Soil microbiomes play a crucial role in ecosystem functioning,yet knowledge about region-specific bacterial taxa across diverse soil types,particularly at a continental scale,remains uncertain.This study employs 16S r...Soil microbiomes play a crucial role in ecosystem functioning,yet knowledge about region-specific bacterial taxa across diverse soil types,particularly at a continental scale,remains uncertain.This study employs 16S rRNA sequencing to analyze 141 soil samples collected along a 1400 km transect in New South Wales,Australia,revealing distinct bacterial communities adapted to the varying climatic and soil conditions from east to west.The transect is characterised by three unique pedo-climatic zones:>900 mm mean annual precipitation in the eastern region,900300 mm in the central region,and<300 mm in the western region.These variations in climate and soil properties,particularly soil pH,organic carbon,and precipitation,significantly influence bacterial diversity and composition.We identified regionally enriched taxa,including Verrucomicrobia in the eastern,Chloroflexi in the central,and Gemmatimonadetes in the western,demonstrating the adaptation of microbial communities to local environmental conditions.Additionally,our findings show that increasing land use intensity,particularly in agricultural areas,correlates with higher Actinobacteria abundance and leads to more homogenised and interconnected microbial networks.This study provides new insights into the biogeography of soil bacteria in Australia,highlighting the importance of local environmental factors in shaping microbial community structure and offering valuable information for ecological and agricultural management strategies.展开更多
Human disturbances to soils can lead to dramatic changes in soil physical,chemical,and biological properties.The influence of agricultural activities on the bacterial community over different orders of soil and at dep...Human disturbances to soils can lead to dramatic changes in soil physical,chemical,and biological properties.The influence of agricultural activities on the bacterial community over different orders of soil and at depth is still not well understood.We used the concept of genoform and phenoform to investigate the vertical(down to 1 m depth)soil bacterial community structure in paired genosoils(undisturbed forests)and phenosoils(cultivated vineyards)in different soil orders.The study was conducted in the Hunter Valley area,New South Wales,Australia,where samples were collected from 3 different soil orders(Calcarosol,Chromosol,and Kurosol),and each soil order consists of a pair of genosoil and phenosoil.The bacterial community structure was analyzed using highthroughput sequencing of 16S rRNA.Results showed that bacterial-diversity decreased with depth in phenosoils,however,the trend is less obvious in genoform profiles.Topsoil diversity was greater in phenosoils than genosoils,but the trend was reversed in subsoils.Thus,cropping not only affected topsoil bacteria community but also decreased its diversity in the subsoil.Bacterial community in topsoils was influenced by both soil orders and soil forms,however,in subsoils it was more impacted by soil orders.Constrained Analysis of Principal Coordinates revealed that cropping increased the similarity of bacterial structures of different soil orders.This study highlighted the strong influence of agricultural activities on soil microbial distribution with depth,which is controlled by soil order.展开更多
文摘Soil microbiomes play a crucial role in ecosystem functioning,yet knowledge about region-specific bacterial taxa across diverse soil types,particularly at a continental scale,remains uncertain.This study employs 16S rRNA sequencing to analyze 141 soil samples collected along a 1400 km transect in New South Wales,Australia,revealing distinct bacterial communities adapted to the varying climatic and soil conditions from east to west.The transect is characterised by three unique pedo-climatic zones:>900 mm mean annual precipitation in the eastern region,900300 mm in the central region,and<300 mm in the western region.These variations in climate and soil properties,particularly soil pH,organic carbon,and precipitation,significantly influence bacterial diversity and composition.We identified regionally enriched taxa,including Verrucomicrobia in the eastern,Chloroflexi in the central,and Gemmatimonadetes in the western,demonstrating the adaptation of microbial communities to local environmental conditions.Additionally,our findings show that increasing land use intensity,particularly in agricultural areas,correlates with higher Actinobacteria abundance and leads to more homogenised and interconnected microbial networks.This study provides new insights into the biogeography of soil bacteria in Australia,highlighting the importance of local environmental factors in shaping microbial community structure and offering valuable information for ecological and agricultural management strategies.
基金This work was supported by the ARC Discovery project DP190103005 Synergising pedodiversity and soil biodiversity to secure soil functionality。
文摘Human disturbances to soils can lead to dramatic changes in soil physical,chemical,and biological properties.The influence of agricultural activities on the bacterial community over different orders of soil and at depth is still not well understood.We used the concept of genoform and phenoform to investigate the vertical(down to 1 m depth)soil bacterial community structure in paired genosoils(undisturbed forests)and phenosoils(cultivated vineyards)in different soil orders.The study was conducted in the Hunter Valley area,New South Wales,Australia,where samples were collected from 3 different soil orders(Calcarosol,Chromosol,and Kurosol),and each soil order consists of a pair of genosoil and phenosoil.The bacterial community structure was analyzed using highthroughput sequencing of 16S rRNA.Results showed that bacterial-diversity decreased with depth in phenosoils,however,the trend is less obvious in genoform profiles.Topsoil diversity was greater in phenosoils than genosoils,but the trend was reversed in subsoils.Thus,cropping not only affected topsoil bacteria community but also decreased its diversity in the subsoil.Bacterial community in topsoils was influenced by both soil orders and soil forms,however,in subsoils it was more impacted by soil orders.Constrained Analysis of Principal Coordinates revealed that cropping increased the similarity of bacterial structures of different soil orders.This study highlighted the strong influence of agricultural activities on soil microbial distribution with depth,which is controlled by soil order.
