Smart glasses are multifunctional devices designed to replicate the capabilities of smartphones,offering entertainment,fitness tracking,and various other features.However,currently available head-mounted devices tend to be large and heavy,leading to discomfort and diminished immersion.In contrast,the advent of smart contact lenses(SCLs)signifies a significant advancement beyond mere entertainment,representing a groundbreaking intersection of traditional eyewear and advanced scientific innovation.展开更多
Background:Tumor heterogeneity is contributed by tumor cells and the microenvironment.Dynamics of tumor heterogeneity during colorectal cancer(CRC)progression have not been elucidated.Methods:Eight single-cell RNA seq...Background:Tumor heterogeneity is contributed by tumor cells and the microenvironment.Dynamics of tumor heterogeneity during colorectal cancer(CRC)progression have not been elucidated.Methods:Eight single-cell RNA sequencing(scRNA-seq)data sets of CRC were included.Milo was utilized to reveal the differential abundance of cell clusters during progression.The differentiation trajectory was imputed by using the Palantir algorithm and metabolic states were assessed by using scMetabolism.Three spatial transcription sequencing(ST-seq)data sets of CRC were used to validate cell-type abundances and colocalization.Cancer-associated regulatory hubs were defined as communication networks affecting tumor biological behaviors.Finally,quantitative reverse transcription polymerase chain reaction and immunohistochemistry staining were performed for validation.Results:TM4SF1t,SOX4t,and MKI67t tumor cells;CXCL12t cancer-associated fibroblasts;CD4t resident memory T cells;Treg;IgAt plasma cells;and several myeloid subsets were enriched in stage IV CRC,most of which were associated with overall survival of patients.Trajectory analysis indicated that tumor cells from patients with advanced-stage CRC were less differentiated,when metabolic heterogeneity showed a highest metabolic signature in terminal states of stromal cells,T cells,and myeloid cells.Moreover,ST-seq validated cell-type abundance in a spatial context and also revealed the correlation of immune infiltration between tertiary lymphoid structures and tumors followed by validation in our cohort.Importantly,analysis of cancer-associated regulatory hubs revealed a cascade of activated pathways including leukocyte apoptotic process,MAPK pathway,myeloid leukocyte differentiation,and angiogenesis during CRC progression.Conclusions:Tumor heterogeneity was dynamic during progression,with the enrichment of immunosuppressive Treg,myeloid cells,and fibrotic cells.The differential state of tumor cells was associated with cancer staging.Assessment of cancer-associated regulatory hubs suggested impaired antitumor immunity and increased metastatic ability during CRC progression.展开更多
Chronic ophthalmic diseases are multivariate,time-varying,and degenerative.Smart contact lenses have emerged as a scalable platform for noninvasive ocular signal detection and disease diagnosis.However,real-time monit...Chronic ophthalmic diseases are multivariate,time-varying,and degenerative.Smart contact lenses have emerged as a scalable platform for noninvasive ocular signal detection and disease diagnosis.However,real-time monitoring and decoupling of multiple ocular parameters,particularly when the eyes are closed,remain challenging in clinical medicine.In this work,we propose a stretchable bimodal contact lens(BCL)amalgamating self-decoupled electromagnetic capacitive intraocular pressure(CIOP)and magnetic eye movement(MEM)monitoring components.The sandwich-integrated BCL can be intimately attached to the eyeball,enabling closed-eye,wireless,and precise signal acquisition without interference.During the eye open and closed,the serpentine-geometry CIOP unit was validated on a rabbit model,achieving supered resolution(1 mmHg)and sensitivity(≥0.22 MHz mmHg^(-1))for reversible hypo-to hyper-IOP fluctuations.Ex vivo and in vivo MEM monitoring,based on composition-optimized magnetic interlayer film,demonstrated exceptional accuracy(≥97.25%)with eyes open and closed,surpassing existing methods.The collected CIOP and MEM data could be wirelessly aggregated and transmitted to portable devices via integrated acquisition modules within frame glasses for real-time eye healthcare.Emerging noninvasive and bimodal modalities reconcile the trade-off between minimal discomfort,eye status,and reliable measurement,spurring the widespread adoption of the integrated monitoring system for continuous ocular health monitoring.展开更多
近年来,多种新型耐药基因的出现和全球性流行,严重威胁了全球公众健康。CRISPR-Cas9系统(clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9 system)是细菌的一种适应性免疫系统,可切割耐药基因...近年来,多种新型耐药基因的出现和全球性流行,严重威胁了全球公众健康。CRISPR-Cas9系统(clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9 system)是细菌的一种适应性免疫系统,可切割耐药基因、抵御外来核酸入侵,现已作为一种新型基因编辑工具应用于防控细菌耐药性研究。本团队已建立了一种单质粒介导靶向mcr-1基因的CRISPR-Cas9系统,能有效并特异性消除黏菌素耐药大肠杆菌中的mcr-1,恢复其对黏菌素的敏感性。同时也发现在临床中应用还需要优化其递送方式。本文对近几年该技术在细菌耐药性防控方面的研究进展进行了综述,包括CRISPR-Cas9系统的发现过程、作用机制、递送方式、在体外检测实验结果的进展以及当前存在的问题等方面,以期为防控细菌耐药性提供新思路。展开更多
基金supported by the National Natural Science Foundation of China under grant numbers 62422104(G.Y.),U21A20460(Y.L.),62371115(G.Y.),52021001(Y.L.)Science and Technology Major Project of Xizang Autonomous Region of China under grant no.XZ202201ZD0001G(G.Y.)+1 种基金Science and Technology Department of Sichuan Province under grant no.2024NSFSC0234(G.Y.)the Medico-Engineering Cooperation Funds,Fundamental Research Funds for the Central Universities,UESTC under grant nos.ZYGX2020ZB041(G.Y.),ZYGX2021YGLH002(G.Y.).
文摘Electrodes underpin electrophysiological signals recording,requiring stable skin contact and low impedance for high-quality,long-term acquisition.Dry microneedle electrodes penetrate the stratum corneum and bypass hair to ensure robust contact,but conventional rigid designs lack tissue conformity,risking discomfort and injury.This work introduces a modulus-adjustable,mechanically adaptive dry microneedle electrode(MDME)constructed from PEDOT:PSS and shape memory polymer.Submillimeter MDME penetrates skin barriers and,upon body temperature activation,softens to match tissue mechanics,minimizing invasiveness.The MDME exhibits low,stable interface impedance and enables high-quality electromyography,electrocardiography,electroencephalography,and electrocorticography recordings.After one month of usage,the electrophysiological root mean square noise increased by only 6μV,compared to 63μV of Ag/AgCl gel electrodes.Electroencephalogram signal-to-noise reached 8.12 dB versus 7.26 dB for the cranial screw electrodes.This work represents a notable advancement in MDME-based electrophysiological recording,expanding its potential applications in personalized healthcare and human-machine interaction.
基金supported by the National Natural Science Foundation of China(U21A20460,62422104,62371115,and 52021001)the Science and Technology Major Project of Tibetan Autonomous Region of China(XZ202201ZD0001G)+1 种基金the Science and Technology Department of Sichuan Province(2024NSFSC0234)the Medico-Engineering Cooperation Funds,Fundamental Research Funds for the Central Universities(ZYGX2021YGLH002).
文摘Smart glasses are multifunctional devices designed to replicate the capabilities of smartphones,offering entertainment,fitness tracking,and various other features.However,currently available head-mounted devices tend to be large and heavy,leading to discomfort and diminished immersion.In contrast,the advent of smart contact lenses(SCLs)signifies a significant advancement beyond mere entertainment,representing a groundbreaking intersection of traditional eyewear and advanced scientific innovation.
基金supported by the National Key Research and Development Program of China[grant number 2022YFA1304000]the National Natural Science Foundation of China Key Joint Project[grant number U21A20344]+5 种基金the National Natural Science Foundation of China[grant number 81970452]the Program of Guangdong Provincial Clinical Research Center for Digestive Diseases[grant number 2020B1111170004]the Science and Technology Program of Shenzhen,China[grant number JCYJ20190807161807867]the Starting Funding of Faculty from Sun Yat-sen University[grant number 2021276]the Regional Joint Project for Basic and Applied Basic Research Fund of Guangdong Province[grant number 2022A1515111043]the Science and Technology Planning Project of Guangzhou City[grant number 2023A04J01601],and National Key Clinical Discipline.
文摘Background:Tumor heterogeneity is contributed by tumor cells and the microenvironment.Dynamics of tumor heterogeneity during colorectal cancer(CRC)progression have not been elucidated.Methods:Eight single-cell RNA sequencing(scRNA-seq)data sets of CRC were included.Milo was utilized to reveal the differential abundance of cell clusters during progression.The differentiation trajectory was imputed by using the Palantir algorithm and metabolic states were assessed by using scMetabolism.Three spatial transcription sequencing(ST-seq)data sets of CRC were used to validate cell-type abundances and colocalization.Cancer-associated regulatory hubs were defined as communication networks affecting tumor biological behaviors.Finally,quantitative reverse transcription polymerase chain reaction and immunohistochemistry staining were performed for validation.Results:TM4SF1t,SOX4t,and MKI67t tumor cells;CXCL12t cancer-associated fibroblasts;CD4t resident memory T cells;Treg;IgAt plasma cells;and several myeloid subsets were enriched in stage IV CRC,most of which were associated with overall survival of patients.Trajectory analysis indicated that tumor cells from patients with advanced-stage CRC were less differentiated,when metabolic heterogeneity showed a highest metabolic signature in terminal states of stromal cells,T cells,and myeloid cells.Moreover,ST-seq validated cell-type abundance in a spatial context and also revealed the correlation of immune infiltration between tertiary lymphoid structures and tumors followed by validation in our cohort.Importantly,analysis of cancer-associated regulatory hubs revealed a cascade of activated pathways including leukocyte apoptotic process,MAPK pathway,myeloid leukocyte differentiation,and angiogenesis during CRC progression.Conclusions:Tumor heterogeneity was dynamic during progression,with the enrichment of immunosuppressive Treg,myeloid cells,and fibrotic cells.The differential state of tumor cells was associated with cancer staging.Assessment of cancer-associated regulatory hubs suggested impaired antitumor immunity and increased metastatic ability during CRC progression.
基金supported by the National Natural Science Foundation of China under grant numbers 62422104,U21A20460,62371115,52021001Science and Technology Major Project of Tibetan Autonomous Region of China under grant no.XZ202201ZD0001G+1 种基金Science and Technology Department of Sichuan Province under grant no.2024NSFSC0234the Medico-Engineering Cooperation Funds,Fundamental Research Funds for the Central Universities,UESTC under grant nos.ZYGX2020ZB041,ZYGX2021YGLH002.
文摘Chronic ophthalmic diseases are multivariate,time-varying,and degenerative.Smart contact lenses have emerged as a scalable platform for noninvasive ocular signal detection and disease diagnosis.However,real-time monitoring and decoupling of multiple ocular parameters,particularly when the eyes are closed,remain challenging in clinical medicine.In this work,we propose a stretchable bimodal contact lens(BCL)amalgamating self-decoupled electromagnetic capacitive intraocular pressure(CIOP)and magnetic eye movement(MEM)monitoring components.The sandwich-integrated BCL can be intimately attached to the eyeball,enabling closed-eye,wireless,and precise signal acquisition without interference.During the eye open and closed,the serpentine-geometry CIOP unit was validated on a rabbit model,achieving supered resolution(1 mmHg)and sensitivity(≥0.22 MHz mmHg^(-1))for reversible hypo-to hyper-IOP fluctuations.Ex vivo and in vivo MEM monitoring,based on composition-optimized magnetic interlayer film,demonstrated exceptional accuracy(≥97.25%)with eyes open and closed,surpassing existing methods.The collected CIOP and MEM data could be wirelessly aggregated and transmitted to portable devices via integrated acquisition modules within frame glasses for real-time eye healthcare.Emerging noninvasive and bimodal modalities reconcile the trade-off between minimal discomfort,eye status,and reliable measurement,spurring the widespread adoption of the integrated monitoring system for continuous ocular health monitoring.
文摘近年来,多种新型耐药基因的出现和全球性流行,严重威胁了全球公众健康。CRISPR-Cas9系统(clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9 system)是细菌的一种适应性免疫系统,可切割耐药基因、抵御外来核酸入侵,现已作为一种新型基因编辑工具应用于防控细菌耐药性研究。本团队已建立了一种单质粒介导靶向mcr-1基因的CRISPR-Cas9系统,能有效并特异性消除黏菌素耐药大肠杆菌中的mcr-1,恢复其对黏菌素的敏感性。同时也发现在临床中应用还需要优化其递送方式。本文对近几年该技术在细菌耐药性防控方面的研究进展进行了综述,包括CRISPR-Cas9系统的发现过程、作用机制、递送方式、在体外检测实验结果的进展以及当前存在的问题等方面,以期为防控细菌耐药性提供新思路。
